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基于抗卷叶病毒棉花基因组研究的基因表达和基因沉默载体的构建

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棉花学报 2002年第Z1期14卷 45-页

作者： shahid MANSOOR

　　Cotton leaf curl virus is the first example of a cotton-infecting virus where infectious clones are available. Plant viruses are valuable tools in understanding plant biology as they can be engineered for expressi...

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水培条件下水稻全生育期耐盐筛选鉴定

植物遗传资源学报 2021年第6期22卷 1567-1581页

作者：张瑞王洋 shahid Hussain 刘永昊邵星宇杨硕陈英龙韦还和戴其根江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室/江苏省粮食作物现代产业技术协同创新中心/扬州大学水稻产业工程技术研究院扬州225009

通过分析水稻在盐胁迫和对照情况下的农艺性状表型差异,筛选出耐盐性强的水稻,为盐碱地的开发利用提供优良种质资源。本研究是水稻全生育期水培耐盐筛选,主要是对国内120份水稻品种进行耐盐性筛选,设置0、1.5‰、3‰、4.5‰和6‰5个盐... 详细信息

通过分析水稻在盐胁迫和对照情况下的农艺性状表型差异,筛选出耐盐性强的水稻,为盐碱地的开发利用提供优良种质资源。本研究是水稻全生育期水培耐盐筛选,主要是对国内120份水稻品种进行耐盐性筛选,设置0、1.5‰、3‰、4.5‰和6‰5个盐浓度梯度,在成熟期时分别测定各个水稻品种的株高、主茎茎粗、根长、倒1叶长、倒1叶宽、倒2叶长、倒2叶宽、绿叶数、主茎穗长、茎干重、叶干重、穗干重、根干重、地上部干重、根冠比、穗数、穗粒数、千粒重、结实率和理论产量。首先利用四分位差法确定本研究的最佳筛选盐浓度;其次对最佳筛选盐浓度下的各个耐盐系数进行主成分分析,在主成分分析基础上通过模糊函数法得到隶属函数值和综合评价值(D值),用D值和各个品种的耐盐系数值进行逐步回归分析和相关分析,最后对各个水稻品种的D值用欧式距离和ward法进行系统聚类分析。结果表明6‰盐浓度是最佳筛选盐浓度,系统聚类分析结果表明在欧式距离为3.5处分类得到11个耐盐性极强的水稻品种,19个耐盐性强的水稻品种,24个耐盐性中等的水稻品种,45个耐盐性弱的水稻品种,21个耐盐性极弱的水稻品种。通过测定盐浓度为6‰时水稻成熟期倒1叶长、倒1叶宽、主茎穗长、地上部干重和主茎茎粗,利用回归方程可快速鉴定水稻的耐盐性大小。通过筛选得到的高耐盐水稻种质资源可以为进一步挖掘耐盐基因和培育水稻耐盐新品种奠定基础。

关键词：水培盐胁迫水稻全生育期筛选

Inexpensive Procedure for Measurement of Ethanol: Application to Bioethanol Production Process

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Advances in Microbiology 2017年第11期7卷 743-748页

作者： Roni Miah Ayesha Siddiqa Jamsheda Ferdous Tuli Noyon Kumar Barman Shuvra Kanti Dey Nihad Adnan Mamoru Yamada Ali azam Talukder Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Biological Chemistry Yamaguchi University Yamaguchi Japan

We have developed a spectrophotometric method for measurement of ethanol concentration in any unknown sample using a solvent tri-n-butyl phosphate [TBP, non-alcoholic solvent, density = 0.975 to 0.976, solubility in water = 0.028% (w/v)]. Solvent TBP separates ethanol from the non-hydrolyzed substrates that could interrupt the desired result by reacting with dichromate reagent. Oxidation of ethanol with dichromate reagent imparts blue green-colour to the solvent, which is easily detected by Spectrophotomer at 595 nm. Our established method showed similar results performed by relatively expensive Gas Chromatography (GC) method. In our present study we put forth a cheap alternate method for determining ethanol concentration in any aqueous solution.

关键词： Bioethanol Yeasts Saccharomyces cerevisiae

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中国传统乳制品中乳杆菌的益生菌潜力评估

食品安全质量检测学报 2020年第21期11卷 8047-8053页

作者： MUHAMMAD shahid-Riaz-Rajoka 赵丽青深圳大学化学与环境工程学院食品科学工程系深圳518060

目的评估从中国传统乳制品中分离出的9株乳杆菌的功能及其益生菌潜力。方法从中国传统乳制品中分离纯化得到乳杆菌菌株,测试其益生菌潜力,如溶血活性和抗氧化活性等。结果所有分离的乳杆菌均可耐受低pH值(pH 2.0、2.5和3.0)和高胆盐条件... 详细信息

目的评估从中国传统乳制品中分离出的9株乳杆菌的功能及其益生菌潜力。方法从中国传统乳制品中分离纯化得到乳杆菌菌株,测试其益生菌潜力,如溶血活性和抗氧化活性等。结果所有分离的乳杆菌均可耐受低pH值(pH 2.0、2.5和3.0)和高胆盐条件(0.3%、0.5%和1%)。分离的乳杆菌对10种抗生素的敏感性,的测试结果表明,所有分离株对万古霉素均没有抗性,此外大多数分离株对青霉素,氨苄青霉素,链霉素和四环素具有抗性。所有乳杆菌对HT-29细胞具有优异的粘附能力,其值介于10.4%~39.0%之间,并且对DPPH自由基和超氧阴离子自由基具有出色的抗氧化活性。结论从中国乳制品中分离出的乳杆菌具有作为各种产品中益生菌的潜力。

关键词：乳杆菌中国传统乳制品益生菌潜力

Changes in gene expression in liver tissue from patients with fulminant hepatitis E

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World Journal of Gastroenterology 2015年第26期21卷 8032-8042页

作者： Anshu Naik Amit Goel Vinita Agrawal Aditya N Sarangi Nanda Chhavi Vineeta Singh shahid Jameel Rakesh Aggarwal Department of Gastroenterology Sanjay Gandhi Postgraduate Institute of Medical Sciences Department of Pathology Sanjay Gandhi Postgraduate Institute of Medical Sciences Sandor Proteomics Pvt Ltd Virology Group International Centre for Genetic Engineering and Biotechnology

AIM: To study host gene expression and numberof immune cells in liver tissues from patients with fulminant hepatitis E(FH-E).METHODS: Microarray-based expression profiling was done using Illumina Human WG-6_v3_Bead Chip arrays on post-mortem liver tissue from 5 patients with FH-E,and compared with similar tissue from 6 patients with fulminant hepatitis B(FH-B; disease controls) and normal liver tissue from 6 *** expression was defined as ≥ 2.0-fold change with Benjamini-Hochberg false discovery rate below 0.05 using t-test in liver tissue from FH-B and FH-E,than healthy liver *** some genes that showed differential expression in FH-E,microarray data were validated using quantitative reverse transcription *** expressed gene lists were then subjected to "Gene Ontology" analysis for biological processes,and pathway analysis using Bio Carta database on the DAVID *** addition,tissue sections were stained for CD4+,CD8+ and CD56+ cells using indirect immunohistochemistry; cells staining positive for each of these markers were counted and compared between ***: Compared to normal livers,those from patients with FH-E and FH-B showed differential expression of 3377 entities(up-regulated 1703,downregulated 1674) and 2572 entities(up 1164,down 1408),*** included 2142(up 896,down 1246) entities that were common between the two sets; most of these belonged to metabolic,hemostatic and complement pathways,which are active in normal *** expression data from livers of patients with FH-E but not those of FH-B showed activation of several immune response pathways,particularly those involving cytotoxic T *** fold-change values of m RNA for selected genes in livers from FH-E than in normal liver tissue determined using quantitative reverse transcription PCR showed excellent concordance with microarray *** immunohistochemistry,CD8+ T cells showed an increase in liver biopsies from both FH-E [median

关键词： Cytotoxic T cells Gene expression HepatitisE Hepatitis E virus Immune response Liver biopsy Microarray Natural killer cells Pathogenesis

Overview of an Emerging Coronavirus Infection, COVID-19: Current Status of Vaccine Development and Therapeutics

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Pharmacology & Pharmacy 2021年第1期12卷 25-41页

作者： Md. Sohel Rana Md. Maruf Hasan Md. Eshak Enan Fatema-Tuz Zohora A. T. M. Zafrul azam Department of Pharmacy Faculty of Pharmacy University of Dhaka Dhaka Bangladesh Department of Pharmacy Primeasia University 9 Banani C/A Dhaka Bangladesh

Coronavirus infectious disease 2019 (COVID-19) first reported in Wuhan, China, causes serious respiratory illnesses such as lung failure and pneumonia. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) the pathogenic agent of COVID-19 has been confirmed as a novel coronavirus. WHO announced COVID-19 a global pandemic and now the whole world is eagerly waiting for vaccines and therapeutic treatment to get rid of this unstoppable coronavirus. As COVID-19 infection, a global threat creates unwanted human casualties and serious economic loss. To stop the ongoing uncontrolled situation researchers are racing to develop prevention and treatment strategies. Vaccines of different countries are in clinical and preclinical trials and the repurposed-drugs are providing to find out a positive result against COVID-19. The report is an analysis of published information focusing

Genome Annotation and Comparative Genomics of ORF Virus

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Advances in Microbiology 2014年第15期4卷 1117-1131页

作者： A. K. M. Firoj Mahmud K. M. Zillur Rahman Shuvra Kanti Dey Tahsina Islam Ali azam Talukder Molecular Biology Department Umea University Umea Sweden Deptartment of Microbiology Jahangirnagar University Savar Bangladesh

ORF virus (ORFV), the etiological agent of contagious pustular dermatitis in small ruminants, belongs to members of the genus Parapoxvirus of the Poxviridae. The genome of the ORFV is dsDNA of 139,962 bp which has about 89% coding region, 63% GC content and codes 130 proteins. There are four unique genes within the genome revealed by homology search of them two posses’ strong regulatory region and transmembrane helices. One of the ORF-039 contains signal peptide indicating the possibilities to be secretory protein coding gene. Comparative genomic analysis reveals significant differences in Bovine Papular Stomatitis Virus (BPSV) strain BV-AR02 and ORFV strain OV-SA00, and these may account for differences in host range. Interspecies sequence variability is observed in all functional classes of genes but is the highest in putative virulence/host range genes. Notably, ORFV contains genes which are homologous of Vaccinia virus. Phylogenetic analysis reveals that although divergent, ORFV virus is distinct from other known mammalian cowpox virus. An improved understanding of Parapoxvirus (PPV) biology will permit the engineering of novel vaccine viruses and expression vectors with enhanced efficacy and greater versatility. The novel vaccine will have a significant role in the economy of a country through the control of disease in an economically important and small ruminant caused by ORFV.

关键词： ORF Virus Contagious Pustular Dermatitis Comparative Genomics GLIMMER ACT Uniue Genes Novel Vaccine

基于互补对称分裂环形谐振器来增强多波段天线带宽的研究（英文）

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真空电子技术 2019年第1期32卷 1-5,26页

作者：安吉阮存军 shahid ULLAH 张幸运电子信息工程学院北京航空航天大学北京100191

本文对采用超材料的概念来提高多波段微带贴片天线的带宽进行了研究。所设计的天线由作为周期谐振器辐射单元的矩形贴片和在地面上蚀刻的3x3互补对称分裂环谐振器(CSSRR)组成。互补对称分裂环谐振器加载的地面通过收集贴片产生的电场来... 详细信息

本文对采用超材料的概念来提高多波段微带贴片天线的带宽进行了研究。所设计的天线由作为周期谐振器辐射单元的矩形贴片和在地面上蚀刻的3x3互补对称分裂环谐振器(CSSRR)组成。互补对称分裂环谐振器加载的地面通过收集贴片产生的电场来增加增益,起到了透镜的作用。文章详细讨论了所提出的周期谐振器天线的等效电路模型,并利用HFSS对结果进行了数值计算。所设计的周期谐振器天线可以工作在C波段和X波段,并且与传统天线相比,具有较高的带宽。

关键词：超材料互补对称分裂环形谐振器微带贴片天线多波段周期谐振器

Automatic Microplot Localization Using UAV Images and a Hierarchical Image-Based Optimization Method

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Plant Phenomics 2021年第1期3卷 99-114页

作者： Sara Mardanisamani Tewodros WAyalew Minhajul Arifin Badhon Nazifa azam Khan Gazi Hasnat Hema Duddu Steve Shirtliffe Sally Vail Ian Stavness Mark Eramian Department of Computer Science University of SaskatchewanSaskatoonSaskatchewanCanada Department of Plant Sciences University of SaskatchewanSaskatoonSaskatchewanCanada Agriculture and Agri-Food Canada SaskatoonSaskatchewanCanada

To develop new crop varieties and monitor plant growth,health,and traits,automated analysis of aerial crop images is an attractive alternative to time-consuming manual *** perform per-microplot phenotypic analysis,localizing and detecting individual microplots in an orthomosaic image of a field are major *** algorithm uses an automatic initialization of the known field layout over the orthomosaic images in roughly the right *** the orthomosaic images are stitched from a large number of smaller images,there can be distortion causing microplot rows not to be entirely straight and the automatic initialization to not correctly position every *** overcome this,we have developed a three-level hierarchical optimization ***,the initial bounding box position is optimized using an objective function that maximizes the level of vegetation inside the ***,columns of microplots are repositioned,constrained by their expected ***,the position of microplots is adjusted individually using an objective function that simultaneously maximizes the area of the microplot overlapping vegetation,minimizes spacing variance between microplots,and maximizes each microplot’s alignment relative to other microplots in the same row and *** orthomosaics used in this study were obtained from multiple dates of canola and wheat breeding *** algorithm was able to detect 99.7%of microplots for canola and 99%for *** automatically segmented microplots were compared to ground truth segmentations,resulting in an average DSC of 91.2%and 89.6%across all microplots and orthomosaics in the canola and wheat datasets.

关键词： breeding spacing entirely