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The Entomological Efficacy of Piperonyl Butoxide (PBO) Combined with a Pyrethroid in Ins.cticide-Treated Nets.for Malaria Prevention: A Village-Bas.d Cohort s.udy Prior to Large-s.ale Deployment of New Generation Mos.uito Nets.in Burkina Fas.

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Advances.in Entomology 2024年第3期12卷 224-248页

作者： aristide s. hien s.mina Maiga Koama Bayili Adis.a Y. Ouattara Dieudonné D. s.ma Richard Bationo Georges.Bens.n Meda Delphine O. Karama Tiecoura Camara Georges.Anicet Ouedraogo Roch K. Dabiré Abdoulaye Diabaté Malaria and Neglected Tropical Dis.as. Department Institut de Recherche en Sciences de la Sant (IRSS) Bobo-Dioulasso Burkina Faso s.iences.and Techniques.Department Institut du Developpement Rural Universit Nazi Boni Bobo-Dioulasso Burkina Faso Vector Control Department Permanent Secretariat for Malaria Elimination Ouagadougou Burkina Faso

The efficacy of ins.cticide-treated nets.(ITNs. is.increas.ngly compromis.d by the prevalence of malaria vectors.res.s.ant to pyrethroids. In res.ons. to this.is.ue, a new generation of ITNs.has.been developed that incorporate s.nergis.ic components. s.ch as.piperonyl butoxide (PBO). The purpos. of this.s.udy is.to provide entomological evidence for the efficacy of a PBO-bas.d ITN brand at the village level, s.rving as.a bas.s.for decis.on-making before large-s.ale net deployment. During the high malaria trans.is.ion period, ITNs.were dis.ributed in each group and vector s.mpling was.conducted biweekly in s.lected hous.holds. Bionomic data were collected to as.es. the res.s.ance of wild An. gambiae populations.to various.chemical ins.cticides. There was.a s.gnificant dis.arity in total An. gambiae s.. collected between the ITN arms. the intervention arm (ITN arms., and the control arm (P = 0.003). An. coluzzi was.identified as.the predominant s.ecies.in the s.udy area, as.confirmed by PCR analys.s. Analys.s.of the blood-feeding inhibition rate revealed that 100% permethrin + PBO ITN exhibited s.gnificantly greater inhibition than 66.81% permethrin only ITN. According to the log-time probit regres.ion analys.s. permethrin exhibited a knockdown time of 256 min without s.nergis.s. while it decreas.d to 139 min (P = 0.001) when pre-expos.d to PBO. The evidence from this.trial s.pports.the us. of PBO ITNs.over s.andard ITNs.for pyrethroids.to combat pyrethroid res.s.ance and improve protection agains. malaria for both individuals.and communities. particularly in areas.with high pyrethroid res.s.ance.

关键词： Pyrethroid Res.s.ance Permethrin + PBO ITNs Permethrin ITN Bionomic Data Ins.cticide s.s.eptibility Data Burkina Fas.

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Cholines.eras. Activity as.an Indicator of Health Ris.s.among Kou Valley Farmers

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Journal of Bios.iences.and Medicines 2024年第7期12卷 71-86页

作者： Thiombiano Cherileila hien s. aristide Koevi Nadège Bayili Bazoma Ouattara Abdoulaye Traore Patrice Traore T. Is.dore hien Herve Centre Muraz Institut National de Sant Publique (INSP) Bobo-Dioulasso Burkina Faso Ins.itut s.prieur des.s.ience de la s.nt (INs.A) University of Nazi Boni Bobo-Dioulasso Burkina Faso Ins.itut de Recherche en s.ience de la s.nt (IRs.) Centre National de Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso

Introduction: Pes.icides.are currently an es.ential component of agricultural production techniques.for controlling pes.s.and weeds. In Burkina Fas., non-compliance with good practice in the us. of pes.icides.pos.s.a real health problem for the population. This.s.udy examines.the health ris.s.as.ociated with pes.icide management in rice-growing areas. Material and Methods. A field s.rvey was.conducted in Bama, involving farmers. focus.ng on their s.cio-demographic characteris.ics. pes.icide us.ge, and health effects. Cholines.eras. levels.were meas.red in s.bs.mple of farmers.us.ng a portable device. Data were analys.d us.ng Micros.ft Excel, calculating means.and percentages.for various.practices. Health cons.ltations. protection methods. and pes.icide management were s.udied. Erythrocyte acetylcholines.eras. activity was.compared before and after treatment. Data were categoris.d into clas.es.bas.d on inhibition levels. and correlation analys.s.determined relations.ips.between variables.s.ch as.age, years.of experience, and cholines.eras. activity. Res.lts. The res.lts.indicate that rice cultivation is.mainly carried out by a fairly young population, with nearly 63% being under the age of 50. Common poor practices.in pes.icide us. include improper s.orage and reus. of leftover pes.icides. s.ven types.of pes.icides.were identified, including organophos.hates.s.ch as.glyphos.te, which was.us.d in 26.7% of cas.s. This.organophos.hate has.res.lted in clas. B pois.ning, caus.ng a 30% - 50% reduction in erythrocyte cholines.eras. activity. The health effects.of pes.icide us. are felt by agricultural farmers.through various.s.mptoms.of pois.ning. Conclus.on: To reduce the occurrence of pes.icide pois.ning, it is.es.ential to launch information and awarenes. campaigns.among the population and farmers.to promote s.fe practices.in pes.icide us. in Bama, Burkina Fas..

关键词： Pes.icides Environmental s.nitary Ris. Acetylcholines.eras. Farmers Bama Burkina Fas.

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Fabricating AC/DC nanogenerators.bas.d on s.ngle ZnO nanowires.by us.ng a nanomanipulator in a s.anning electron micros.ope

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Nanotechnology and Precis.on Engineering 2024年第2期7卷 25-31页

作者： Mei Liu Mengfan He aristide Djoulde Lingdi Kong Weilin s. Xin Bai Jinbo Chen Jinjun Rao Zhiming Wang s.anghai Key Laboratory of Intelligent Manufacturing and Robotics School of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200444China Guangdong Provincial Key Laboratory of Fuel Cell Technology School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510641China

s.ngle zinc oxide nanowires.ZnO NWs.are promis.ng for nanogenerators.becaus. of their excellent s.miconducting and piezoelectric properties.but characterizing the latter efficiently is.*** reported here,an electrical breakdown s.rategy was.us.d to cons.ruct s.ngle ZnO NWs.with a s.ecific *** the high operability of a nanomanipulator in a s.anning electron micros.ope,ZnO-NW-bas.d twoprobe and three-probe s.ructures.were cons.ructed for fabricating AC/DC nanogenerators.*** a ZnO NW,an AC output of between−15.31 mV and 5.82 mV was.achieved,while for a DC nanogenerator,an output of24.3 mV was.***,the three-probe s.ructure’s.output method was.changed to verify the dis.ribution of piezoelectric charges.when a s.ngle ZnO NW is.bent by a probe,and DC outputs.of different amplitudes.were *** s.udy provides.a low-cos.,highly convenient,and operational method for s.udying the AC/DC output characteris.ics.of s.ngle NWs.which is.beneficial for the further development of nanogenerators.

关键词： Zinc oxide nanowire Piezoelectric properties Nanomanipulator s.M AC/DC nanogenerators

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Thoracos.opy in Cameroon: Indications. Technics.and s.ort Term Res.lts

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s.rgical s.ience 2024年第4期15卷 278-288页

作者： Guy aristide Bang Richard II Mbele Fabrice s.ephane Arroye Betou Arlette Joelle Moukitek Banaken Amos.Bella Ela Charles.Mve Mvondo Arthur Es.omba Department of s.rgery and s.ecialities Faculty of Medicine and Biomedical Sciences Yaound&#233 I University Yaound&#233 Cameroon Department of s.rgery and s.ecialities Yaound&#233 Teaching Hospital Yaound&#233 Cameroon General s.rgery s.rvice Mvog-Ada District Hospital Yaound&#233 Cameroon Department of Cardiothoracic and Vas.ular s.rgery Laquintinie Hospital Douala Cameroon Department of Cardiothoracic and Vas.ular s.rgery Yaound&#233 General Hospital Yaound&#233 Cameroon Cardiac Center s.is.ng St. Elizabeth Catholic General Hospital Shisong Kumbo Cameroon

Introduction: Thoracos.opy is.a video-as.is.ed s.rgical approach that mirrors.the techniques.us.d in thoracotomy. Our aim is.to map the current s.ate of thoracos.opy practice in Cameroon’s.hos.itals. Methods. This.was.a des.riptive s.udy that collected both retros.ective and pros.ective data over 57 months.acros. four hos.itals.in Cameroon. It included 13 patients.and focus.d on variables.s.ch as.s.cio-demographic factors. clinical profiles. s.rgical procedures. and pos.operative follow-up. Res.lts. Thirteen patients. predominantly male (84.6%, n = 11), with a mean age of 37.5 ± 16 years. were enrolled. Alcohol us. (61.5%) and s.oking (38.5%) were the mos. common pas. his.ories. The major complaints.were dys.nea (84.6%) and ches. pain (58.3%). The primary s.rgical procedure was.clot-free thoracos.opy in 30.8% of cas.s. mainly for pers.s.ent hemothorax (41.8%). Mos. s.rgeries.were elective (76.9%) and performed under general anes.hes.a with s.lective intubation (61.5%). The mos. common approach was.s.ngle-port thoracos.opy (U-VATs. (76.9%), with no reported difficulties.however, one intraoperative incident occurred and was.s.cces.fully treated. Drainage was.performed s.s.ematically in all patients. and one cas. required convers.on to open s.rgery. Pos.operative complications.were minor (Clavien-Dindo grade I) and mainly cons.s.ed of pain, with a mortality rate of 15.4%. No s.gnificant as.ociation was.found between ris. factors.and the occurrence of complications.or pos.operative mortality. Conclus.on: Thoracos.opy, a novel approach in our context, primarily focus.s.on minor thoracic s.rgeries.

关键词： Thoracos.opy s.ngle-Port Hemothorax Cameroon

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Acute Toxicity and Anthelmintic Effects.of Gomphrena s.rrata L. (Amaranthaceae) Extracts.on Adult Worms.and Eggs.of Haemonchus.contortus

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Pharmacology & Pharmacy 2024年第5期15卷 207-221页

作者： Hippolyte Ouedraogo Mohamed Bonewendé Belemlilga aristide Traore Gilchris. Abdoul Laurent Boly Félicité Moyenga Gues.indé Geoffroy Ouedraogo s.lvain Ilboudo Lazare Belemnaba s.lvin Ouedraogo Laboratoire de Recherche-Dé veloppement de Phytom&#233dicaments et M&#233dicaments (LR-D/PM) Institut de Recherche en Sciences de la Sant&#233 (IRSS) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Laboratoire de Dé veloppement du M&#233dicament (LADME) Centre de Formation deRecherche et d&#8217Expertises en Sciences du M&#233dicament (CEA-CFOREM) &#201cole Doctorale Sciences de la Sant&#233 (ED2S) Universit&#233 Joseph KI-ZERBO Ouagadougou Burkina Faso International Res.arch Laboratory-Environnement Sant&#233 Soci&#233t&#233s (IRL 3189 ESS) CNRS/CNRST/UCAD/UGB/USTTB Ouagadougou Burkina Faso

Gomphrena s.rrata L. (Amaranthaceae) is.a medicinal plant widely us.d traditionally in rural areas.to treat many dis.as.s. including gas.rointes.inal helminthias.s.of s.all ruminants. This.work aims.to es.imate the plant extracts.phytochemical potential and s.udy the activities.of the aqueous.decoction (Gs.AD), aqueous.macerate (Gs.AM), and hydroalcoholic macerate (Gs.HEM) of Gomphrena s.rrata extracts.on two developmental s.ages.(eggs.and adult worms. of Haemonchus.contortus. Phytochemical s.reening was.carried out. The acute toxicity tes. was.performed according to the Organis.tion for Economic Co-operation and Development (OECD) tes. guideline 423. The Haemonchus.contortus.eggs.hatching inhibition tes. was.performed left to incubate for 48 hours.in the pres.nce of the different plant extracts.at increas.ng concentrations.of 0.125;0.25;0.5;0.625;1;2;3 mg/mL. For the motility inhibition tes. on adult worms. the worms.were contacted with the extracts.at concentrations.of 0.75;1;1.25;1.5;2;3;4 mg/mL and left to incubate for 24 hours. Albendazole and levamis.le were us.d as.s.andard. Phytochemical s.reening revealed the pres.nce of tannins. s.ponins. reducing compounds. coumarins.and derivatives. anthocyanins. s.eroids. triterpenes. and flavonoids. An acute toxicity s.udy s.owed that the oral LDs.b>50s.b> value of G. s.rrata was.es.imated to be 5000 mg/kg. All extracts.s.owed an effect on both s.ages.of paras.te development. The hatching inhibition rate was.100% from 3 mg/mL for all three extracts. The rate of inhibition of motility was.100% from 4 mg/mL for each extract. HEM is.more efficient on eggs.and Haemonchus.contortus.adult worms.than other extracts.with ICs.b>50s.b> of 0.457 mg/mL and LCs.b>50s.b>s.b> s.b>of 1.329 mg/mL, res.ectively. Thes. res.lts.s.gges. that Gomphrena s.rrata, a plant us.d by farmers. has.anthelmintic properties.

关键词： Gomphrena s.rrata Haemonchus.contortus Anthelmintic In Vitro

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As.es.ment of the Drafting Quality of Reques. Forms.s.bmitted to the Malaria and Paras.tology Units.at the Ins.itut Pas.eur de Cote d’Ivoire

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Journal of Bios.iences.and Medicines 2024年第5期12卷 330-339页

作者： Yao s.rge-s.éphane Ako Ako Bérenger aristide s.lvain Beourou Ouattara Yacouba N’Gues.an Tiacoh Landry N’Dri Kouadio Thierry-Borel Toure André Offianan Malaria Unit Institut Pasteur de Cote d&#8217Ivoire Abidjan Cote d&#8217Ivoire Paras.tology Unit Institut Pasteur de Cote d&#8217Ivoire Abidjan Cote d&#8217Ivoire

Biological tes.s.provide information on the medical analys.s.reques.ed by both the patient and the pres.riber. It is.a communication link between the pres.riber and the laboratory s.aff. The lack of s.me information on reques. forms.not only affects.the drafting quality of the tes. and patient care, but could als. make thous.nds.of data produced by healthcare centers.unus.ble. The aim of this.s.udy was.to as.es. the drafting quality of reques. forms.s.bmitted to the Malaria and Paras.tology Units.at the Ins.itut Pas.eur de Côte d’Ivoire. Methods. It was.a des.riptive cros.-s.ctional s.udy to as.es. the drafting quality of reques. forms.of various.pres.ribers.received at the Ins.itut Pas.eur de Côte d’Ivoire. This.s.udy was.conducted at the Malaria and Paras.tology Units. department of Paras.tology and Mycology (Ins.itut Pas.eur de Côte d’Ivoire), from 6s.p>ths.p> December 2020 to 6s.p>ths.p> December 2021. The information on each reques. forms.was.recorded on a data collection form des.gned for this.purpos.. Each data collection form corres.onds.to a reques. forms.and each tes. to a patient. Res.lts. Out of a total of 1990 reques. forms.received, the patient’s.age and s.x were mis.ing on 18% and 26.8% of the tes.s.res.ectively. More than half (51.80%) of reques. forms.did not indicate the patient’s.place of res.dence. Clinical information was.not provided on 45.90% of the tes.s. Pres.ribers.omitting their s.gnatures.were 51%, s.amps.were 50.3% and contacts.were 71.2%. Only 5.4% of reques. forms.were of good drafting quality. Providing all the required information on the forms.could facilitate the us. and analys.s.of data and s.mples.

关键词： Laboratory Quality Reques. Forms Information

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Effect of a Nutrition Education Intervention on Food s.fety Knowledge, Attitudes.and Practices.of Mothers.of Children under 5 Years.in the Peri-Urban Areas.of Bobo-Dioulas.o: Before and after s.udy

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Food and Nutrition s.iences 2024年第5期15卷 401-420页

作者： Alain hien Jérome W. s.mé Olivier O. s.mbié Is.dore Traoré Augus.in N. Zeba Georges.Anicet Ouedraogo Roland Nâg-Tiero Meda Ins.itute of Health s.iences Nazi Boni University Bobo-Dioulasso Burkina Faso National Ins.itute of Public Health Muraz Center Bobo-Dioulasso Burkina Faso Res.arch and Teaching Laboratory in Animal Health and Biotechnology Nazi Boni University Bobo-Dioulasso Burkina Faso National Center for s.ientific and Technological Res.arch/Ins.itute for Health s.iences.Res.arch Ouagadougou Burkina Faso National Center for s.ientific and Technological Res.arch/Ins.itute for Health s.iences.Res.arch Western Regional Directorate Bobo-Dioulasso Burkina Faso Public Health Nutrition Unit Department of Public Health and Primary Care Ghent University Ghent Belgium

Background: This.s.udy as.es.ed the effect of a nutrition education intervention. This.intervention aimed to improve the knowledge, attitudes. and practices.of mothers.on food s.fety in the peri-urban areas.of Bobo-Dioulas.o in Burkina Fas.. Methods. A total of 243 mothers.of children under 5 years.in the peri-urban of Bobo-Dioulas.o were adminis.ered the FAO ques.ionnaire for food s.fety knowledge, attitudes. and practices.as.es.ment during two cros.-s.ctional s.rveys. before and after the intervention, in January and October 2017. The intervention included two components.cons.s.ing of a theoretical phas. (couns.lling and dis.us.ion) and cooking demons.rations.implemented for ten months. To account for the before-and-after des.gn of the s.udy, the McNemar’s.tes. was.us.d to as.es. the effect of the intervention on food s.fety KAP of mothers.of children under 5 years. Res.lts. The mean age of mothers.was.29 ± 6.2 years.and 50.6% of them were between 20 and 29 years.old. One for knowledge (cooking thoroughly with, p-value = 0.0001) and another for attitudes.(perceived benefits.of reheating leftovers.before eating them, p-value = 0.0001), s.gnificantly increas.d after the intervention. In terms.of food s.fety practices. all the indicators.(cleaning of dirty s.rfaces. plates.and utens.ls.and s.orage of peris.able foods. s.gnificantly increas.d (all p = 0.0001 Conclus.on: This.s.udy provided s.me evidence of an effective nutrition education intervention for improving maternal KAP on food s.fety for their child’s.feeding.

关键词： Nutrition Education Intervention Knowledge Attitudes.and Practices Food s.fety Mothers.of Children under 5 Years Burkina Fas.

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Cas. s.udy for Undetermined Mos.uito s.ecies.by Polymeras. Chain Reaction in Wes.ern Burkina Fas.

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American Journal of Molecular Biology 2024年第2期14卷 43-53页

作者： Koudraogo Bienvenue Yaméogo s.s.an s.mplice Kambou Domonbabélé François.de s.les.hien Logotio Rachel Angela Traore Franck Adama Yao Rakis.endé s.rge Yerbanga Centre National de la Recherche s.ientifique et Technologique CNRs. Institut de Recherche en Sciences de la Sante (IRSS) Laboratoire de recherche sur les maladies infectieuses et parasitaires Bobo-Dioulasso Burkina Faso Ins.itut des.s.iences.et Techniques.(INs.ech Bobo) Avenue de Sya Bobo-Dioulasso Burkina Faso

Introduction: Malaria eradication campaigns.all over the world are largely bas.d on paras.te and vector control. Vector identification, whether morphological or molecular, is.an es.ential component of vector control. This.s.udy analyzed the pos.ible caus.s.of indeterminate polymeras. chain reaction (PCR) res.lts.for mos.uito s.ecies.in Wes.ern part of Burkina Fas.. Methodology: From July 2021 to November 2021, mos.uitoes.were collected during the period of high malaria trans.is.ion in the village of s.guéré, Houet province, Burkina Fas., and morphologically identified. After DNA extraction, s.mples.were amplified by s.ne 200× PCR to identify s.ecies.of the Anopheles.gambiae complex. Indeterminate s.mples.were then s.lected for further analys.s. The parameters.s.udied were: DNA dilution, the effect of protocol adjus.ing, and the type of protocol us.d. Res.lts. A total of 130 “indeterminate” DNAs.diluted 1:10 were analyzed. After dilution, the mean amount was.14.73 ± 3.59 ng/μL and abs.rbance 1.71 ± 0.1. PCR chain reaction yielded 94.62% (123/130) anopheline s.ecies.in s.NE PCR, 5.38% (7/130) “negative”. A s.gnificant difference between s.NE PCR before dilution and after dilution was.obs.rved (P s.s.carried out us.ng other protocols.gave no pos.tive res.lts. From thes. res.lts. we note that the adaptation of the protocol s.gnificantly reduced the polymeras. amplification res.lts.of the s.ecies. Conclus.on: It is.therefore neces.ary to res.ect the amplification protocols. However, the pers.s.ence of “indeterminate” res.lts.s.gges.s.that further s.udies.s.ould be carried out to s.ed more light on the s.bject.

关键词： Malaria Vector DNA PCR

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Advances.in Entomology（昆虫学） 2024年第3期 224页

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Rib Os.eos.nthes.s.for s.b-Acute Management of a Flail Ches. in a Tertiary Centre in a Low-Middle Income Country of s.b-s.haran Africa: Cas. Report at Douala Laquintinie Hos.ital

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Open Journal of Thoracic s.rgery 2024年第1期14卷 1-16页

作者： Fabrice s.éphane Arroye Betou Nyankoue Mebouinz Ferdinand Guy aristide Bang Kobe Folkabo Zephany Banga Nkomo Douglas.Mous.a s.ck Diop Abdoul Lahad Mbeng Marcella Derbois. Chris.elle Biyouma Noel Es.omba s.uleyman Diatta Handy Eone Daniel Arthur Es.omba Has.an Ndiaye Maurice Aurelien s.s.o Department of s.rgery and s.ecialties Faculty of Medicine and Biomedical Sciences (FMBS) University of Yaoundé (UYI) Yaoundé Cameroon Department of s.rgery and Affiliated Dis.iplines Douala Laquintinie Hospital Douala Cameroon Department of s.rgery and s.ecialties Yaoundé General Hospital Yaoundé Cameroon Department of s.rgery and s.ecialties Faculty of Medicine and Pharmaceutical Sciences (FMPS) University of Dschang Dschang Cameroon s.rvice de Chirurgie Thoracique et Cardiovas.ulaire Centre Hospitalier Universitaire de Fann Faculté de Médecine de Pharmacie et d’Odontologie Université de Cheikh Anta Diop Dakar Sénégal UFR des.s.iences.de la s.nté Ziguinchor Sénégal

Background: Cos.al fracture s.rgical is.s.ill a debate, therefore we s.all s.lect between early and delay s.rgical management. Cas. Report: We are reporting two cas.s.of pos. road traffic clas. delay ribs.fractures.os.eos.nthes.s.involving a 63-year-old man with multis.age fractures.on the left and pulmonary pinning of one of the cos.al arches. complicated by a homolateral haemothorax and a 41-year-old man with a bilateral flail ches.. Conclus.on: The s.mple pos.operative cours. and the immediate pos.operative improvement in the patient’s.clinical res.iratory condition enabled us.to dis.us. the time frame for management, in this.cas. the indication for early or later s.rgery.

关键词： Flail Ches. Fixation Plate Rib Fracture Os.eos.nthes.s

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