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Doxorubicin Induces Apoptosis through down Regulation of miR-21 Expression and Increases miR-21 Target Gene Expression in MCF-7 Breast Cancer Cells

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International Journal of Clinical Medicine 2017年第6期8卷 386-394页

作者： Roghayeh Tofigh Saeedeh Akhavan Nastaran Tarban Amin Ebrahimi Sadrabadi arsalan Jalili Kaykhosro Moridi Sara Tutunchi Department of Animal Biology Tabriz University Tabriz Iran Department of Biology School of Basic Sciences Science and Research Branch Islamic Azad University (IAU) Tehran Iran Department of Biology Kish International Campus University of Tehran Kish Iran Department of Cell Engineering Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Department of Stem Cells and Developmental Biology at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Department of Biology Faculty of Advanced Sciences and Technology Pharmaceutical Sciences Branch Islamic Azad University (IAUPS) Tehran Iran Department of Medical Genetics Shahid Sadoughi University of Medical Sciences Yazd Iran

miRNAs play an important regulatory role in variety of cellular functions and several diseases, including cancer. MicroRNA-21 (miR-21) is overexpressed in almost all types of human cancers. Studies revealed that the knockdown of miR-21 results in reduced tumor cell growth, cell cycle arrest and cell apoptosis. In this study, we evaluated the effect of doxorubicin on miR-21 expression in mcf-7 breast cancer cells. miRNA was extracted from mcf-7 cells treated with doxorubicin and untreated cells using miRNeasy Kit (Qiagen) according to the manufacturer’s instructions. cDNA synthesis was performed using miScript II RT Kit (Qiagen) and Real Time-PCR was performed using Real Q Plus 2x Master Mix Green-(Ampliqon, Denmark). The relative expression of miR-16 and miR-21 was calculated using comparative Ct method. All tests were run in triplicate to minimize the experimental errors. Samples with a Ct > 37 were excluded from the analysis. Statistically, a significant decrease in cell proliferation of mcf-7 cells was found in doxorubicin group compared with control groups 24 hours after transfection, dose dependently (p value< 0.001). After 24 hours, Doxorubicin (100 μm) significantly decreased miR-21 expression in mcf-7 cells (p = 0.0001). Also, the expression of caspase 9 significantly increased after Doxorubicin (100 μm) treatment (p = 0.0003). Together, these findings indicate that miR-21 plays a key role in regulating cell apoptosis in mcf-7 cells and may serve as a target for effective therapies.

关键词： miR-21 mcf-7 Cells Caspase 9 Cancer

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基于太阳能供电的水泵灌溉系统设计

基于太阳能供电的水泵灌溉系统设计

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作者：阿萨兰（arsalan RAZA）华北电力大学

学位级别：硕士

At present there are lots of energy sources which are fulfilling our energy demands. And in all of them renewable energy is the most reliable one as compare to other sources. The sun provides Earth with limitless sources of energy sustaining all humanity demands. Before it was unable for a common person to avail the opportunity of solar energy because of the initial cost. But a lot of work has done on this except on agriculture sector in Pakistan. Since solar power is not being used in agriculture sector as compare to other developed countries so I think this project can bring a lot change and save a lot of energy just for agriculture system. Irrigation is a well-established procedure on many farms in all the Europe countries and is practiced on various levels around the world. It allows diversification of crops, while increasing crop yields. However, typical irrigation systems consume a great amount of conventional energy through the use of electric motors and generators powered by fuel This Project is about running a water pump by solar energy. It will explain the use of non-conventional energy source for our daily, house hold use. Solar energy provides a solution to energy crisis problem in our country. This concerned project deals with this problem in an efficient way by providing energy continuously and independently. This energy is then utilized by water pumps for irrigation purposes, livestock watering and village water supply systems. We used solar panel of 14" X 9" dimension. With an active solar tracker driven by a DC gear motor. For charge storage we used sealed lead acid battery and charge controller to control its charging. 12 VDC pump is used for water pumping purpose. Solar energy is one of the best energy we had in recent time of period and it not only provides a lot facility but also is much economical. All the experiments are done by organized team which led us to get better results.

关键词：水泵灌溉系统节能设计太阳能供电

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Contrast-Induced Nephropathy in Patients Undergoing Elective Coronary Angiography： Incidence and Risk Factors

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Journal of Health Science 2016年第3期4卷 111-117页

作者： arsalan Salari Fardin Mirbolouk Bijan Shad Mahboobeh Gholipour Tolou Hasandokht Amin Karimi Azam Nourisaeed Samaneh Habibnejad Guilan Interventional Cardiovascular Research Center Guilan University of Medical Sciences Rasht Iran

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柏林艺术论坛重装出击

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东方艺术 2009年第21期 24-25页

作者： arsalan Mohammad 石八平

＂坦率地说,我们想知道我们是否应该这么做＂他说,＂我们知道市场在复苏,我认为这需要花费两到三年时间。人们忘了我们曾在2001年遭受经济衰退,但那只是很小的经济危机,这次的情形要糟糕十倍。人们都说衰退已经结束,但我觉得他们根本不... 详细信息

＂坦率地说,我们想知道我们是否应该这么做＂他说,＂我们知道市场在复苏,我认为这需要花费两到三年时间。人们忘了我们曾在2001年遭受经济衰退,但那只是很小的经济危机,这次的情形要糟糕十倍。人们都说衰退已经结束,但我觉得他们根本不知道自己在说什么。因此,我认为有些展商是绝对平稳的。而我们正在做事情的比较冒险,但是成功了。＂

关键词：出击论坛艺术柏林经济衰退经济危机

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丹参对外周血中性粒细胞和单核细胞的调整作用

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广西医科大学学报 1997年第3期14卷 9-11页

作者：王潮临 arsalan Kharazmi Viggo Faber 广西医科大学病理生理学教研室丹麦哥本哈根大学医院临床微生物部

研究丹参对外周血中性粒细胞和单核细胞的化学趋化和超氧化物产物的影响。结果表明：丹参能明显增加两种细胞的超氧化物的产生；并对两种细胞的化学趋化有明显的抑制作用，丹参能调整中性粒细胞的氧化过程（呼吸爆发），参与化学趋化作... 详细信息

研究丹参对外周血中性粒细胞和单核细胞的化学趋化和超氧化物产物的影响。结果表明：丹参能明显增加两种细胞的超氧化物的产生；并对两种细胞的化学趋化有明显的抑制作用，丹参能调整中性粒细胞的氧化过程（呼吸爆发），参与化学趋化作用、炎症过程和增强中性粒细胞的杀菌能力。

关键词：丹参化学趋化超氧化物中性粒细胞单核细胞

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Assessment of Biochemical and Antioxidative Status in Patients Suffering from Dengue Fever

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2015年第3期35卷 411-418页

作者： Mahmood Rasool Arif Malik Khalid Mahmud Khan Muhammad Saeed Qureshi Beenish Shabbir Sara Zahid Muhammad Asif Abdul Manan Sana Rashid Saima Rubab Khan Hafiz Muhammad arsalan Rabail Alam Mahwish Arooj Mahmood Husain Qazi Adeel Gulzar Ahmed Chaudhary Adel Mohammed Abuzenadah Mohammed Hussain Al-Qahtani Sajjad Karim Center of Excellence in Genomic Medicine Research Faculty of Applied Medical SciencesKing Abdulaziz University Institute of Molecular Biology and Biotechnology the University of Lahore Fatima Jinnah Medical College Sir Gangaram Hospital Lahore Institute of Molecular Biology and Biotechnology the University of Lahore Department of Biotechnology and Informatics BUITEMS Quetta 87300 Pakistan Center for Research in Molecular Medicine the University of Lahore KACST Technology Innovation Center in Personalized Medicine King Abdulaziz University Center of Excellence in Genomic Medicine Research Faculty of Applied Medical Sciences King Abdulaziz University

Summary：A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever（DF） by evaluating biochemical and oxidative status of patients. ELISA Ig M antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever（DHF） and dengue shock syndrome（DSS） showed significant difference. Significant increases in serum alanine amino transferase（ALT）（P=0.000）, aspartate amino transferase（AST）（P=0.000）, alkaline phosphatase（ALP）（P=0.000）, malondialdehyde（MDA） along with significant decreases in total protein（TP）（P=0.000）, reduced glutathione（GSH）（P=0.000）, superoxide dismutase（SOD）, catalase（CAT）（P=0.000）, and sialic acid contents（P=0.016） were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants（r=0.656, P=0.016）. In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.

关键词： dengue fever alanine amino transferase aspartate amino transferase malondialdehyde superoxide dismutase catalase

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Frequency of Various Risk Factors in HCV Positive Patients in Initial Diagnostic Phase

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Occupational Diseases and Environmental Medicine 2018年第2期6卷 23-36页

作者： Anwar Ali Jamali Ghulam Mustafa Jamali Ameer Ali Jamali Bhojo Mal Tanwani Niaz Hussain Jamali arsalan Ahmer Rajput Moti Ram Bhatia Department of Medicine Peoples University of Medical and Health Sciences Nawabshah Pakistan Department of Pediatrics Peoples University of Medical and Health Sciences Nawabshah Pakistan Department of Physiology Peoples University of Medical and Health Sciences Nawabshah Pakistan Department of Pharmacy Peoples University of Medical and Health Sciences Nawabshah Pakistan Department of Psychiatry Peoples University of Medical and Health Sciences Nawabshah Pakistan

Viral hepatitis C is a type of illness, which is transmitted to patients by different methods in world. Here we will identify the different common risk factors for transmission of viral hepatitis C in our setup. Aim of study was to determine frequency of various risk factors in HCV positive patients. This study was cross sectional and conducted Department of Medicine PMC Hospital at Nawabshah. Duration of this study was one year from April 2016 to March 2017. After taking informed written consent, 243 diseased persons with positive anti Hepatitis C Antibodies were incorporated in this research. Frequency of variables i.e. Hepatitis C virus risk factors and different other demographic results was collected on preformed proforma. In total of 243 subjects there were 165 (67.9%) male and 78 (32.1%) females respectively. Most of the subjects have more than one risk factor. 19 (7.8%) had history of blood and blood products transfusion. IV drug abuse was detected in 08 (3.3%). Homosexuality and heterosexuality were observed in 14 (5.8%) and 12 (4.9%) subjects respectively. History of dental procedure was seen in 31 (12.8%). 228 (93.8%) had history of needle pricking in different ways. History of different surgical procedures was observed in 33 (13.8%). Calculated Mean and Standard Deviation for age was 39.2 ± 10.3 years. It was concluded that proper implementation of precautionary measures should be carried out for every human being to reduce burden of HCV illness in far near future.

关键词： Viral Hepatitis C Anti HCV Antibodies Risk Factors

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美国重启制裁伊朗何去何从

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商业周刊（中文版） 2018年第15期 38-40页

作者： Ladane Nasseri Golnar MoCevalli arsalan Shahla 许诺(译)

伊核协议破裂给伊朗未来增添了不确定性，目前这种严峻形势还有进一步恶化的可能随着美国8月7日恢复对伊朗实施制裁，处于困境的伊朗总统哈桑·鲁哈尼（Hassan Rouhani）收到了从这个伊斯兰共和国的几大阵营传出的严厉指令。伊朗最高领... 详细信息

伊核协议破裂给伊朗未来增添了不确定性，目前这种严峻形势还有进一步恶化的可能随着美国8月7日恢复对伊朗实施制裁，处于困境的伊朗总统哈桑·鲁哈尼（Hassan Rouhani）收到了从这个伊斯兰共和国的几大阵营传出的严厉指令。伊朗最高领袖阿里·哈梅内伊（Ali Khamenei）敦促他治理腐败。

关键词：伊朗制裁美国不确定性哈梅内伊治理腐败共和国伊斯兰

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一种新的黄芩类黄酮3',5,6',7-四羟基-2',8-二甲氧基黄酮的体外免疫药理作用

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广西医科大学学报 2000年第3期17卷 353-355页

作者：贺海平秦箐陈明 arsalan Kharazmi Soren Brogger Christensen 广西肿瘤防治研究所南宁530021 广西医科大学南宁530021 丹麦国家医院临床微生物学研究室哥本哈根22200

目的 :对新发现的一种黄芩类黄酮 ,3',5 ,6 ',7-四羟基 - 2 ',8-二甲氧基黄酮 ( TDF)的免疫药理作用进行研究。方法 :检测 TDF对人多形核细胞 ( PMN)、单个核细胞 ( MNC)化学发光反应、化学趋向性和 PHA诱导淋巴细胞增殖的影响。结果 :... 详细信息

目的 :对新发现的一种黄芩类黄酮 ,3',5 ,6 ',7-四羟基 - 2 ',8-二甲氧基黄酮 ( TDF)的免疫药理作用进行研究。方法 :检测 TDF对人多形核细胞 ( PMN)、单个核细胞 ( MNC)化学发光反应、化学趋向性和 PHA诱导淋巴细胞增殖的影响。结果 :TDF本身不能激活 PMN和 MNC的氧呼吸爆发 ,TDF对 PMN和 MNC无毒性作用 ;当 TDF与 PMN或 MNC预先孵育后 ,明显的抑制 f ML P或 OZ激活 PMN以及 f ML P激活 MNC的氧呼吸爆发 ,抑制的强度与孵育的剂量成正比关系 ;对PMN的抑制能力大于 MNC。 TDF本身对 PMN和 MNC没有明显的化学趋化作用 ,但经与 TDF预先与细胞孵育后的 PMN或 MNC对 ZAS的化学趋向性明显增强 ,对 f ML P的化学趋向性作用不明显 ;TDF浓度在 ( 1.2 5× 10 - 4～ 5× 10 - 4 ) mol/ L 时 ,显著地抑制 PHA诱导的淋巴细胞增殖 ,抑制率达 31%～ 10 0 %。结论

关键词：黄芩类黄酮 TDF 免疫药理化学发光

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特朗普留下的伊朗“粒摊子”不能只靠拜登修复

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商业周刊（中文版） 2020年第22期 32-33页

作者： arsalan Shahla Francois De Beaupuy Tara Patel Paul Wallace 丁虹(译) 不详

伊朗总统哈桑,鲁哈尼(Hassan Rouhani)表示,拜登修复伊朗和美国之间的关系“非常容易”。他在周三(11月25日)的电视讲话中表示:“这个棘手问题是可以解决的,只要有意愿和决心。”然而,只消看看伊朗千疮百孔的经济便知,特朗普带来的很多... 详细信息

伊朗总统哈桑,鲁哈尼(Hassan Rouhani)表示,拜登修复伊朗和美国之间的关系“非常容易”。他在周三(11月25日)的电视讲话中表示:“这个棘手问题是可以解决的,只要有意愿和决心。”然而,只消看看伊朗千疮百孔的经济便知,特朗普带来的很多破坏并不会一夜消失。

关键词：特朗普伊朗总统电视讲话棘手问题鲁哈尼

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