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Immune regulatory networks coordinated by glycans and glycan-binding proteins in autoimmunity and infection

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Cellular & Molecular Immunology 2023年第10期20卷 1101-1113页

作者： SaloméS.pinho Inês alves Joana Gaifem Gabriel A.Rabinovich i3S–Institute for Research and Innovation in Health University of Porto4200-135PortoPortugal ICBAS-School of Medicine and Biomedical Sciences University of Porto4050-313PortoPortugal Faculty of Medicine University of Porto4200-319PortoPortugal Laboratorio de Glicomedicina Instituto de Biología y Medicina Experimental(IBYME)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)C1428Ciudad de Buenos AiresArgentina Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresC1428Ciudad de Buenos AiresArgentina

The immune system is coordinated by an intricate network of stimulatory and inhibitory circuits that regulate host responses against endogenous and exogenous *** of these safeguard and homeostatic mechanisms can lead to unpredictable inflammatory and autoimmune responses,whereas deficiency of immune stimulatory pathways may orchestrate immunosuppressive programs that contribute to perpetuate chronic infections,but also influence cancer development and *** have emerged as essential components of homeostatic circuits,acting as fine-tuners of immunological responses and potential molecular targets for manipulation of immune tolerance and activation in a wide range of pathologic *** surface glycans,present in cells,tissues and the extracellular matrix,have been proposed to serve as“self-associated molecular patterns”that store structurally relevant biological *** responsibility of deciphering this information relies on different families of glycan-binding proteins(including galectins,siglecs and C-type lectins)which,upon recognition of specific carbohydrate structures,can recalibrate the magnitude,nature and fate of immune *** process is tightly regulated by the diversity of glycan structures and the establishment of multivalent interactions on cell surface receptors and the extracellular *** we review the spatiotemporal regulation of selected glycan-modifying processes including mannosylation,complex N-glycan branching,core 2 O-glycan elongation,LacNAc extension,as well as terminal sialylation and ***,we illustrate examples that highlight the contribution of these processes to the control of immune responses and their integration with canonical tolerogenic ***,we discuss the power of glycans and glycan-binding proteins as a source of immunomodulatory signals that could be leveraged for the treatment of autoimmune inflammation and chronic infection.

关键词： Immune response Inflammation Infection Glycans Glycosylation Glycan-binding proteins

Mannosylated glycans impair normal T-cell development by reprogramming commitment and repertoire diversity

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Cellular & Molecular Immunology 2023年第8期20卷 955-968页

作者： Manuel M.Vicente Inês alves Ângela Fernandes Ana M.Dias Beatriz Santos-Pereira Elena Pérez-Anton Sofia Santos Tao Yang Alexandra Correia Anja Münster-Kühnel Afonso R.M.Almeida Sarina Ravens Gabriel A.Rabinovich Manuel Vilanova Ana E.Sousa SaloméS.pinho i3S–Institute for Research and Innovation in Health University of PortoPortoPortugal Institute of Biomedical Sciences Abel Salazar(ICBAS) University of PortoPortoPortugal Graduate Program in Areas of Basic and Applied Biology(GABBA) ICBASUniversity of PortoPortoPortugal Faculty of Medicine University of PortoPortoPortugal Nephrology Department Centro Hospitalar e Universitário do PortoPortoPortugal Institute of Immunology Hannover Medical SchoolHannoverGermany Institute of Clinical Biochemistry Hannover Medical SchoolHannoverGermany Instituto de Medicina Molecular João Lobo Antunes Faculdade de MedicinaUniversidade de LisboaLisboaPortugal Laboratorio de Inmunopatología Instituto de Biología y Medicina Experimental(IBYME)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Ciudad de Buenos AiresArgentina Laboratorio de Inmuno-oncología Translacional Instituto de Biología y Medicina Experimental(IBYME)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Ciudad de Buenos AiresArgentina Facultad de Ciencias Exactas y Naturales(FCEyN) Universidad de Buenos AiresCiudad de Buenos AiresArgentina

T-cell development ensures the formation of diverse repertoires of T-cell receptors(TCRs)that recognize a variety of *** is a major posttranslational modification present in virtually all cells,including T-lymphocytes,that regulates activity/*** these structures are known to be involved in TCR-selection in DP thymocytes,it is unclear how glycans regulate other thymic development processes and how they influence susceptibility to ***,we discovered stage-specific glycome compositions during T-cell development in human and murine thymocytes,as well as dynamic *** restricting the N-glycosylation profile of thymocytes to high-mannose structures,using specific glycoengineered mice(Rag1CreMgat1fl/fl),we showed remarkable defects in key developmental checkpoints,includingß-selection,regulatory T-cell generation andγδT-cell development,associated with increased susceptibility to colon and kidney inflammation and *** further demonstrated that a single N-glycan antenna(modeled in Rag1CreMgat2fl/fl mice)is the sine-qua-non condition to ensure normal *** conclusion,we revealed that mannosylated thymocytes lead to a dysregulation in T-cell development that is associated with inflammation susceptibility.

关键词： N-glycosylation T-cell development Thymocytes Glycocalyx Inflammation

Physiological and Biochemical Characterization of JilóSeeds (Solanum gilo) in Different Harvest Times

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American Journal of Plant Sciences 2017年第10期8卷 2569-2595页

作者： Marcus Vinicius Prado alves Edila Vilela De Resende Von pinho Heloisa Oliveira Dos Santos Gustavo Costa Prado alves Rucyan Walace Pereira Agriculture Department Federal University of Lavras Lavras Brazil

The evaluation of seed quality during the development process is important for the definition of the moment of harvest. In this context, the objective in this study was to evaluate the quality of Jiló seeds during development through physiological, biochemical and electrophoretic tests of enzymes. Six genetic materials, JIL white, JIL 001, JIL 005, JIL 006, H1 and H2 were used. Fruits were harvested at 35, 40, 45 days after anthesis (DAA). A total of 50% of all fruits harvested at 45 DAA remained at rest for seven days which were labeled as 45 DAAr. The seeds at each maturation stage were extracted from the fruits and evaluated by water content, weight of one thousand seeds, electric conductivity, germination, germination speed index, seedling emergence, and seedling emergence rate index. The chemical composition of the seeds was also evaluated, as well as the expression of the enzymes esterase, isocitrate lyase, malate dehydrogenase, alcohol dehydrogenase, superoxide dismutase, catalase, peroxidase and endo-β-mannanase. The highest values of germination and vigor were found in seeds harvested at 45 DAA and 45 DAAr, mainly for hybrids H1 and H2. The highest values of lipids and proteins and lower carbohydrates were found in the most immature seeds of Jiló. The enzymes analyzed showed higher expression in the initial stages of development, however reduced with the advancement of the maturation stage. Endo-β-mannanase expression increased with seed development. Jiló seeds harvested at 45 DAAr presented the highest of germination and vigor values. This “being” considered as the ideal harvest point.

关键词： Seed Quality Germination Enzyme Harvesting Point Seed Development

Fermentative and Bromatological Characteristics of Piata Palisadegrass Ensiled with Energetic Brans

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American Journal of Plant Sciences 2014年第7期5卷 942-954页

作者： Rozana Castro Perim Kátia Aparecida de pinho Costa Patrícia Soares Epifanio Wender Ferreira de Souza Ricardo Francischini Daniel augusto alves Teixeira Walkíria Guimar?es Carvalho Divino Rosa dos Santos Jr. Goiano Federal Institute Rio VerdeBrazil Federal University of Bahia SalvadorBrazil University of Rio Verde Rio VerdeBrazil

The objectives of this study were to evaluate the fermentative and bromatological characteristics of Piata palisadegrass that was ensiled with energetic brans and to assess the costs of different additive levels. This experiment was conducted at the Goiano Federal Institute on the Rio Verde campus in Goiás State, Brazil. The experimental design was entirely random with four replications in a 4 × 5 factorial scheme that included four energetic brans (millet, maize, sorghum and wheat) and five levels (0%, 8%, 16%, 24% and 32%). The energetic brans can be considered as a good source of additives for Piata palisadegrass ensiling, which improves the qualitative and nutritional fermentative characteristics of the silage. The maize and wheat brans were the most efficient for improving the fermentative characteristics of the silage. However, millet bran was the most efficient for improving the bromatological characteristics. In addition, the wheat bran had the greatest influence on the final production cost and the sorghum and millet brans were the most economically feasible. Overall, the addition of 24% bran is recommended due to its beneficial impact on silage quality and the average final production cost of the silage.

关键词： Organic Acids Brachiaria brizantha Ensiling Nutritive Value

Recent Extremes of Drought and Flooding in Amazonia: Vulnerabilities and Human Adaptation

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American Journal of Climate Change 2013年第2期2卷 87-96页

作者： Jose A. Marengo Laura S. Borma Daniel A. Rodriguez Patrícia pinho Wagner R. Soares Lincoln M. alves Earth System Science Center CCST/National Institute for Space Research INPE Sao Paulo Brazil

The present study focuses on the impacts of extreme drought and flooding situations in Amazonia, using level/discharge data from some rivers in the Amazon region as indicators of impacts. The last 10 years have featured various “once in a century” droughts and floods in the Amazon basin, which have affected human and natural systems in the region. We assess a history of such hazards based on river data, and discuss some of the observed impacts in terms of vulnerability of human and natural systems, as well as some of adaptation strategies implemented by regional and local governments to cope with them. A critical perspective of mitigation of drought and flood policies in Amazonia suggests that they have been mostly ineffective in reducing vulnerability for the majority of the population, constituting, perhaps, examples of maladaptation via the undermining of resilience.

关键词： Climate Variability River Level Vulnerability Amazonia Extremes Hazards

Nicotinic cholinergic receptors in esophagus:Early alteration during carcinogenesis and prognostic value

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World Journal of Gastroenterology 2016年第31期22卷 7146-7156页

作者： Marina Chianello Nicolau Luis Felipe Ribeiro Pinto Pedro Nicolau-Neto Paulo Roberto alves de pinho Ana Rossini Tatiana de Almeida Simao Sheila Coelho Soares Lima Departamento de Bioquímica Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de Janeiro Programa de Carcinogênese Molecular Instituto Nacional de CancerCoordenacao de Pesquisa Gastroenterologia Hospital Universitário Pedro ErnestoUniversidade do Estado do Rio de Janeiro

AIM: To compare expression of nicotinic cholinergic receptors(CHRNs) in healthy and squamous cell carcinoma-affected esophagus and determine the prognostic ***: We performed RT-q PCR to measure the expression of CHRNs in 44 esophageal samples from healthy individuals and in matched normal surrounding mucosa, and in tumors from 28 patientsdiagnosed with esophageal squamous cell carcinoma(ESCC). Next, we performed correlation analysis for the detected expression of these receptors with the habits and clinico-pathological characteristics of all study participants. In order to investigate the possible correlations between the expression of the different CHRN subunits in both healthy esophagus and tissues from ESCC patients, correlation matrices were generated. Subsequently, we evaluated whether the detected alterations in expression of the various CHRNs could precede histopathological modifications during the esophageal carcinogenic processes by using receiver operating characteristic curve analysis. Finally, we evaluated the impact of CHRNA5 and CHRNA7 expression on overall survival by using multivariate ***: CHRNA3, CHRNA5, CHRNA7 and CHRNB4, but not CHRNA1, CHRNA4, CHRNA9 or CHRNA10, were found to be expressed in normal(healthy) esophageal mucosa. In ESCC, CHRNA5 and CHRNA7 were overexpressed as compared with patient-matched surrounding non-tumor mucosa(ESCC-adjacent mucosa; P < 0.0001 and P = 0.0091, respectively). Positive correlations were observed between CHRNA3 and CHRNB4 expression in all samples analyzed. Additionally, CHRNB4 was found to be differentially expressed in the healthy esophagus and the normalappearing ESCC-adjacent mucosa, allowing for distinguishment between these tissues with a sensitivity of 75.86% and a specificity of 78.95%(P = 0.0002). Finally, CHRNA5 expression was identified as an independent prognostic factor in ESCC; patients with high CHRNA5 expression showed an increased overall survival, in comparison with those

关键词： Nicotinic cholinergic receptors Esophagus Esophageal squamous cell carcinoma Tobacco Alcohol Gene expression

Yield and Chemical Composition of Brachiaria Forage Grasses in the Offseason after Corn Harvest

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American Journal of Plant Sciences 2014年第7期5卷 933-941页

作者： Gean alves Maia Kátia Aparecida de pinho Costa Eduardo da Costa Severiano Patrícia Soares Epifanio José Flávio Neto Matheus Goncalves Ribeiro Patrick Bezerra Fernandes José Fausto Guimaraes Silva Wainer Gomes Goncalves Laboratory of Soil Physics Federal Institute GoianoRio VerdeBrazil Laboratory of Forage and Grassland Federal Institute GoianoRio VerdeBrazil

This study evaluated the dry matter production and chemical composition of Brachiaria forage grasses in the offseason after corn harvest in integrated crop-livestock system. The experiment was conducted at the Federal Institute of Goiás, Rio Verde Campus, using a randomized complete block experimental design, with four replications. The treatments consisted of the forages: Brachiaria brizantha cv. Marandu;Brachiaria brizantha cv. Xaraes;Brachiaria brizantha cv. Piata;Brachiaria brizantha cv. MG-4;Brachiaria decumbens and Brachiaria ruziziensis, intercropped in oversown corn for implantation of integrated crop-livestock system. The results showed that intercropping corn with Brachiaria grasses favors the production of high-quality forage in the offseason, and the cultivars of Brachiaria brizantha and Brachiaria decumbens showed higher dry matter production. And cultivars of Brachiaria brizantha (Marandu palisadegrass, Xaraes palisadegrass and Piata palisadegrass) are the most suitable for presenting food of better quality, compared with Brachiaria brizantha cv. MG-4, Brachiaria decumbens and Brachiaria ruziziensis.

关键词： Brachiaria brizantha Brachiaria decumbens Brachiaria ruziziensis Crop-Livestock Integration

SARS-CoV-2 and hearing:An audiometric analysis of COVID-19 hospitalized patients

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Journal of Otology 2021年第3期16卷 158-164页

作者： Francisco alves de Sousa Rodrigo Pinto Costa Sandra Xará Ana Nóbrega Pinto Cecília Almeida e Sousa Centro Hospitalar Universitario do Porto Serviço de Otorrinolaringologia e Cirurgia da Cabeça e Pescoço(Otorhinolaryngology and Head&Neck surgery Department)Largo Prof.Abel Salazar4099-001PortoPortugal Unidade de Saúde Familiar Garcia de Orta ACeS Porto OcidentalMedicina Geral e Familiar(Family Medicine)R.Pinho Leal 294150-620PortoPortugal Centro Hospitalar Universitario do Porto Serviço de Doenças Infeciosas(Infectious Diseases Department)Largo Prof.Abel Salazar4099-001PortoPortugal

Purpose COVID-19 associated hearing loss is still an ongoing matter of *** original studies exist on audiological effects of SARS-CoV-2 infection in hospitalized *** main objective was to determine whether SARS-CoV-2 may affect auditory function in clinically ill COVID-19 *** and methods COVID-19 patients with moderate-severe disease and without prior history of hearing abnormalities were enrolled from a tertiary referral center,and matched with *** performed an audiometric evaluation,and thresholds were *** 120 ears from 60 patients were *** with COVID-19 showed worse mean auditory thresholds starting from 1000 Hz through higher frequencies,when compared to controls(1000 Hz:18.52±5.49 dB HL in controls vs 25.36±6.79 dB HL in COVID-19,p<0.001;2000Hz:17.50±5.57 dB HL in controls vs 21.96±7.05 dB HL in COVID-19,p=0.010;3000Hz:17.97±8.07 dB HL in controls vs 25±9.38 dB HL in COVID-19,p=0.003;4000 Hz:20.16±10.12 dB HL in controls vs 29.55±11.26 dB HL in COVID-19,p=0.001;8000 Hz:31.09±12.75 dB HL in controls vs 40.71±19.40 dB HL in COVID-19,p=0.030;Pure Tone Average:20.42±4.29 dB HL in controls vs 24.85±5.62 dB HL in COVID-19,p=0.001).Statistical significance persisted after adjusting for confounders such as age,gender and various comorbidities(p<0.05).Conclusions SARS-CoV-2 may affect hearing in COVID-19 patients with moderate-severe *** are in line with the previous suggested effects of COVID-19 on auditory *** study is expected to encourage further research on this topic.

关键词： SARS-CoV-2 COVID-19 Coronavirus Audiometry Hearing

Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?

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World Journal of Gastroenterology 2013年第42期19卷 7399-7404页

作者： Raquel Francine Liermann Garcia Simone Moreira Ana Lucia de Araújo Ramos Leslie Ecker Ferreira Angelo alves de Mattos Cristiane Valle Tovo Lysandro Alsina Nader Juliene Antonio Ramos Edson Rondinelli Arnaldo de Jesus Dominici Christian Evangelista Garcia Mauro de Souza Leite pinho Carlos Eduardo Brando-Mello Cristiane alves Villela-Nogueira Paulo Henrique Condeixa de Frana Departamento de Medicina Universidade da Regio de Joinville/UnivilleJoinville89219-719 Santa CatarinaBrazil Departamento de Clinica Médica e Cirurgia Geral Hospital Municipal So José Joinville89202-000 Santa CatarinaBrazil Departamento de Clínica Médica Servio de HepatologiaFaculdade de Medicina/Hospital Universitário Clementino Fraga Filho/Universidade Federal do Rio de Janeiro21941-913 Rio de JaneiroBrazil Servio de Gastroenterologia e Hepatologia Irmandade da Santa Casa de Misericórdia/ISCMPAPorto Alegre90020-090 Rio Grande do SulBrazil Servio de Endoscopia Universidade Federal de Pelotas/UFPelPelotas96010-610 Rio Grande do SulBrazil Instituto de Biofísica Carlos Chagas Filho/UFRJ 21941-902 Rio de JaneiroBrazil Departamento de Gastroenterologia Hospital Universitário da Universidade Federal do Maranh o/UFMASo Luis65020-560 MaranhoBrazil Servio de Gastroenterologia Hospital Universitário Gaffrée Guinle/HUGG/UNIRIO20270-004 Rio de JaneiroBrazil

AIM:To analyze the role of rs12979860 and rs8099917polymorphisms in hepatitis C virus(HCV)genotype 1infection of ***:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C(CHC)who had completed a 48-wk regimen of pegylated-interferonα-2a or-2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and199 healthy blood donors(controls)from a single site between January 2010 and January *** on the patients’response to treatment was *** chain reaction-restriction fragment length polymorphism genotyping of the interleukin(IL)28B gene fragment encompassing the single nucleotide polymorphisms(SNPs)rs12979860(C/T)and rs8099917(T/G)was carried out for 79 of the CHC patients and 199 of the ***-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC ***:SNP rs12979860 genotyping was successful in 99.5%of the controls and 97.2%of the CHC patients,whereas the SNP rs8099917 genotyping was successful in 95.5%of the controls and 100%of the CHC *** genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups,with significantly higher genotype frequencies of CC and TT in the controls(P=0.037 and 0.046,respectively)and of TT and GG in the CHC patients(P=0.0009and 0.0001,respectively).Analysis of the CHC patients who achieved sustained virological response(SVR)to treatment(n=55)indicated that the rs12979860 C allele and CC genotype were predictors of SVR(P=0.02).No significant correlation was found between rs8099917 genotypes and treatment response,but carriers of the T allele showed significantly higher rates of SVR(P=0.02).Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917(P=0.07).CONCLUSION:The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCVinfec

关键词： Hepatitis C Interleukin 28B Single nucleotide polymorphisms Sustained virological response Brazil