AIM: To evaluate GenoT ype methicillin-resistant Staphylococcus aureus(MRSA) Direct assay and cultivation for the identification of MRSA by using mec A polymerase chain reaction(PCR) as the "gold standard" ***: In tot...
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AIM: To evaluate GenoT ype methicillin-resistant Staphylococcus aureus(MRSA) Direct assay and cultivation for the identification of MRSA by using mec A polymerase chain reaction(PCR) as the "gold standard" ***: In total of 61 nasal specimens from patients at the intensive care unit were studied by GenoType MRSA Direct test, conventional culture method and automated bacterial identification system. The results of Geno Type MRSA Direct assay were compared to conventional culture method the identification of MRSA and mec A gene PCR as the "gold standard" method. The sensitivity, specificity, positive predictive value and negative predictive value were ***: In total, 61 specimens were studied. Fiftyfour specimens(88.5%) were negative by all three methods. Six swabs(9.8%) were found positive by GenoT ype MRSA Direct test, conventional culture method and automated bacterial identification system. The presence of mec A in these strains was confirmed by PCR. One swab sample was negative for culture meth-ods but MRSA and mecA gene were detected by GenoType MRSA Direct test and mec A PCR respectively. GenoT ype MRSA Direct test had a sensitivity of 100%(6/6) and a specificity of 100%(55/55), with a positive predictive value of 100% and a negative predictive value of 98%. Culture method of MRSA had a sensitivity of 83.3%(5/6) and a specificity of 98.2%(55/56). CONCLUSION: It was found that the GenoT ype MRSA Direct assay, which is a rapid and accurate test, is of the same sensitivity and specificity with mec A PCR. The GenoT ype MRSA Direct assay can be a better tool for rapid and accurate detection of MRSA in diagnostic laboratories.
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