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Heterotrophic Bacterial Floras in Industrial Area and Unpolluted Marine Environments around Qingdao

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Journal of Ocean University of Qingdao 2002年第1期1卷 59-62页

作者： DUZongjun LIYun YUDehua WANGXianghong CHENJixiang RobertsonP.A.W austinb XUHuaishu CollegeofMarineLifeSciences OceanUniversityofQingdaoQingdao266003P.R.China DepartmentofBiologicalSciences Heriot-WattUniversityEdinburghEH144ASUK

From Oct. 1999 to Oct. 2000, the heterotrophic bacterial floras in the industrial marine environment around the Qingdao Power Plant (QPP) and in the unpolluted marine environments were investigated. The results showed that the numbers of the heterotrophic bacteria around QPP were much higher than those in unpolluted environments, and the average numbers in QPP Seawater, QPP Sediment, Unpolluted Seawater and Unpolluted Sediment were 5.4×104cfu(mL) -1, 5.0×105cfug -1, 3.0×102cfu(mL) -1 and 1.3×105cfug -1 respectively. Totally, 118 strains were isolated from QPP and 99 of them were Gram-negative. One hundred and twenty one strains were isolated from the unpolluted environments and 104 of them were Gram-negative. All the Gram-negative bacteria belonged to 13 genera. The distribution of the bacteria was varied in different marine environments. The results showed that the unpolluted marine environments contained much more Vibrio than seawater and sediment around QPP.

关键词： marine environments heterotrophic bacteria bacterial floras Qingdao

Heterotrophic Bacterial Flora in Aquaculture Area around Xuejiadao

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Journal of Ocean University of Qingdao 2002年第2期1卷 157-160页

作者： DUZongjun LIYun YUDehua WANGXianghong CHENJixiang RobertsonP.A.W. austinb. XUHuaishu CollegeofMarineLifeSciences OceanUniversityofQingdaoQingdao266003P.R.China DepartmentofBiologicalSciences Heriot-WattUniversityEdinburghEH144ASU.K.

From Oct., 1999 to Oct., 2000, the heterotrophic bacterial flora in the aquaculture area around Xuejiadao was investigated. The result shows that the populations of the heterotrophic bacteria are heavier in summer and autumn than those in winter and spring. The average populations in seawater, sediment, the surface of seaweed and the surface of fish are 1.4×10 4 cfu mL -1, 5.4×10 6 cfu g -1, 1.5×10 6 cfu g -1 and 1.8×10 3 cfu cm -2, respectively. A total of 301 strains were isolated, among them 259 were Gram-negative. All the Gram-negative bacteria belong to 13 genera and some genera of Enterobacteriaceae. The communities of bacteria are slightly different among the samples. In the body surface of fish, Genus vibrio is dominant. In the remaining samples, dominant genus is Aeromonas.

关键词： heterotrophic bacteria bacterial flora aquaculture

Methods Used to Study Bacterial Diversity in the Marine Environment around Qingdao

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Journal of Ocean University of Qingdao 2002年第2期1卷 153-156页

作者： RobertsonP.A.W MaclnnesJ SparaganoO.A.E PurdomI LIY YUD.H DUZ.J XUH.S austinb CentreforMarineBiodiversityandBiotechnology（CMBB） DepartmentofBiologicalSciencesHeriot-WattUniversityRiccartonEdinburgh.EH144ASScotlandUK TheDarwinUnit DepartmentofMarineBiologyOceanUniversityofQingdaoQingdao266003P.R.China

Pollution has a considerable effect on biological communities, in terms of size and diversity of the populations. Yet, the precise consequences of human activity on microbial communities in the marine environment are poorly understood. Therefore, in an ongoing collaborative research programme between Heriot-Watt University and the Ocean University of Qingdao, bacteria were isolated in 1999 and 2000 from marine sediment, seawater, seaweed, fish and shellfish, taken from locations in Shandong Province adjacent to Qingdao. Sampling locations were comprised of industrial and aquacultural sites and a clean, control site. In order to analyse microbial diversity, a polyphasic approach was adopted for characterisation of these isolates, specifically through examination of key phenotypic traits, i.e. using Biolog GN MicroPlate TM profiles, bacterial whole cell protein profiles and 16S and 23S rRNA gene sequences. These techniques yielded complex taxonomic data, which were subjected to statistical and cluster analyses. The application of these methods to studies of microbial communities is discussed.

关键词： bacteria marine environment biodiversity Biolog GN