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Gut microbiota predicts the diagnosis of celiac disease in Saudi children

World Journal of Gastroenterology 2023年第13期29卷 1994-2000页

作者： Mohammad El Mouzan asaad assiri Ahmed Al Sarkhy Department of Pediatrics(Gastroenterology Unit) King Saud UniversityRiyadh 11461Saudi Arabia

BACKGROUND Celiac disease(CeD)is a multisystem immune-mediated multifactorial condition strongly associated with the intestinal *** To evaluate the predictive power of the gut microbiota in the diagnosis of CeD and to search for important taxa that may help to distinguish CeD patients from *** Microbial DNA from bacteria,viruses,and fungi,was isolated from mucosal and fecal samples of 40 children with CeD and 39 *** samples were sequenced using the HiSeq platform,the data were analyzed,and abundance and diversities were *** this analysis,the predictive power of the microbiota was evaluated by calculating the area under the curve(AUC)using data for the entire *** Kruskal-Wallis test was used to evaluate the significance of the difference between *** Boruta logarithm,a wrapper built around the random forest classification algorithm,was used to identify important bacterial biomarkers for *** In fecal samples,AUCs for bacterial,viral,and fungal microbiota were 52%,58%,and 67.7%respectively,suggesting weak performance in predicting ***,the combination of fecal bacteria and viruses showed a higher AUC of 81.8%,indicating stronger predictive power in the diagnosis of *** mucosal samples,AUCs for bacterial,viral,and fungal microbiota were 81.2%,58.6%,and 35%,respectively,indicating that mucosal bacteria alone had the highest predictive *** bacteria,Bacteroides intestinalis and Burkholderiales bacterium 1-1-47,in fecal samples and one virus,Human_endogenous_retrovirus_K,in mucosal samples are predicted to be“important”biomarkers,differentiating celiac from nonceliac disease *** intestinalis is known to degrade complex arabinoxylans and xylan which have a protective role in the intestinal ***,several Burkholderiales species have been reported to produce peptidases that hydrolyze gluten peptides,with the potential to reduce the gluten content of ***,a role fo

关键词： Celiac disease Microbial signature Children Saudi Arabia

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Gut microbiota predicts the diagnosis of ulcerative colitis in Saudi children

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World Journal of Clinical Pediatrics 2024年第1期13卷 118-124页

作者： Mohammad El Mouzan Ahmed Al Sarkhy asaad assiri Department of Pediatrics Gastroenterology UnitKing Saud UniversityRiyadh 11461Saudi Arabia

BACKGROUND Ulcerative colitis(UC)is an immune-mediated chronic inflammatory condition with a worldwide *** the etiology of this disease is still unknown,the understanding of the role of the microbiota is becoming increasingly *** To investigate the predictive power of the gut microbiota for the diagnosis of UC in a cohort of newly diagnosed treatment-naïve Saudi children with *** The study population included 20 children with a confirmed diagnosis of UC and 20 healthy *** DNA was extracted and sequenced,and shotgun metagenomic analysis was performed for bacteria and *** and bioinformatics demonstrated significant dysbiosis in the form of reduced alpha diversity,beta diversity,and significant difference of abundance of taxa between children with UC and control *** receiver operating characteristic curve,a probability curve,was used to determine the difference between the UC and control *** area under the curve(AUC)represents the degree of separability between the UC group and the control *** AUC was calculated for all identified bacterial species and for bacterial species identified by the random forest classification algorithm as important potential biomarkers of UC.A similar method of AUC calculation for all bacteriophages and important species was *** The median age and range were 14(0.5-21)and 12.9(6.8-16.3)years for children with UC and controls,respectively,and 40%and 35%were male for children with UC and controls,*** AUC for all identified bacterial species was 89.5%.However,when using the bacterial species identified as important by random forest classification algorithm analysis, the accuracy increased to 97.6%. Similarly, the AUC for all theidentified bacteriophages was 87.4%, but this value increased to 94.5% when the important bacteriophagebiomarkers were *** very high to excellent AUCs of fecal bacterial and viral species sugge

关键词： Ulcerative colitis Microbiota Area under the curve Children Saudi Arabia

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Regional variation of pediatric inflammatory bowel disease in Saudi Arabia: Results from a multicenter study

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World Journal of Gastroenterology 2020年第4期26卷 416-423页

作者： Mohammad I El Mouzan Mohammed H AlEdreesi Mohammed Y Hasosah Abdulrahman A Al-Hussaini Ahmad A Al Sarkhy asaad a assiri Department of Pediatrics Gastroenterology UnitKing Saud UniversityRiyadh11461Saudi Arabia Specialty Pediatrics Division Pediatric GastroenterologyJohns Hopkins Aramco HealthcareDhahran 31311Saudi Arabia Department of Pediatrics Gastroenterology UnitKing Saud bin Abdulaziz University for Health SciencesJeddah 21423Saudi Arabia Children Hospital Gastroenterology DivisionKing Fahad Medical CityRiyadh 11525Saudi Arabia Supervisor of Prince Abdullah Bin Khalid Celiac Disease Research Chair Gastroenterology UnitKing Saud UniversityRiyadh 11461Saudi Arabia

BACKGROUND Incidence and severity variations of inflammatory bowel disease(IBD)have been reported from Western populations between continents and regions of the same ***,no data were available from other *** To investigate the regional differences in the IBD profiles of pediatric patients from the Kingdom of Saudi *** Data from a national multicenter IBD study were *** incidence,time trend,and clinical presentation of Crohn’s disease(CD)and ulcerative colitis(UC)in the Central region(CR),Western region(WR),and Eastern region(ER)were analyzed and *** analysis included Poisson regression analysis for incidence variation and Chi-square test for demographic and clinical parameters.A P<0.05 was considered *** The prevalence of positive family history was lower in children with CD from the ER than the CR or the *** rate was higher in children with CD and UC from the CR and the ER,*** incidences and time trends of CD and UC were not significantly different between *** the ER,a significantly higher percentage of children with CD presented with abdominal pain(P<0.001),blood in stools(P=0.048),stricturing or penetrating disease(P=0.029),higher erythrocyte sedimentation rate(P<0.001),higher C-reactive protein(P<0.001),higher anemia(P=0.017),and lower albumin level(P=0.014).For children with UC from the ER,a significantly higher percentage presented with anemia(P=0.006)and a lower percentage with pancolitis(P<0.001).CONCLUSION The most important finding is the identification of significantly more severe presentation of CD in the ER of the Kingdom of Saudi *** studies are needed to explain such variations.

关键词： Crohn’s disease Ulcerative colitis Epidemiology Children Regional variation

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Fungal dysbiosis predicts the diagnosis of pediatric Crohn's disease

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World Journal of Gastroenterology 2018年第39期24卷 4510-4516页

作者： Mohammad I El Mouzan Kirill S Korolev Mohammad A Al Mofarreh Rajita Menon Harland S Winter Ahmad A Al Sarkhy Scot E Dowd Ahmad M Al Barrag asaad a assiri Department of Pediatrics King Saud UniversityRiyadh 11461Saudi Arabia Department of Pediatrics Supervisor of Prince Abdullah Bin Khalid Celiac Disease Research ChairKing Saud UniversityRiyadh 11461Saudi Arabia Bioinformatics Program Boston UniversityBostonMA 02215United States Al Mofarreh PolyClinic Riyadh 11423Saudi Arabia MassGeneral Hospital for Children BostonMA 02114United States MRDNA ShallowaterTX 79363United States Department of Microbiology King Saud UniversityRiyadh 11461Saudi Arabia

AIM To investigate the accuracy of fungal dysbiosis inmucosa and stool for predicting the diagnosis of Crohn's disease(CD). METHODS Children were prospectively enrolled in two medical centers: one university hospital and one private gastroenterology clinic in the city of Riyadh, Kingdom of Saudi Arabia. The children with confirmed diagnosis of CD by standard guidelines were considered cases, and the others were considered non-inflammatory bowel disease controls. Mucosal and stool samples were sequenced utilizing Illumina MiSeq chemistry following the manufacturer's protocols, and abundance and diversity of fungal taxa in mucosa and stool were analyzed. Sparse logistic regression was used to predict the diagnosis of CD. The accuracy of the classifier was tested by computing the receiver operating characteristic curves with 5-fold stratified cross-validation under 100 permutations of the training data partition and the mean area under the curve(AUC) was calculated. RESULTS All the children were Saudi nationals. There were 15 children with CD and 20 controls. The mean age was 13.9(range: 6.7-17.8) years for CD children and 13.9(3.25-18.6) years for controls, and 10/15(67%) of the CD and 13/20(65%) of the control subjects were boys. CD locations at diagnosis were ileal(L1) in 4 and colonic(L3) in 11 children, while CD behavior was non-stricturing and non-penetrating(B1) in 12 and stricturing(B2) in 3 children. The mean AUC for the fungal dysbiosis classifier was significantly higher in stools(AUC = 0.85 ± 0.057) than in mucosa(AUC = 0.71 ± 0.067)(P < 0.001). Most fungal species were significantly more depleted in stools than mucosal samples, except for Saccharomyces cerevisiae and S. bayanus, which were significantly more abundant. Diversity was significantly more reduced in stools than in mucosa. CONCLUSION We found high AUC of fungal dysbiosis in fecal samples of children with CD, suggesting high accuracy in predicting diagnosis of CD.

关键词： Fungiome Mycobiome Crohn’s disease Inflammation Saudi children

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