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支持策略与属性全隐藏的CP-ABE方案

计算机科学 2024年第12期51卷 317-325页

作者：姜露寒田有亮向阿新贵州大学公共大数据国家重点实验室贵阳550025 贵州大学计算机科学与技术学院贵阳550025 贵州大学密码学与数据安全研究所贵阳550025 贵州省密码学与区块链技术特色重点实验室贵阳550025

已有的支持策略或属性隐藏的CP-ABE方案可实现隐私保护的细粒度访问控制,但大部分方案仅实现了关于属性值的部分策略隐藏,且忽略了密钥生成过程的用户属性隐藏问题,仍易造成用户隐私信息泄露。针对上述问题,文中提出了一种完全隐藏访问... 详细信息

已有的支持策略或属性隐藏的CP-ABE方案可实现隐私保护的细粒度访问控制,但大部分方案仅实现了关于属性值的部分策略隐藏,且忽略了密钥生成过程的用户属性隐藏问题,仍易造成用户隐私信息泄露。针对上述问题,文中提出了一种完全隐藏访问策略和用户属性的CP-ABE方案,用于数据访问控制和密钥生成过程中的用户隐私信息保护。首先,提出了属性莫顿过滤器(Attribute Morton Filter,AMF),加密阶段将访问策略完全隐藏于AMF中,解密阶段用户可高效查询并精准判断用户属性在策略中的位置;其次,提出了一种基于zk-SNARKs的密钥生成方法,有效隐藏了密钥生成过程中的用户属性;最后,安全性证明及性能分析表明,所提方案在不影响效率的同时具有选择明文攻击下的不可区分性。

关键词：属性基加密访问策略用户属性完全隐藏属性莫顿过滤器

Surface engineering of Li-and Mn-rich layered oxides for superior Li-ion battery

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Tungsten 2024年第1期6卷 259-268页

作者： Lu-xiang Ma Tian-Dong Chen Chun-Xi Hai Sheng-De Dong Xin He Qi Xu Hang Feng axin Ji-Tao Chen Yuan Zhou College of Materials and Chemistry and Chemical Engineering Chengdu University of TechnologyChendu 610000China Qinghai Building and Materials Research Co. LtdXining 810000China Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

The Li-and Mn-rich layered oxides(R-LNCM)are considered as promising cathode materials for high-energy density lithium-ion batteries(LIBs).However,the interface side reaction aggravates the voltage and capacity fading between cathode material and electrolyte at high voltage,which severely hinders the practical application of LIB ***,lithium polyacrylate(LiPAA)as the binder and coating agent is applied to suppress the voltage and capacity fading of R-LNCM *** flexible LiPAA layers with high elasticity are capable of impeding cathode cracks on the particle surface via mechanical stress ***,superior voltage and capacity fading suppression on R-LNCM electrode is finally *** a result,LiPAA-R-LNCM cathode exhibits a remarkable specific capacity of 186 mA·h·g^(-1)with~73%retention at 1℃after ***,the corresponding average discharge potential is maintained to~3.1 V with only~0.4 V falling.

关键词： LiPAA interphase layer Interface side reaction Cathode cracks Capacity and voltage fading suppression Liand Mn-rich cathode

细胞周期蛋白依赖性激酶7抑制剂THZ1对脑胶质瘤干细胞干性的调控及机制

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中国组织工程研究 2025年第25期29卷 5374-5381页

作者：虎恩喜何文莹陶翔杜沛静王立斌宁夏医科大学第一临床医学院宁夏回族自治区银川市750004 宁夏医科大学基础医学院宁夏回族自治区银川市750004 华中科技大学协和深圳医院广东省深圳市518000

背景:THZ1是一种细胞周期蛋白依赖性激酶7的抑制剂,可以抑制多种肿瘤细胞的增殖,但THZ1是否可以通过Wnt/β-catenin信号通路影响脑胶质瘤干细胞的干性尚不清楚。目的:探究THZ1对脑胶质瘤细胞U87干性的影响及机制。方法:培养脑胶质瘤细胞... 详细信息

背景:THZ1是一种细胞周期蛋白依赖性激酶7的抑制剂,可以抑制多种肿瘤细胞的增殖,但THZ1是否可以通过Wnt/β-catenin信号通路影响脑胶质瘤干细胞的干性尚不清楚。目的:探究THZ1对脑胶质瘤细胞U87干性的影响及机制。方法:培养脑胶质瘤细胞U87形成干细胞球并通过Western blot验证干性相关蛋白的表达;采用CCK-8法检测THZ1作用于U87细胞的半数抑制浓度(IC_(50));通过克隆实验、划痕实验、Transwell迁移实验确定THZ1对U87细胞增殖、迁移的影响;分析THZ1处理对U87干细胞成球率及干细胞球体大小的影响;Western blot检测干性相关蛋白CD133、ABCG2、Nanog、OCT4、SOX2和上皮-间充质转化相关蛋白E-cadherin、N-cadherin、Occludin、Snail以及Wnt/β-catenin通路相关蛋白axin1、β-Catenin、Wnt-5a、GSK3β、Cyclind-1、C-myc的表达变化。结果与结论:①与贴壁细胞相比,U87干细胞干性相关蛋白Nestin、CD133、ABCG2、Nanog、OCT4、SOX2表达明显升高;②与对照组相比,THZ1减弱了U87细胞的增殖和迁移能力;③THZ1抑制U87干细胞成球率及球体大小,下调干性相关蛋白的表达;④THZ1处理后,U87干细胞中N-cadherin、Snail蛋白表达降低,而E-cadherin、Occludin蛋白表达升高;(5)THZ1处理使U87干细胞中Wnt/β-catenin通路相关蛋白axin1、β-Catenin、Wnt-5a、GSK3β、Cyclind-1、C-myc表达降低。结果表明:THZ1通过下调Wnt/β-catenin信号通路相关分子表达,抑制脑胶质瘤细胞U87的增殖、迁移,抑制U87干细胞成球能力、干性相关蛋白表达和上皮-间充质转化能力。

关键词： THZ1 脑胶质瘤肿瘤干细胞成球实验干性标志物 Wnt/β-catenin信号通路上皮-间充质转化增殖迁移

Inhibition of GSK-3β ameliorates hepatic ischemia-reperfusion injury through GSK-3β/ β-catenin signaling pathway in mice

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 278-284页

作者： Yong-xiang Xia, Ling Lu, Zheng-Shan Wu, Li-Yong Pu, Bei-Cheng Sun and Xue-Hao Wang Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China Liver Transplantation Center First Affiliated Hospital Nanjing Medical University Nanjing 210029 China

BACKGROUND: Glycogen synthase kinase (GSK)-3β/β-catenin signaling regulates ischemia-reperfusion (I/R)-induced apoptosis and proliferation, and inhibition of GSK-3β has beneficial effects on I/R injury in the heart and the central nervous system. However, the role of this signaling in hepatic I/R injury remains unclear. The present study aimed to investigate the effects and mechanism of GSK-3β/β-catenin signaling in hepatic I/R injury. METHODS: Male C57BL/6 mice (weighing 22-25 g) were pretreated with either SB216763, an inhibitor of GSK-3β, or vehicle. These mice were subjected to partial hepatic I/R. Blood was collected for test of alanine aminotransferase (ALT), and liver specimen for assays of phosphorylation at the Ser9 residue of GSK-3β, GSK-3β activity, axin 2 and the anti- apoptotic factors Bcl-2 and survivin, as well as the proliferative factors cyclin D1 and proliferating cell nuclear antigen, and apoptotic index (TUNEL). Real-time PCR, Western blotting and immunohistochemical staining were used. RESULTS: SB216763 increased phospho-GSK-3β levels and suppressed GSK-3β activity (1880±229 vs 3280±272 cpm, Paxin 2. Upregulation of GSK-3β/β-catenin signaling increased Bcl-2, survivin and cyclin D1. Serological and histological analyses showed thatSB216763 alleviated hepatic I/R-induced injury by reducing apoptosis (1.4±0.2% vs 3.6±0.4%, P<0.05) and enhanced liver proliferation (56±8% vs 19±4%, P<0.05). CONCLUSION: Inhibition of GSK-3β ameliorates hepatic I/R injury through the GSK-3β/β-catenin signaling pathway.

关键词： glycogen synthase kinase 3 beta-catenin reperfusion injury signal transduction

创伤骨科APP软件研发与应用对医学本科生创新能力培养的作用

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中华医学教育探索杂志 2015年第9期14卷 920-923页

作者：黄安祺金丹黄树毅庒夏鑫付苏相大勇佟旷陈庆南方医科大学南方医院创伤骨科广州510515 南方医科大学南方医院外科教研室广州510515 南方医科大学教务处

针对医移动信息学本科生创新能力培养的现状，组建以医学本科生为主体的创伤骨科移动医疗软件制作团队，进行骨折分型与功能评分、足踝部损伤康复软件研发。以软件制作和软件推广试用的方式在教学和临床领域分别进行实践，促使学生对软... 详细信息

针对医移动信息学本科生创新能力培养的现状，组建以医学本科生为主体的创伤骨科移动医疗软件制作团队，进行骨折分型与功能评分、足踝部损伤康复软件研发。以软件制作和软件推广试用的方式在教学和临床领域分别进行实践，促使学生对软件制作过程中所涉及的专业知识进行学习。本项目实施效果良好，已研发出获得计算机著作产权的、临床应用便捷的相关移动医疗软件。通过软件制作过程中所涉及的多个环节的锻炼，有效激发了学生的创新意识．培养了拥有多元创新能力的复合型医学人才。

关键词：创伤骨科移动医疗软件医学本科生创新能力

Integrative analysis of aberrant Wnt signaling in hepatitis B virus-related hepatocellular carcinoma

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World Journal of Gastroenterology 2015年第20期21卷 6317-6328页

作者： Shan-Long Ding Zi-Wei Yang Jie Wang Xiao-Lei Zhang xiang-Mei Chen Feng-Min Lu Department of Microbiology and Infectious Disease Center School of Basic Medical SciencesPeking University Health Science Center

AIM: To comprehensively understand the underlying molecular events accounting for aberrant Wnt signaling activation in hepatocellular carcinoma(HCC).METHODS: This study was retrospective. The HCC tissue specimens used in this research were obtained from patients who underwent liver surgery. The Catalogue of Somatic Mutations in Cancer(COSMIC) database was searched for the mutation statuses of CTNNB1, TP53, and protein degradation regulator genes of CTNNB1. Dual-luciferase reporter assay was performed with TOP/FOP reporters to detect whether TP53 gain-of-function(GOF) mutations could enhance the transcriptional activity of Wnt signaling. Methylation sensitive restriction enzyme-quantitative PCR was used to explore the methylation status of Cp G islands located in the promoters of APC, SFRP1, and SFRP5 in HCCs with different risk factors. Finally, nestedreverse transcription PCR was performed to examine the integration of HBx in front of LINE1 element and the existence of HBx-LINE1 chimeric transcript in Hepatitis B virus-related HCC. All results in this article were analyzed with the software SPSS version 19.0 for Windows, and different groups were compared by χ2 test as ***: Based on the data from COSMIC database, compared with other solid tumors, mutation frequency of CTNNB1 was significantly higher in HCC(P axin1 were mutated in HCC. By using methylation sensitive restriction enzym

关键词： β-catenin CTNNB1 Hepatitis B virus Hepatocellular carcinoma TP53 Wnt signaling

Modulation of Wnt/β-catenin signaling affects the directional differentiation of hair follicle stem cells

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Plastic and Aesthetic Research 2016年第1期3卷 39-46页

作者： Bin Yang Xiao-Ying Wu Jian Ni Bing-Hang Li Li-Huan Deng Meng-Juan xiang Plastic Surgery Hospital and Institute Chinese Academy of Medical SciencesPeking Union Medical CollegeShijingshanBeijing 100144China

Aim:The differentiation of hair follicle stem cells(HFSCs)into hair follicle cells has potential clinical applications for cutaneous ***,the mechanisms regulating the differentiation of HFSCs into hair follicular papilla or epidermal cells are currently not *** study investigated the role of the Wnt/β-catenin pathway and its crosstalk with other signaling components during this differentiation ***:Lithium chloride(LiCl,10 mmol/L)and keratinocyte growth factor(KGF,10μg/L)were used to induce HFSC differentiation,validated by immunofluorescence *** mRNA expression ofβ-catenin,adenomatous polyposis coli,glycogen synthase kinase-3β(GSK-3β),axin,and lymphoid enhancer factor-1 after 3,5,7,and 9 days were measured to evaluate the role of the Wnt/β-catenin ***:During LiCl-induced HFSC differentiation into hair follicle cells,the Wnt/β-catenin signaling pathway was activated and the expression of GSK-3β,a vital component of the degradation compound,was *** led to increased cytoplasmicβ-catenin expression,nuclear translocation,and subsequent target gene *** contrast,KGF induced the differentiation of HFSCs into epidermal cells and did not affect the expression ofβ-*** data indicates that LiCl and KGF distinctly regulate the differentiation of HFSCs into hair follicle and epidermal cells,***,the Wnt/β-catenin signaling pathway is predominantly involved in hair follicle ***:these results demonstrate that LiCl can be used to differentiate HFSCs into hair follicle cells in vitro,which has important therapeutic applications for treating patients with cutaneous damage.

关键词： Lithium chloride keratinocyte growth factor hair follicle stem cells Wnt/β-catenin signaling pathway

毛囊干细胞定向分化过程中Wnt信号通路介导的基因调控

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组织工程与重建外科杂志 2011年第5期7卷 290-294页

作者：向萌娟杨斌广州医学院广东省广州市510182 协和医学院中国医学科学院整形外科医院北京市100144

皮肤的毛囊干细胞具有自我复制以及多向分化潜能,在毛囊形态发育和定向分化过程中,Wnt信号通路起决定性作用。参与这条信号通路的重要蛋白质,如Wnt蛋白、Frizzled、β-catenin、GSK3β、APC、axin等研究相对较早,且颇为深入。但对于这... 详细信息

皮肤的毛囊干细胞具有自我复制以及多向分化潜能,在毛囊形态发育和定向分化过程中,Wnt信号通路起决定性作用。参与这条信号通路的重要蛋白质,如Wnt蛋白、Frizzled、β-catenin、GSK3β、APC、axin等研究相对较早,且颇为深入。但对于这条通路下游的调节因子,尤其是细胞核内关键性转录因子Tcf3、Lef1,以及它们所调控的一些重要基因c-myc、cyclinD1等的研究仍处于起步阶段。本文就Wnt信号通路介导的基因调节毛囊干细胞定向分化的研究现状进行综述,为构建组织工程皮肤提供理论参考。

关键词：毛囊干细胞 Wnt信号通路基因调控

A novel peptide stapling strategy enables retention of ring-closing amino acid side chains for Wnt/β-catenin signalling pathway

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A novel peptide stapling strategy enables retention of ring-...