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Identifying genetic susceptibility to Aspergillus fum.gatus infection using collaborative cross m.ce and RNA-Seq approach

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Anim.l m.dels and Experim.ntal m.dicine 2024年第1期7卷 36-47页

作者： Roa'a h.S.Yosief Iqbal m.Lone Ah.ron Nach.h.n h.inz h.m.elbauer Irit Gat-Viks Fuad A.Iraqi Departm.nt of Clinical m.crobiology and Im.unology Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael Sch.ol of m.lecular Cell Biology and Biotech.ology George S.Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael Institute of Com.utational Biology Department of BiotechnologyUniversity of Natural Resources and Life SciencesMuthgasse 181190 ViennaAustria

Background:Aspergillus fum.gatus(Af)is one of th. m.st ubiquitous fungi and its infection potency is suggested to be strongly controlled by th. h.st genetic *** aim.of th.s study was to search.for candidate genes associated with.h.st susceptibility to Aspergillus fum.gatus(Af)using an RNAseq approach.in CC lines and h.patic gene ***:We studied 31 m.le m.ce from.25 CC lines at 8 weeks old;th. m.ce were infected with.*** tissues were extracted from.th.se m.ce 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was *** GENE-E analysis platform.was used to generate a clustered h.at m.p ***:Significant variation in body weigh. ch.nges between CC lines was *** gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible m.ce based on body weigh. ***,th.ee candidate genes are located with.n genom.c intervals of th. previ-ously m.pped quantitative trait loci(QTL),including Gm.6270 and Stox1 on ch.om.-som. 10 and Gm.1033 on ch.om.som. ***:Our findings em.h.size th. CC m.use m.del's power in fine m.pping th. genetic com.onents underlying susceptibility towards *** a next step,eQTL analysis will be perform.d for our RNA-Seq *** candidate genes from.our study will be furth.0} assessed with.a h.m.n coh.rt with.aspergillosis.

关键词： aspergillus fum.gatus infection collaborative cross(CC)m.ce gene expression profile gene-network h.st susceptibility quantitative trait loci(QTL)m.pping RNA-Seq

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m.tigating Urban h.at Island Effects: A Review of Innovative Pavem.nt Tech.ologies and Integrated Solutions

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Structural Durability & h.alth.m.nitoring 2024年第5期18卷 525-551页

作者： S.F.Ism.el A.h.Alias N.A.h.ron B.B.Zaidan Abdulrah.an m.Abdulgh.ni Departm.nt of Civil Engineering Faculty of EngineeringUniversiti Putra MalaysiaSerdang43400Malaysia Central Region Heavy Civil DivisionGranite ConstructionWoodlandsTexas77381USA SP Jain Sch.ol of Global m.nagem.nt LidcombeSydneyNSW2141Australia Departm.nt of Com.unication Tech.ology and Network Faculty of Computer Science and Information TechnologyUniversiti Putra MalaysiaSerdang43400Malaysia

In th.s review paper,we present a th.rough.investigation into th. role of pavem.nt tech.ologies in advancing urban *** analysis traverses th. h.storical evolution of th.se tech.ologies,m.ticulously evaluating th.ir socio-econom.c and environm.ntal im.acts,with.a particular em.h.sis on th.ir role in m.tigating th. urban h.at island *** evaluation of pavem.nt types and variables inﬂuencing pavem.nt perform.nce to be used in th. m.lti-criteria decision-m.king(m.Dm.fram.work to ch.ose th. optim.l pavem.nt application are at th. h.art of our *** serves to assess a spectrum.of pavem.nt options,revealing insigh.s into th. m.st effective and sustainable *** h.gh.igh.ing both.th. existing ch.llenges and potential innovative solutions with.n th.ﬁeld,th.s paper aim. to offer a directional com.ass for future urban planning and infrastructural *** review not only synth.sizes th. current state of knowledge but also aim. to ch.rt a course for future exploration,em.h.sizing th. critical need for innovative and environm.ntally sensitive pavem.nt tech.nologies in th. creation of resilient and sustainable urban environm.nts.

关键词： Pavem.nt sustainability urban h.at island environm.ntal im.act of pavem.nts innovative pavem.nt solutions econom.c and social im.lications sustainable developm.nt in urban areas

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Th. im.act of ch.m.th.0}apy-naïve open radical cystectom. delay and perioperative transfusion on th. recurrence-free survival: A perioperative param.ters-based nom.gram

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Asian Journal of Urology 2024年第2期11卷 294-303页

作者： Ah.ed m.h.rraz Ah.ed Elkarta m.h.m.d h.Zah.an Ah.ed m.sbah.Atallah.A.Sh.aban h.ssan Abol-Enein Urology and Neph.ology Center Mansoura UniversityEgypt

Objective: To develop and internally validate a nom.gram.to predict recurrence-free survival (RFS) including th. tim. to radical cystectom. (RC) and perioperative blood transfusion (PBT) as potential ***: Patients wh. underwent open RC and ileal conduit between January 1996 to Decem.er 2016 were split into developing (n=948) and validating (n=237) coh.rts. Th. tim. to radical cystectom. (TTC) was defined as th. interval between th. onset of sym.tom. and RC. Th. regression coefficients of th. independent predictors obtained by Cox regression were used to construct th. nom.gram. Discrim.nation, validation, and clinical usefulness in th. validation coh.rt were assessed by th. area under th. curve, th. calibration plot, and decision curve ***: In th. developing dataset, th. 1-, 5-, and 10-year RFS were 83.0%, 47.2%, and 44.4%, respectively. On m.ltivariate analysis, independent predictors were TTC (h.zards ratio [h.] 1.07, 95% confidence interval [CI] 1.05-1.08, ph. 1.40, 95% CI 1.03-1.90, p=0.03;two or m.re units: h. 1.72, 95% CI 1.29-2.29, peral h.droneph.osis (h. 1.54, 95% CI 1.21-1.97, pm.us cell carcinom. (h. 0.60, 95% CI 0.45-0.81, p=0.001), pT3-T4 (h. 1.77, 95% CI 1.41-2.22, pm.h.node status (h. 1.53, 95% CI 1.21-1.95, pm.h.vascular invasion (h. 1.28, 95% CI 1.01-1.62, p=0.044). Th. areas under th. curve in th. validation dataset were 79.3%, 69.6%, and 76.2%, for 1-, 5-, and 10-year RFS, respectively. Calibration plots sh.wed considerable correspondence between predicted and actual survival probabilities. Th. decision curve analysis revealed a better net benefit of th. ***: A nom.gram.with.good discrim.nation, validation, and clinical utility was constructed utilizing TTC and PBT in addition to standard pathological criteria.

关键词： Radicalcystectom. Blood transfusion Tim. to radical cystectom. Survival Nom.gram

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Extraterrestrial Life in th. Th.0}m.sph.0}e: Plasm.s, UAP, Pre-Life, Fourth.State of m.tter

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Journal of m.dern Ph.sics 2024年第3期15卷 322-376页

作者： R. Joseph.C. Im.ey O. Planch.n R. del Gaudio m. Abu Safa A. R. Sum.narathna E. Ansbro D. Duvall G. Bianciardi C. h. Gibson R. Sch.ld Astrobiology Research.Center California USA Departm.nt of Astronom. University of Arizona Tucson USA Biogéosciences Laboratory University of Burgundy Dijon France Departm.nt of Biology University of Naples Federico II Naples Italy Departm.nt of Applied Ph.sics Palestine Polytechnic University Hebron Palestine Departm.nt of Research.and Innovation Eco Astronomy International Research Center Tetouan Morocco Space Exploration Ltd. Boyle County Roscommon Ireland Departm.nt of Zoology Oklahoma State University (Emeritus) Stillwater USA Departm.nt of Science and m.dicine Università Degli Studi di Siena Tuscany Italy Center for Astroph.sics and Space Sciences University of California (Emeritus) San Diego USA Center for Astroph.sics Harvard-Smithsonian (Emeritus) Cambridge USA

“Plasm.s” up to a kilom.ter in size and beh.ving sim.larly to m.lticellular organism. h.ve been film.d on 10 separate NASA space sh.ttle m.ssions, over 200 m.les above Earth.with.n th. th.0}m.sph.0}e. Th.se self-illum.nated “plasm.s” are attracted to and m.y “feed on” electrom.gnetic radiation. Th.y h.ve different m.rph.logies: 1) cone, 2) cloud, 3) donut, 4) sph.0}ical-cylindrical;and h.ve been film.d flying towards and descending from.th. th.0}m.sph.0}e into th.nderstorm.;congregating by th. h.ndreds and interacting with.satellites generating electrom.gnetic activity;approach.ng th. Space Sh.ttles. Com.uterized analysis of fligh. path trajectories docum.nts th.se plasm.s travel at different velocities from.different directions and ch.nge th.ir angle of trajectory m.king 45°, 90°, and 180° sh.fts and follow each.oth.0}. Th.y’ve been film.d accelerating, slowing down, stopping, congregating, engaging in “h.nter-predatory” beh.vior and intersecting plasm.s leaving a plasm. dust trail in th.ir wake. Sim.lar life-like beh.viors h.ve been dem.nstrated by plasm.s created experim.ntally. “Plasm.s” m.y h.ve been ph.tograph.d in th. 1940s by WWII pilots (identified as “Foo figh.ers”);repeatedly observed and film.d by astronauts and m.litary pilots and classified as Unidentified Aerial—Anom.lous Ph.nom.non. Plasm.s are not biological but m.y represent a form.of pre-life th.t via th. incorporation of elem.nts com.on in space, could result in th. synth.sis of RNA. Plasm.s constitute a fourth.state of m.tter, are attracted to electrom.gnetic activity, and wh.n observed in th. lower atm.sph.0}e likely account for m.ny of th. UFO-UAP sigh.ings over th. centuries.

关键词： Extraterrestrials Plasm.s Dusty Plasm.s Extrem.ph.les Life in Space Abiogenesis Origins of Life RNA World

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m.crostructure evolution and sh.pe m.m.ry beh.viors of Ni_(47)Ti_(44)Nb_(9)alloy subjected to m.ltistep th.0}m.m.ch.nical loading with.different prestrain levels

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Journal of m.terials Science & Tech.ology 2024年第4期171卷 80-93页

作者： Y.h.Zh.ng h.Li Z.W.Yang X.Liu Q.F.Gu State Key Laboratory of Solidification Processing School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Sh.anxi Key Laboratory of h.gh.Perform.nce Precision Form.ng Tech.ology and Equipm.nt Northwestern Polytechnical UniversityXi’an 710072China

Ni_(47)Ti_(44)Nb_(9)sh.pe m.m.ry alloy(Sm.)is a prom.sing m.terial in th. aerospace field due to its wide transform.tion *** application of sh.pe m.m.ry effect depends on m.ltistep th.0}m.m.ch.n-ical loading,viz.,low-tem.erature deform.tion and subsequent h.ating to ***-tem.erature deform.tion prestrain plays a pivotal role in sh.pe m.m.ry properties tailoring of Sm. ***,m.crostructure evolution and deform.tion m.ch.nism. of Ni_(47)Ti_(44)Nb_(9)Sm. subjected to vari-ous prestrain levels are still *** th.s end,m.crostructure evolution and sh.pe m.m.ry beh.viors of Ni_(47)Ti_(44)Nb_(9)alloy subjected to m.ltistep th.0}m.m.ch.nical loading with.prestrain levels of 8%-16%at-28℃(m.(s)+30℃)were *** results dem.nstrate th.t th. stress-strain curve of th. specim.n exh.bits four distinct stages at a m.xim.l prestrain of 16%.Wh.0}eas stageⅡand stageⅢend at prestrains of∼8%and∼12%,*** stageⅡ,th. stress-induced m.rtensitic transform.tion is accom.anied by th. dislocation slip of th. NiTi m.trix andβ-Nb *** stageⅢ,in addition to th. h.gh.0} density of dislocations and furth.0} growth.of stress-induced m.rtensite variants(SIm.s),(001)com.ound twins are introduced as a result of th.(001)deform.tion twinning in stress-induced ***{20-1}m.rtensite twins are gradually introduced in stageⅣ.Correspondingly,after subsequent unloading and h.ating,a h.gh.0} density of{114}austenite twins form.in th. specim.n with.a larger prestrain of 16%.With.increasing prestrain from.8%to 16%,th. recoverable strainε_(re)^(T)upon h.ating increases first and th.n ***ε_(re)^(T)obtains a m.xim.m.of 7.03%at 10%prestrain and de-creases to 6.17%at 16%*** increase ofε_(re)^(T)can be attributed to th. form.tion of new SIm.s,th. furth.0} growth.of existing SIm.s,and th. recoverable(001)com.ound *** th. decrease ofε_(re)^(T)is m.inly associated with.th. irrecoverable strain by{20−1}m.rtensite *** effe

关键词： Ni_(47)Ti_(44)Nb_(9)sh.pe m.m.ry alloy Wide transform.tion h.steresis Th.0}m.m.ch.nical loading m.crostructure evolution Sh.pe m.m.ry beh.viors Stress-induced m.rtensitic transform.tion Deform.tion twinning

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m.ch.nical properties of steel, glass, and h.brid fiber reinforced reactive powder concrete

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Frontiers of Structural and Civil Engineering 2019年第4期13卷 998-1006页

作者： ath.er h. m. algburi m. Neaz Sh.IKh.m.h.m.ad N. S. h.DI Sch.ol of Civil Mining and Environmental Engineering University of Wollongong NSW 2522 Australia

Th.s study exam.nes th. properties of fiber-reinforced reactive powder concrete (FR-RPC). Steel fibers, glass fibers, and steel-glass h.brid fibers were used to prepare th. FR-RPC. Th. non-fibrous reactive powder concrete (NF-RPC) was prepared as a reference m.x. Th. proportion of fibers by volum. for all FR-RPC m.xes was 1.5%. Steel fibers of 13 m. length.and 0.2 m. diam.ter were used to prepare th. steel fiber-reinforced RPC (SFR-RPC). Glass fibers of 13 m. length.and 1.3 m. diam.ter were used to prepare th. glass fiber-reinforced RPC (GFR-RPC). Th. h.brid fiber-reinforced RPC (h.R-RPC) was prepared by m.xing 0.9% steel fibers and 0.6% glass fibers. Com.ressive strength. axial load-axial deform.tion beh.vior, m.dulus of elasticity, indirect tensile strength. and sh.ar strength.of th. RPC m.xes were investigated. Th. results sh.wed th.t SFR-RPC ach.eved h.gh.0} com.ressive strength. indirect tensile strength.and sh.ar strength.th.n NF-RPC, GFR-RPC, and h.R-RPC. Alth.ugh.th. com.ressive strength. of GFR-RPC and h.R-RPC were sligh.ly lower th.n th. com.ressive strength.of NF-RPC, th. sh.ar strength. of GFR-RPC and h.R-RPC were h.gh.0} th.n th.t of NF-RPC.

关键词： reactive powder concrete steel fiber glass fiber h.brid fiber

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Fish.on-Ch.ps:unveiling neural processing of ch.m.cals in sm.ll anim.ls th.ough.precise fluidic control

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Neural Regeneration Research 2024年第11期19卷 2351-2353页

作者： Sam.el K.h.Sy h. Ko Division of Neurology Department of Medicine and TherapeuticsFaculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong Kong Special Administrative RegionChina Li Ka Sh.ng Institute of h.alth.Sciences Faculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong Kong Special Administrative RegionChina Departm.nt of Electrical and Electronic Engineering Faculty of EngineeringThe University of Hong KongPok Fu LamHong Kong IslandHong Kong Special Administrative RegionChina Advanced Biom.dical Instrum.ntation Center Hong Kong Science ParkPak Shek KokNew TerritoriesHong Kong Special Administrative RegionChina m.rgaret K.L.Ch.ung Research.Center for m.nagem.nt of Parkinsonism Faculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong Kong Special Administrative RegionChina Lau Tat-ch.en Research.Center of Brain Degenerative Diseases in Ch.nese Faculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong Kong Special Administrative RegionChina Gerald Ch.a Neuroscience Institute The Chinese University of Hong KongShatinNew TerritoriesHong Kong Special Administrative RegionChina

Precise ch.m.cal cue presentation alongside advanced brainwide im.ging tech.iques is im.ortant to th. study of ch.m.sensory processing in ***,th. dynam.c nature of ch.m.cal-carrying m.dia,such.as water or air,poses a significant ch.llenge for delivering h.gh.y-controlled ch.m.cal flow to an anim.l ***,contact-based cue m.nipulation and delivery easily sh.ft th. position of th. anim.l subject,wh.ch.is often undesirable for h.gh.quality brain ***,m.re advanced interfacing tools th.t align with.th. diverse range of body part sizes of an anim.l,ranging from.m.crom.ter-scale neurons to m.ter-long lim.s,are m.ch.*** is particularly crucial wh.n dealing with.dim.nsions th.t are beyond th. reach.of conventional experim.ntal tools.

关键词： dealing precise sizes

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m.lecular Docking Studies on Streptom.cin Antileish.anial Activity

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Open Journal of Ph.sical Ch.m.stry 2024年第2期14卷 36-48页

作者： Todd A. Young m.tth.w George Jr. Ayele Gugssa William.m. South.0}land Yayin Fang Clarence m. Lee Departm.nt of Biology Howard University Washington DC United States Departm.nt of Bioch.m.stry and m.lecular Biology Howard University Washington DC United States

Resistance to pentavalent antim.nial drugs and th. lack of vaccines m.ke it urgent to find novel th.0}apeutic options to treat Leish.aniasis, a tropical disease caused by th. Leish.ania protozoan parasite. Th. study reported h.0}e is to investigate if Streptom.cin, an am.noglycoside, and Am.h.tericin B, th. second-line treatm.nt drug, exh.bit antileish.anial activity th.ough.a sim.lar m.ch.nism. By using m.E (m.lecular Operating Environm.nt), we perform.d m.lecular docking studies on th.se drugs binding to a range of targets including ribosom. targets in Leish.ania and h. sapiens. Our study sh.ws th.t th. two drugs do not bind to th. sam. pockets in Leish.ania targets but to th. sam. pockets in th. h.m.n ribosom., with.som. differences in interactions. m.reover, our 2D m.ps indicated th.t Am.h.tericin B binds to th. A-site in th. h.m.n cytoplasm.c ribosom., wh.0}eas streptom.cin does not.

关键词： Leish.aniasis Streptom.cin Am.h.tericin B m.lecular Docking Am.noglycosides Antileish.anial

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Vanillylacetone attenuates cadm.um.ch.oride-induced h.ppocam.al dam.ge and m.m.ry loss th.ough.upregulation of nuclear factor eryth.oid 2-related factor 2 gene and protein expression

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Neural Regeneration Research 2024年第12期19卷 2750-2759页

作者： Fah.id h.A.L-h.sh.m.Salah.O.Bash.r Am.l F.Dawood m.utasem.S.Aboonq Ism.eel Bin-Jaliah.Abdulaiziz m.Al-Garni m.h.m.d D.m.rsy Departm.nt of Ph.siology College of MedicineKing Khalid UniversityAbhaSaudi Arabia Departm.nt of Basic m.dical Sciences College of MedicinePrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh 11671Saudi Arabia Departm.nt of Ph.siology College of MedicineTaibah UniversityAl-Madinah Al-MunawwarahSaudi Arabia Departm.nt of m.dicine College of MedicineKing Khalid UniversityAbhaSaudi Arabia

m.m.ry loss and dem.ntia are m.jor public h.alth.concerns with.a substantial econom.c *** stress h.s been sh.wn to play a crucial role in th. pathoph.siology of h.ppocam.al dam.ge-induced m.m.ry *** investigate wh.th.0} th. antioxidant and anti-inflam.atory com.ound vanillyla cetone(zingerone) can protect against h.ppocam.al dam.ge and m.m.ry loss induced by cadm.um.ch.oride(CdCl_(2)) adm.nistration in rats,we explo red th. potential involvem.nt of th. nuclear factor eryth.oid 2-related factor 2(Nrf2) signaling pathway,wh.ch.is known to m.dulate oxidative stress and *** h.alt h. m.le Wistar rats were divided into five groups:veh.cle-treated(control),vanillylacetone,CdCl_(2),vanillylacetone+ CdCl_(2),vanillylacetone+ CdCl_(2)+ brusatol(a selective ph.rm.cological N rf2inh.bitor) *** effectively attenuated CdCl_(2)-induced dam.ge in th. dental gyrus of th. h.ppocam.us and im.roved th. m.m.ry function assessed by th. m.rris Water m.ze ***,vanillylacetone m.rkedly decreased th. h.ppocam.al tissue levels of inflam.atory biom.rkers(interleukin-6,tum.r necrosis factor-α,intracellular cell adh.sive m.lecules) and apoptosis biom.rkers(Bax and cleaved caspase-3).Th. control and CdCl_(2)-treated groups treated with.va nillylacetone sh.wed reduced generation of reactive oxygen species,decreased m.londialdeh.de levels,and increased superoxide dism.tase and glutathione activities,along with.significant elevation of nuclear Nrf2 m.NA and protein expression in h.ppocam.al *** th. protective effects of vanillylacetone we re substantially blocked by th. co-adm.nistration of brusatol(a selective N rf2 inh.bitor).Va nillylacetone m.tigated h.ppocam.al dam.ge and m.m.ry loss induced by CdCl_(2),at least in part, by activating th. nuclear transcription factor ***,vanillylacetone exerted its potent antioxidant and antiinflam.atory actions.

关键词： h.ppocam.us neuroprotective Nrf2 gene oxidative stress vanillylacetone

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Is it possible to run after positive affective experiences instead of h.alth.benefits with.ut tackling social inequalities?

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Journal of Sport and h.alth.Science 2024年第6期13卷 756-758页

作者： Raph.el h.O.Araujo Andre O.Werneck Gilm.r m.de Jesus Danilo R.P.Silva Graduation Program.in h.alth.Sciences Londrina State UniversityLondrina 86057-970Brazil Sch.ol of Public h.alth The University of QueenslandBrisbane QLD 4006Australia Center for Epidem.ological Research.in Nutrition and h.alth Department of NutritionSchool of Public HealthUniversidade de S~ao Paulo(USP)Sao Paulo 01246-904Brazil Departm.nt of Psych.logical m.dicine Institute of PsychiatryPsychology and NeuroscienceKing’s College LondonLondon SE58AFUK Public h.alth.Post-Graduate Program State University of Feira de SantanaFeira de Santana 44036-900Brazil Departm.nt of Ph.sical Education Federal University of SergipeSao Cristov~ao 490100-000Brazil

We recently read th. opinion piece titled“Wh. people sh.uld run after positive affective experiences instead of h.alth.benefits”,wh.ch.was carried out by m.ltagliati et al.^(1)and publish.d in th. Journal of Sport and h.alth.*** article started an im.ortant discussion about a ph.sical activity(PA)prom.tion approach.th.t encourages th. use of affective experience to PA *** we agree with.th. auth.rs’point of view and believe th.t its m.ssage can generate an im.ortant insigh. for PA prom.tion,th. auth.rs presented a scenario wh.0}e beh.vior ch.nge seem. to be exclusively dependent on th. *** th. existence of oth.0} barriers to an active lifestyle(e.g.,social inequalities)could enh.nce th. auth.rs’suggested approach.because m.ny people live in conditions th.t reduce th.ir opportunities to engage in affective experiences related to PA;furth.0}m.re,affective experiences can vary depending on individual context.

关键词： prom.tion inequalities furth.0}m.re

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