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MMP-2 Inhibitory Activity of PBS Extract of Guava Leaves

American Journal of Plant Sciences 2021年第12期12卷 1761-1767页

作者： atanu mondal Ramanuj Mukherjee Subhajit mondal Amitava Chatterjee Ramakrishna Mission Vivekananda Educational & Research Institute Kolkata India R G Kar Medical College Kolkata India

PBS (Phosphate Buffed Saline), pH 7.4 extract of Guava leaves (Psidium guajava L.) inhibits the active MMP-2 like molecule both of plant origin as well as active MMP-2 of human (breast cancer patients’ saliva) origin. The zymogram and ELISA were developed of different samples following standard methods. The isolation and identification of the new MMP-2 inhibitor(s) for its molecular characterization is under investigation.

关键词： MMP-2 Inhibitor Guava Leaves Cancer

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Hypobaric-hypoxia Induces Alteration in Microbes and Microbes-associated Enzyme Profile in Rat Colonic Samples

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Biomedical and Environmental Sciences 2013年第10期26卷 869-873页

作者： Chiranjit MAITY atanu ADAK Kuntal GHOSH Bikas R PATI Keshab C mondal Department of Microbiology Vidyasagar University Special Center for Molecular Medicine Jawaharlal Nehru University

Present study deals with the straight impact of hypobaric hypoxia on the quantity and composition of some predominant fecal microflora and its functional aspects. For that, isolated fecal contents of rat were exposed to two different simulated air pressures （70 kPa and 40 kPa） for different time durations （1, 3, and 5 h） and the bacterial community composition was compared with normobaric groups （101.3 kPa）. It was found that the total anaerobes, Escherichio coli, Enterbocters spp., Bi^idobocterium spp., CIostridium spp. were increased whereas total aerobes were decreased at both hypobaric treatments. The increased number of amplicon was detected in the pressure-treated groups than the control that clearly mentioned the disruption of microbiota structure at different simulated hypobaric-hypoxia. The amylase, protease, tannase, 13-glucuronidase, and alkaline phosphatase activities were increased at these atmospheric pressures. Thus, the present investigation demonstrates that the hypobaric hypoxia is an important environmental factor which can strongly modulate the composition of intestinal flora as well as microfiora-derived functional aspects.

关键词： Hypobaric-hypoxia Induces Alteration in Microbes and Microbes-associated Enzyme Profile in Rat Colonic Samples

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Dose specific responses of Anabas testudineus(Bloch)to anthracene(PAH):Haematological and biochemical manifestation

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Emerging Contaminants 2019年第1期5卷 232-239页

作者： Sukhendu Dey Palas Samanta Niladri Sekhar mondal Debraj Kole Arghya Mandal atanu Patra Apurba Ratan Ghosh Ectoxicology Lab Department of Environmental ScienceThe University of BurdwanBurdwan713104West BengalIndia Research Institute of Basic Sciences Incheon National UniversityIncheon22012Republic of Korea Department of Marine Science Incheon National University119 Academy-roYeonsu-guIncheon22012Republic of Korea

Present work compared the toxicological responses of ***(Bloch)to anthracene exposure after treatment with two concentrations i.e.,25%(0.0075 mg/l)and 50%(0.015 mg/l)of LC50 value(0.030 mg/l)for different exposure periods of 1,5,10,15 and 21 day(s).An intensive comparison of the effects was made based on haematological and biochemical parameters of *** responses revealed significant increase in some blood parameters,viz.,WBC,%LYM,MCV,MCH and MCHC but RBC,Hb,PCV and PLT showed decreasing *** responses like CHOLES,PRO,ALB,Trig showed enhanced pattern in the initial exposure of day one then reduced gradually but GLU,GPT,Ca,ALP,HDL,and LDL showed reverse ***,integrated biomarker responses(IBR)indicated that the biochemical parameters are more useful in determining the anthracene stress on fish health than the haematological *** findings of this study suggested that the IBR analysis is a useful tool in monitoring quantitative(dose-specific)as well as qualitative(exposure-specific)responses to toxicological effects of anthracene exposure on fish health.

关键词： Anthracene Haematological and biochemical parameters IBR

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Direct choline administration in semi-intensive pisciculture system: A positive contaminant

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Emerging Contaminants 2021年第1期7卷 22-34页

作者： Subhas Das Sukhendu Dey atanu Patra Arghya Mandal Niladri Sekhar mondal Debnarayan Chowdhury Koushik Ghosh Apurba Ratan Ghosh Ecotoxicology Laboratory Department of Environmental ScienceThe University of BurdwanBurdwanWest BengalIndia Panacea the Aqua Clinic(Aquaculture Laboratory Cum Training Centre)Galsi-II BlockPurba BardhamanDepartment of FisheriesGovt.of West BengalIndia Environmental Science Laboratory Department of Environmental ScienceNetaji Subhas Open UniversitySalt Lake CityWest BengalIndia Aquaculture Laboratory Department of ZoologyThe University of BurdwanBurdwanWest BengalIndia

Present work evaluated the possible impact of choline on metabolic enzymes of cultured fish species into a semi-intensive pond culture system,reared with Catla catla(catla),Labeo rohita(rahu),Clarias batracus(magur)and Anabas testudineus(climbing perch)for 90 days in two *** was added directly into the pond water periodically throughout the experimental *** water quality parameters of each experimental pond were monitored and were analyzed in 15 days interval throughout the experimental tenure in both the *** were compared with treatment as choline-fed and control as non-choline-fed conditions during breeding(June-Aug.)and dry(Nov.-Jan.)*** metabolic enzymes,viz.,LDH(Lactate dehydrogenase),MDH(Malate dehydrogenase),and G6PDH(Glucose-6-phosphate dehydrogenase)activities in the muscle,liver,and AchE(Acetylcholine esterase)activity in the brain and muscle revealed maximum significantly(p<0.01),but ALT(Alanine aminotransferase)and AST(Aspartate aminotransferase)activity showed significant(p<0.01)depletion in the muscle and liver in treatment-breeding(TB)*** fishes under treatment-dry(TD)condition presented significant(p<0.01)higher elevation in HK(Hexokinase)activity in the muscle and ***,the result of principal component analysis(PCA)depicted the positive as well as in some cases a negative correlation among the enzymatic activities both in dry and breeding ***,it can be inferred that choline enrichment in fishes can substantiate the well nutritionally balanced food-flesh for consumption under this farm culture and the choline behaved as a positive contaminant into the pond eco-system under the semi-intensive culture condition.

关键词： Choline Metabolic enzymes Principal component analysis Breeding and dry season

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