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Testing the effect of individual color morphology on immune response in bush-crickets

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Insect Science 2010年第5期17卷 400-405页

作者： asa Berggren Department of Ecology Swedish University of Agricultural Sciences Uppsala Sweden

Despite the growing interest in how an individual＇s immune response is correlated to morphological and ecological factors, little empirical data has been available from wild insect populations. Many insects have different color morphs and exhibit differences in immune responses. Links are expected to exist between body colors and immune function in insects, because the same biochemical precursors involved in the immune response are responsible for melanin-based color markings. In this study, I assay the immune response of two different color morphs of 377 wild-caught bush-crickets, Metrioptera roeseli, from 20 populations by measuring individual encapsulation responses to a surgically implanted nylon monofilament. There was no difference between green and brown bush-cricket morphs （low melanin vs high melanin investment in cuticula color respectively） and their ability to mount an immune response to the implant. Further study is needed on the relationship between color morphology and immune response in wild insects, and whether trade-offs occur between factors during an insect＇s development phase and long-term health.

关键词： color morph cuticula experiment immune response melanin parasite

Surrogate potency assays:Comparison of binding profiles complements dose response curves for unambiguous assessment of relative potencies

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Journal of Pharmaceutical Analysis 2018年第2期8卷 138-146页

作者： Robert Karlsson Veronica Fridh asa Frostell Purification and Analysis GE Healthcare Life SciencesUppsalaSweden

Surface plasmon resonance(SPR) systems are widely used for detailed characterization of antibody activities including antigen and Fc-receptor binding. During the later stages of development, where the focus is to ensure that established critical quality attributes(CQAs) are maintained during cell culture,purification and formulation processes, analysis is simplified, and relative potencies are often determined. Here, simulation of binding data revealed that relative potency values, determined via parallel line analysis(PLA) and half maximal effective concentration(EC50) analysis accurately reflect changes in active concentration only if binding kinetics remain unchanged. Changes in the association rate constant shifted dose response curves, and therefore relative potencies, in the same way as changes in analyte concentration do. However, for interactions characterized by stable binding, changes in the dissociation rate constant did not result in any shift, suggesting that this type of change may go unnoticed in the dose response curve. Thus, EC50 and PLA analyses of dose response curves obtained with an anti-TNF-α antibody were complemented with the Biacore functionality for sensorgram comparison analysis, whereby changes in antigen and Fc-receptor binding profiles could be detected. Next, analysis of temperature stressed TNF-α antibody revealed that calibration free concentration analysis(CFCA) data correlated perfectly with relative potency values. Together, these results demonstrate that combinations of SPR based dose response curves, sensorgram comparison and CFCA can be used to strengthen the confidence in relative potency assessments, and suggest that SPR can potentially be used as a surrogate potency assay in the quality control of biotherapeutic medicines.

关键词： Surface plasmon resonance EC50 Sensorgram comparison Calibration free concentration analysis Surrogate potency assay TNF-a

Quantification and depth distribution analysis of carbon to nitrogen ratio in forest soils using reflectance spectroscopy

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International Soil and Water Conservation Research 2023年第1期11卷 112-124页

作者： asa Gholizadeh Mohammadmehdi Saberioon Nastaran Pouladi Eyal Ben-Dor Department of Soil Science and Soil Protection Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences Prague16500PragueCzech Republic Section 1.4 Remote Sensing and Geoinformatics Helmholtz Centre Potsdam GFZ German Research Centre for GeosciencesTelegrafenberg14473Potsdam Germany knoell Germany GmbH Konrad-Zuse-Ring68163MannheimGermany Remote Sensing Laboratoryeparmentof Ggraphy and HumanEnvionmnt Porter Schoolf Evionmntand Earth ScienceTelAvivUniverty 69978TelAvivIsrael

Forest soils have large contents of carbon(C)and total nitrogen(TN),which have significant spatial variability laterally across landscapes and vertically with depth due to decomposition,erosion and ***,the ratio of C to TN contents(C∶N),a crucial indicator of soil quality and health,is also different depending on soil *** attributes can cost-effectively and rapidly be estimated using visible-near infrared-shortwave infrared(VNIR-SWIR)***,the effect of different soil layers,particularly over large scales of highly heterogeneous forest soils,on the perfor-mance of the technique has rarely been *** study evaluated the potential of VNIR-SWIR spectroscopy in quantification and variability analysis of C∶N in soils from different organic and min-eral layers of forested sites of the Czech *** each site,we collected samples from the litter(L),fragmented(F)and humus(H)organic layers,and from the A_(1)(depth of 2-10 cm)and A_(2)(depth of 10-40 cm)mineral layers providing a total of 2505 *** vector machine regression(SVMR)was used to train the prediction models of the selected attributes at each individual soil layer and the merged layer(profile).We further produced the spatial distribution maps of C∶N as the target attribute at each soil *** showed that the prediction accuracy based on the profile spectral data was adequate for all ***,F was the most accurately predicted layer,regardless of the soil attribute.C∶N models and maps in the organic layers performed well although in mineral layers,models were poor and maps were reliable only in areas with low and moderate C∶*** the other hand,the study indicated that reflectance spectra could efficiently predict and map organic layers of the forested ***,in mineral layers,high values of C∶N(≥50)were not detectable in the map created based on the reflectance *** general,the study suggests that VNIR-SWIR spectrosc

关键词： Forest soil Soilorganiccarbon C:N Soilhorizons VNIR-SWIRspectroscopy

Water-saving irrigation of paddy field to reduce nutrient runoff

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Journal of Environmental Sciences 2010年第6期22卷 885-891页

作者： Tadayoshi Hitomi Yusaku Iwamoto asa Miura Koji Hamada Kyoji Takaki Eisaku Shiratani National Institute for Rural Engineering Kyusyu Regional Agricultural Administration Office

The purpose of this work is to study the effect of a type of water-saving irrigation （WSI） on nutrient runoff of paddy field. The volume of surface drainage was maintained low by WSI. In particular, WSI effectively reduced surface drainage in rain events. Model simulation indicated that net runoff load of total nitrogen （TN） from the paddy field was increased by WSI. Meanwhile, net runoff loads of total phosphorus （TP） and total organic carbon （TOC） from the paddy field was decreased by WSI. Because ponding waters of the study fields were enriched with TP and TOC, WSI reduced runoff of these nutrients by controlling the volume of surface drainage. WSI could be considered an efficient method for reducing runoff loads and could conserve water quality in an agricultural watershed.

关键词： model simulation net runoff load storage capacity surface drainage tank model water management

抽水蓄能电站压力钢管内外壁自动化同步除锈技术浅析

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水电站机电技术 2024年第5期47卷 73-74页

作者：邱母阿萨子张文明中国水利水电第七工程局有限公司机电安装分局四川彭山620860

近些年随着国内抽水蓄能电站建设的不断发展,对抽水蓄能电站建设的质量要求也越来越高,其中,压力钢管除锈及防腐为钢管制作的最后一个工序,其技术质量至关重要,而往往影响钢管防腐质量的重要因素为钢管除锈工艺技术不够高,从而导致防腐... 详细信息

近些年随着国内抽水蓄能电站建设的不断发展,对抽水蓄能电站建设的质量要求也越来越高,其中,压力钢管除锈及防腐为钢管制作的最后一个工序,其技术质量至关重要,而往往影响钢管防腐质量的重要因素为钢管除锈工艺技术不够高,从而导致防腐的整体质量不达标,影响压力钢管的整体质量及使用寿命。本文重点对压力钢管内外壁自动化除锈的关健技术进行分析,提出了控制压力钢管内外壁同步除锈的质量,从而提高压力钢管制作速度及防腐的整体质量。

关键词：压力钢管自动化同步除锈关键技术

抽水蓄能电站压力钢管加劲环自动化焊接技术研究与应用

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水电站机电技术 2024年第7期47卷 82-83,119页

作者：张文明邱母阿萨子中国水利水电第七工程局有限公司机电安装分局四川彭山620860

随着国内抽水蓄能电站迅猛发展,引水系统隧洞大批量采用压力钢管,怎样让压力钢管安全高效的生产和安装成了难题。本文主要从压力钢管加劲环下料、制作与安装、焊接方法选择、工艺参数选择等方面阐述了压力钢管在水平状态下,采用埋弧焊... 详细信息

随着国内抽水蓄能电站迅猛发展,引水系统隧洞大批量采用压力钢管,怎样让压力钢管安全高效的生产和安装成了难题。本文主要从压力钢管加劲环下料、制作与安装、焊接方法选择、工艺参数选择等方面阐述了压力钢管在水平状态下,采用埋弧焊技术焊接加劲环角焊缝,为同类型压力钢管加劲环安装焊接工序提供了一种有效的方法。

关键词：压力钢管加劲环焊接自动化焊接技术

Leaf chlorophyll and nitrogen dynamics and their relationship to lowland rice yield for site-specific paddy management

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Information Processing in Agriculture 2017年第4期4卷 259-268页

作者： asa Gholizadeh Mohammadmehdi Saberioon Lubos Boruvka Aimrun Wayayok Mohd Amin Mohd Soom Department of Soil Science and Soil Protection Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences PraguePragueCzech Republic Laboratory of Signal and Image Processing Institute of Complex SystemsSouth Bohemia Research Centre of Aquaculture and Biodiversity of HydrocenosesFaculty of Fisheries and Protection of WatersUniversity of South Bohemia in Ceske BudejoviceNove HradyCzech Republic Department of Biological and Agricultural Engineering Faculty of EngineeringUniversity Putra MalaysiaSelangorMalaysia Faculty of Sustainable Agriculture University Malaysia SabahSandakanMalaysia

The optimum rate and application timing of Nitrogen(N)fertilizer are crucial in achieving a high yield in rice cultivation;however,conventional laboratory testing of plant nutrients is time-consuming and *** develop a site-specific spatial variable rate application method to overcome the limitations of traditional techniques,especially in fields under a double-cropping system,this study focused on the relationship between Soil Plant Analysis Development(SPAD)chlorophyll meter readings and N content in leaves during different growth stages to introduce the most suitable stage for assessment of crop N and prediction of rice *** SPAD readings and leaf N content were measured on the uppermost fully expanded leaf at panicle formation and booting *** yield was also measured at the end of the *** analysis of variance,variogram,and kriging were calculated to determine the variability of attributes and their relationship,and finally,variability maps were *** linear relationships were observed between attributes,with the same trends in different sampling dates;however,accuracy of semivariance estimation reduces with the growth *** of the study also implied that there was a better relationship between rice leaf N content(R^2=0.93),as well as yield(R2=0.81),with SPAD readings at the panicle formation ***,the SPAD-based evaluation of N status and prediction of rice yield is more reliable on this stage rather than at the booting *** study proved that the application of SPAD chlorophyll meter paves the way for real-time paddy N management and grain yield *** can be reliably exploited in precision agriculture of paddy fields under double-cropping cultivation to understand and control spatial variations.

关键词： Spatial variability Non-invasive measurement Precision farming Decision support

Polymorphisms in sulfotransferase 1A1 and glutathione 5-transferase P1 genes in relation to colorectal cancer risk and patients' survival

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World Journal of Gastroenterology 2005年第43期11卷 6875-6879页

作者： Xiao-Feng Sun Ahmad Ahmadi Gunnar Arbman asa Wallin Daniel Asklid Hong Zhang Department of Oncology Institute of Biomedicine and Surgery University of Linkoping Linkoping Sweden Daniel Asklid Hong Zhang Department of Cell Biology Institute of Biomedicine and Surgery University of Linkoping Linkoping Sweden Department of Surgery Vrinnevi Hospital Norrkoping Sweden

AIM： To examine whether polymorphisms in SULTIA1 and GSTP1 genes contribute to colorectal cancer development and whether they are associated with clinicopathological variables are not well identified. METHODS： We examined the genotypes of 125 colorectal cancer patients and 666 healthy controls in a Swedish population by using PCR-restriction fragment length polymorphism （RFLP）. RESULTS： SULTIA1 ＊2/＊2 genotype （OR = 2.49, 95%CI = 1.48-4.19, P = 0.0002） and ＊2 allele （OR = 1.56, 95%CI =1.16-2.10, P = 0.002） had an effect on colorectal cancer susceptibility, while GSTP1 genotype was without effect. However, GSTP1 G-type predicted a worse prognosis in the patients independently of gender, age, Dukes＇ stage, growth pattern, and differentiation （P = 0.03）.CONCLUSION： Polymorphism in SULTIA1 may predispose to colorectal cancer and GSTP1 may be a biological indicator of prognosis in the patients.

关键词： GSTP1 SULTIA1 Survival Colorectal cancer PCR-RFLP

A comparative analysis of child passenger restraint use in China and the United States

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World Journal of Pediatrics 2017年第6期13卷 593-598页

作者： Jing-Zhen Yang Li-Ping Li Hong-Qian Wu Daniel McGehee Corinne Peek-asa The Research Institute at Nationwide Children's Hospital Center for Injury Research and PolicyUnited States Department of Pediatrics College of Medicinethe Ohio State UniversityUnited States Injury Prevention Research Center Shantou University Medical CollegeChina Department of Biostatistics University of IowaUnited States Injury Prevention Research Center Human Factors and Vehicle Safety Research Division University of Iowa Public Policy CenterUnited States

Background:Few published studies have examined child passenger safety practices across *** study compared the prevalence and associated factors of child passenger restraint use among children,aged 0 to 17 in the state of Iowa in the United States,and the city of Shantou in ***:Child restraint use observations were conducted in Iowa and in Shantou in 2012,respectively,among child *** in Iowa were conducted at randomly selected gas stations,while in Shantou observations were completed at randomly selected schools or medical *** observers approached the driver,observed restraint use,and collected brief survey ***:A total of 3049 children from Iowa and 3333 children aged 0 to 17 years from Shantou were *** children aged 0 to 3 years,only 0.1% were compliantly restrained in Shantou as compared to 95.9% in *** proportion of children who were compliantly restrained in Shantou increased with age,but generally decreased with age in *** Shantou,36.0% of children aged 0 to 3 were sitting in the front seat as compared to only 1.7% of children of the same age in *** seat belt use was significantly associated with child restraint in both Iowa and Shantou;the association was stronger in Iowa than Shantou for aH age ***:A significantly higher prevalence of children who were not appropriately restrained was observed in Shantou than in *** findings support the need of mandatory child safety restraint use legislation in China.

关键词： child passenger safety restraint cross country motor vehicle occupant