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Dose-response effect of pre-exercise carbohydrates under muscle glycogen unavailability:Insights from McArdle disease

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Journal of Sport and Health Science 2024年第3期13卷 398-408页

作者： Pedro L.Valenzuela Alfredo Santalla Lidia B.Alejo Andrea Merlo asuncion bustos Laura Castellote-Belles Roser Ferrer-Costa Nicola A.Maffiuletti David Barranco-Gil Tomas Pinos Alejandro Lucia Physical Activity and Health Research Group(“PaHerg”) Research Institute of Hospital“12 de Octubre”(“imas12”)Madrid 28041Spain Department of Systems Biology University of AlcalaMadrid 28871Spain Department of Sport and Computer Science Section of Physical Education and SportsFaculty of SportUniversidad Pablo de OlavideSevilla 41013Spain EVOPRED Research Group Universidad Europea de CanariasTenerife 38300Spain Faculty of Sport Sciences Universidad Europea de MadridMadrid 28670Spain Gait&Motion Analysis Laboratory Sol et Salus HospitalTorre Pedrera di Rimini(RN)47922Italy Department of Clinical Biochemistry Laboratoris ClınicsHospital Universitari Vall d’HebronBarcelona 08035Spain Human Performance Lab Schulthess ClinicZurich 8008Switzerland Biomedical Research Networking Center on Rare Disorders(CIBERER) Barcelona 08035Spain Mitochondrial and Neuromuscular Disorders Unit Vall d’Hebron Institut de RecercaUniversitat Autonoma de BarcelonaBarcelona 08035Spain

Background:This study aimed to determine the effect of different carbohydrate(CHO)doses on exercise capacity in patients with McArdle disease—the paradigm of“exercise intolerance”,characterized by complete muscle glycogen unavailability—and to determine whether higher exogenous glucose levels affect metabolic responses at the McArdle muscle cell(in vitro)***:Patients with McArdle disease(n=8)and healthy controls(n=9)underwent a 12-min submaximal cycling constant-load bout followed by a maximal ramp test 15 min after ingesting a non-caloric *** a randomized,double-blinded,cross-over design,patients repeated the tests after consuming either 75 g or 150 g of CHO(glucose:fructose=2:1).Cardiorespiratory,biochemical,perceptual,and electromyographic(EMG)variables were ***,glucose uptake and lactate appearance were studied in vitro in wild-type and McArdle mouse myotubes cultured with increasing glucose concentrations(0.35,1.00,4.50,and 10.00 g/L).Results:Compared with controls,patients showed the“classical”second-wind phenomenon(after prior disproportionate tachycardia,myalgia,and excess electromyographic activity during submaximal exercise,all p<0.05)and an impaired endurance exercise capacity(-51%ventilatory threshold and55%peak power output,both p<0.001).Regardless of the CHO dose(p<0.05 for both doses compared with the placebo),CHO intake increased blood glucose and lactate levels,decreased fat oxidation rates,and attenuated the second wind in the ***,only the higher dose increased ventilatory threshold(+27%,p=0.010)and peak power output(+18%,p=0.007).In vitro analyses revealed no differences in lactate levels across glucose concentrations in wild-type myotubes,whereas a doseresponse effect was observed in McArdle ***:CHO intake exerts beneficial effects on exercise capacity in McArdle disease,a condition associated with total muscle glycogen *** of these benefits are dose dependent.

关键词： Endurance Glycogen storage disease Glycogenosis Nutrition Supplement

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Unveiling the brain’s symphony:exploring the necessity and sufficiency of neural networks in behavior control

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Neural Regeneration Research 2025年第1期20卷 186-187页

作者： Fernando Jose bustos Instituto de Ciencias Biomedicas Facultad de Medicina y Facultad de Ciencias de la VidaUniversidad Andres BelloSantiagoChile Millennium Nucleus of Neuroepigenetics and Plasticity(EpiNeuro) SantiagoChile

Since the pioneering work by Broca and Wernicke in the 19th century,who examined individuals with brain lesions to associate them with specific behaviors,it was evident that behaviors are complex and cannot be fully attributable to specific brain areas ***,they involve connectivity among brain areas,whether close or *** that time,this approach was considered the optimal way to dissect brain circuitry and *** pioneering efforts opened the field to explore the necessity or sufficiency of brain areas in controlling behavior and hence dissecting brain ***,the connectivity of the brain and the mechanisms through which various brain regions regulate specific behaviors,either individually or collaboratively,remain largely *** animal models,researchers have endeavored to unravel the necessity or sufficiency of specific brain areas in influencing behavior;however,no clear associations have been firmly established.

关键词： behavior connectivity neural

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Sperm quality in mice acutely treated with parathion

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Asian Journal of Andrology 2000年第2期2卷 147-150页

作者： Cristian Sobarzo Eduardo bustos-Obregon Faculty of Basic Sciences Catholic University of Temuco and Biology of Reproduction Unit Program of Morphology ICBM Faculty of Medicine University of Chile. Santiage 7 Chile.Faculty of Basic Sciences Catholic University of Temuco and Biology of Reproduction Unit Program of Morphology ICBM Faculty of Medicine University of Chile. Santiage 7 Chile.

Aim: To investigate the toxic effect of a single injection of the organophosphorous agropesticide, parathion, on sper-matogenesis in immature male mice. Methods: Seven-day old mice received a single injection of parathion intraperi-toneally at a dose of 1/3 LD_50. The epididymal sperm count, sperm morphology and chromatin thermal stability wereanalyzed 28 and 50 days after injection. Results: Sperm counts were decreased and teratozoospermia and thermal de-naturation of DNA increased after parathion injection. Sperm parameters were changed to a greater extent in younger an-imals, denoting a higher lability of spermatogenic process at its beginning. The damages could recover a long time afterparathion administration. Conclusion: Organophosphorous agropesticides are testicular toxicants, eliciting reversiblecytotoxic and cytogenetic alterations in germ cells.

关键词： epididymis spermatozoa organophosphorous compounds agropesticides spermatogenesis

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Enhanced biological wastewater treatment using sodium alginate-immobilized microorganisms in a fluidized bed reactor

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Water Science and Engineering 2022年第2期15卷 125-133页

作者： Yaneth A.bustos-Terrones Erick R.Bandala Gabriela E.Moeller-Chávez Victoria bustos-Terrones Division of Graduate Studies and Research CONACYT-TecNM-Technological Institute of CuliacánCuliacánSinaloa 80220Mexico Division of Hydrologic Sciences Desert Research InstituteLas VegasNV 89119-7363USA Academic Direction of Environmental Technology Engineering Polytechnic University of the State of MorelosJiutepecMorelos 62550Mexico

In this study,a microbial consortium isolated from an activated sludge tank of a conventional wastewater treatment plant was immobilized using sodium alginate(SA)as a support material for contaminant biodegradation in wastewater.A volume of 500 mL of activated sludge was immobilized in the SA beads(with a mass concentration of 25 g/L).The resulting SA beads were characterized,introduced into a fluidized bed reactor,fed with 1000 mL of the sample,and characterized again after the treatment *** SA-immobilized microorganisms were tested first for degradation of organic matter(expressed as chemical oxygen demand)and total phosphorous in domestic wastewater,achieving removal efficiencies of 71%and 93%,respectively,after 12 ***,the SA-immobilized microorganisms were tested for degradation of a basic blue 9(BB9)textile dye in a condition that simulated textile *** efficiency of the BB9 degradation was found to be as high as 99.5%after 2 *** to these results,SA-immobilized microorganisms were found to be an environmentally friendly and cost-effective alternative for treatment of municipal and industrial wastewater effluents.

关键词： Domestic wastewater Basic blue 9 Immobilized microorganisms Fluidized bed reactor Sodium alginate

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Prevalence of gastroesophageal reflux disease:a population-based cross-sectional study in southern Chile

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Gastroenterology Report 2020年第4期8卷 286-292,I0001,I0002页

作者： Carlos Manterola Luis Grande Luis bustos Tamara Otzen Center of Morphological and Surgical Studies Universidad de La FronteraTemucoChile Department of Surgery Universidad de La FronteraTemucoChile Department of Surgery Hospital del MarUniversitat Auto`noma de BarcelonaBarcelonaSpain Department of Public Health Universidad de La FronteraTemucoChile

Background This study assessed the prevalence of gastroesophageal reflux disease(GERD)in a general adult population from Temuco in southern *** association of GERD with demographic variables was also *** A cross-sectional study among the general population of Temuco in southern Chile was conducted in 2017,using a validated and reliable questionnaire for detecting *** urban area of Temuco,with a population of 245,317 inhabitants(2002 census),was divided into four zones,which were representative of the socioeconomic sectors of the *** sample size was estimated assuming a prevalence of 52.8%,an accuracy of 3.0%,a confidence level of 95.0%,and a design effect of *** sampling was used to build *** prevalence of GERD was determined and associated factors were studied by means of bivariate and multivariate *** A total of 1,069 subjects(47.9%women,median age 40 years)from the selected subareas were *** prevalence of GERD was 44.8%.The most frequently reported symptom was regurgitation(54.8%).One-third of subjects took medication to control symptoms and was considered‘sick’by the instrument,although>68%of them had never sought medical *** was a significant association between GERD and age(P<0.001)and female gender(P=0.001).Conclusions In this population-based study,the prevalence of GERD was high(44.8%).GERD was associated with age and female gender.

关键词： gastroesophageal reflux disease prevalence cross-sectional studies evidence-based medicine clinical epidemiology

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Pesticide soil contamination mainly affects earthworm male reproductive parameters

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Asian Journal of Andrology 2002年第3期4卷 195-199页

作者： Eduardo bustos-Obregón RogerIziga Goicochea Biology of Reproduction Unit. Program of Morphology ICBM Faculty of Medicine University of Chile Saniiago ChileBiology of Reproduction Unit. Program of Morphology ICBM Faculty of Medicine University of Chile Saniiago Chile

Aim: To explore the effect of exposure to commercial Parathion~ (Pc) on the reproductive parameters (sperm and cocoon production and genotoxicity on male germ cells), the survival, the body weight and the gross anatomical changes in Eiseniafoetida. Methods: Three doses of Pc(1478, 739 and 444 mg/kg of soil) and three time intervals of exposure (5, 15 and 30 days) were used. Results: All treated animals were affected. An acute genotoxic effect, revealed by DNA fragmentation (comet assay), was seen by 5 days. Alterations in reproductive parameters were conspicuous in regard to the number of sperm, cocoons and worms born, and the histological observation of the gonads and seminal receptacles. In addition, the body weight and survival rate were decreased. Neuromuscular function was also affected. Conclusion: Earthworms are suitable bioindicators of chemical contamination of the soil, their advantage being their easy and economical handling

关键词： earthworm reproduction soil contamination organophosphoric agropesticide

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Effect of a single dose of malathion on spermatogenesisin mice

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Asian Journal of Andrology 2003年第2期5卷 105-107页

作者： Eduardo bustos-Obregón Patricio González-Hormazabal Biology of Reproduction Unit Program of Morphology ICBM Faculty of Medicine University of Chile Santiago ChileBiology of Reproduction Unit Program of Morphology ICBM Faculty of Medicine University of Chile Santiago Chile

Aim: To observe the acute effect of the organophosphorous insecticide malathion on testicular function in mice. Methods: The effects of a single dose of malathion [240 mg/kg (1/12 LD50)] on plasma acetylcholinesterase (ACE) activity, spermatozoa (epididymal cauda counts and teratozoospermia), testis and plasma testosterone concentration) were evaluated at day 1,8, 16, 35 and 40 after treatment. Results: The sperm count was decreased significantly 24 h after treatment and teratozoospermia was increased at day 35 and 40. The height of the seminiferous epithelium and the diameter of tubular lumen were decreased at day 8. The percentage of tubular blockade was increased between day 8 and 35. A decrease in testosterone plasma level was observed at day 16 after treatment. Conclusion: Malathion damages male reproduction. The depletion of seminiferous tubules and the increase in teratozoospermia may be a genotoxic damage to the renewing spermatogonia, but the possibility of spermatogenic/ spermiogenic disfunction due to a decrease in the plasma testosterone level can not be ruled out.

关键词： testicular toxin malathion seminiferous tubule testosterone

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EpiMap: Fine-tuning integrative epigenomics maps to understand complex human regulatory genomic circuitry

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Signal Transduction and Targeted Therapy 2021年第6期6卷 1627-1629页

作者： Dave S.B.Hoon Negin Rahimzadeh Matias A.bustos Department of Translational Molecular Medicine Division of Molecular OncologySaint John’s Cancer Institute at Providence Saint John’s Health CenterSanta MonicaCAUSA

In a recent paper published in Nature,Boix et al.1 developed an integrated platform on epigenomic maps that represents a significant updated resource in human regulatory circuitry as related to specific organ tissues and cells,as well as specific disease *** EpiMap(for epigenome integration across multiple annotation projects)is a highly valuable compendium comprising 10,000 epigenomic maps across 833 biospecimens and 33 tissue *** distinct biospecimens expanding coverage to life development(embryonic to adult)and different sample types including complex organ tissues as well as primary cells,and established normal and tumor cell *** biospecimen analysis provides a comprehensive view of human genome circuitry making EpiMap a remarkable platform for epigenomic landscapes assessment to the investigator.

关键词： maps tuning Map

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Tuberculous pericarditis-a silent and challenging disease:A case report

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World Journal of Clinical Cases 2022年第6期10卷 1869-1875页

作者： Oscar David Lucero Marlon Mauricio bustos Darwin Jhoan Ariza Rodriguez Juan Camilo Perez Department of Internal Medicine Hospital Universitario San IgnacioBogota 110231Colombia Internal Medicine Resident Pontificia Universidad JaverianaBogota 110231Colombia

BACKGROUND Tuberculous pericarditis(TP)remains a challenge for endemic *** developing countries,one to two percent of patients with pulmonary tuberculosis develops *** SUMMARY A 49-year-old woman presented with dyspnea,chest pain and dry *** physical examination,veiled heart sounds were *** electrocardiogram showed low-voltage complexes and the transthoracic echocardiography revealed a large and free-looking pericardial *** patient was taken for an open *** pericardial fluid revealed high levels of adenosine deaminase and Ziehl-Neelsen stain showed acid-fast *** chain reaction study for Mycobacterium tuberculosis in pericardial fluid was *** patient received tetra conjugate management with adequate clinical response after the first week of treatment and resolution of fever and chest *** In cases of TP,obtaining pericardial fluid and/or pericardial biopsy is the most efficient strategy to confirm the *** diagnosis of this entity will allow physicians to initiate timely treatment,avoid complications and improve the patient's clinical outcome,so we consider the description of this case pertinent and its review in the literature.

关键词： Tuberculosis Pericardial disease Tuberculous pericarditis Pericardial effusion Mycobacterium tuberculosis Case report

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Effect of climate change on drinking water supply in Santiago de Chile

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Research in Cold and Arid Regions 2013年第1期5卷 27-34页

作者： Gerardo Ahumada David bustos María González Department of Civil Engineering Universidad de Chile Santiago Chile

Climate change is likely to increase the occurrence of floods and flashfloods that affect Santiago de Chile＇s drinking water supply system throughout the 21st century. A relationship between flashfloods in the Maipo River--Santiago＇s main raw water source, drainage area in the Maipo Alto Sub Basin and precipitation 48 hours previous to the event was found. Despite having legal guide- lines to guarantee continuity and stability in water supply, Chilean law does not specify the maximum admissible magnitude of an event. A 12% drop of average monthly flow at Maipo en E1 Manzano Station was estimated for the 2035-2065 period due to cli- mate change, meaning water suppliers would not be able to meet 90% monthly water supply security, required by Chilean law. Water suppliers would need to increase their current allotted quota of the Maipo River, from 24.5% to a percentage between 26% and 30% to comply. If the 0 ~C isotherm keeps increasing its elevation through the 21st century, more intense floods could occur because of additional drainage area granted by the elevation of the snow line, even if precipitation does not suffer a significant change. In order to withstand a five day turbidity event, 2 m3/s of groundwater, or any non river source, should be temporarily in- coroorated to the emergency dfinkin~ water production.

关键词： climate change drinking water water availability floods water supply security

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