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Next-generation Sequencing of the Arabidopsis Meiotic Transcriptome and Genomic Variations before and after Meiotic Recombination

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Next-generation Sequencing of the Arabidopsis Meiotic Transc...

Arabidopsis PTD Is Required for Type Ⅰ Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

第十二届全国植物基因组学大会

作者： asela j.wijeratne Department of Biology and the Huck Institutes of the Life Sciences the Pennsylvania State UniversityUniversity ParkPA 16802USA Intercollege Graduate Program in Plant Biology the Pennsylvania State UniversityUniversity ParkPA 16802USA Current Address:Molecular and Cellular Imaging Center(MCIC) Ohio State University/OARDCWoosterOH 44691USA

Meiosis is essential for eukaryotic sexual reproduction and requires both specific and non-specific gene *** addition,meiotic recombination redistributes genome variations and affects population allelic frequencies every *** obtain clues about global meiotic expression patterns and gene activities,we performed RNA sequencing of the Arabidopsis male meiocyte,detecting the expression of～20,000 genes, with>800 genes that are meiocyte-preferentially expressed,including known meiotic *** 3,378

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journal of Genetics and Genomics 2014年第3期41卷 165-175页

作者： Pingli Lu asela j.wijeratne Zhengjia Wang Gregory P.Copenhaver Hong Ma Institute of Plant Biology Institute of GeneticsSchool of Life SciencesFudan University Department of Biology and the Huck Institutes of the Life Sciences Pennsylvania State UniversityUniversity Park Molecular and Cellular Imaging Center(MCIC) Ohio State University/OARDCWooster School of Forestry and Biotechnology Zhejiang A&F University Department of Biology and the Carolina Center for Genome Sciences University of North Carolina at Chapel Hill Lineberger Comprehensive Cancer Center University of North Carolina School of Medicine

In eukaryotes, crossovers together with sister chromatid cohesion maintain physical association between homologous chromosomes, ensuring accurate chromosome segregation during meiosis I and resulting in exchange of genetic information between homologues. The Arabidopsis PTD （Parting Dancers） gene affects the level of meiotic crossover formation, but its functional relationships with other core meiotic genes, such as AtSP011-1, AtRAD51, and AtMSH4, are unclear; whether PTD has other functions in meiosis is also unknown. To further analyze PTD function and to test for epistatic relationships, we compared the meiotic chromosome behaviors ofAtspoll-1 ptd and Atrad51 ptd double mutants with the relevant single mutants. The results suggest that PTD functions downstream of AtSP011-1 and AtRAD51 in the meiotic recombination pathway. Furthermore, we found that meiotic defects in rck pM and Atmsh4 ptd double mutants showed similar meiotic phenotypes to those of the relevant single mutants, providing genetic evidences for roles of PTD and RCK in the type I crossovers pathway. Moreover, we employed a pollen tetrad-based fluorescence method and found that the meiotic crossover frequencies in two genetic intervals were significantly reduced from 6.63% and 22.26% in wild-type to 1.14% and 6.36%, respectively, in the ptd^2 mutant. These results revealed new aspects of PTD function in meiotic crossover formation.

关键词： PTD Meiosis MSH4 Crossover Recombination frequency

Establishing a regulatory gene network for Arabidopsis anther cell differentiation

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Establishing a regulatory gene network for Arabidopsis anthe...

Spatial and temporal distribution of cyanobacteria in Batticaloa Lagoon

中国遗传学会植物遗传和基因组学专业委员会2007年学术研讨会

作者： asela j．wijeratne Reka Albert Intercollege Graduate Program in Plant Biology the Pennsylvania State UniversityUniversity ParkPA 16802 Department of Biology and the Huck Institutes of the Life Sciencesthe Pennsylvania State UniversityUniversity ParkPA 16802 Department of Physics the Pennsylvania State UniversityUniversity ParkPA 16802

The flowering plant anther contains highly specialized reproductive and somatic ceils required for male *** studies have uncovered several mutants that are defective for anther development,including sporocyteless (spl...

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journal of Environmental Sciences 2016年第9期28卷 211-218页

作者： jalaldeen Mohamed Harris Periyathamby Vinobaba Ranil Kavindra asela Kularatne Champika Ellawala Kankanamge Department of Zoology Eastern UniversityVantharumoolaiChenkaladiSri Lanka MAS Capital(Private)Limited 10th FloorAitken Spence Tower If315Vauxhall StreetColombo 2Sri Lanka MAS Active Trading(Put)Limited No.231D.M.Colombage MawathaNatualaSri Lanka Department of Civil and Environmental Engineering Faculty of EngineeringUniversity of RuhunaHapugalaGalleSri Lanka

The necessity to understand the relationship between cyanobacterial species abundance and water quality variations in coastal lagoons is crucial to develop strategies to prevent further cyanobacterial *** paper evaluates the relationship between water quality variations on the distribution of cyanobacteria during a 12-month period in Batticaloa Lagoon（Sri Lanka） using Redundancy analysis and Pearson *** variations in p H,temperature,salinity,dissolved oxygen（DO） and total phosphorus（TP）levels were reported,but not turbidity and NO3-.This brackish waterbody is hypereutrophic（TP levels 〉 0.1 mg/L）.The cyanobacterial community contained 13 genera and 22 ***3-,TP and turbidity levels positively influenced cyanobacterial abundance during all seasons indicating that nutrient（largely phosphorus） and sediment entry control is highly crucial along with periodic monitoring of cyanobacterial growth.

关键词： Batticaloa Lagoon Cyanobacteria Nitrates(NO_3^-) Turbidity Total phosphorous(TP) Water quality

Morphological characterization, antioxidant capacity and diversity of Syzygium cumini trees from Sri Lanka

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Horticultural Plant journal 2022年第1期8卷 53-67页

作者： Prasajith Kapila Dissanayake Wekumbure Gedara Chandima Wekumbura Alge Watage wijeratne Douglas Siril Abeywickrama Wijesundara Faculty of Agricultural Sciences Sabaragamuwa University of Sri LankaBelihuloya 70140Sri Lanka Department of Agronomy Throckmorton Plant Sciences CenterKansas State UniversityManhattanKS 66506USA National Institute of Fundamental Studies Kandy 20000Sri Lanka

Syzygium cumini(L.)Skeels(Madan)is widely distributed in South Asian countries,including Sri Lanka,where it was naturally *** were only few studies reported on *** for its values and other benefits in Sri *** study focuses on morphological characterization and the diversity of *** trees in seven locations of Sri *** were collected on different morphological ***,flower and fruit morphology showed significant differences among ***,trees from Batticaloa region showed different morphology,which would potentially open a different avenue for further study to trace different cultivars across the *** of the diversity index and multivariate analyses indicated high diversity in morphology and antioxidant capacity of *** variables such as leaf area,inter-nodal length,petiole length,anther length,filament length,fruit size and pericarp thickness could be suitable candidates to investigate the morphological variation of *** any *** capacity studies also showed significant differences among locations and *** hue angle was positively correlated to antioxidant *** of this study concluded that the wide distribution of *** trees throughout Sri Lanka in different geographical locations showed high diversity in leaf,flower and fruit morphology together with antioxidant capacity.

关键词： Syzygium cumini Morphological diversity Antioxidant capacity Sri Lanka

Using electronic medical record data to assess chronic kidney disease,type 2 diabetes and cardiovascular disease testing,recognition and management as documented in Australian general practice:a cross-sectional analysis

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Family Medicine and Community Health 2022年第1期10卷 25-35页

作者： julia L jones Natalie G Lumsden Koen Simons Anis Ta'eed Maximilian P de Courten Tissa wijeratne Nicholas Cox Christopher j A Neil jo-Anne Manski-Nankervis Peter Shane Hamblin Edward D janus Craig L Nelson Nephrology Western HealthMelbourneVictoriaAustralia Medicine University of MelbourneMelbourneVictoriaAustralia Western Health Chronic Disease Alliance MelbourneVictoriaAustralia General Practice University of MelbourneMelbourneVictoriaAustralia Epidemiology and Biostatistics University of MelbourneMelbourneVictoriaAustralia Mitchell Institute for Education and Health Policy MelbourneVictoriaAustralia Chronic Disease Prevention and Management Victoria UniversityMelbourneVictoria3011 La Trobe University MelbourneVictoriaAustralia Neurology Western HealthMelbourneVictoriaAustralia Cardiology Western HealthMelbourneVictoriaAustralia Endocrinology and Diabetes Western HealthMelbourneVictoriaAustralia Medicine Western HealthMelbourneVictoriaAustralia

Objectives To evaluate the capacity of general practice(GP)electronic medical record(EMR)data to assess risk factor detection,disease diagnostic testing,diagnosis,monitoring and pharmacotherapy for the interrelated chronic vascular diseases-chronic kidney disease(CKD),type 2 diabetes(T2D)and cardiovascular *** Cross-sectional analysis of data extracted on a single date for each practice between 12 April 2017 and 18 April 2017 incorporating data from any time on or before data extraction,using baseline data from the Chronic Disease early detection and Improved Management in PrimAry Care *** data were extracted from GP EMRs using the Pen Computer Systems Clinical Audit Tool and descriptive statistics used to describe the study *** Eight GPs in Victoria,*** Patients were≥18 years and attended GP≥3 times within 24 months.37946 patients were *** Risk factor and disease testing/monitoring/treatment were assessed as per Australian guidelines(or US guidelines if none available),with guidelines simplified due to limitations in data availability where *** factor assessment in those requiring it:30%of patients had body mass index and 46%blood pressure within guideline recommended *** testing in at-risk population:17%had diagnostic testing as per recommendations for CKD and 37%for *** undiagnosed disease:Pathology tests indicating possible disease with no diagnosis already coded were present in 6.7%for CKD,1.6%for T2D and 0.33%familial *** prevalence:Coded diagnoses were recorded in 3.8%for CKD,6.6%for T2D,4.2%for ischaemic heart disease,1%for heart failure,1.7%for ischaemic stroke,0.46%for peripheral vascular disease,0.06%for familial hypercholesterolaemia and 2%for atrial *** prescriptions:the proportion of patients prescribed guideline-recommended medications ranged from 44%(beta blockers for patients for some condi

关键词： diagnosis testing record