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Phytogenic Synthesis of Metal/Metal Oxide Nanoparticles for Degradation of Dyes

Journal of Renewable Materials 2022年第7期10卷 1911-1930页

作者： arpita roy H.CAnanda Murthy Hiwa M.Ahmed Mohammad Nazmul Islam Ram Prasad Department of Biotechnology School of Engineering&TechnologySharda UniversityGreater Noida201310India Department of Applied Chemistry School of Applied Natural ScienceAdama Science and Technology UniversityAdama1888Ethiopia Sulaimani Polytechnic University Slemani46001Iraq Department of Horticulture University of RaparinRanya46012Iraq Department of Pharmacy International Islamic University ChittagongChittagong4318Bangladesh Department of Botany Mahatma Gandhi Central UniversityMotihari845401India

Now-a-days nanotechnology is one of the booming fields for the researchers.With the increase in industrialization mainly textile,paper,medicine,plastic industry,there is an increase in concentration of organic dyes as pollutant.Release of harmful dyes in water bodies has become a serious issue,as most of the dyes are carcinogenic and mutagenic in nature and causes various diseases.Therefore,there is a requirement to find out new approaches for efficient treatment of effluent containing dyes.Nanoparticles are one of the potential solutions to this problem.They can be synthesized from different methods,however synthesis of nanoparticles from different plant parts(leaf,root or stem extract)is economical as well as ecofriendly.Phytogenic nanoparticles have various environmental applications and one of them is remediation of dyes.The aim of this review is to provide an overview of last five years studies about catalytic and photocatalytic degradation of various harmful dyes by plant synthesized nanoparticles,mechanism of degradation and advantages and disadvantages of phytogenic synthesis.

关键词： Nanoparticles green synthesis plants dyes remediation,metal oxide

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Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 960-966页

作者： Shreya Mandal arpita Patra Animesh Samanta Suchismita roy arpita Mandal Tapasi Das Mahapatra Shrabani Pradhan Koushik Das Dilip Kumar Nandi Department of Nutrition Microbiology and Human PhysiologyRaja N L Khan Women's College

Objective:To investigate phytochemical screening,antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components,antioxidant activity and total flavonoid compound of Terminalia arjuna.Methods:For phytochemical screening,some common and available standard tests were done.Antimicrobial bioassay was done through agar well diffusion method.Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography.Total antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl(DPPH)in colorimetric method.Aluminum chloride colorimetric method was used for total flavonoid determination.Results:Phytochemical screening showed the active compounds presence in high concentration,such as phytosterol,lactones,flavonoids,phenolic compounds and tannins and glycosides.The anlimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria.This methanolic extract showed a promising antioxidant activity,as absorption of DPPH redicles decreased in DPPH free radical scavenging assay.Flavonoids components having antioxidant property present in the methanol extract at a level of199.00 mg quercetin equivalent/g of dried methanol extract in colorimetric method.Conclusions:The Terminalia arjuna bark extract revealed the presence of bio-active constituents which are known to exhibit medicinal as well as physiological activities.

关键词： Terminalia arjuna Phytocompound TLC Antioxidant Total flavonoid Antimicrobial activity DPPH

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Insight to the Mode of Action of Allium sativumLeaf Agglutinin (ASAL) Expressing in T3Rice Lines on Brown Planthopper

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American Journal of Plant Sciences 2013年第2期4卷 400-407页

作者： arpita Bala Amit roy Niranjana Behura Daniel Hess Sampa Das Division of Plant Biology Bose Institute Kolkata India The Protein Analysis Facility Friedrich Miescher Institute for Biomedical Research Basel The Switzerland

Brown planthopper, the sap sucking hemipteran pest, is one of the major contributors to the yield loss of rice through the world. To combat the situation researchers are interested identifying genes from plant origin having potentiality to develop hemipteran pest resistance. Interestingly, it was observed that rice plants expressing ASAL, a monocot mannose binding lectin, showed significant resistance to brown planthopper and green leafhopper. Additionally, antibiotic resistant marker gene free ASAL expressing rice lines were developed to overcome the biosafety issues. However, the basis behind the resistance against planthoppers is still not clearly understood. Ligand blot assay was performed with total BBMV protein from BPH and a ~56 kDa receptor protein was detected. LC MS/MS analysis revealed that the receptor protein is NADH quinone oxidoreductase (NQO), a key player in electron transport chain, insect defense response and male/female gametogenesis. Presumably interaction of ASAL with NQO may lead to toxicity and loss of fecundity among BPH feeding on ASAL expressing transgenic rice plants. These findings provide a stable scientific basis for considering these transgenic ASAL expressing rice plants as significant product for combating BPH attack associated yield loss of rice.

关键词： Allium sativum leaf agglutinin (ASAL) Hemipteran Pest Management Brown Planthopper NADH Qui-none Oxidoreductase (NQO) Fecundity Transgenic Rice Brush Boarder Membrane Vesicle (BBMV) Protein

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An Approach to the Identification and Characterisation of a Psychrotrophic Lipase Producing Pseudomonassp ADT3 from Arctic Region

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Advances in Bioscience and Biotechnology 2014年第4期5卷 322-332页

作者： arpita Dey Amarnath Chattopadhyay Pradipta Saha Subhrakanti Mukhopadhyay Tushar Kanti Maiti Sabyasachi Chatterjee Pranab roy Department of Biotechnology The University of Burdwan Burdwan India Department of Botany The University of Burdwan Burdwan India Department of Microbiology The University of Burd-wan Burdwan India

The aim of this research work was to explore psychrotrophic microbes from soil sample of NyAlesund, Svalbard, arctic region and to investigate their potential use as an effective tool for industrial application. A novel psychrotrophic bacterial strain showed good growth on minimal medium containing lipid as the only carbon source. Microbiological characterisation of the isolate showed that it was a gram negative rod. The strain was tested for the production of extracellular lipase enzyme. The enzymes were partially purified by 90% saturated ammonium sulfate and dialysis for desalting. The bacterium was identified as Pseudomonas sp ADT3 by 16S rRNA amplification and sequencing which had been deposited in the NCBI GenBank with accession number JX914667. Phylogenetic tree was also constructed with MEGA5 software and showed the highest level of sequence similarity with Pseudomonas sp. HC3-13 strain. The microorganism had a growth optimum at pH 8.0 and temperature 22°C. Optimization of different parameters e.g. temperature, pH, incubation time, cofactors etc. was performed for the extracellular lipase activity. The hydrolytic activity of the enzyme was enhanced 5 times by Pb2+ but strongly inhibited by heavy metals Hg2+ as well as EDTA and β-mercaptoethanol. For the molecular weight estimation of enzyme SDS-PAGE was done which showed an inducible band of approximately 13.9 KDa. Activity staining and mass spectrometry techniques were also performed.

关键词： Psychrotrophic Arctic Extracellular Lipase 16S rRNA Mass Spectrometry

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