检索结果-南通市图书馆

Affinity peptide developed by phage display selection for targeting gastric cancer

World Journal of Gastroenterology 2012年第17期18卷 2053-2060页

作者： Wen-Jie Zhang Yan-Xia Sui arun budha Jian-Bao Zheng Xue-Jun Sun Ying-Chun Hou Thomas D Wang Shao-Ying Lu Department of General Surgery The First Affiliated Hospital of Medical School Xian JiaotongUniversity Xi＇an 710061 Shaanxi Province China The Third Hospital of Chengdu Chengdu610031 Sichuan Province China Shaanxi Normal University Xi＇an 710061Shaanxi Province China Department of Medicine Division of Gastroenterology and Hepatology School of Medicine Universityof Michigan Ann Arbor MI 48109 United States

AIM:To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric ***:A peptide screen was performed by biopanning the PhD-12 phage display library,clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer *** binding of selected peptides was confirmed by bound phage counts,enzyme-linked immunosorbent assay,competitive inhibition,fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer ***:Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal-appearing gastric *** the third round of positive screening,the peptide sequence AADNAKTKSFPV(AAD) appeared in 25%(12/48) of the analyzed *** the control peptide,these values were 6.8 ± 2.3,5.1 ± 1.7,3.5 ± 2.1,4.6 ± 1.9 and 1.1 ± 0.5,*** values for AAD peptide were statistically signif icant(P < 0.01) for gastric cancer as compared with other histological classif ications and control ***:A novel peptide is discovered to have a specific binding activity to gastric cancer,and can be used to distinguish neoplastic from normal gastric mucosa,demonstrating the potential for early cancer detection on endoscopy.

关键词： Gastric cancer Peptide Phage library Molecular imaging Early detection Immunohistochemistry Enzyme-linked immunosorbent assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：