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Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR

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The Journal of Biomedical Research 2020年第5期34卷 387-394页

作者： Xinyu Yu Yuan Wu Jiarong Zhang Jirimutu azhati zulipikaer Jin Chen Center for Global Health School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Medical Oncology Jiangsu Cancer HospitalJiangsu Institute of Cancer Researchthe Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingJiangsu 210009China Key Laboratory of Dairy Biotechnology and Bioengineering Ministry of EducationCollege of Food Science and EngineeringInner Mongolia Agricultural UniversityHohhotInner Mongolia 010018China Xinjiang Academy of Animal Science UrumqiXinjiang 830011China The Key Laboratory of Modern Toxicology Ministry of EducationSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Key Laboratory of Antibody Technique of National Health Commission Nanjing Medical UniversityNanjingJiangsu 211166China

With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations,it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to *** this study,we quantitatively determined the expression levels of T-helper 2(Th2) cytokines in peripheral blood lymphocytes obtained from Bactrian camels by real-time *** recorded kinetic profiles resulting from the immunization of ovalbumin(OVA) indicated that after immunization,Th2 cytokines including interleukin(IL) families such as IL-4,IL-10,and IL-13 in the camels were up-regulated by a factor of 1.78,3.15,and 1.22,respectively,which was validated by traditional enzyme-linked immunosorbent assay(ELISA) *** ELISA which requires specific enzyme-labeled antibodies,this established method based on the minimal amount of blood samples holds an advantage in the preliminary evaluation of camel humoral immune response with desirable precision,which is meaningful for biomedical explorations of camel-derived antibodies.

关键词： Bactrian camels Th2 cytokines humoral immune response real-time PCR

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GSH-responsive curcumin/doxorubicin encapsulated Bactrian camel serum albumin nanocomposites with synergistic effect against lung cancer cells

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The Journal of Biomedical Research 2020年第1期34卷 54-66页

作者： Xinyu Yu Adilijiang Xieripu Qilan Xu azhati zulipikaer Yiyan Song Ling Cai Jin Chen School of Public Health Nanjing Medical UniversityNanjingJiangsu 211166China Xinjiang Academy of Animal Science UrumqiXinjiang 830011China The Key Laboratory of Modern Toxicology Ministry of EducationSchool of Public Health Center for Global Health School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

The aim of this study was to prepare camel serum albumin(CSA) nanoparticles using a self-assembly strategy to co-immobilize curcumin(CCM) and doxorubicin(Dox) which was in favor of combined chemotherapy and biomedical applications of bactrian(Camelus bactrianus) CSA. The constructed CSA nanoparticles(CSA-NPs)with the size around 200 nm displayed a high degree of polydispersity and further encapsulation of CCM and Dox caused no apparent morphological changes to the nanocomposite(CCM/Dox CSA-NPs). The synergistic cytotoxic effect of CCM and Dox on cancer cell A549 was observed with the calculated combination index less than 1.0. Moreover, the release kinetic profile of encapsulated drugs showed a concentration dependence of glutathione(GSH) originating from the GSH used in nanoparticle formation to break the intramolecular disulfide bonds. In vitro cytotoxicity evaluations also revealed that CCM/Dox CSA-NPs showed higher cytotoxicity than that of single drug loaded CSA-NPs, which was also validated by high content screen assay. Taken together, the CCM/Dox CSA-NPs with redox-responsive attributes provided an integrated protein-based combinational drugdelivery matrix to exert synergistic effects.

关键词： camel serum albumin nanoparticles self-assembly redox-responsive synergistic effect

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