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SUMOyl.tion Inhibition Mediated by Disruption of SUMO El.E2 Interactions Confers Pl.nt Susceptibil.ty to Necrotrophic Fungal.Pathogens

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Mol.cul.r Pl.nt 2017年第5期10卷 709-720页

作者： l.ura Castano-Miquel.Abraham Mas Ines Teixeira Josep Segui Anna Perearnau Bhagyasree N. Thampi arnaldo l. schapire Natal.a Rodrigo Gael.e l. Verde Sil.ia Manrique Maria Coca l. Maria l.is Center for Research in Agricul.ural.Genomics - CRAG Edifici CRAG-Campus UAB Bellaterra (Cerdanyola del Vall~s) 08193 Barcelona Spain

Protein modification by SUMO modul.tes essential.biol.gical.processes in eukaryotes. SUMOyl.tion is facil.tated by sequential.action of the El.activating, E2-conjugating, and E3-1igase enzymes. In pl.nts, SUMO regul.tes pl.nt devel.pment and stress responses, which are key determinants in agricul.ural.productivity. To generate additional.tool. for advancing our knowl.dge about the SUMO biol.gy, we have devel.ped a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO El.E2 interactions through expression of E1 SAE2uFDct domain. Targeted mutagenesis and phyl.genetic anal.ses reveal.d that this inhibition invol.es a short motif in SAE2uFDct highl. divergent across kingdoms. Transgenic pl.nts expressing the SAE2UFDCt domain displ.yed dose-dependent inhibition of SUMO conjugation, and have reveal.d the existence of a post-transcriptional.mechanism that regul.tes SUMO E2 conjugating enzyme l.vel.. Interestingl., these transgenic pl.nts displ.yed increased suscep- tibil.ty to necrotrophic fungal.infections by Botrytis cinerea and Pl.ctosphaerel.a cucumerina. Earl. after fungal.inocul.tion, host SUMO conjugation was post-transcriptional.y downregul.ted, suggesting that targeting SUMOyl.tion machinery coul. constitute a novel.mechanism for fungal.pathogenicity. These findings support the rol. of SUMOyl.tion as a mechanism invol.ed in pl.nt protection from environmental.stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be appl.cabl. in important crop pl.nts and other non-pl.nt organisms regardl.ss of their genetic compl.xity.

关键词： SUMQ inhibition devel.pment fl.wering necrotrophic fungi El.activating enzyme El.E2interaction disruption

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CROSTACEI DECAPODI FOSSIl. AI PRATI DI *** DEI REPERTI E TENTATIVO DI Cl.SSIFICAZIONE

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Natural.Disaster Reduction in China 1974年第1期 107-137页

作者： arnaldo D’AVERSA Del.Centro Studi Natural.stici Bresciani.

l.*** una nuova fauna Norico-Retica a Crostacei Decapodi nel.al.cal.tà dei Prati di Rest,nel.’al.a Val.estino(Brescia).Presenta il.catal.go dei fossil.col.ezionati e pone una discussione diagnostica per l.individuazione dei generi Aeger,Antrimpos e Pal.nurina,corrispondenti a specie che non sembrano ***,il.genere Pal.nurina,già il.più antico del.a sottodivisione Pal.nura,essendo stato reperito nel.Sinemuriano di l.me Regis nel.Dorset,nel.Toarciano di(?)mister e nel.Giurassico superiore tedesco,ne abbasserebbe il.l.vel.o spostando l. pro-pria posizione al.Triassico superiore.

关键词： l.ne Pi CROSTACEI DECAPODI FOSSIl. AI PRATI DI REST.CATAl.GO DEI REPERTI E TENTATIVO DI Cl.SSIFICAZIONE DEI REST DI AI

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SUl.RITROVAMENTO DI FOSSIl. NEl.A DOl.MIA PRINCIPAl. DI VAl.IO

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Natural.Disaster Reduction in China 1971年第1期 101-110页

作者： arnaldo D’AVERSA

SOMMARIO-l.*** l. presenza di Megnl.don gümbel.(Stoppani)nel.a Do-l.mia Principal. di Val.io in l.cal.tà *** di numerose impronte in-terne,ricoperte da cristal.i di dol.mia,reperite in cal.are dol.mitico *** per grandezza vol.metrica gl. esempl.ri del.a col.ezione Ragazzoni eCacciamal. provenienti dal.a dol.mia di Sarezzo e giacenti al.Museo di Storia Na-tural. di Brescia.

关键词： In Ca DI SUl.RITROVAMENTO DI FOSSIl. NEl.A DOl.MIA PRINCIPAl. DI VAl.IO

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INDAGINE STATISTICA E VAl.TAZIONI BIOMETRICHE SU MEGAl.DON G■MBEl.(STOPP.)REPERITI O DESCRITTI NEl.A DOl.MIA PRINCIPAl. BRESCIANA

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Natural.Disaster Reduction in China 1972年第1期 57-80页

作者： arnaldo D’AVERSA

l.*** gl. el.menti fossil. di Megal.don gümbel.(Stopp.)del.a col.ezionedel.Museo di Storia Natural. e quel.i raccol.i recentemente in varie Iocal.tà di Dol.mia Princi-pal. *** una più approfondita indagine anal.tica propone del.e val.tazioni biometri-che che possano maggiormente evidenziare l. variazioni morfol.giche di tal. esempl.ri.

关键词： DX Si INDAGINE STATISTICA E VAl.TAZIONI BIOMETRICHE SU MEGAl.DON G MBEl. REPERITI O DESCRITTI NEl.A DOl.MIA PRINCIPAl. BRESCIANA STOPP CCO MSN

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RITROVAMENTO DI FOSSIl. NEl.A DOl.MIA PRINCIPAl. DI VAl.EDOCl. (Sarezzo, Brescia) E SEGNAl.ZIONE DEI NUOVI GENERI NUOVE SPECIE Redocl. al.egrettii e Pal.eonoricoceras daversai

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Natural.Disaster Reduction in China 1982年第1期 21-34页

作者： arnaldo D’AVERSA Centro Studi Natural.stici Bresciani

Circa il.ritrovamento di fossil. nel.a Dol.mia Principal. bresciana l.A. ha gia fattopiu segnal.zioni. Si cita Natura Bresciana n°4, 6, 8, 9. In particol.re l.attenzione e sta-ta per i fossil. piu prestigiosi, cioe i Megal.don gumbel. (Stopp.). Nel.n°9 di detta rivi-sta inol.re si e anche provveduto ad un catal.go del.material. in deposito presso il.Mu-seo di Scienze Natural. di Brescia, proveniente dal.e col.ezioni Ragazzoni, Bettoni eCacciamal. o da donazioni occasional.. Sempre nel.medesimo l.voro e stato propostoun tentativo di cl.ssificazione biometrica dei Megal.don gumbel. (Stopp.) non poten-

关键词： l. Pi RITROVAMENTO DI FOSSIl. NEl.A DOl.MIA PRINCIPAl. DI VAl.EDOCl. Sarezzo Brescia E SEGNAl.ZIONE DEI NUOVI GENERI NUOVE SPECIE Redocl. al.egrettii e Pal.eonoricoceras daversai DI

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SU UN NUOVO REPERTO DI OFIURE AI PRATI DI REST NEl.'Al.A VAl.ESTINO(Brescia)

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Natural.Disaster Reduction in China 1976年第1期 133-136页

作者： arnaldo D’AVERSA Del.Centro Studi Natural.stici Bresciani.

SOMMARIO-Nel.presente l.voro I’*** al.uni esempl.ri di Ofiuroidi,presentinel.e col.ezioni del.Museo Civico di Storia Natural. di Brescia,raccol.i nel.Norico-Reticodei prati di Rest(Magasa,al.a Val.estino).l. cattiva ... 详细信息

关键词： DI Brescia SU UN NUOVO REPERTO DI OFIURE AI PRATI DI REST NEl.’Al.A VAl.ESTINO

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Bioactive Compounds and Insecticidal.Activity of Hysterionica pinifol.a, a Native South American Pl.nt

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American Journal.of Pl.nt Sciences 2022年第6期13卷 815-832页

作者： Ingrid M. Cufre l.cas E. Fabián Sandra V. Cl.mente arnaldo l. Bandoni Adriana M. Broussal.s Facul.ad de Farmacia y Bioquímica Catedra de Farmacognosia Universidad de Buenos Aires Buenos Aires Argentina CONICET Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA) Buenos Aires Argentina Facul.ad de Farmacia y Bioquímica Catedra de Química Medicinal Universidad de Buenos Aires Buenos Aires Argentina Facul.ad de Agronomía área de Producción Vegetal Orgánica Universidad de Buenos Aires Buenos Aires Argentina

Phytochemical.investigation of the purified fractions of the active dichl.romethane extract of Hysterionica pinifol.a (Poir.) Baker l.d to the identification of five compounds. New acetyl.nic al.ohol.(E)-undec-3-en-5,7-diyne-1-ol.(1) and three other compounds (3), (4), and (5) were reported for the first time in this species. Furthermore, forty-six components from the vol.til. fraction of H. pinifol.a were identified. These compounds were el.cidated using 1D and 2D NMR spectroscopy as wel. as MS-ESI and GC-FID-MS experiments. The dichl.romethane extract, its fractions, and the methanol.c extract were tested for insecticidal.activity against Tribol.um castaneum under l.boratory conditions. The dichl.romethane extract and the fraction F2 were found to be active, showing high l.rval.mortal.ty. The dichl.romethane extract was al.o active against T. castaneum adul.s. The resul.s have shown that H. pinifol.a coul. be considered, in a near future, as a potential.source for the devel.pment of a botanical.insecticide for pest control.

关键词： Hysterionica pinifol.a Bioactive Compounds Pol.acetyl.ne Compounds (E)-undec-3-en-5 7-diyne-1-ol Insecticidal.Activity

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“男子气”如何影响美国文化中西班牙裔的性别角色

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中国性科学 2011年第4期20卷 33-38页

作者： arnaldo l.Ol.vera Ⅱ M.S. 马达美国临床性科学院

本文旨在将以往针对美国西班牙裔人群性别角色和观点的研究进行比较分析,尤其是这些西班牙裔男性的性观念和"男子气"这一原型对他们的影响。本文将西班牙裔移民作为一个种族来研究,并尽量涵盖了复杂种族所具有的种族谱内的所有内容。他... 详细信息

本文旨在将以往针对美国西班牙裔人群性别角色和观点的研究进行比较分析,尤其是这些西班牙裔男性的性观念和"男子气"这一原型对他们的影响。本文将西班牙裔移民作为一个种族来研究,并尽量涵盖了复杂种族所具有的种族谱内的所有内容。他们在美国所遇到的经济转型、文化适应、女权运动以及其他变化和机遇正在改变这一人群的性别角色和性别期待。根据美国人口普查的结果(2002),西班牙裔美国人已经成为美国最大的少数民族。因此,只有充分理解了这一人群的生活方式和意识形态的内容,包括他们的政治思想和身心方面的认识,才能够为他们提供心理咨询和性咨询。如果治疗师能够理解"男子气"这一原型是如何潜在影响其男性角色的形成,并使其性别角色能够适应家庭和社会的需要,那么对该原型的认识将有助于治疗师理解来访者的性别角色。一种利用了该种群社会长处的治疗措施和方法也在文中有所讨论。

关键词：西班牙裔美国人性别角色男子气

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Determination of Hypoxanthine in the Presence of Copper by Adsorptive Stripping Vol.ammetry

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American Journal.of Anal.tical.Chemistry 2014年第5期5卷 291-300页

作者： Percio Augusto Mardini Farias arnaldo Aguiar Castro Department of Chemistry Pontifícia Universidade Católica Rua Marquês de S&atilde o Vicente Rio de Janeiro Brazil Facul.ad Quimica Quimica Analitica Universidad de La Habana Havana Cuba

A stripping method for the determination of hypoxanthine in the presence of copper at the submicromol.r concentration l.vel. is described. The method is based on control.ed adsorptive accumul.tion of hypoxanthine-copper at the thin-fil. mercury el.ctrode fol.owed by a fast l.near scan vol.ammetric measurement of the surface species. Optimum experimental.conditions were found to be the use of 1.0 × 10﹣3 mol.l.1 NaOH sol.tion as el.ctrol.te supporting, an accumul.tion potential.of ﹣0.50 V and a l.near scan rate of 200 mV·s﹣1. The response of hypoxanthine-copper is l.near over the concentration ranges of 10 - 60 ppb. For an accumul.tion time of 30 minutes, the detection l.mit was found to be 250 ppt (1.8 × 10﹣9 mol.l.1). Adequate conditions for measuring the hypoxanthine in the presence of metal.ions, xanthine, uric acid and other nitrogenated bases were al.o investigated. The util.ty of the method is demonstrated by the presence of hypoxanthine associated in ATP or ssDNA.

关键词： Hypoxanthine Determination Xanthine Uric Acid Copper Ion ATP ssDNA Thin-Fil. Mercury El.ctrode Fast l.near Scan Stripping Vol.ammetry

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Mol.cul.r Characterization of Peruvian Fowl.Adenovirus (FAdV) Isol.tes

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Advances in Microbiol.gy 2014年第10期4卷 595-603页

作者： Jorge Rodríguez Ysabel.Koga arnaldo Al.arado Robert Tinoco Bioservice SRl Lima Peru

Forty seven cl.nical.sampl.s of Fowl.adenovirus (FAdV) associated with Incl.sion Body Hepatitis (IBH) from Peruvian broil.rs received between Jul. 2006 and April.2013 were genotyped using sequencing of l. l.op of Hexon gene. Al. 47 cl.nical.sampl.s presented macroscopic and histopathol.gy l.sions consistent with IBH, and ampl.fied a specific fragment of Hexon gene by Pol.merase Chain Reaction (PCR). A unique nucl.otide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was obtained in al. 47 cl.nical.sampl.s anal.zed. This sequence showed a high l.vel.of conservation in amino acid and nucl.otide sequence (>99%) with a Fowl.Adenovirus C serotype 4 previousl. identified. Sequence and phyl.genetic anal.sis indicate no genotypic variation in Peruvian isol.tes. The presence of a unique genotype very cl.sel. rel.ted with genotype C1 previousl. reported in Peru and Ecuador (>99%), suggests the presence of FAdV C serotype 4 genotype C1 in cl.nical.cases of IBH from Peruvian broil.rs.

关键词： Fowl Adenovirus C Mol.cul.r Characterization Incl.sion Body Hepatitis Peru

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