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西瓜基因组框架图谱绘制与20份代表性资源重测序研究

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中国瓜菜 2019年第8期32卷 193-195页

作者：郭绍贵(编译) 许勇(编译) 张建国孙宏贺 Jerome Salse William JLucas 张海英 Yi Zheng 毛林勇任毅王志文闵久梦郭晓森 Florent Murat Byung-Kook Ham 张召亮高山黄明云徐益民钟思林 aureliano bombarely Lukas A Mueller 赵泓何洪巨张岩张忠华黄三文谭涛庞尔丽林魁胡群匡汉辉倪培相王博刘景安孔秋生侯文菊邹小花江姣宫国义 Kathrin Klee Heiko Schoof 黄莹胡雪松董珊珊梁德全王娟吴奎夏阳赵翔郑泽群邢淼梁新明黄邦清吕添王军一尹烨伊鸿平李瑞强吴明珠 Amnon Levil 张兴平 James J Giovannoni 王俊李云伏费章君北京市农林科学院蔬菜研究中心国家蔬菜工程技术研究中心·北京市农林科学院·农业部华北地区园艺作物生物学与种质创制重点实验室康奈尔大学博伊斯汤普逊研究所农业部基因组学重点实验室·深圳华大基因研究院北京农学院植物科学技术学院法国农业科学研究院联合研究中心谷类遗传学多样性和生态生理研究所加州大学生物科学学院植物生物学系(戴维斯) 中国农业科学院蔬菜花卉研究所北京师范大学生命科学学院华中农业大学园林学院波恩大学植物科学与资源保护研究所作物生物信息研究室新疆农业科学院北京诺禾致源生物信息科技有限公司美国农业部农业科学研究院蔬菜研究室美国农业部农业与健康罗伯特霍利研究中心哥本哈根大学生物系复旦大学理论生命科学研究中心

目的与意义:西瓜是一种重要的葫芦科作物,占全世界蔬菜生产面积的7%.经过长期人工选育,西瓜遗传多样性变的十分狭窄,已成为西瓜品种改良的瓶颈.本研究以东亚类型高品质西瓜品种‘97103’为材料进行西瓜全基因组序列分析,并开展20份代表... 详细信息

目的与意义:西瓜是一种重要的葫芦科作物,占全世界蔬菜生产面积的7%.经过长期人工选育,西瓜遗传多样性变的十分狭窄,已成为西瓜品种改良的瓶颈.本研究以东亚类型高品质西瓜品种‘97103’为材料进行西瓜全基因组序列分析,并开展20份代表性西瓜资源的基因组重测序分析,以明确西瓜基因组结构和进化历程,阐明果实品质和维管束信号传导等重要生物学过程的分子机制.

关键词：全基因组测序分析西瓜资源图谱框架品种改良果实品质

Recent perspectives into biochemistry of decavanadate

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World Journal of Biological Chemistry 2011年第10期2卷 215-225页

作者： Manuel aureliano FCT University of Algarve Gambelas 8005-139 Faro Portugal

The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V10) clearly differs from monomeric vanadate (V1), and affects differently calcium pumps, and structure and function of myosin and actin. Only decavanadate inhibits calcium accumulation by calcium pump ATPase, and strongly inhibits actomyosin ATPase activity (IC50 = 1.4 μmol/L, V10), whereas no such ef- fects are detected with V1 up to 150 μmol/L; prevents actin polymerization (IC50 of 68 μmol/L, whereas no effects detected with up to 2 mmol/L V1); and interacts with actin in a way that induces cysteine oxidation and vanadate reduction to vanadyl. Moreover, in vivo decavanadate toxicity studies have revealed that acute exposure to polyoxovanadate induces different changes in antioxidant enzymes and oxidative stress parameters, in comparison with vanadate. In vitro studies have clearly demonstrated that mitochondrial oxygen consumption is strongly affected by decavanadate (IC50, 0.1 μmol/L); perhaps the most relevant biological effect. Finally, decavanadate (100 μmol/L) increases rat adipocyte glucose accumulation more potently than several vanadium complexes. Preliminary studies sug- gest that decavanadate does not have similar effects in human adipocytes. Although decavanadate can be a useful biochemical tool, further studies must be carried out before it can be conf irmed that decavanadate and its complexes can be used as anticancer or antidiabetic agents.

关键词： Decavanadate Vanadate Calcium pump Myosin Actin Actin polymerization Insulin mimetic Antidiabetic agent Antitumor agent

General and Exact Inbreeding Coefficient of Maize Synthetics Derived from Three-Way Line Hybrids

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Phyton-International Journal of Experimental Botany 2022年第1期91卷 33-43页

作者： Alejandro Ibarra-Sánchez Juan Enrique Rodríguez-Pérez aureliano Peña-Lomelí Clemente Villanueva-Verduzco Jaime Sahagún-Castellanos Crop Science Department Chapingo Autonomus UniversityChapingo56230México

Synthetic varieties(SVs)are populations generated by randomly mating their *** are a good alternative for low-input farmers who grow onions,maize,and other allogamous crops since the seed produced by a SV does not change from one generation to the *** SV progenitors are commonly pure lines,in this case a synthetic(Syn_(TC))whose parents are t three-way line crosses,a very common type of maize hybrid grown in Mexico,is *** aim was to develop a general and exact equation for the inbreeding coefficient of a Syn_(TC)eF_(m)^(f)Syn_(TC)T because of its relationship with the mean of economically important *** objective arose due to the need for a more advanced study in terms of determining whether F_(m)^(f)Syn_(TC)can be applied specifically and accurately for any number of parents(t),plants per parent(m)and inbreeding coefficient(IC)of the initial lines(F_(L)).A formula for the IC of the Syn_(TC)was derived that,given any values of F_(L)(0≤F_(L)≤1)and t,is specific for any value of m,not just for“large”numbers associated with the context in which the Hardy-Weinberg law is *** was found that F_(m)^(f)Syn_(TC)is very sensitive to changes in m when m is not greater than eight,after which it tends to stabilize very *** summary,unlike previously derived formulas,F_(m)^(f)Syn_(TC)is exact for any values of t,m and F_(L).

关键词： Genotypic array genotypic mean hybrid random mating Zea mays L

Association of NOS3and HIF1αgene polymorphisms with the susceptibility of broiler chickens to develop hypoxic pulmonary hypertension

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Agricultural Sciences 2013年第12期4卷 749-755页

作者： Juana Moncaleano-Vega Fernando Ariza aureliano Hernández Department of Production Animal Universidad Nacional de Colombia Bogotá Colombia Laboratory of Animal Cytogenetics and Molecular Genetics Faculty of Veterinary Medicine and Zootecnia Universidad Nacional de Colombia Bogotá Colombia Laboratory of Morphophysiology Faculty of Veterinary Medicine and Zootecnia Universidad Nacional de Colombia Bogotá Colombia

A genetic association between single nucleotide polymorphisms (SNPs) and pulmonary hypertension syndrome (PHS) was established in a commercial population of broiler chickens. The associated SNPs were found in the NOS3 and HIF1α genes (LOD > 6;p NOS3 gene interfere with its trans-activation and transcriptional activation activities under natural hypobaric hypoxia conditions and are located in a consensus sequence that is called the hypoxia response element (HRE). SNPs located in the HIF1α gene could act as alternative cryptic splicing sites in intron six, which may stimulate non-sense mediated early decay (NMD) of the primary transcript. A fragment of intron 3 of the EDN1 gene was also evaluated, but the polymorphisms found were not associated with PHS (lod 0.001). However, further studies on the regulatory transcription sequences of EDN1 are recommended. The findings of this study indicate that intronic sequences should be included when searching for polymorphisms that produce physiological changes. Introns have transcriptional regulatory sequences or post-transcriptional control signals, which are known as cis- and trans-activation regulatory elements and are able to alter the physiological processes of hypoxia adaptation when modified. Based on these findings, it can be concluded that the inheritance pattern of PHS is autosomal overdominant and has deleterious effects that are characterized by higher penetrance in heterozygous than in homozygous animals, which prevent broiler chickens from being able to adapt to high altitudes.

关键词： Cis and Trans-Activation Regulatory Elements Deleterious Effect Penetrance

Atypical Cutaneous Leishmaniasis by Leishmania Mexicana: A Case Report with Dermoscopic, Histopathological and Molecular Study

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Advances in Microbiology 2022年第11期12卷 597-602页

作者： Marina Romero-Navarrete Lucio Vera-Cabrera Roberto Arenas María Elisa Vega-Memije aureliano Castillo-Solana General Hospital of Acapulco Secretary of Health Guerrero Mexico Dr. José Eleuterio Gonzalez University Hospital University of Nuevo Leon Monterrey Mexico Dr. Manuel Gea Gonzalez General Hospital Mexico City Mexico

Cutaneous leishmaniasis is a neglected tropical skin endemic disease, with worldwide distribution. By 2020 in the Americas, Mexico was in 12th place with 11 states reporting new cases. Molecular biology with different targets for diagnosis and species identification has been used for decades, also dermoscopy, a non-invasive diagnostic tool has shown its usefulness. We present the first case of cutaneous leishmaniasis in non-endemic place (Guerrero, Mexico) identifying Leishmania mexicana with molecular biology, and treated with itraconazole.

关键词： Atypical Cutaneous Leishmaniasis Neglected Tropical Skin Diseases Lutzomyia Leishmania mexicana Itraconazole

Familial White Piedra Caused by Trichosporon inkin

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Advances in Microbiology 2022年第7期12卷 379-385页

作者： Marina Romero-Navarrete Francisca Hernández-Hernández Roberto Arenas aureliano Castillo-Solana Brianda Stephanie Herrera-Ramírez Erika Córdova-Martínez General Hospital of Acapulco Secretary of Health Guerrero Mexico Mycology Unit Department of Microbiology and Parasitology School of Medicine National Autonomous University of Mexico Mexico City Mexico “Dr. Manuel Gea Gonzalez” General Hospital Mexico City Mexico

White piedra is a superficial, chronic, asymptomatic mycoses caused by yeast fungi of the genus Trichosporon. It affects the hair, especially of the head, less frequently of the pubis, perineum, armpit, beard, mustache, eyebrows and eyelashes, and is characterized by the formation of soft nodules or fungal clusters. It affects all age groups and both sexes, predominantly women. Diagnosis is made by direct examination of the affected hair and culture on Sabouraud dextrose agar. The identification of species occurs through more specific identification procedures, such as mass spectrometry (MALDI-TOF) and PCR). The objective of this work is to report two cases of familial white piedra caused by T. inkin identified by PCR.

关键词： Familial White Piedra Trichosporon inkin Trichosporon sp. Superficial Mycosis Polymerase Chain Reaction (PCR)

Water Quality Assessment of a Trout Farming Effluent,Bocaina,Brazil

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Journal of Water Resource and Protection 2014年第10期6卷 909-915页

作者： Bernardo Pinto Caramel Munique de Almeida Bispo Moraes Clovis Ferreira do Carmo Andre Martins Vaz-dos-Santos Yara Aiko Tabata Joao Alexandre Saviolo Osti Carlos Massatoshi Ishikawa Marcos aureliano Silva Cerqueira Cacilda Thais Janson Mercante Water Resources Center Fisheries InstituteSao PauloBrazil Federal University of Parana CuritibaBrazil Experimental Station of Salmon Sao PauloBrazil

The concern in mitigating the negative impact generated by the discharge of nutrients in the receiving water body is a challenge for the sustainable development of Brazilian fish farms. Thus, the purpose of this study was to evaluate the water quality and environmental impact caused by trout farming system effluent with focus on discharge of phosphorus. Sampling was performed on a weekly basis in triplicate from September to November 2010. Sample sites were distributed according to the water flow: upstream from trout farming system, water supply, effluent, artificial wetland, mixing zone and downstream (60 m from effluent). In the field, pH, conductivity, dissolved oxygen, water temperature and turbidity were measured. In laboratory, nitrogen and phosphorus series, chlorophyll a, total solids suspended and their organic and inorganic fractions were analyzed. For the good growth of trout in production system, the abiotic factors described in the water quality monitoring demonstrated acceptable values. Environmentally, after passing through the production system and artificial wetland, there was an increase in concentrations of total phosphorus, total nitrogen, orthophosphate, ammonium, chlorophyll a, total solids suspended and their organic and inorganic fractions (P < 0.05). These results are related with the quality of feed, feeding management and the inefficiency of the artificial wetland. Therefore, it is necessary to use best quality feed to meet the nutritional requirements of trout, maintaining an optimal feed conversion and reducing pollution generated by effluent.

关键词： Management Feeding Water Flow Discharge Phosphorus Pollution