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Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1027-1032页

作者： Yosephine Sri Wulan Manuhara Alfinda Novi Kristanti Edy Setiti Wida Utami arif yachya Laboratory of Plant Tissue Culture Biology Department Faculty of Science and Technology Airlangga University Laboratory of Organic Chemistry Chemistry Department Faculty of Science and Technology Airlangga University Laboratory of Plant Tissue Culture Biology Department Faculty of Mathematics and Natural Sciences PGRI Adi Buana University

Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(***)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of *** were infected by Agrobacterium rhizogenes strain *** hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L *** BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for *** and dry weights of hairy roots at the end of culture were ***:Various concentrations of sucrose influenced the biomass accumulation of hairy *** biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than *** supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than ***,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of ***:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of *** in the large scale.

关键词： Talinum paniculatum Gaertn. Saponin Hairy root Liq

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