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Reactions of Maruca Resistant Transgenic Cowpea to Cowpea Aphid-Borne Mosaic Virus

Agricultural Sciences 2024年第7期15卷 742-753页

作者： Mounyratou Rabo Moustapha Ouédraogo Orokia Coulibaly assita traoré-barro Salimata traoré Teyioue Benoit Joseph Batieno Chantal Kaboré-Zoungrana Aboubacar Toguyeni Oumar traoré Laboratoire National de Bioscurit Agence Nationale de Bioscurit (ANB) Ouagadougou Burkina Faso Institut de lEnvironnement et Recherches Agricoles Dpartement Productions Vgtales Ouagadougou Burkina Faso Laboratoire dEtudes et de Recherches sur les Ressources Naturelles et les Sciences de lEnvironnement (LERNSE) Universit Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire De Gntique et Amlioration Des Plantes (LAGAMEP) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire De Protection et Dfense Des Cultures (LAPRODEC) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso

Cowpea (Vigna unguiculata L. [Walp.]) in one of the main grain legumes contributing to food security and poverty alleviation in Sub-Saharan Africa. To control the highly damaging legume pod borer Maruca vitrata F., transgenic cowpea lines expressing the insecticidal Cry1Ab Bt protein were developed. In this study, we evaluated the impact of Cry1Ab transgene expression on the susceptibility of four cowpea lines (named IT97K-T, IT98K-T, Gourgou-T and Nafi-T) and their respective non-transgenic near isogenic lines (IT97K, IT98K, Gourgou and Nafi) to Cowpea aphid-borne mosaic virus (CABMV) in greenhouse conditions. In a preliminary quality control test by enzyme-linked immunosorbent assay, the presence of Cry1Ab protein in transgenic seed lots ranged from 59% to 72%, with no significant differences among the lines (χ2 = 3.26;p = 0.35). Upon virus inoculation, all cowpea lines exhibited mosaic symptoms with similar severity between 7- and 11-day post-inoculation. No significant differences were observed in symptom severity. Significant differences were found between cowpea lines for time of symptom onset, virus accumulation in plants and days to 50% flowering. However, while comparing pairs of transgenic lines and corresponding non-transgenic lines, virus accumulation showed not significant differences whatever the pair. Time of symptom onset and days to 50% flowering did not also differ significantly between pairs of cowpea lines except Nafi/Nafi-T in which transgenic Nafi-T showed earlier symptoms (7.4 ± 0.7 vs. 8.9 ± 0.8 days post-inoculation) and shorter flowering time (37.3 ± 0.6 vs. 42 ± 1.7 days after sowing). Overall, these findings improve our understanding of the effects of Cry1Ab gene mediated genetic modification on cowpea infection by Cowpea aphid-borne mosaic virus, with potential implications for environmental safety assessment.

关键词： Cowpea Cry1Ab Cowpea Aphid-Borne Mosaic Virus Environmental Safety

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Reactions of Maruca Resistant Transgenic Cowpea to Cowpea Aphid -Borne Mosaic Virus

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Agricultural Sciences（农业科学） 2024年第7期 742页

作者： Mounyratou Rabo Moustapha Ouédraogo Orokia Coulibaly assita traoré-barro Salimata traoré Teyioue Benoit Joseph Batieno Chantal Kaboré-Zoungrana Aboubacar Toguyeni Oumar traoré

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Characterization of Seed Storage Proteins in Eight Bambara Groundnut Landraces in Burkina Faso

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Agricultural Sciences 2023年第9期14卷 1268-1276页

作者： assita traoré-barro Emmanuel A. M. Thiombiano Karidiatou Gnankambary Mounyratou Rabo Orokia Coulibaly Salimata traoré Oumar traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité Ouagadougou Burkina Faso Laboratoire de Biochimie et de Chimie Appliquée Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The Bambara groundnut Vigna subterranea (L.) Verdc. is a drought-resistant indigenous African grain legume with significant nutritional and agronomic potential. This study aimed to characterize the seed storage proteins of eight Bambara groundnut landraces. Seeds of Bambara groundnut landraces were collected from local markets in Burkina Faso, and total soluble protein as well as protein fractions were extracted. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content of the seeds was found to be 18.46%, with variations ranging from 17.69% to 19.17% among the different landraces. Most of the protein content was soluble, constituting approximately 87.04% of the total crude protein. Albumin fraction was the most dominant, representing about 95.42% of the total soluble proteins. The globulin, prolamin and glutelin fractions accounted for 1.82%, 0.13% and 1.17% of the soluble proteins, respectively. The findings provide valuable insights into the protein composition of Bambara groundnut landraces and contribute to our understanding of its nutritional potential, laying the groundwork for further research on crop improvement and sustainable agriculture practices.

关键词： Bambara Groundnut Landraces Protein Protein Fractions

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Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences 2023年第12期14卷 1453-1463页

作者： Mounyratou Rabo Teyioue Benoit Joseph Batieno assita traoré-barro Salimata traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité (ANB) Ouagadougou Burkina Faso Institut de l’Environnement et Recherches Agricoles Département Productions Végétales Ouagadougou Burkina Faso Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE) Université Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关键词： Cowpea Protein Cry1Ab Protein Fractions

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Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences（美国植物学期刊） 2023年第12期

作者： Mounyratou Rabo Teyioue Benoit Joseph Batieno assita traoré-barro Salimata traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar traoré

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Characterization of Seed Storage Proteins in Eight Bambara Groundnut Landraces in Burkina Faso

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Agricultural Sciences（农业科学） 2023年第9期

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Determination of the Electrical Parameters of a Solar Cell in Steady State

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Open Journal of Applied Sciences 2023年第10期13卷 1834-1843页

作者： Omar Diallo Sadio Mamadou Kouyaté Papa Touty traoré Fabé Idrissa barro Department of Physics Semiconductors and Solar Energy Laboratory Faculty of Science and Technique Cheikh Anta Diop University Dakar Senegal

Photovoltaic solar energy can be obtained by using several types of technologies, including silicon solar cells. The characterization of its solar cells makes it possible to know them better. This article presents, on the one hand, the work that has been carried out on these cells. On the other hand, a theoretical study of the cell under illumination using Lambert’s W function. On the basis of the electrical parameters provided by the manufacturer, the parameters such as the series and shunt resistances and the electrical quantities such as the photocurrent and the photovoltage, are determined and studied according to the ideality factor of the diode. From the results obtained the shunt resistance increases when the ideality factor increases, the series resistance decreases very weakly.

关键词： Solar Cell Series Resistance Shunt Resistance Ideality Factor Lambert Function

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Detection and Serological Characterization of Rice Yellow Mottle Virus in Central African Republic

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Agricultural Sciences 2016年第12期7卷 911-919页

作者： Régis Dimitri Sokpé Longué Innocent Zinga Silla Semballa Nicolas barro Oumar traoré Crop Production Department Institute of Environment and Agricultural Research Ouagadougou Burkina Faso Life Science Department University of Bangui Bangui Central African Republic Department of Biochemistry and Microbiology University of Ouagadougou I-Professor Joseph Ki-Zerbo Ouagadougou Burkina Faso

Serological and biological detection of Rice Yellow Mottle Virus (RYMV) in leaf samples belonging from cultivated rice species and wild grasses revealed 201 positive detections. All these isolates occurred exclusively on cultivated rice species (Oryza sativa L. and O. glaberrima Steud). A relationship was found between cultural practices and presence of RYMV in fields (X2 = 108.83, df = 1, P

关键词： RYMV Detection Serological Characterization Central African Republic

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Imperata yellow mottle virus: An Emerging Threat to Maize, Sorghum and Pearl Millet in Burkina Faso

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Agricultural Sciences 2017年第5期8卷 397-408页

作者： Moustapha Koala Valentin Stanislas Edgar traoré Drissa Sérémé Bouma James Neya Christophe Brugidou Nicolas barro Oumar traoré Laboratoire Mixte Internationale (LMI) Patho-Bios Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Universite Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso UMR IPME (UM IRD CIRAD) Institut de Recherche pour le Développement IRD Montpellier France

Imperata yellow mottle caused by Imperata yellow mottle virus (IYMV) of the genus Sobemovirus was first characterized on Imperata cylindrical and Zea mays in Burkina Faso. The disease has been reported in several localities of the country but its ecology and epidemiology are poorly known. In particular, only I. cylindrical and maize have been reported within IYMV host range. The aim of this study was to investigate the experimental host range of the virus. Mechanical inoculation of a mixture of four IYMV isolates to 18 plant species, including four crops (maize, rice, sorghum and pearl millet) and 14 wild grasses showed clear mottle symptoms in maize, sorghum and pearl millet and two wild grass species (Setaria verticillata and Rottboellia exaltata). Symptom development was confirmed by Enzyme-linked immunosorbent assay and reverse transcription–polymerase chain reaction (RT-PCR). Infection of crop species by IYMV depended on cultivars tested. Therefore, proportions of positive cultivars were 16/36 in maize, 4/10 in sorghum and 4/9 in pearl millet, respectively. Studies on virus-host interactions using individual virus isolates showed two pathogenic patterns. Three out of the four isolates tested infected all plant species and cultivars. In contrast, the fourth IYMV isolate could infect only one maize cultivar. These results expand the previously known host range of IYMV from two to five species, indicating a narrow host range. Among the new characterized host species, sorghum and pearl millet are important cereal crops. Therefore, Imperata yellow motte disease is a potential threat for the cereal crop production and its ecology and epidemiology should be thoroughly investigated.

关键词： IYMV Sobemovirus Host Range Virus-Host Interactions

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Impact of Cowpea mottle viruson the Growth and Yield of Bambara Groundnut (Vigna subterranean(L.) Verdc.)

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American Journal of Plant Sciences 2018年第10期9卷 2053-2062页

作者： Elisabeth Zongo Bouma James Néya Valentin Stanislas Edgar traoré Essowé Palanga Jean Zabré Nicolas barro Oumar traoré Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA Ouagadougou Burkina Faso Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire National de Biosécurité Ouagadougou Burkina Faso

Bambara groundnut [Vigna subterranean (L.) Verdc] is a food legume with high potential for food security in Sub-Saharan Africa. However, in addition to being a neglected crop, its production is limited by several constraints among which viral diseases are most cited. In order to contribute to the improvement of Bambara groundnut in Burkina Faso, local accessions of the crop were screened for resistance to Cowpea mottle virus (CPMoV), one of the most damaging viruses in grain legumes. Seven local accessions (C1 to C7) from two agro-ecological zones were evaluated by mechanical inoculation in field conditions in 2016 and 2017. The infected plants exhibited various symptoms of chlorosis, leaf deformation, growth retardation and plant stunting. CPMoV caused a significant reduction in the number of flowers and pods. As a result, grain yield was reduced by 49.5% to 83.9% depending on the accessions. The impact of the virus in yield loss was lowest in accessions C6 and C7 which indicated their possible used in the management of Cowpea mottle virus disease in bambara groundnut.

关键词： Bambara Groundnut Cowpea mottle virus Impact Yield Loss

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