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Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella

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Journal of Integrative Agriculture 2022年第8期21卷 2372-2383页

作者： WANG Jie ZHANG Qi astrid.lissette barreto sanchez ZHU Bo WANG Qiao ZHENG Mai-qing LI Qing-he CUI Huan-xian WEN Jie ZHAO Gui-ping State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic *** heterophils/lymphocytes(H/L)ratio in the blood reflects the immune system status of *** compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens,after Salmonella infection,aiming to identify the key genes participating in the antibacterial activity in the *** results revealed that,the selected population had stronger(P<0.05)liver resistance to Salmonella typhimurium(ST)than the non-selected *** the selected and non-selected lines,the identified differentiation genes encode proteins involved in biological processes or metabolic pathways that included the TGF-beta signaling pathway,FoxO signaling pathway,and Salmonella infection *** results of the analysis of all identified differentially expressed genes(DEGs)of spleen revealed that the G protein-coupled receptor(GPCR)and insulin-like growth factor(IGF-I)signaling pathways were involved in the Salmonella infection *** analysis of DEGs and FST(fixation index),identified candidate genes involved in Salmonella infection pathway,such as GPR39,NTRK2,and *** extensive genomic changes highlight the polygenic genetic of the immune response in these chicken *** genes related to the immune performance are differentially expressed in the selected and non-selected lines and the selected lines has a higher resistance to Salmonella.

关键词： heterophils/lymphocytes selective sweep RNA-seq Salmonella typhimurium disease-resistance

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基于生理和转录组学分析的北京油鸡和广明2号白羽肉鸡抗热应激机制对比研究

基于生理和转录组学分析的北京油鸡和广明2号白羽肉鸡抗热应激机...

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作者： astrid.lissette barreto S(?)NCHEZ 中国农业科学院

学位级别：硕士

热应激给肉鸡产业带来严重经济损失,耐热基因的挖掘和新品种的培育成为产业亟待解决的难题。热应激会引起机体产生各种生理变化,例如氧化应激、酸碱失衡和抑制免疫能力,从而导致死亡率增加和饲料效率、采食量、产蛋量降低,同时也会影响... 详细信息

热应激给肉鸡产业带来严重经济损失,耐热基因的挖掘和新品种的培育成为产业亟待解决的难题。热应激会引起机体产生各种生理变化,例如氧化应激、酸碱失衡和抑制免疫能力,从而导致死亡率增加和饲料效率、采食量、产蛋量降低,同时也会影响肉和蛋的质量,导致动物生产性能低下,进而造成经济损失。已有研究表明,地方鸡种比商业品种具有更强的抗热应激能力。然而,在地方鸡种中表现出来的耐热性的潜在机制仍未阐明。因此,研究中国地方鸡种和快速生长型商业品种的抗热应激分子机制,以促进具有抗热应激能力的肉鸡新品种培育,就显得尤为重要。本研究对北京油鸡(BY)和广明2号白羽肉鸡(GM)的热应激反应进行了表型、生理、肝脏转录组比较分析和加权基因共表达网络分析(WGCNA)。本研究的目的是评估热应激对两个品种的表型和生理参数的影响,评估其耐热性并确定这两个品种与热应激相关的潜在驱动基因和枢纽基因。我们选取80只BY和60只GM在25日龄进行为期3天(33℃,8小时/天)的热应激试验。每个品种分为对照组(Ctl;24°C,60%相对湿度)和热应激组(HS;33°C,60%相对湿度)。即,50只BY和30只GM被随机分配到同一环境控制室(ECCR)中饲养,其余30只BY和30只GM被分配到Ctl组在另一个ECCR内单独饲养。在28日龄时称重,采血用于血清学指标检测,并收集肝脏组织用于转录组学分析。研究结果表明,GM比BY更容易受热应激环境影响。热应激对BY的体重、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)和异嗜细胞/淋巴细胞比率(H/L)未造成显著影响,但与Ctl组相比,GM的HS组的体重显著下降,H/L和T-AOC显著升高。品种之间的比较分析发现,在热应激下,GM的H/L和T-AOC显著高于BY,表明GM更容易受热应激影响。肝脏转录组中鉴定到BY和GM的热应激组与对照组之间的差异表达基因(DEGs)分别有365和382个。同时,通路富集分析发现,BY中的DEGs主要富集在代谢活性相关的信号通路上,而GM的DEGs富集的信号通路主要与炎症反应相关。BY中鉴定到的CPT1A、ANGPTL4和G6PC以及GM中鉴定到的HSP90B1、HSPA5被确定为与HS有关的候选基因。WGCNA揭示TLR7、AR、BAG3为枢纽基因,可能在HS中发挥重要作用。该研究从转录组层面系统解析了地方鸡种BY和商业品种GM在抗热应激方面的机制差异。此外,本研究鉴定到的与热应激相关的候选基因,也为培育抗热应激商业肉鸡新品种奠定了理论基础。

关键词：热应激北京油鸡广明2号转录组 WGCNA

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基于脾脏转录组筛选北京油鸡和广明白鸡抗热应激相关功能基因

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畜牧兽医学报 2023年第5期54卷 1905-1914页

作者：王子渲王巧张锦 astrid.lissette barreto sanchez 郑麦青李庆贺崔焕先安炳星赵桂苹文杰李和刚青岛农业大学动物科技学院青岛266109 中国农业科学院北京畜牧兽医研究所北京100193

旨在通过对北京油鸡(BY)和“广明2号”白羽肉鸡B系(广明白鸡,GM)在热应激和正常饲养条件下的脾脏转录组进行分析,鉴定两鸡种在应对热应激时的差异表达基因和信号通路,解析不同鸡种耐热性能差异的分子调控机制。试验动物采用相同饲养条... 详细信息

旨在通过对北京油鸡(BY)和“广明2号”白羽肉鸡B系(广明白鸡,GM)在热应激和正常饲养条件下的脾脏转录组进行分析,鉴定两鸡种在应对热应激时的差异表达基因和信号通路,解析不同鸡种耐热性能差异的分子调控机制。试验动物采用相同饲养条件下的25日龄北京油鸡和广明白鸡,测定异嗜性粒细胞与淋巴细胞的比值(H/L)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)等表型数据,采集脾脏组织进行转录组测序(RNA-Seq)。基于RNA-Seq数据,结合表型进行加权基因共表达网络分析(WGCNA),筛选出与性状相关性较高的模块与基因。脾脏转录组测序结果表明,热应激组相比于对照组,北京油鸡中共鉴定到313个差异表达基因,其中169个上调表达,144个下调表达;广明白鸡中鉴定到235个差异表达基因,其中152个上调表达,83个下调表达。利用WGCNA分析,在北京油鸡中筛选出2个与H/L相关性较高的模块,在广明白鸡中筛选出4个与H/L和T-AOC强相关的模块。通过筛选模块中的Hub基因,发现两品种中均存在TRIM 29基因,说明该基因可能在抗热应激方面发挥重要作用。本研究通过转录组分析,揭示了不同鸡种在热应激下基因的表达差异规律,并鉴定到与耐热相关的候选基因,为进一步研究鸡的耐热机制提供了新的思路和线索。

关键词：北京油鸡广明白鸡热应激差异表达基因加权基因共表达网络分析

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Astrocytes and retrograde degeneration of nigrostriatal dopaminergic neurons in Parkinson’s disease:removing axonal debris

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Translational Neurodegeneration 2021年第4期10卷 554-572页

作者： Ingrid Morales Ricardo Puertas-Avendaño Alberto sanchez Adrian Perez-barreto Clara Rodriguez-Sabate Manuel Rodriguez Laboratory of Neurobiology and Experimental Neurology Department of Basic Medical SciencesFaculty of MedicineLa Laguna UniversityLa LagunaTenerifeCanary IslandsSpain Center for Networked Biomedical Research on Neurodegenerative Diseases(CIBERNED) MadridSpain

Objective:The dopaminergic nigrostriatal neurons(DA cells)in healthy people present a slow degeneration with aging,which produces cellular debris throughout *** 2%-5%of people present rapid cell degeneration of more than 50%of DA cells,which produces Parkinson's disease(PD).Neuroinflammation accelerates the cell degeneration and may be critical for the transition between the slow physiological and the rapid pathological degeneration of DA cells,particularly when it activates microglial cells of the medial forebrain bundle near dopaminergic *** synaptic debris produced by DA cell degeneration may trigger the parkinsonian neuroinflammation,this study investigated the removal of axonal debris produced by retrograde degeneration of DA cells,paying particular attention to the relative roles of astrocytes and ***:Rats and mice were injected in the lateral ventricles with 6-hydroxydopamine,inducing a degeneration of dopaminergic synapses in the striatum which was not accompanied by non-selective tissue damage,microgliosis or *** possible retrograde degeneration of dopaminergic axons,and the production and metabolization of DA-cell debris were studied with immunohistochemical methods and analyzed in confocal and electron microscopy ***:The selective degeneration of dopaminergic synapses in the striatum was followed by a retrograde degeneration of dopaminergic axons whose debris was found within spheroids of the medial forebrain *** spheroids retained mitochondria and most(e.g.,tyrosine hydroxylase,the dopamine transporter protein,and amyloid precursor protein)but not all(e.g.,α-synuclein)proteins of the degenerating dopaminergic *** showed initial(autophagosomes)but not late(lysosomes)components of autophagy(incomplete autophagy).These spheroids were penetrated by astrocytic processes of the medial forebrain bundle,which provided the lysosomes needed to continue the degradation of dopaminergic debri

关键词： Astrocyte Phagocytosis Parkinson's disease Dying-back degeneration Medial forebrain bundle Spheroid

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Dengue Climate Variability in Rio de Janeiro City with Cross-Wavelet Transform

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Journal of Environmental Protection 2022年第3期13卷 261-276页

作者： Suellen Araujo Franco dos Santos Hugo Abi Karam Augusto José Pereira Filho Jú lio Cesar barreto da Silva Jose Luis Flores Rojas Julio Migue Angeles Suazo Isela Leonor Vá squez Panduro Cesar Arturo sanchez Peñ a Departamento de Meteorologia Instituto de Geoci&ecirc ncias Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brasil Departamento de Ciê ncias Atmosfé ricas Instituto de Astronomia Geofísica e Ci&ecirc ncias Atmosfé ricas Universidade de S&atilde o Paulo (IAG-USP) S&atilde o Paulo Brasil Programa de Pós-Graduaç ã o em Ciê ncias Ambientais Universidade Estadual do Rio de Janeiro (UERJ) Rio de Janeiro Brasil Instituto Geofísico do Peru (IGP) Lima Per&uacute Facultad de Ingeniería Forestal y Ambiental. Universidad Nacional Autó noma de Tayacaja Daniel Herná ndez Morillo (UNAT) Pampas Per&uacute Universidad Tecnoló gicadel Perú Faculdad de Engenier&iacute a Industrial Lima Per&uacute Centro de Previsã o do Tempo e Estudos Climá ticos Cachoeira Paulista Brasil Programa de Pó s-Graduaç ã o em Computaç ã o Aplicada Instituto Nacional de Pesquisas Espaciais (INPE) S&atilde o José dos Campos S&atilde o Paulo SP Brasil

Dengue is one of the most prominent tropical epidemic diseases present in the Rio de Janeiro city and Southeast part of Brazil, due to the widespread conditions of occurrence of the dengue vector, the mosquito Aedesaegypti, such as high-temperature days interlaced with afternoon or nocturnal rainstorms in summer. This work has the objective of investigating the relationships between variabilities of the El Ni?o-South Oscillation (ENSO) and greater epidemics of dengue in Rio de Janeiro city. To accomplish this goal, the analysis and signal decomposition by cross-wavelet transform (WT) was applied to obtain the cross variability associated with variations of power and phase of both signals by characteristic periods and along with the time series. Data considered in the analysis are (the decimal logarithm of normalized value) of the monthly available notifications of dengue worsening, provided by the public health system of Brazil, and the Southern Oscillation Index (SOI) Ni?o 3.4 data, provided by the National Oceanic and Atmospheric Administration (NOAA), in the period 2000-2017. A maximum cross-wavelet power close to 0.45 was obtained for the representative period of 1 year and also to periods between 3 and 4 years, associated with the positive phase of the SOI index (i.e. , La Ni?a) or with a transition to the positive phase. The evolution of the combined variability of SOI and dengue can be expressed by progressive differences in phase along the time, eventually resulting in yielding phases (i.e., La Niña-Dengue epidemic).

关键词： Dengue Fever Cross-Wavelet Transform Analysis El Niño-Southern Oscillation Index

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