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Inf.uence of.previous plane of.nutrition on molecular mechanisms regulating the expression of.urea and water metabolism related genes in the rumen and kidney of.f.nishing crossbred Angus steers

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Animal Nutrition 2024年第2期17卷 232-243页

作者： Aghata E.moreira da silva arturo macias f.anco Bradley s.f.rguson mozart A.f.nseca College of.Agriculture Biotechnology&Natural Resources University of Nevada Department of.Animal and Range sciences Clayton Livestock Research Center New Mexico State University

This study aimed to unde.stand how molecular mechanisms controlling water and urea metabolism at the f.nishing phase can be af.ected by previous plane of.nutrition of.crossbred Angus beef.steers. Twentyf.ur(n = 24) animals were randomly distributed into either a moderate(mP) or high plane of.nutrition during the background phase f.r 85 d. Animals were then blocked by their previous plane and were moved onto a 105-d f.nishing phase in a 2 × 2 f.ctorial arrangement. The f.rage-f.nished group received only high-quality alf.lf. hay, whereas the grain-f.d group received a high grain diet(80% whole corn and20% alf.lf. hay). By the end of.the f.nishing phase, animals were harvested and tissue samples f.om the rumen and kidney were collected. Changes in gene expression of.aquaporins(AQP)-2,-3,-4,-7, ATP1A1,ATP1B1, sGK1, CLIC1(kidney and rumen), UT-A1(kidney only) and UT-B(rumen only), were assayed via real-time q PCR; 18s r RNA was used as an endogenous control. One-way ANOVA f.llowed by Tukey's post hoc analysis was conducted. When animals were f.om mP, f.rage-f.nishing increased the relative abundance of.AQP3(P ≤ 0.05), AQP7(P ≤ 0.05), ATP1B1(P ≤ 0.05), and sGK1(P ≤ 0.05) in the kidney when compared to grain-f.d animals. In the rumen, f.r the mP group, AQP7 was dif.erentially expressed in both treatments at the f.nishing phase(P ≤ 0.01), with f.rage-f.nished steers having the highest expression of.AQP7. f.r the mP group, UT-B had a tende.cy of.presenting a higher expression on grainf.d animals(P = 0.075). Overall, these results suggest that previous plane can impact expression of.genes associated with water and urea metabolism during the f.nishing phase, namely AQP3, AQP7, ATP1B1, and sGK1 in the kidney, and AQP7 and UT-B in the rumen. The greatest impact observed on gene expression changes of.investigated genes at the f.nishing phase was ref.ective of.animals backgrounde. on the moderate previous plane.

关键词： Gene expression Aquaporin sodium channel Urea transporter

mgO-attached graphene nanosheet(mgO@GNs)reinf.rced magnesium matrix nanocomposite with superior mechanical,corrosion and biological perf.rmance

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International Journal of.minerals,metallurgy and materials 2024年第9期31卷 2062-2076页

作者： s.Abazari A.shamsipur H.R.Bakhsheshi-Rad m.s.soheilirad f.Khorashadizade s.s.mirhosseini Department of.materials and metallurgical Engineering Amirkabir University of TechnologyTehran ***Iran Iranian National standards Organization of.Hormozgan HormozganIran Advanced materials Research Center Department of Materials EngineeringNajafabad BranchIslamic Azad UniversityNajafabadIran Department of.materials and metallurgical Engineering Iran University of Science and TechnologyTehranIran

magnesium(mg)alloys are gaining great consideration as body implant materials due to their high biodegradability and ***,they suf.er f.om low corrosion resistance and antibacterial *** this research,semi-powder metallurgy f.llowed by hot extrusion was utilized to produce the magnesium oxide@graphene nanosheets/magnesium(mgO@GNs/mg)composite to improve mechanical,corrosion and cytocompatibility *** have revealed that the incorporation of.mgO@GNs nanohybrids into mg-based composite enhanced microhardness and compressive *** vitro,osteoblast cell culture tests show that using mgO@GNs nanohybrid f.llers enhances osteoblast adhesion and apatite *** presence of.mgO@GNs nanoparticles in the composites decreased the opening def.cts,micro-cracks and micro-pores of.the composites thus preventing the penetration of.the corrosive solution into the *** demonstrated that the mgO@GNs/mg composite possesses excellent antibacterial properties because of.the combination of.the release of.mgO and physical damage to bacterium membranes caused by the sharp edges of.graphene nanosheets that can ef.ectively damage the cell wall thereby f.cilitating penetration into the bacterial lipid ***,the mgO@GNs/mg composite with high mechanical strength,antibacterial activity and corrosion resistance is considered to be a promising material f.r load-bearing implant applications.

关键词： metal matrix composites mgO@GNs nanohybrid strengthening mechanisms antibacterial activity biocompatibility

The asymptotic behavior f.r a binary alloy in energy and material science:The unif.ed method and its applications

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Journal of.Ocean Engineering and science 2024年第4期9卷 373-378页

作者： m.Adel K.Aldwoah f.Alahmadi m.s.Osman Department of.mathematics Faculty of ScienceIslamic University of MadinahMedinaSaudi Arabia Department of.mathematics Faculty of ScienceCairo UniversityGiza 12613Egypt

The Cahn-Hilliard system was proposed to the f.rst time by Chan and Hilliard in *** model(or system of.equations)has intrinsic participation energy and materials sciences and depicts signif.cant characteristics of.two phase systems relating to the procedures of.phase separation when the temperature is *** instance,it can be noticed when a binary alloy(“Aluminum+Zinc”or“Iron+Chromium”)is cooled down *** this case,partially or totally nucleation(nucleation means the appearance of.nuclides in the material)is observed:the homogeneous material in the initial state gradually turns into inhomogeneous,giving rise to a very accurate dispersive ***,when the time scale is slower the microstructure becomes *** this work,to the f.rst time,the unif.ed method is presented to investigate some physical interpretations f.r the solutions of.the Cahn-Hilliard system when its coef.icients varying with time,and to show how phase separation of.one or two components and their concentrations occurs dynamically in the ***,2D and 3D plots are introduced to add more comprehensive study which help to unde.stand the physical phenomena of.this *** technique applied in this analysis is powerf.l and ef.icient,as evidenced by the computational work and *** technique can also solve a large number of.higher-order evolution equations.

关键词： Cahn-Hilliard system Traveling wave solutions The unif.ed method Variable coef.icients

Control and vibration analyses of.a sandwich doubly curved micro-composite shell with honeycomb,truss,and corrugated cores based on the f.urth-order shear def.rmation theory

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Applied mathematics and mechanics(English Edition) 2024年第10期45卷 1773-1790页

作者： f.sHIRDELAN m.mOHAmmADImEHR f.BARGOZINI Department of.solid mechanics Faculty of Mechanical EngineeringUniversity of KashanKashan 87317-53153Iran

Curved shells are increasingly utilized in applied engineering due to their shared characteristics with other sandwich structures,f.exibility,and attractive ***,the inability of.controlling and regulating vibrations and destroying them af.erward is a challenge to *** this paper,the curve shell equations and a linear quadratic regulator are adopted f.r the state f.edback design to manage the structure vibrations in state space f.rms.A f.ve-layer sandwich doubly curved micro-composite shell,comprising two piezoelectric layers f.r the sensor and actuator,is modeled by the f.urth-order shear def.rmation *** core(honeycomb,truss,and corrugated)is analyzed f.r the bearing of.transverse shear *** results show that the honeycomb core has a greater ef.ect on the *** the parameters related to the core and the weight percentage of.graphene increase,the f.equency *** unif.rm distribution of.graphene platelets results in the lowest natural f.equency while the natural f.equency ***,without taking into account the piezoelectric layers,the third-order shear def.rmation theory(TsDT)and f.urth-order shear def.rmation theory(f.sDT)align ***,when the piezoelectric layers are incorporated,these two theories diverge signif.cantly,with the f.equencies in the f.sDT being lower than those in the TsDT.

关键词： vibration and control doubly curved shell micro-composite honeycomb truss corrugate core

The viscous strip approach to simplif. the calculation of.the surf.ce acoustic wave generated streaming

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Applied mathematics and mechanics(English Edition) 2024年第4期45卷 711-724页

作者： f.JAZINI DORCHEH m.GHAssEmI f.el Cells and Nano systems(f.Ns)Laboratory Department of Mechanical EngineeringK.N.Toosi University of TechnologyTehran 19697Iran

In recent decades,the importance of.surf.ce acoustic waves,as a biocompatible tool to integrate with microf.uidics,has been proven in various medical and biological *** numerical modeling of.acoustic streaming caused by surf.ce acoustic waves in microchannels requires the ef.ect of.viscosity to be considered in the equations which complicates the *** this paper,it is shown that the major contribution of.viscosity and the horizontal component of.actuation is concentrated in a narrow region alongside the actuation *** the inviscid equations are considerably easier to solve,a division into the viscous and inviscid domains would alleviate the computational load *** particles'traces calculated by this approximation are excellently alongside their counterparts f.om the completely viscous *** is also shown that the optimum thickness f.r the viscous strip is about 9-f.ld the acoustic boundary layer thickness f.r various f.ow patterns and amplitudes of.actuation.

关键词： surf.ce acoustic wave microf.uidics numerical simulation particle tracing acoustic streaming

RPLP0/TBP are the most stable ref.rence genes f.r human dental pulp stem cells unde. osteogenic dif.erentiation

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World Journal of.stem Cells 2024年第6期16卷 656-669页

作者： Daniel B f.rreira Leticia m Gasparoni Cristiane f.Bronzeri Katiucia B s Paiva Laboratory of.Extracellular matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of.the ref.rence gene(RG)stability during experimental analyses is essential f.r correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular f.nctions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18s rRNA,andβ-actin]without paying attention to whether they are suitable f.r such experimental conditions or the reason f.r choosing such ***,such studies use only one gene when minimum Inf.rmation f.r Publication of.Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of.these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of.gene expression drives the best experimental or therapeutical *** cultivated DPsCs unde. two conditions:Undif.erentiated and osteogenic dif.f.rentiation,both f.r 35 *** evaluated the gene expression of.10 candidates f.r RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAs1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation f.ctor 1 alpha(Ef.a),succinate dehydrogenase complex,subunit A,f.avoprotein(sDHA),and beta-2-micro-globulin(B2m)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the f.ur main algorithms,ΔCt method,geNorm,Normf.nde.,and BestKeeper and ranked by the Ref.inde. *** subdivided the samples into eight *** All of.the data sets f.om clonogenic and osteogenic samples were analysed using the Ref.inde. *** f.nal ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2m as the two least stable *** theΔCt method or Normf.nde. analysis showed TBP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/Ef.αin the f.rst *** algorithms’two least stab

关键词： Dental pulp stem cells Ref.rence gene Housekeeping gene Endogenous gene Osteogenic dif.erentiation Ref.inde.

Engineering of.ef.icient visible light photocatalysts:Ti_(1-x+y)Cu_(x)La_(y)O_(2)(x=0.03;y=0,0.005,0.01) compositions

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Journal of.Rare Earths 2024年第3期42卷 497-505,I0003页

作者： s.m.Yakout A.m.Youssef Inorganic Chemistry Department National Research Centre(NRC)El Buhouth StDokkiCairo12622Egypt

In dye-photocatalysis, widening the wavelengths absorption range of.catalysts as well as aborting the recombination of.the photoexcited charge carriers(electrons-holes pairs) are of.paramount *** the current study, we report the photocatalytic characteristics of.f.ur compositions composed of.TiO_(2),Ti_(0.97)Cu_(0.03)O_(2), Ti_(0.965)Cu_(0.03)La_(0.005)O_(2)and Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)powders f.r two harmf.l dyes(Congo red and methylene blue). The X-ray dif.raction technique verif.ed the conf.guration of.mixed anatase and rutile phases in pure TiO_(2)and Ti_(0.97)Cu_(0.03)O_(2)powders while Ti_(0.965)Cu_(0.03)La_(0.005)O_(2)and Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)powders possess anatase phase only. The changes of.the band gap structure and dimensions of.the unit cell volume of.TiO_(2)conf.rm the substitution of.Ti^(4+)sites by Cu^(2+)and La^(3+)ions. Remarkable widening of.wavelengths absorption with f.rmation of.long absorption tails were f.und in TiO_(2)(3.13 e V) through insertion of.La^(3+)plus Cu^(2+)ions. Ti_(0.965)Cu_(0.03)La_(0.005)O_(2)and Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)samples exhibit a visible light band gap of.2.3 and 2.7 e V, respectively. Based on sEm images, incorporation of.Cu^(2+)and La^(3+)ions inf.uences the surf.ce morphology of.TiO_(2). The resulting Ti_(0.97)Cu_(0.03)O_(2), Ti_(0.965)Cu_(0.03)La_(0.005)O_(2)and Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)powders present enhanced photocatalytic activity towards Congo red dye degradation compared to pure one unde. natural solar spectrum. The best Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)catalyst exhibits superior photocatalytic activity of.96% f.r 10 ppm CR dye in 20 min as well as above average photocatalytic ef.iciency of.63% f.r 30 ppm CR dye in 120 min. As well, it showed acceptable photodegradation activity of.52% f.r 30 ppm mB solution in 140 min of.sunlight irradiation. The heightened photodegradation ef.iciency of.Ti_(0.96)Cu_(0.03)La_(0.01)O_(2)catalyst can be clarif.ed in terms of.higher ch

关键词： semiconductor TiO_(2) Rare earths Congo red methylene blue solar spectrum Photodegradation

Ef.ect of.solidif.cation cooling rate on microstructure and tribology characteristics of.Zn-4si alloy

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International Journal of.minerals,metallurgy and materials 2024年第2期31卷 362-373页

作者： f.Akbari m.Golkaram s.Beyrami G.shirazi K.mantashloo R.Taghiabadi m.saghaf. Yazdi I.Ansarian Department of.metallurgy and material science Imam Khomeini International University(IKIU)Qazvin ***Iran

The main objective of.this work was to modif. the microstructure and enhance the tribological properties of.a new Zn-4si al-loy through a high solidif.cation cooling rate(sCR).According to the results,by increasing the sCR f.om 2.0 to 59.5℃/s the average size of.primary si particles and that of.the grains reduced f.om 76.1 and 3780μm to less than about 14.6 and 460μm,***-ing the sCR also enhanced the microstructural homogeneity,decreased the porosity content(by 50%),and increased the matrix hardness(by 36%).These microstructural changes enhanced the tribological *** instance,unde. the applied pressure of.0.5 mPa,an in-crease in the sCR f.om 2.0 to 59.5℃/s decreased the wear rate and the average f.iction coef.icient of.the alloy by 57%and 23%,*** wear mechanism was also changed f.om the severe delamination,adhesion,and abrasion in the slowly-cooled alloy to the mild tribolayer delamination/abrasion in the high-cooling-rate-solidif.ed sample.

关键词： zinc-silicon alloy primary silicon solidif.cation cooling rate tribology sliding wear

Analyzing study f.r the 3D potential Yu-Toda-sasa-f.kuyama equation in the two-layer liquid medium

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Journal of.Ocean Engineering and science 2024年第5期9卷 454-460页

作者： Hajar f.Ismael Ìsraf.l Okumus Tolga Aktürk Hasan Bulut m.s.Osman Department of.mathematics Faculty of ScienceUniversity of ZakhoZakhoIraq Department of.mathematics Faculty of ScienceFirat UniversityElazigTurkey Department of.mathematics Faculty of ScienceErzincan Binali Yildirim UniversityErzincanTurkey Department of.mathematics and science Education Faculty of EducationOrdu UniversityTurkey Department of.mathematics Faculty of Applied ScienceUmm Al-Qura UniversityMakkah 21955Saudi Arabia Department of.mathematics Faculty of ScienceCairo UniversityGiza 12613Egypt

In this work,we use the(m+1/G')-expansion method and the Adomian decomposition method to study the 3D potential Yu-Toda-sasa-f.kuyama(3D-pYTsf.equation which has a good application in the twolayer liquid *** the f.rst time,the(m+1/G')-expansion and the Adomian decomposition methods are used to establish novel exact wave solutions and to study some numerical solutions f.r the 3D-pYTsf.equation,*** using the analytical method,kink-type wave,singular solution and some complex solutions to the suggested equation are successf.lly *** obtained wave solutions are represented with some f.gures in 3D and contour plots.

关键词： (m+1/G')-expansion method Complex solutions Adomian decomposition method