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Effective Isolation of Retrotransposons and Repetitive DNA Families from the Wheat Genome

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Journal of Integrative Plant Biology 2010年第7期52卷 679-691页

作者： Motonori Tomita Munenori asao Aya Kuraki Molecular Genetics Laboratory Faculty of AgricultureTottori University

New classes of repetitive DNA elements were effectively identified by isolating small fragments of the elements from the wheat genome. A wheat A genome library was constructed from Triticum monococcum by degenerate cleavage with EcoO1091, the recognition sites of which consisted of 5＇-PuGGNCCPy-3＇ multi-sequences. Three novel repetitive sequences pTm6, pTm69 and pTm58 derived from the A genome were screened and tested for high copy number using a blotting approach, pTm6 showed identity with integrase domains of the barley Tyl-Copia-retrotransposon BARE-1 and pTm58 showed similarity to the barley Ty3-gypsy-like retrotransposon Romani. pTm69, however, constituted a tandem array with useful genomic specificities, but did not share any identity with known repetitive elements. This study also sought to isolate wheat D-genome-specific repetitive elements regardless of the level of methylation, by genomic subtraction. Total genomic DNA of Aegilops tauschii was cleaved into short fragments with a methylation-insensitive 4 bp cutter, Mbol, and then common DNA sequences between Ae. tauschii and Triticum turgidum were subtracted by annealing with excess T. turgidum genomic DNA. The D genome repetitive sequence pAt1 was isolated and used to identify an additional novel repetitive sequence family from wheat bacterial artificial chromosomes with a size range of 1 395-1 850 bp. The methods successfully led pathfinding of two unique repetitive families.

关键词： DNA Effective Isolation of Retrotransposons and Repetitive DNA Families from the Wheat Genome AABB

Moving computational multi-domain method for modelling the flow interaction of multiple moving objects

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Advances in Aerodynamics 2022年第1期4卷 78-95页

作者： Momoha Nishimura Masashi Yamakawa Shinichi asao Seiichi Takeuchi Mehdi Badri Ghomizad Department of Mechanical and System Engineering Kyoto Institute of TechnologyKyotoJapan Department of Mechanical Engineering College of Industrial TechnologyHyogoJapan

This study proposes a method for modelling the flow interaction of multiple moving objects where the flow field variables are communicated between multiple separate moving computational *** of using the conventional approach with a single fixed computational domain covering the whole flow field,this method advances the moving computational domain(MCD)method in which the computational domain itself moves in line with the motions of an object *** computational domains created around each object move independently,and the flow fields of each domain interact where the flows *** eliminates the spatial restriction for simulating multiple moving ***,a shock tube test verifies that the overset implementation and grid movement do not adversely affect the results and that there is communication between the grids.A second test case is conducted in which two spheres are crossed,and the forces exerted on one object due to the other’s crossing at a short distance are *** results verify the reliability of this method and show that it is applicable to the flow interaction of multiple moving objects.

关键词： Crossing Moving boundary problems Compressible flow Moving mesh method

Flight simulation from takeoff to yawing of eVTOL airplane with coaxial propellers by fluid-rigid body interaction

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Advances in Aerodynamics 2023年第1期5卷 29-43页

作者： Ritsuka Gomi Ayato Takii Masashi Yamakawa Shinichi asao Seiichi Takeuchi Momoha Nishimura Department of Mechanical Engineering Kyoto Institute of TechnologyKyotoJapan Department of Mechanical Engineering College of Industrial TechnologyHyogoJapan

In this study,we adopt a coupled fluid-rigid body simulation using the moving computational domain method and multi-axis sliding mesh method for the takeoff,hovering,and yawing flight of an electric vertical takeoff and landing aircraft(eVTOL).The aircraft model has four pairs of coaxial propellers,and the computational domain is divided into three domains to move the aircraft and eight propeller domains to rotate the *** a result,we clarify the behavior and aerodynamic force of the aircraft when the input values are determined by the automatic *** results in the flow field also show that the downwash spreads in a crisscross pattern on the ground,the wind reaches different ranges on the ground depending on the flight altitude,and that the coaxial propeller causes an asymmetry in the velocity field during ***-quently,we conclude that this method is effective for the flight simulation of an eVTOL.

关键词： Computational fluid dynamics Moving grid Coupled simulation Flying car eVTOL

Extracapsular invasion as a risk factor for disease recurrence in colorectal cancer

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World Journal of Gastroenterology 2011年第15期17卷 2003-2006页

作者： Takaaki Fujii Yuichi Tabe Reina Yajima Satoru Yamaguchi Soichi Tsutsumi Takayuki asao Hiroyuki Kuwano Depart ment of General Surgical Science Graduate School of Medicine Gunma University Gunma 371-8511 Japan

AIM: To evaluate the presence of extracapsular invasion (ECI) in positive nodes as a predictor of disease recurrence disease in colorectal cancer. METHODS: Two hundred and twenty-eight consecutive patients who underwent colorectal resection were identified for inclusion in this study, of which 46 had positive lymph nodes. Among 46 cases with stage Ⅲcolorectal cancer, 16 had ECI at positive nodes and 8 had disease recurrence. The clinical and pathological features of these cases were reviewed. RESULTS: In the univariate analysis, the number of positive lymph nodes and depth of tumor invasion were significantly associated with the presence of ECI at positive nodes. Multivariate analysis demonstrated that only ECI was a predictor of recurrence. The recurrence-free interval differed significantly among patients with ECI at positive ***: Our results suggest that ECI at metastatic nodes can identify which cases are at high risk of short-term disease recurrence in colorectal cancer.

关键词： Extracapsular invasion Lymph node Metastasis Colorectal cancer Risk factor Adjuvant therapy

Mechanism of gastrointestinal abnormal motor activity induced by cisplatin in conscious dogs

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World Journal of Gastroenterology 2014年第42期20卷 15691-15702页

作者： Hiroyuki Ando Erito Mochiki Tetsuro Ohno Mitsuhiro Yanai Yoshitaka Toyomasu Kyoichi Ogata Yuichi Tabe Ryuusuke Aihara Toshihiro Nakabayashi Takayuki asao Hiroyuki Kuwano Department of General Surgical Science Gunma University Graduate School of Medicine

AIM: To investigate whether 5-hydroxytryptamine (serotonin; 5-HT) is involved in mediating abnormal motor activity in dogs after cisplatin administration.

关键词： Cisplatin Emesis 5-hydroxytryptamine Abnormal motor activity Dog

Winning speeches of the 39th Chinese Speech Contest of All Japan

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Voice of Friendship 2022年第1期 46-49页

作者： Urara asao Yuka Matsuki Miyu Minegishi 不详

On Jan 9,2022,the 39th Chinese Speech Contest of All Japan,hosted by the Japan-China Friendship Association,was successfully *** intense competition in the preliminaries and finals,19 candidates won prizes in the high school,social and college *** asao(college group),Yuka Matsuki(social group)and Miyu Minegishi(high school group)won top prizes and were awarded the China-Japan Friendship Association Medals.

关键词： Japan college prize

Assessment of Non-Contact Measurement Using a Microwave Sensor to Jugular Venous Pulse Monitoring

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Journal of Biomedical Science and Engineering 2021年第3期14卷 94-102页

作者： Satoshi Suzuki Masaaki Hoshiga Kentaro Kotani Takafumi asao Faculty of Engineering Science Kansai University Osaka Japan Faculty of Medicine Osaka Medical College Osaka Japan

The jugular venous pulse (JVP) waveform provides an insight into right heart function, and its assessment is important in patients with heart failure. However, the conventional pulse-transducer (contact) method for monitoring this waveform is not frequently used because it requires a high degree of skill. The aim of this study was to confirm the effectiveness of a prototype non-contact system that employs microwave radar (24 GHz, 7 mW;non-contact system) for JVP measurement. Experiments were conducted on eight healthy male volunteers (21.88 ± 0.99 years). JVP measurements were compared between the conventional contact method and the proposed non-contact method. Change in JVP waveform was measured in response to an angle of reclining in five steps from the supine position to 75° of elevation. The obtained JVP measurements were similar between the two methods. Because in the non-contact method the faint pulsation of the JVP is not suppressed by the pressure of a sensor placed on the skin, the prototype microwave radar system is particularly suitable for evaluating the JVP waveform.

关键词： Microwave Radar Non-Contact Monitoring Jugular Venous Pressure Right Heart Function

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World Journal of Gastroenterology 2006年第11期12卷 1805-1809页

作者： Yoshimi Nakajima Hitoshi Takagi Naondo Sohara Ken Sato Satoru Kakizaki Kenichi Nomoto Hideki Suzuki Taketoshi Suehiro Tatsuo Shimura Takayuki asao Hiroyuki Kuwano Masatomo Mori Ken Nishikura Department of Medicine and Molecular Science Gunma University Graduate School of Medicine MaebashiGunma 371-8511Japan Department of Surgery Gunma University Graduate School of Medicine MaebashiGunma 371-8511Japan Division of Molecular and Functional Pathology Department of Cellular Function Niigata University Graduate School of Medicine and Dental SciencesNiigataNiigata 951-8510Japan

A 42-year-old woman was admitted to our hospital because of multiple liver tumors detected by ultrasonography. Colonoscopy revealed submucosal tumor in the rectum, which was considered the primary lesion. Endoscopic mucosal resection followed by histopathological examination revealed that the tumor was carcinoid. The resected margin of the tumor was positive for malignant cells. Two courses to transcatheter arterial chemotherapy for liver metastasis were ineffective. Accordingly, the rectal tumor and metastatic lymph nodes were surgically resected. One month after the operation, she received liver transplantation （left lateral segment and caudate lobe） from her son. No recurrent lesion has been observed at two years after the liver transplantation. Liver transplantation should be considered as a treatment option even in advanced case of carcinoid metastasis to the liver. We also discuss the literature on liver transplantation for metastatic carcinoid tumor.

关键词： Carcinoid Liver metastasis Living related liver transplantation

An investigation using high-precision CCD laser displacement sensor to measure body surface motion induced by heartbeat

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Journal of Biomedical Science and Engineering 2012年第11期5卷 672-677页

作者： Satoshi Suzuki Takemi Matsui Takafumi asao Kentaro Kotani Department of Management Systems Engineering Faculty of System Design Tokyo Metropolitan University Tokyo Japan Department of Mechanical Engineering Faculty of Engineering Science Kansai University Osaka Japan

The aim of this study was to measure the motion of the body surface induced by heartbeat by using a high-precision laser sensor in order to provide a reference for other research on medical remote sensing techniques that are unconstrained and non-contact. Body surface motions at some points on the chest and on the dorsal region were measured for eight men with a laser displacement sensor. For comparison, an electrocardiograph was also used to monitor the heartbeat. As a result, we confirmed cyclic motion of about 0.1 mm in amplitude everywhere on the body surface, not only from the front of the body but also from the back and the neck. In contrast, which part on the body was most suitable for measurement had to be taken into account because a difference in motion amplitudes was observed in different parts on the body. The amplitude of the motions also depended on the conditions of the body surface as well as underneath the skin. These results show the possibility of acquiring information about heartbeats from anywhere on the body by using an unconscious sensing technique, and the potential for the technique to monitor the condition of the body and personal physical characteristics.

关键词： Unconstrained Sensing Heart Rate Body Surface Motion