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Modeling gut neuro-epithelial connections in a novel microfluidic device

Microsystems & Nanoengineering 2023年第6期9卷 235-246页

作者： Jose M.de Hoyos-Vega Xi Yu Alan M.Gonzalez-Suarez Sisi Chen arnaldo mercado-perez Eugene Krueger Jeric Hernandez Yaroslav Fedyshyn Brooke R.Druliner David R.Linden Arthur Beyder Alexander Revzin Department of Physiology and Biomedical Engineering Mayo ClinicRochesterMNUSA Enteric Neuroscience Program(ENSP) Mayo ClinicRochesterMNUSA Division of Gastroenterology and Hepatology Department of MedicineMayo ClinicRochesterMNUSA

The intestinal lumen is filled with diverse chemical and physical stimuli.Intestinal epithelial cells sense these stimuli and signal to enteric neurons which coordinate a range of physiologic processes required for normal digestive tract function.Yet,the neuro-epithelial connections remain poorly resolved,in part because the tools for orchestrating interactions between these cellular compartments are lacking.We describe the development of a two-compartment microfluidic device for co-culturing enteric neurons with intestinal epithelial cells.The device contains epithelial and neuronal compartments connected by microgrooves.The epithelial compartment was designed for cell seeding via injection and confinement of intestinal epithelial cells derived from human intestinal organoids.We demonstrated that organoids planarized effectively and retained epithelial phenotype for over a week.In the second chamber we dissociated and cultured intestinal myenteric neurons including intrinsic primary afferent neurons(IPANs)from transgenic mice that expressed the fluorescent protein tdTomato.IPANs extended projections into microgrooves,surrounded and frequently made contacts with epithelial cells.The density and directionality of neuronal projections were enhanced by the presence of epithelial cells in the adjacent compartment.Our microfluidic device represents a platform that may,in the future,be used to dissect structure and function of neuro-epithelial connections in the gut and other organs(skin,lung,bladder,and others)in health and disease.

关键词： fluid bladder organs

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Candelilla Wax as Natural Slow-Release Matrix for Fertilizers Encapsulated by Spray Chilling

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Journal of Renewable Materials 2018年第3期6卷 226-236页

作者： Noe Navarro-Guajardo Edgar Miguel Garcia-Carrillo Carlos Espinoza-Gonzalez Ruben Tellez-Zablah Fatima Davila-Hernandez Jorge Romero-Garcia Antonio Ledezma-perez Jesus Alfonso mercado-Silva Claudia Anahi perez Torres Nicolaza Pariona Technological Institute of Saltillo Blvd.Venustiano Carranza 2400C.P.25280SaltilloCoahuilaMéxico Research Center for Applied Chemistry Sonomimetikos Research GroupBlvd.Enrique Reyna Hermosillo 140C.P.25294SaltilloCoahuilaMéxico Research Center for Applied Chemistry National Laboratory of GrapheneBlvd.Enrique Reyna Hermosillo 140C.P.25294SaltilloCoahuilaMéxico CONACyT–Institute of Ecology A.C. Carretera antigua a Coatepec 351El HayaC.P.91070XalapaVeracruzMéxico Network for Advanced Molecular Studies Institute of Ecology A.C.Carretera antigua a Coatepec 351El HayaC.P.91070XalapaVeracruzMéxico

Efforts to improve crop yields with efficient use of fertilizers are needed to guarantee global food security.Enhanced slow-release fertilizer systems(SRFs)encapsulated in biodegradable matrices are being developed to address this global concern.From a wide range of strategies for SRFs development,we explored a natureinspired solution based on the plant cuticle model and its function as a membrane for water and nutrient transport control.Here,Candelilla wax,extracted from Candelilla wild plants(Euphorbia antisyphilitica),is studied as a renewable slow-release matrix for fertilizers encapsulated by a modified spray chilling process.From this process,microencapsulates containing 40 wt%of phosphorus fertilizer are obtained with distinctive sizes and chemical characteristics,presenting a slow-release behavior.Considering the abovementioned features,novel insights into fertilizer release mechanisms based on plant cuticle models are discussed.

关键词： Candelilla wax encapsulation phosphorus slow-release fertilizers(SRFs) spray chilling

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Early Feeding vs 5-Day Fasting after Distal Elective Bowel Anastomoses in Children.A Randomized Controlled Trial

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Surgical Science 2013年第1期4卷 45-48页

作者： Roberto Davila-perez Eduardo Bracho-Blanchet Francisco Galindo-Rocha Jose Tovilla-mercado Gustavo Varela-Fascinetto Emilio Fernandez-Portilla Pablo Lezama-del-Valle Jaime Nieto-Zermeno Pediatric Surgery Department Hospital Infantil de Mexico Federico GomezMexico CityMexico

Purpose: Determine the safety and efficacy of early enteral feeding after distal elective bowel anastomoses (DEBA) in children. Methods: Controlled randomized trial including pediatric patients with DEBA, excluding non-elective and high risk patients. Variables: Demographics, operative time, anastomosis placement, beginning peristalsis and bowel movement, time to full diet intake, post-operative stay, persisting vomiting and abdominal distention, wound infection or dehiscence, anastomotic leak, reoperation, death. Randomization into: 1) Experimental group (EG): early feeding group, after a minimum 24 hours fasting period, oral fluids and diet was started;2) Control group (CG): obligatory 5-day fasting. Descriptive Statistics: Student’s t test for quantitative and Chi square for qualitative variables, a p-value 0.05 was considered significant. Results: 60 patients were included since June 2003 to May 2004, 30 ineach group. Mean age 2 years, weight16 kg, malnutrition 33%. Stomal Ethiology: Anorectal-malformation 46%, Hirschsprung 13%, inflammatory 35%, tumoral 5%. Mostly in colon 71%. Mean surgical time 142 min. None developed vomiting or required nasogastric-tube. Mild abdominal distension 13%, mild fever 16.5% and wound complications 18%. Anastomosis leakage 6.5%, none required reoperations. Demographic variables showed no statistical differences. Full oral intake was in the 2nd postoperative day in the EG vs CG (p = 0.001). Postoperative hospital stay was 6.0 ± 3 in the EG vs 9.8 ± 4 days in the CG with clinical but not statistical significance. Peristalsis beginning, first flatus passage and bowel movements showed no statistical differences. The complication incidence was low and equally distributed. Conclusions: Early feeding after DEBA is safe and well tolerated in children.

关键词： Early Feeding Bowel Anastomosis Children

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Hypoglycemic Therapy in Chronic Hepatic Disease Literature Review

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Open Journal of Endocrine and Metabolic Diseases 2020年第10期10卷 137-146页

作者： Pedro Luis Imbeth-Acosta Nehomar Pájaro-Galvis Víctor Leal-Martinez Jorge Rico-Fontalvo Rodrigo Daza-Arnedo Orlando Castañeda-López María Cardona-Blanco Karen mercado-Anillo José Lucas-Daza Christian perez-Calvo Jhonatan Hernández-Meza Álvaro Barraza-Pombo Marianela Florez-Ortega Jorge Hoyos-Fortich Isabella Uparella-Gulfo Blas de Lezo Clinic Cartagena Bolívar Colombia Faculty of Health Sciences University of Sinú Cartagena Bolívar Colombia New Bocagrande Hospital Cartagena Bolívar Colombia Colombian Association of Nephrology Medellín Colombia Colombian Association of Nephrology Cartagena Colombia Endocrinos SAS Cartagena Colombia Renal Unit B. Braun Medellín Antioquia Colombia University of Cartagena Cartagena Bolívar Colombia Colombian Association of Nephrology Ibagué Colombia Caribbean University Hospital Cartagena Colombia University of Sinu Cartagena Colombia Epidemiologist Blas de Lezo Clinic Cartagena Colombia Naval Hospital Cartagena Colombia Rafael Nuñ ez University Cartagena Colombia

Chronic liver disease (CLD) refers to a structural and functional change of the liver, which modifies the pharmacokinetics of multiple drugs, including hypoglycemic agents. This alteration depends on the severity degree of the liver disease, clinical characteristics of the patient, and comorbidities presence such as kidney disease and drug biochemistry. Insulin is considered a safe therapeutic strategy in patients with CLD, however, for many oral hypoglycemic agents, its use and dose adjustment will depend on the Child-Pugh score, based on the risk of hypoglycemia in this type of patient.

关键词： Cirrhosis Hypoglycemic Agents Pharmacokinetics (MeSH)

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