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Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts

Journal of Integrative Medicine 2017年第4期15卷 310-319页

作者： Bhanuz Dechayont arunporn itharat Pathompong Phuaklee Jitpisute Chunthorng-Orn Thana Juckmeta Nuntika Prommee Nitra Nuengchamnong Pintusorn Hansakul Department of Applied Thai Traditional Medicine Faculty of Medicine Thammasat University Center of Excellence in Applied Thai Traditional Medicine Research Thammasat University Division of Applied Thai Traditional Medicine Faculty of Public Health Naresuan University Science Laboratory Centre Faculty of Science Naresuan University Department of Preclinical Science Faculty of Medicine Thammasat University

OBJECTIVE： To investigate the antioxidant activities as well as phytochemical constituents of Antidesma thwaitesianum Mull. Arg. leaf extracts. METHODS： The leaves of A. thwaitesianum were extracted using three different methods： blending with distilled water, maceration with ethanol and decoction. The chemical antioxidant activity of the plant leaf extracts was evaluated using 2,2-diphenyl-1-picryhydrazyl （DPPH） radical and 2,2＇-azinobis（3- ethylbenzo-thiazoline-6-sulfonic acid） diammonium salt （ABTS＊） radical scavenging assays, as well as the ferric reducing antioxidant power assay. Cellular antioxidant activity was determined by superoxide and nitric oxide scavenging assays. The cytotoxicity of the leaf extracts in RAW 264.7 and differentiated HL-60 cells was tested in parallel using 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide and 3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium assays, respectively. The total phenolic and flavonoid contents were also assessed by spectrophotometric analysis Phytochemical constituents of the most potent extract were investigated by liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer （LC-ESI-QTOF-MS/MS）. RESULTS： The ethanolic （ME） and decoction （LW） extracts of dried leaves had the highest chemical scavenging activity against DPPH and ABTS＋ free radicals with half maximal effective concentration （EC50） values ranging from 3.54 to 6.44 μg/mL. ME and LW exerted moderate ferric reducing activity, with ferric reducing antioxidant power values of 847.41 and 941.26 mg Fe2＋/g extract, respectively. Similarly, ME showed potent cellular scavenging activity against superoxide and nitric oxide radicals with EC50 values of 58.12 and 71.90 μg/mL, respectively. However, LW exhibited only strong nitric oxide scavenging activity with an EC50 value of 91.20 μg/mL. The cell viability of RAW 264.7 and HL-60 cells was greater than 70% in all tes

关键词： Antidesma thwaitesianum Mull. Arg. antioxidants phytochemicals spectrometry, mass,electrospray ionization chromatography, liquid

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The Variety Selection and Testing for Effect on HepG-2 Cell Line of Dioscorea membranacea Pierre （Hua Khao Yen Tai） Extract Using Molecular Genetics

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Journal of Life Sciences 2014年第4期8卷 322-328页

作者： Nantana Jaengsuwan arunporn itharat Department of Applied Biology Thepsatri Rajabhat University Mueang Lop Buri 15000 Thailand Department of Applied Thai Traditional Medicine Faculty of Medicine Thammasat University Klongluang Patumtani 12120Thailand

In this research, Dioscorea membranacea Pierre was studied by using 30 samples from Khao Ruak Sub-district, Chai Badan District, Lop Buri Province, in Thailand. In this research, some morphology was studied including shapes, leaves, stem colors, epidermal cells, stomata sizes and stipules. To study the genetic relationships, the AFLP technique and computer program were used. The Dioscorea membranacea Pierre was classified into 2 groups according to its phylogenetic type: the first group was "Hua Khao Yen Tai-Nuea" (Smilax corbularia Kunth), and the second group included 30 further samples of Hua Khao Yen Tai (Dioscorea membranacea Pierre). The ethanolic crude extract was also applied to test the anti-proliferative activity in the liver hepatocellular carcinoma (HepG2) cell lines which illustrates the characteristics of apoptosis: cell shrinkage, membrane blebbing and nuclear condensation. The expression of Bax gene is increased more than that of the control group while Bcl-2 gene which is anti-apoptotic is decreased. Furthermore, the result of western blot analysis reveals the up-regulation of Bax protein and down-regulation of Bcl-2 protein when compared with untreated cells. This might indicate that ethanolic crude extracts of Hua Khao Yen Tai could induce apoptosis and anti-proliferative on HepG2 cell lines. The results also revealed that some morphology cannot be used to predict which Dioscorea membranacea Pierre plants would be most effective.

关键词：粗提取物细胞系分子遗传学肝癌薯蓣 TAI Bcl-2基因 Bcl-2蛋白

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