检索结果-南通市图书馆

Possible Effects of Location and Handling on Some Fungi Associated with Rotting Citrullus lanatusThunb. (Watermelon)

引用

American Journal of Plant Sciences 2019年第6期10卷 1061-1068页

作者： ayodele adegboyega sobowale Department of Botany University of Ibadan Ibadan Nigeria

An experiment was conducted to examine probable impact of location and handling on the fungi commonly associated with rotting watermelon (Citrullus lanatus). The rotting watermelon samples were obtained from two vendors each from five different markets (Bodija, Sango, Mokola, Eleyele, Agbowo and Ojoo) in Ibadan, Oyo state. They were placed in different sterile sample bags which were labeled appropriately before bringing them to the Pathology Laboratory, Department of Botany, University of Ibadan. Isolation of fungi from the rotting samples was carried out using pour plate method on acidified Potato Dextrose Agar (APDA). Pathogenicity test was also conducted for the isolated fungi. All experiments were done in duplicates and all plates were incubated at 25°C ± 2°C for 7 days. Pure cultures were obtained and identified and each isolated fungus was recorded as 1 which means present and 0 which means absent. The data obtained were analyzed using SAS version 9.3 at p ≤ 0.05. A total of three fungi were isolated viz., Aspergillus flavus, Saccharomyces cerevisae and Aspergillus niger. Occurrence of A. niger and A. flavus in samples obtained from Bodija, Mokola, Sango and Ojoo markets were significantly (P ≤ 0.05) higher than those from the other two markets. Occurrence of S. cerevisae in samples obtained from Bodija and Mokola markets were significantly (P ≤ 0.05) higher than those from the other three markets. Location had significant impact on the occurrence of isolated fungi in the rotting watermelons. However, handling by vendors had no significant impact on occurrence of the isolated fungi. Vendors and consumers are advised to be mindful of place of purchase and to also maintain good general hygiene before consumption.

关键词： Watermelon A. flavus S. cerevisae A. niger Locations Vendors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Diversity of Fusarium oxysporum and Geotrichum candidum Isolated from Two Tomato Varieties Commonly Consumed in Some Southwestern Town in Nigeria

引用

American Journal of Plant Sciences 2022年第5期13卷 668-685页

作者： ayodele adegboyega sobowale Olalekan Ajibolade Ogunoye Department of Botany University of Ibadan Ibadan Nigeria Department of Crop Soil and Pest Management Technology Rufus Giwa Polytechnic Owo Owo Nigeria

Fusarium oxysporum and Geotrichum candidum, which are among important pathogens of Solanum lycopersicum L. (Tomato), are sometimes misidentified during morphological misidentification. The study was carried out to evaluate molecular diversity of F. oxysporum and G. candidum isolated from two tomato varieties obtained from Akure, Ilorin and Ibadan, Nigeria. The tomato samples were collected and brought back to the laboratory for fungal isolation. Isolation of the pathogens were done following standard procedures. DNA extraction from pure cultures of the pathogens was done at the Centre Laboratory of University of Ibadan. Genetic relationships among the organisms were also estimated by constructing a Dendrogram through UPGMA using the Mega6 Software and genetic distance was computed also using the Mega6 Software. Five strains of F. oxysporum and seven strains of G. candidum were identified. Percentage similarity of the pathogens with those in GenBank was 99.17% - 100.00% for F. oxysporum and 98.48% - 100.00% for G. candidum. The T-01 marker showed the lowest major allele frequency of 0.0833, while T-10 marker has the highest value for major allele frequency of 0.6667 and an average value of 0.3958. Evolutionary relationship showed that the two strains of G. candidum (MN650247 and MN650250) were similar. The three strains of F. oxysporum (MN650246 and MN650248, MN650245 and MN650253) were also similar. Genetic distances among pairs of the fungal strains ranged from 0.12 to 6.30 in pairwise fashion, with an average of 1.32. Evolutionary relationship or closeness among strains of a fungal species can thus be said not to depend on location.

关键词： Fusarium oxysporum Geotricum candidum Genetic Distances Evolutionary Relationship and Genes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Variability of Fusarium verticillioides(Sacc.) in Maize from Three Agro-Ecological Zones of Southwest Nigeria

引用

American Journal of Molecular Biology 2017年第1期7卷 30-40页

作者： Olumayowa Mary Olowe Adeboyega Christopher Odebode Odunayo Joseph Olawuyi ayodele adegboyega sobowale Department of Botany University of Ibadan Ibadan Nigeria

This study investigated the characterisation of Fusarium verticillioides strains using Amplified Fragments Length Polymorphism (AFLP) EcoR1-G/Mse1-C primer combination. This was done to determine the amount of genetic variability present in F. verticillioides population. The objective of this study is to carry out molecular characterization of F. verticillioides strains from infected ear of maize. Six isolates were obtained in maize ear from 10 locations in three agro-ecological zones of Southwest Nigeria. Polymorphic bands detected were 164 from one base extension of EcoR1 and Mse1 primers in the selective amplification. Primer combination EC + MC produced the highest polymorphic bands of 58, while primer combination ET + MC had the highest percentage polymorphism (100%). Genetic similarity (67%) was observed among strains from Ibadan (IBD) (34, 36), Igbooho (IGH) (39), Ado-Ekiti (EKT) (46, 47), Saki (SAK) (52) and Igboora (IGB) (57, 58, 59 and 60). Strains 19 and 20 from Ilesha (ILH) had the highest pairwise similarity coefficient at 97% grouped in cluster II. The dendrogram delineated F. verticillioides strains into four major cluster groups with 77% similarity and other sub-groups within. Fusarium verticillioides strains could therefore be heterogeneous, and thus amplified fragment length polymorphism is an ideal tool for distinguishing the pathogenic variants of Fusarium verticillioides.

关键词： Fusarium verticillioides Amplified Fragment Length Polymorphism Southwest Nigeria Maize

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fungitoxicity of Trichoderma longibrachiatum (Rifai) Metabolites against Fusarium oxysporum, Aspergillus niger and Aspergillus tamarii

引用

American Journal of Plant Sciences 2022年第7期13卷 984-993页

作者： ayodele adegboyega sobowale Linda Chidinma Uzoma Adefoyeke Olufunmilayo Aduramigba-Modupe Bukola Ayodeji Bamkefa Department of Botany University of Ibadan Ibadan Nigeria Nigerian Institute of Science Laboratory Technology (NISLT) Ibadan Nigeria Department of Crop Protection and Environmental Biology University of Ibadan Ibadan Nigeria Department of Biological Sciences Lead City University Ibadan Nigeria

An experiment was carried out to evaluate the fungitoxic effect of Trichoderma longibrachiatum (Rifai) metabolite on F. oxysporum, A. niger and A. tamarii. The fungi were collected from the International Institute of Tropical Agriculture (IITA) and Nigerian Institute of Science Laboratory Technology (NISLT). T. longibrachiatum was cultured on 1/4 strength Potato Dextrose Broth (PDB) following standard procedures. Its metabolite was extracted using 50 ml n-hexane with 50 ml Potato Dextrose Broth (PDB). The metabolite was purified by filter sterilization using a sterile 0.22 millipore filter disc after centrifuging at 900 rpm for 20 minutes. Petri plates of each fungus were later impregnated in triplicates with the T. longibrachiatum metabolites using four concentrations (10%, 25%, 50% and 100%), and three volumes (1 ml, 2 ml, and 3 ml). Petri plates of fungi without the metabolite and Petri Plates of fungi with n-hexane and PDB served as control. All Petri Plates were incubated at 28°C for 7 days. Radial and diametric growth of each fungus on all Petri Plates were taken daily at 24 hours intervals. Data obtained were analysed using SAS (version 9.3). Growth inhibition of F. oxysporum, A. niger and A. tamarii was significantly higher than in control in that order (P ≤ 0.05). Inhibition of the fungi by metabolites extracted with both PDB and n-hexane was significantly better than in control. Generally, inhibition by metabolite extracted with PDB was better than that extracted with n-hexane. Growth inhibition at all the concentrations of the metabolite was significantly better than in the control (P ≤ 0.05). Generally, inhibition at 100%, 50% and 25% concentrations was better than that at 10% concentration of the metabolite. Trichoderma longibrachiatum metabolite can thus be said to possess promising fungitoxic potential even at concentrations as low as 25%. Both PDB and n-hexane are good extraction media for the metabolite.

关键词： Antifungal Metabolites Trichoderma longibrachiatum Fusarium oxysporum Aspergillus niger Aspergillus tamari

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Status of flood vulnerability area in an ungauged basin,southwest Nigeria

引用

International Journal of Agricultural and Biological Engineering 2013年第2期6卷 28-36页

作者： A.sobowale J.A.Oyedepo south west regional center for national water resources capacity building network federal university of agriculturep.m.2.2240abeokuta 110001nigeria institute for food security environmental resources and agricultural researchfederal university of agriculturep.m.2.2240abeokuta 110001nigeria

Many rivers in Nigeria had been ungauged in the last three decades;this worrisome scenario has impacted negatively on the livelihood of people who live in flood *** general lack of up to date streamflow data has made river basin management problematic especially in the area of flood risk management and the development of a real time flood warning *** studies were carried out in the Ogun River Basin in the southwest Nigeria using synthetic data generated by Rainfall-Runoff Modeling using a combination of the Natural Resources Conservation Service(NRCS)curve number model and digital terrain modeling using ArcGIS®9.3 software to identify vulnerable areas in relation to synthesized flood waves generated from the basin,extent of inundation and ranking of the flash points that was equally done using proximity to hazard source as a *** total area in the basin covered by fresh water swamps,salt marsh,and tidal flats at the lower course of Ogun river is 49 km2,while the spatial extent of the entire wetland is 556 km2 *** a peak flood volume of 4270 million m3 generated in September is routed into the fresh water swamp,about 33.4 m of flood depth was left unaccommodated,which will cause inundation of the entire flood plain and severe damage on its *** 1.4 million inhabitants were identified to be at risk in the *** the entire wetland was considered,the flood wave was reduced to an average depth of 8.5 m which is still capable of causing grave damages in the remaining parts of the flood *** flood wave was found to have a residence time of about 45 *** recommendations were given for practical adaptations.

关键词： flood modeling vulnerability management basin Nigeria

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Probable Effects of Dual Inoculation of Maize (Zea mays) Stem with Fusarium verticillioidesand Certain TrichodermaSpecies on Fumonisin Content of Maize Seeds

引用

American Journal of Plant Sciences 2019年第5期10卷 752-759页

作者： A.A.sobowale Department of Botany University of IbadanIbadanNigeria

Seeds from maize (Z. mays) plants whose stems received various treatment combinations of pathogen (F. verticillioides) and four antagonists (i.e. Trichoderma harzianum strain 2, T. hamatum, T. pseudokoningii strains 2 and 5) in the field were subjected to fumonisin analysis. Three pairing methods were employed for the inoculation of pathogen and the antagonists into stem of the maize plant, viz., “Pathogen inoculated before Antagonist”, “Antagonist inoculated before Pathogen”, and “Antagonist and Pathogen inoculated simultaneously”. Controls include “Inoculation of pathogen alone”, “Inoculation of antagonist alone”, and “Inoculation of sterile toothpicks”. Inoculation method used was the toothpick method. Seeds were harvested five weeks after inoculation and subjected to fumonisin analysis. Resulting data were subjected to ANOVA using the GLM procedure of SAS. There was a high significance among treatments i.e. there were varying levels of fumonisin occurrence among the treatments and varying Fusarium occurrences within the blocks. Seeds from treatments involving “Inoculating T. pseudokoningii strain 5 alone” and “Inoculating T. harzianum strain 2 alone” had the highest mean fumonisin content (P > 0.01) which were not significantly higher than in control. Seeds from treatments involving “Inoculating T. pseudokoningii strain 5 and pathogen simultaneously” and “Inoculating T. harzianum strain 2 before pathogen” were significantly low in fumonisin content compared to seeds from other treatments. Seeds which received “Inoculation of T. hamatum alone” were also significantly low (P > 0.01) in fumonisin content compared to others. It could thus be said that treatments involving Trichoderma species applied in the maize stem might have an effect on the fumonisin content and hence Fusarium occurrence in the seeds depending on the occurrence pattern of the Trichoderma within the maize stem.

关键词： Fumonisins Antagonist Trichoderma Species Pathogen Fusarium verticillioides

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Influence of Water Harvesting Practices on Farmer＇s Productivity in Semi-Arid Areas of Nigeria

引用

Journal of Agricultural Science and Technology(A) 2015年第1期5卷 8-14页

作者： Johnson Kayode Adewumi Adeyinka sobowale Are Kolawole Department of Agricultural and Bioresources Engineering Federal University of Agriculture Abeokuta 110001 Nigeria Faculty of Management and Social Sciences Ibrahim Badamosi Babangida University Lapai 911101 Nigeria

Rain water harvesting practices and their effects on the productivity of farming systems in the semi-arid areas of Nigeria have been investigated using participatory rural appraisal （PRA） technique. There was no direct water harvesting interventions by government agencies in the area. Farmers take advantage of naturally existing depressions and abandoned burrow pits close to their farmlands to harvest rain water for surface irrigation using petrol engine pumps. The reservoirs surveyed were found to be grossly inadequate in the drought period. Water budget analysis revealed that evaporation and seepage losses from reservoirs were high in both agro-ecological zone considered. The application of rain water harvesting practices was found to have increased farmers income by 61% and 125% in the Sahel and Sudan Savannah agro-ecological zones, respectively. Governments at all levels in these areas need to support these farmers in order to boost their production to achieve food security in these drought prone areas. Farmers lack timely access to farm credit and agricultural inputs; the indigenous water harvesting practices identified need to be enhanced with the aid of agricultural engineering extension services.

关键词： Rainfall water harvesting irrigation drought productivity fanning systems.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of Soil Water Dynamics in a Tropical Rain Forest Soil （Arinic lixisol）, Abeokuta, Nigeria

引用

Journal of Agricultural Science and Technology(A) 2014年第6期4卷 494-503页

作者： sobowale Adeyinka Adewumi Johnson Kayode Ogunniranye Gabriel Bayo Sajo Opeyemi Samuel Department of Agricultural Engineering Federal University of Agriculture Abeokuta 110001 Ogun State Nigeria Department of Agriculture Joseph Ayo Babalola University llesha 233121 Osun State Nigeria

Soil water dynamics in the dominant lwo soil series （Arinic lixisol） were evaluated at the Federal University of Agriculture, Alabata, Abeokuta, Nigeria. Field capacity, infiltration and water retention characteristics were evaluated in situ for a period of 161 d in the dry season for two root zone depths. Results show that the Iwo soil series has a field capacity ranging from 2.6%-5.5% at 0-45 cm and 45-90 cm root zone depths, respectively. The soil is quick draining with high infiltration rate and very poor water retention capacity confirming that the soil will require a short irrigation interval of about 2-3 d since available water for plant growth in predominantly sandy soils ranges between 2%-8%. Based on the foregoing, sprinkler irrigation is best suited for the lwo soil series, it should, however, be noted that the water application rate must be less than the infiltration rate of the soil in order to prevent surface ponding and runoff. A multivariate model relating soil moisture content with soil moisture tension and soil temperature calibrated within the study had very low model accuracy of 56% and 45% for the two root zone depths, respectively, implying the need for further studies.

关键词： Soil water infiltration water retention soil tension irrigation root zone depth.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Correlation and Path Coefficient Analyses of Yield in Cacao(Theobroma cacao L.)

引用

Journal of Botanical Research 2022年第3期4卷 1-8页

作者： Omotayo Olalekan Adenuga Abigail Funlayo Adepoju Ibrahim Olalekan sobowale Olayinka Olufemi Olaniyi Oluwatobi James Areola Terkula Felix Nyamkyume Plant Breeding Department Cocoa Research Institute of NigeriaIbadan23401Nigeria

Cacao(Theobroma cacao L.)is an important commodity tree crop which produces the cocoa bean,a major source of income for most West African countries and many smallholder *** yield of cacao is a major limitation to cocoa production in *** study aimed at determining the correlations of the phenotypic traits that were related in the yield of the cacao *** cacao hybrids produced from some high-yielding parents in the research farm of Cocoa Research Institute of Nigeria,Ibadan,Nigeria were evaluated from 2012 through 2017 in Owena(7°11’N,5°1’E),Ondo state,*** Correlations and Path Coefficient Analysis were used in the description of the performance of the *** study concluded that significant genotypic and phenotypic correlations existed among many of the pairs of the fruit and bean characters with one another and with pod index,suggesting a complex contribution of these characters either positively or negatively to growth and yield in cacao,and that fruit and bean traits are determinants of yield in cacao.

关键词： Cacao Yield Correlation Phenotypic traits Path coefficients

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：