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Conversion of Waste Parasitic Insect(Hylobius abietis L.)into Antioxidative,Antimicrobial and Biodegradable Films

Journal of Renewable materials 2019年第3期7卷 215-226页

作者： murat Kaya Idris Sargin Povilas mulerčikas Jalel Labidi asier m.salaberria Yavuz S.Cakmak Sonata Kazlauskaitė Demet Erdonmez Vykintas Baublys Department of Biotechnology and molecular Biology Aksaray University68100AksarayTurkey Instituteof Biology and Plant Biotechnology Aleksandras Stulginskis UniversityLT-53361Lithuania Biorefinery Processes Research Group Department of Chemical and Environmental EngineeringUniversity of the Basque Country(UPV/EHU)Plaza Europa 120018Donostia-San SebastianSpain Department of Biology Faculty of Science and LettersAksaray University68100AksarayTurkey Department of Biology Vytautas Magnus UniversityLT-44404 KaunasLithuania

Hylobius abietis is a plant parasitic insect belonging to the order Coleoptera and which causes severe damages to coniferous forests in Northern and Eastern *** current study is aimed to provide a new viewpoint into the waste of this insect by producing *** insect corpses consisted of 27.9%chitin and 86.2%of the chitin was converted into the ***-IR spectra analyses confirmed the purity and the deacetylation degree of the produced chitosan(molecular weight of chitosan;7.3 kDa).This chitosan exhibited antimicrobial activity against 18 bacterial ***,biodegradable chitosan composite films with β-carotene were *** activity of chitosan films were found to be higher than chitosan gels;andβ-carotene incorporation further increased the antioxidative properties of the chitosan *** study demonstrated that the waste of parasitic insect like *** can be evaluated as a source for production of biodegradable and edible chitosan-based films for applications in food coating.

关键词： Hylobius abietis biowaste chitin chitosan antioxidant

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Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures

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Journal of Integrative Plant Biology 2015年第4期57卷 357-372页

作者： Hugo mélida asier Largo-Gosens Esther Novo-Uzal Rogelio Santiago Federico Pomar Pedro García Penélope García-Angulo José Luis Acebes Jesús álvarez Antonio Encina Plant Physiology Laboratory Faculty of Biological and Environmental SciencesUniversity of León Centre for Plant Biotechnology and Genomics (CBGP) Politechnical University of Madrid Department of Plant Biology University of Murcia Plant Biology and Soil Sciences Department Faculty of BiologyUniversity of Vigo Environmental Agrobiology Soil and Plant Quality (University of Vigo)Associated Unit to Biological Mission of Galicia (CSIC) Department of Animal Biology Plant Biology and EcologyUniversity of A Coru?a Department of molecular Biology(área de Genética) Faculty of Biological and Environmental SciencesUniversity of León

maize （Zea mays L.） suspension-cultured cells with up to 7o% less cellulose were obtained by stepwise habituation to dichlobenil （DCB）, a cellulose biosynthesis inhibitor. Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared （FTIR） spectroscopy. Cell wall compositional analysis indicated that the cellulosedeficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin. Thioacidolysis/GC-mS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to shortterm DCB-treatments. Gene expression analysis of these ceils indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the iasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.

关键词： Cellulose DCB dichlobenil ectopic lignin maize

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