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Allogenic Stem Cell Transplantation and COVID-19 Antibodies: Mechanistic Insights and Recipient Concerns

Open Journal of Immunology 2024年第2期14卷 16-32页

作者： Amna ume. asma ume. khayam Nasar Khan David Lawrence Greene Umm E. Habiba Sabiha Shamim Pak-American Hospital Pvt. Ltd. Islamabad Pakistan R3 Medical Research LLC Scottsdale USA Bello Bio Labs and Therapeutics (SMC) Pvt. Ltd. Islamabad Pakistan Department of Biochemistry Rawalpindi Medical University Rawalpindi Pakistan Bello Bio LLC Scottsdale USA

Collecting umbilical cord stem cells is widely practiced due to its nume.ous benefits. Over the past decade, umbilical cord stem cells (UCSCs) have shown effectiveness in treating various conditions, such as bone pathologies, neuropsychiatry disorders, hereditary diseases, and metabolic disorders. However, factors like immunization affect the quantity and quality of cord harvesting. Studies suggest that antibodies from the mother pass through the umbilical cord to protect the infant against infections. Cleaning the umbilical cord before stem cell extraction is crucial to maintain sterility and cell integrity. Vaccinating a female donor, including for COVID-19, typically does not directly affect the stem cells. Although vaccines aim to trigger an immunological response, they generally do not affect the donor’s stem cells.

关键词： Vaccination Stem Cell Allogeneic COVID Antibodies

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Detection of Pathogenic Bacteria <i>Staphylococcus aureus</i>and <i>Salmonella</i>sp. from Raw Milk Samples of Different Cities of Pakistan

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Natural Science 2020年第5期12卷 295-306页

作者： Syeda asma Bano Munazza Hayat Tayyaba Samreen Mohammad Asif ume.Habiba Bushra Uzair Department of Microbiology University of Haripur Haripur Pakistan Pakistan Agricultural Research Council P. & D. Islamabad Pakistan Department of Wild Life and Management University of Haripur Haripur Pakistan Department of Biosciences International Islamic University Islamabad Pakistan

Food-borne diseases are the main public health problem throughout the world. Milk is important component of human diet including fats, proteins, vitamins and minerals. It is a best source of calcium and phosphorus. Different types of pathogenic bacteria like S. aureus and Salmonella enter in milk and then multiply, after multiplication they become active in causing diseases. These bacteria create serious problems for human health. This study aimed to isolate and identify pathogenic bacteria Staphylococcus aureus and Salmonella from raw milk samples of different cities of Pakistan. Primary screening of raw milk samples was done on the basis of morphological, cultural and biochemical techniques. The final identification was made using 16SrRNA sequence analysis. A total of 200 raw milk samples were collected from different cities of Pakistan. Selective medium xylose lysine deoxycholate agar (XLD) and Mannitol salt agar were used for the identification of Salmonella sp. and S. aureus. Staphylococcus aureus produced yellow colonies with yellow zones on Mannitol salt agar. Staphylococcus aureus exhibited gram-positive character with purple coloration and it was detected as cocci-shaped. Biochemically 91 (45%) samples enhibited Catalase, Coagulase, DNase, Urease, Citrate, fermentation tests positive and indole, oxidase and H2S tests negative with nonmotile character, indicating the presence of Staphylococcus aureus. Salmonella sp. was detected as gram negative rods with pink coloration on gram staining. Biochemically 87 (43%) samples revealed catalase, citrate, H2S and fermentation tests positive while oxidase, DNase, Indole and urease tests negative, indicating the presence of Salmonella sp. in these samples. Of the 200 samples tested, 43% were positive for Salmonella, while 45% samples were contaminated with S. aureus. The 16SrRNA sequence analysis confirmed the results of biochemical and cultural characterization by depicting 99% identity of samples with S. aureus and 9

关键词： Raw Milk Staphylococcus aureus Salmonella sp. Mannitol Salt Agar Xylose Lysine Deoxycholate

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