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High-fructose corn syrup production and its new applications for 5-hydroxymethylfurfural and value-added furan derivatives:Promises and challenges

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Journal of Bioresources and Bioproducts 2022年第3期7卷 148-160页

作者： aristide laurel mokale kognou Sarita Shrestha Zihua Jiang Chunbao(Charles)Xu Fubao Sun Wensheng Qin Department of Biology Lakehead UniversityThunder Bay P7B 5E1Canada Department of Chemistry Lakehead UniversityThunder Bay P7B 5E1Canada Department of Chemical and Biochemical Engineering Western UniversityLondon N6A 5B9Canada School of Biotechnology Jiangnan UniversityWuxi 214122China

High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial *** in enzyme engineering and commercial production of glucose isomerase have paved the way to explore more efficient variants of these *** 5-hydroxymethylfurfural can be produced from high fructose corn syrup catalytic dehydration,and it can be further converted into various furanic compounds chemically or biologically for various industrial applications as a promising platform *** the chemical conversion of 5-hydroxymethylfurfural into furanic compounds has been extensively investigated in recent years,bioconversion has shown promise for its mild conditions due to the harsh chemical reaction *** review discusses pro-tein engineering potential for improving glucose isomerase production and recent advancements in bioconversion of 5-hydroxymethylfurfural into value-added furanic *** suggests bi-ological strategies for the industrial transformation of 5-hydroxymethylfurfural.

关键词： High fructose corn syrup Glucose isomerase Rational enzyme engineering Directed evolution Furanic derivatives Biocatalysts

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Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass

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Green Chemical Engineering 2023年第2期4卷 239-249页

作者： aristide laurel mokale kognou Chonlong Chio Janak Raj Khatiwada Sarita Shrestha Xuantong Chen Hongwei Li Yuen Zhu Zi-Hua Jiang Chunbao(Charles)Xu Wensheng Qin Department of Biology Lakehead UniversityThunder BayONP7B 5E1Canada Department of Chemical and Biochemical Engineering Western UniversityLondonONN6A 5B9Canada School of Environment and Resources Shanxi UniversityTaiyuan030006China Department of Chemistry Lakehead UniversityThunder BayONP7B 5E1Canada

Glucose isomerase(GI)is an enzyme with high potential *** of GI producing bacteria with interesting properties from an industrial point of view is *** sp.,Paenarthrobacter sp.,Chryseobacterium sp.,Hymenobacter sp.,Mycobacterium sp.,and Stenotrophomonas *** isolated from soil *** of enzyme production yield was investigated in various fermentation conditions using response surface *** isolates exhibited maximum GI activity at 40℃,pH 6–8 after 4 days of incubation.A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme *** same trend was observed in fermentation medium containing 1%xylose or 2%–2.5%wheat *** study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry.

关键词： Cellulolytic bacteria Glucose/xylose isomerase 16S rRNA Biomass conversion

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Acute and Sub-Chronic Toxicity Evaluation of the Crude Methanolic Bark Extract of Bridelia micrantha (Hochst.) Baill. (Phyllanthaceae) and Its Fraction

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Journal of Biosciences and Medicines 2023年第10期11卷 76-89页

作者： Colette Elysée Aboudi Etono Landry Lienou Lienou Fabrice Fabien Dongho Dongmo aristide laurel mokale kognou Raphael Tchientcheu Rebecca Madeleine Ebelle Etame Rosalie Anne Ngono Ngane Laboratory of Biochemistry Faculty of Science University of Douala Douala Cameroon Centre for Research on Medicinal Plants and Traditional Medicine Institute of Medical Research and Medicinal Plants Studies Ministry of Scientific Yaounde Cameroon

Bridelia micrantha, commonly known as coastal golden leaf, is a member of the family Phyllanthaceae. In preliminary studies nine fractions, named F1 - F9, were obtained by fractionating the crude methanol extract of the stem bark of Bridelia micrantha using column chromatographic techniques. The fraction F6 was the most active when tested for antibacterial activity. Thus, toxicity of this fraction was investigated for further use. The present study evaluated the acute and sub-chronic toxicity of the crude methanolic bark extract of Bridelia micrantha and its fraction. The acute toxicity was carried out according to the experimental protocol of Organization for Economic Co-operation and Development (OECD). The plant extract or the fraction F6 was administered orally to female mice at a single dose of 2000 mg/kg and the animals were observed for any behavioral changes or mortality for 14 days. In the sub-chronic toxicity study, the extract and fraction were administered orally at 200, 400 and 800 mg/kg bw/day for 28 days to healthy Wistar rats. The general behavior and body weight of the rats were recorded daily. At the end of the experimental period, hematological and biochemical analyses, changes in vital organ weight (liver, lung, heart, spleen and kidney), and histopathological examination of the liver and kidney were performed. No mortality or adverse effects were noted at the 2000 mg/kg dose during the oral acute toxicity test. In the sub-chronic study, the crude methanolic bark extract of Bridelia micrantha and the fraction F6 induced no mortality or treatment-related adverse effects on body weight, general behavior, relative organ weights, hematological and biochemical parameters. Histopathological examination of the liver and kidney showed normal architecture suggesting no morphological alterations. In conclusion, the oral administration of the crude methanolic bark extract of Bridelia micrantha and the fraction F6 for 28 days

关键词： Bridelia micrantha Acute Toxicity Sub-Chronic Toxicity Biochemical Parameters Hematological Parameters

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