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Influence of previous plane of nutrition on molecular mechanisms regulating the expression of urea and water metabolism related genes in the rumen and kidney of finishing crossbred Angus steers

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Animal Nutrition 2024年第2期17卷 232-243页

作者： Aghata E.Moreira da Silva arturo macias.franco Bradley S.Ferguson Mozart A.Fonseca College of Agriculture Biotechnology&Natural Resources University of Nevada Department of Animal and Range Sciences Clayton Livestock Research Center New Mexico State University

This study aimed to understand how molecular mechanisms controlling water and urea metabolism at the finishing phase can be affected by previous plane of nutrition of crossbred Angus beef steers. Twentyfour(n = 24) animals were randomly distributed into either a moderate(MP) or high plane of nutrition during the background phase for 85 d. Animals were then blocked by their previous plane and were moved onto a 105-d finishing phase in a 2 × 2 factorial arrangement. The forage-finished group received only high-quality alfalfa hay, whereas the grain-fed group received a high grain diet(80% whole corn and20% alfalfa hay). By the end of the finishing phase, animals were harvested and tissue samples from the rumen and kidney were collected. Changes in gene expression of aquaporins(AQP)-2,-3,-4,-7, ATP1A1,ATP1B1, SGK1, CLIC1(kidney and rumen), UT-A1(kidney only) and UT-B(rumen only), were assayed via real-time q PCR; 18S r RNA was used as an endogenous control. One-way ANOVA followed by Tukey's post hoc analysis was conducted. When animals were from MP, forage-finishing increased the relative abundance of AQP3(P ≤ 0.05), AQP7(P ≤ 0.05), ATP1B1(P ≤ 0.05), and SGK1(P ≤ 0.05) in the kidney when compared to grain-fed animals. In the rumen, for the MP group, AQP7 was differentially expressed in both treatments at the finishing phase(P ≤ 0.01), with forage-finished steers having the highest expression of AQP7. For the MP group, UT-B had a tendency of presenting a higher expression on grainfed animals(P = 0.075). Overall, these results suggest that previous plane can impact expression of genes associated with water and urea metabolism during the finishing phase, namely AQP3, AQP7, ATP1B1, and SGK1 in the kidney, and AQP7 and UT-B in the rumen. The greatest impact observed on gene expression changes of investigated genes at the finishing phase was reflective of animals backgrounded on the moderate previous plane.

关键词： Gene expression Aquaporin Sodium channel Urea transporter

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Effectiveness of Selected Microbial Strains on the Growth of Sweet Sorghum

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Agricultural Sciences 2015年第8期6卷 772-777页

作者： arturo Díaz-franco Oscar Arath Grageda-Cabrera Mónica Guadalupe Lozano-Contreras Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias (INIFAP) Campo Experimental Rio Bravo Km 61 Carretera Matamoros-Reynosa Rio Bravo Tamaulipas Mexico Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias (INIFAP) Campo Experimental Bajio Km 6.5 Carretera Celaya-San Miguel de Allende Celaya Guanajuato Mexico Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias (INIFAP) Campo Experimental Mococha Km 25 Carretera Merida-Motul Yucatan Mexico

Microbial inoculants are of great importance in agro-ecology and plants. However, the symbiotic effectiveness of strains may vary between species or within the same species. Aim of the study was to select experimental microbial strains of national origin, to know its impact on growth of sweet sorghum (Sorghum bicolor) in greenhouse and sterile soil. Five strains of arbuscular mycorrhizal fungi (AMF) and eight rhizobacteria (two of them mutagenized) were compared with non-inoculated plants as control. Variables measured were index of chlorophyll SPAD, plant height, stem diameter (from the middle section), fresh biomass from stem, leaf, panicle, radical, and stem sugars content (°Brix) of every two internodes. The results showed that in the majority of cases, microbial strains exceeded the values of variables compared with control plants. The highest values of chlorophyll (41.8 SPAD), stem diameter (13.5 mm) and total fresh biomass (144.3 g), were recorded with rhizobacterias BS410 (Herbaspirillum sp.), B2709 (Pseudomonas sp.) and B4M4 (Azospirillum sp.), respectively.

关键词： Beneficial Microorganisms Sorghum bicolor Greenhouse Conditions

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Root colonization by mycorrhizal INIFAP<sup>MR</sup>in barley seed treated with fungicide

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Agricultural Sciences 2013年第12期4卷 738-743页

作者： Oscar A. Grageda-Cabrera Sarahyt S. González-Figueroa Mónica Guadalupe Lozano-Contreras arturo Díaz-franco Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP) Campo Experimental Bajío Celaya México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP) Campo experimental Mocochá Yucatán México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP) Campo experimental Río Bravo Rio Bravo México

The effect of barley seed treated with chlorothalonil fungicide on mycorrhizal root colonization was evaluated. The treatments were: 1) Seed with Mycorrhizal INIFAP?, one, two and three doses, and uninoculated seed;2) Seed with and without fungicide. Monthly seed plantings were performed with different storage time, looking forward to assessing the colonization degree of the roots. As revealed by the results, the seed treated with chlorothalonil did not lower the root colonization by Mycorrhizal INIFAP?;the chlorothalonil treatment had a 32.63% average root colonization, whereas without chlorothalonil, it was 36.46%. When the seed was treated with root colonization by Mycorrhizal INIFAP?, this was lower in the treatment with one dose;no significant difference was revealed by the treatments with either double or triple doses.?The colonization percentage was progressively decreased by seed storage. The root colonization by Mycorrhizal INIFAP?, throughout the inoculated seed storage time, remained constant and unchanged for the first six months, but then decreased in 50% within a 10-month period for both treated and not treated fungicide seed.

关键词： Chlorothalonil Hordeum vulgare Rhizophagus intraradices

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Response of rice cultivation to biofertilizers in Campeche, Mexico

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Agricultural Sciences 2013年第12期4卷 715-720页

作者： Fermín Orona-Castro Mónica Guadalupe Lozano-Contreras Martín Tucuch-Cauich Oscar A. Grageda-Cabrera Juan Medina-Mendez arturo Díaz-franco Esaú Ruiz-Sánchez Jesús Soto-Rocha Campo Experimental Bajío (INIFAP) Celaya México Campo Experimental Río Bravo (INIFAP) Rio Bravo México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP) Campo Experimental Edzna Campeche México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP) Campo Experimental Mococha Yucatán México Instituto Tecnológico de Conkal Conkal México

The activity of various biofertilizers on rice production (Sabanero A95) was evaluated in Palizada, Campeche, Mexico, in the wet season of 2009 (year one) and 2011 (year two). On year one, arbuscular mycorrhiza INIFAP? (Rhizophagus intraradices), Azospirillum brasilense plus arbuscular micorrhiza Rhizophagus sp., and chemical fertilizer (92, -92, -60 kg·ha-1) were evaluated, while on year two marine algae extracts, a consortium of growth promoting bacteria (Pseudomonas spp.) and a control (not fertilized) were evaluated. The results showed that there were no significant differences on grain yield among treatments during the year one. The average grain yield was 2,800 kg·ha-1. As for the year two, the highest grain yield was observed on plots fertilized with chemical fertilizer (3333 kg·ha-1), followed by plots treated with mycorrhiza INIFAP? (3000 kg·ha-1). The economic analysis for rice production in both years showed that the use of arbuscular mycorrhiza decreases the cost of production by 18.5% and 16.3%, which suggests that microbial inoculants might be good substitutes of chemical fertilizers in rice production.

关键词： Rice Wet Season Validation Biofertilizers

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Dengue Climate Variability in Rio de Janeiro City with Cross-Wavelet Transform

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Journal of Environmental Protection 2022年第3期13卷 261-276页

作者： Suellen Araujo franco dos Santos Hugo Abi Karam Augusto José Pereira Filho Jú lio Cesar Barreto da Silva Jose Luis Flores Rojas Julio Migue Angeles Suazo Isela Leonor Vá squez Panduro Cesar arturo Sanchez Peñ a Departamento de Meteorologia Instituto de Geoci&ecirc ncias Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brasil Departamento de Ciê ncias Atmosfé ricas Instituto de Astronomia Geofísica e Ci&ecirc ncias Atmosfé ricas Universidade de S&atilde o Paulo (IAG-USP) S&atilde o Paulo Brasil Programa de Pós-Graduaç ã o em Ciê ncias Ambientais Universidade Estadual do Rio de Janeiro (UERJ) Rio de Janeiro Brasil Instituto Geofísico do Peru (IGP) Lima Per&uacute Facultad de Ingeniería Forestal y Ambiental. Universidad Nacional Autó noma de Tayacaja Daniel Herná ndez Morillo (UNAT) Pampas Per&uacute Universidad Tecnoló gicadel Perú Faculdad de Engenier&iacute a Industrial Lima Per&uacute Centro de Previsã o do Tempo e Estudos Climá ticos Cachoeira Paulista Brasil Programa de Pó s-Graduaç ã o em Computaç ã o Aplicada Instituto Nacional de Pesquisas Espaciais (INPE) S&atilde o José dos Campos S&atilde o Paulo SP Brasil

Dengue is one of the most prominent tropical epidemic diseases present in the Rio de Janeiro city and Southeast part of Brazil, due to the widespread conditions of occurrence of the dengue vector, the mosquito Aedesaegypti, such as high-temperature days interlaced with afternoon or nocturnal rainstorms in summer. This work has the objective of investigating the relationships between variabilities of the El Ni?o-South Oscillation (ENSO) and greater epidemics of dengue in Rio de Janeiro city. To accomplish this goal, the analysis and signal decomposition by cross-wavelet transform (WT) was applied to obtain the cross variability associated with variations of power and phase of both signals by characteristic periods and along with the time series. Data considered in the analysis are (the decimal logarithm of normalized value) of the monthly available notifications of dengue worsening, provided by the public health system of Brazil, and the Southern Oscillation Index (SOI) Ni?o 3.4 data, provided by the National Oceanic and Atmospheric Administration (NOAA), in the period 2000-2017. A maximum cross-wavelet power close to 0.45 was obtained for the representative period of 1 year and also to periods between 3 and 4 years, associated with the positive phase of the SOI index (i.e. , La Ni?a) or with a transition to the positive phase. The evolution of the combined variability of SOI and dengue can be expressed by progressive differences in phase along the time, eventually resulting in yielding phases (i.e., La Niña-Dengue epidemic).

关键词： Dengue Fever Cross-Wavelet Transform Analysis El Niño-Southern Oscillation Index

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Evaluation of the Safety of Three Phenolic Compounds from <i>Dipteryx alata</i>Vogel with Antiophidian Potential

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Chinese Medicine 2015年第1期6卷 1-12页

作者： Edson Hideaki Yoshida Miriéle Cristina Ferraz Natália Tribuiani Renata Vasques da Silva Tavares José Carlos Cogo Márcio Galdino dos Santos Luiz Madaleno franco Cháriston André Dal-Belo Rone A. De Grandis Flávia Aparecida Resende Eliana Aparecida Varanda Pilar Puebla arturo San-Feliciano Francisco Carlos Groppo Yoko Oshima-franco Post-Graduate Program in Pharmaceutical Sciences University of Sorocaba Sorocaba Brazil Serpentarium of the University of Vale do Paraíba S&atildeo José dos Campos Brazil Post-Graduate Program in Environmental Sciences Tocantins Federal University Palmas Brazil Methodist University of Piracicaba Piracicaba Brazil Laboratory of Neurobiology and Toxinology Federal University of Pampa S&atildeo Gabriel Brazil Department of Biological Sciences S&atildeo Paulo State University Araraquara Brazil Department of Pharmaceutical Chemistry Salamanca University Salamanca Spain Department of Physiological Sciences University of Campinas Piracicaba Brazil

Phenolic compounds from Dipteryx alata Vogel were assayed against the in vitro neurotoxic effect induced by Bothrops jararacussu (Bjssu) venom. Mutagenicity was assessed by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100, and TA102, in experiments with and without metabolic activation. Anti-bothropic activity was obtained by using mouse phrenic nerve-diaphragm (PND) preparation and myographic technique. Control experiments with physiological Tyrode solution were used for keeping the PND preparations alive (n = 4). Concentrations of phe-nolic compounds were as follow: protocatechuic and vanillic acids (200 μg/mL, n = 4), vanillin (50 μg/mL, n = 4). These compounds were used alone or pre-incubated with the venom (40 μg/mL), 30 min prior the addition to the organ bath (n = 4). Phenolic compounds significantly inhibited the neuromuscular blockade of Bjssu in the following order of potency: vanillic acid > protocatechuic = vanillin. Vanillic acid added 10 min after the Bjssu venom was also able to avoid the venomblockade evolution. The mutagenicity assay indicated that all phytochemicals were unable to in-crease the number of revertants, demonstrating the absence of mutagenic activity. This study demonstrated both the safety and therapeutical potential of the three phenolic compounds as novel complementary anti-bothropic agents.

关键词： Ames Test Baru Bothrops jararacussu Venom Vanillic Acid Vanillin

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射孔新技术——高性能射孔模型

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国外测井技术 2017年第1期38卷 61-71页

作者： Carlos Baumann Alfredo Fayard Brenden Grove Jeremy Harvey Wenbo Yang Amit Govil Andy Martin Roberto franco Mendez Garcia arturo Ramirez Rodriquez Jock Munro Cesar Velez Terrazas Lang Zhan 斯伦贝谢公司

爆炸聚能射孔是一种在油气井中射穿套管来形成井筒和套管外岩石之间通道的技术。服务公司通过地面试验，将聚能射孔弹射入无应力水泥靶中，以了解其在已知条件下的射孔性能。在认识到模拟程序可能无法正确预测井下聚能射孔弹的性能后，... 详细信息

爆炸聚能射孔是一种在油气井中射穿套管来形成井筒和套管外岩石之间通道的技术。服务公司通过地面试验，将聚能射孔弹射入无应力水泥靶中，以了解其在已知条件下的射孔性能。在认识到模拟程序可能无法正确预测井下聚能射孔弹的性能后，斯伦贝谢的科研人员开发出了可精确计算射孔深度、射孔效率和系统动态响应的软件，并以此来优化在高应力岩石中聚能射孔弹的性能。

关键词：射孔弹射孔枪模型分析井下条件射孔效率射孔密度作业者套管射孔预测模型渗透率

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射孔新技术--高性能射孔模型

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石油工业计算机应用 2017年第3期25卷 16-25页

作者： carlos baumann alfredo fayard brenden grove jeremy harvey wenbo yang amit govil andy martin roberto franco mendez garcia arturo ramirez rodriquez jock munro cesar velez terrazas lang zhan 美国得克萨斯州Rosharon 挪威Tananger 英国剑桥墨西哥国家石油公司(pemex) 墨西哥韦拉克鲁斯Agua Dulce 苏格兰阿伯丁墨西哥塔巴斯科州Villahermosa 壳牌石油公司得克萨斯州休斯顿

爆炸聚能射孔是一种在油气井中射穿套管来形成井筒和套管外岩石之间通道的技术。服务公司通过地面试验，将聚能射孔弹射入无应力水泥靶中，以了解其在已知条件下的射孔性能在认识到模拟程序可能无法正确预测井下聚能射孔弹的性能后，斯... 详细信息

爆炸聚能射孔是一种在油气井中射穿套管来形成井筒和套管外岩石之间通道的技术。服务公司通过地面试验，将聚能射孔弹射入无应力水泥靶中，以了解其在已知条件下的射孔性能在认识到模拟程序可能无法正确预测井下聚能射孔弹的性能后，斯伦贝谢的科研人员开发出了可精确计算射孔深度、射孔效率和系统动态响应的软件，并以此来优化在高应力岩石中聚能射孔弹的性能。

关键词：聚能射孔弹孔性能技术孔模型地面试验服务公司模拟程序已知条件

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