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Journal of Forestry Research 2015年第3期26卷 711-718页

作者： Marcelo Luiz Laia Acelino Couto Alfenas Sergio Hermínio Brommonschenkel Shinitiro Oda Eduardo José de Melo Inaê Mariêde Araú jo Silva Janaína Fernandes Gonalves ariadne marques Departamento de Engenharia Florestal Universidade Federal dos Vales do Jequitinhonha e Mucuri DiamantinaMinas Gerais 39100-000 Brazil Departamento de Fitopatologia Universidade Federal de Viosa Viosa Minas Gerais 36570-900 Brazil Suzano Bahia Sul Celulose S/A Suzano So Paulo Brazil

Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.

关键词： Cloning Conserved domains RGAs Rust Eucalyptus

Implication of gut microbiome in immunotherapy for colorectal cancer

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World Journal of Gastrointestinal Oncology 2022年第9期14卷 1665-1674页

作者： Evangelos Koustas Eleni-Myrto Trifylli Panagiotis Sarantis Nikolaos Papadopoulos Georgios Aloizos ariadne Tsagarakis Christos Damaskos Nikolaos Garmpis Anna Garmpi Athanasios G Papavassiliou Michalis V Karamouzis Department of Biological Chemistry Medical SchoolNational and Kapodistrian University of AthensAthens 11527Greece 1st Department of Internal Medicine 417 Army Share Fund Hospital of AthensAthens 11521AtticaGreece Beth Israel Deaconess Medical Center BostonMA 02215United States N.S.Christeas Laboratory of Experimental Surgery and Surgical Research Medical SchoolNational and Kapodistrian University of AthensAthens 11527Greece Second Department of Propedeutic Surgery Laiko General HospitalMedical SchoolNational and Kapodistrian University of AthensAthens 11527Greece First Department of Pathology Medical SchoolNational and Kapodistrian University of AthensAthens 11527Greece

Colorectal cancer(CRC) constitutes the third most frequently reported malignancy in the male population and the second most common in women in the last two decades. Colon carcinogenesis is a complex, multifactorial event, resulting from genetic and epigenetic aberrations, the impact of environmental factors, as well as the disturbance of the gut microbial ecosystem. The relationship between the intestinal microbiome and carcinogenesis was relatively undervalued in the last decade. However, its remarkable effect on metabolic and immune functions on the host has been in the spotlight as of recent years. There is a strong relationship between gut microbiome dysbiosis, bowel pathogenicity and responsiveness to anti-cancer treatment;including immunotherapy. Modifications of bacteriome consistency are closely associated with the immunologic response to immunotherapeutic agents. This condition that implies the necessity of gut microbiome manipulation. Thus, creatingan optimal response for CRC patients to immunotherapeutic agents. In this paper, we will review the current literature observing how gut microbiota influence the response of immunotherapy on CRC patients.

关键词： Colorectal cancer Gut microbiome Immunotherapy Checkpoint inhibitors Tumor micro-environment

Characterization and electrochemical performance of CeO2 and Eu-doped CeO2 films as a manganese redox flow battery component

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Journal of Rare Earths 2018年第10期36卷 1074-1083页

作者： Monica A.Rodrigues ariadne C.Catto Elson Longo Edson Nossol Renata C.Lima Institute of Chemistry.Federal University of Uberlandia 38400-902UberlandiaMGBrazil Institute of Chemistry Sao Paulo State UniversityP.O.Box 35514801-907Araraquara.SPBrazil LIEC Department of ChemistryFederal University of Sao Carlos13565-905Sao CarlosSPBrazil

Hexagonal CeO2 and Eu-doped CeO2 nanoparticles were obtained using a facile microwave-hydro thermal method under mild conditions and their application towards manganese redox flow battery component were studied. The structural properties were studied by X-ray diffraction and indicate that samples present a fluorite structure. Raman spectroscopy shows Eu3＋ ions substitute Ce4＋ and generate oxygen vacancies. Electrochemical properties of pure and Eu-doped CeO2 films deposited at graphite substrates investigated by cyclic voltammetry and galvanostatic charge-discharge indicate that dopant concentration affects the electrochemical properties of CeO2. The increase in the reversibility redox of electrochemical systems observed is attributed to coexistence of Ce4＋/Ce3＋ redox couple confirmed by ***-discharge tests display coulombic and voltage efficiency values of above 80% and 90%, respectively. The obtained specific capacity for Ce（0.99）Eu（0.01）O2（372.49 mAh/g） and pure oxide（334.84 mAh/g）indicates that both samples are promising for application in Mn-batteries.

关键词： Cerium oxide Europium Nanoparticles Electrochemical properties Batteries Rare earths

Large endoscopic mucosal resection for colorectal tumors exceeding 4 cm

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World Journal of Gastroenterology 2010年第5期16卷 588-595页

作者： Philippe Ah Soune Charles Ménard Ezzedine Salah ariadne Desjeux Jean-Charles Grimaud Marc Barthet Department of Gastroenterology Hpital NordChemin des Bourrely13915 Marseille cedex 20France

AIM:To evaluate the feasibility and the outcome of endoscopic mucosal resection(EMR)for large colorectal tumors exceeding 4 cm(LCRT)undergoing piecemeal resection. METHODS:From January 2005 to April 2008,146 digestive tumors larger than 2 cm were removed with the EMR technique in our *** these,34 tumors were larger than 4 cm and piecemeal resection was carried out on 26 colorectal *** mean age of the patients was 71 *** mean follow-up duration was 12 mo. RESULTS:LCRTs were located in the rectum,left colon,transverse colon and right colon in 58%,15%, 4%and 23%of cases,*** were sessile tumors larger than 4 cm with a mean size of 4.9 cm (4-10 cm).According to the Paris classification,34%of the tumors were typeⅠs,58%typeⅡa,4%typeⅡb and 4%typeⅡ*** examination showed tubulous adenoma in 31%,tubulo-villous adenoma in 27%,villous adenoma in 42%,high-grade dysplasia in38%,in situ carcinoma in 19%of the cases and mucosal carcinoma(m2)in 8%of the *** two cases(7.7%)of procedural bleeding that occurred were managed endoscopically and one small perforation was treated with *** follow-up,recurrence of the tumor occurred in three patients(12%),three of whom received endoscopic treatment. CONCLUSION:EMR for tumors larger than 4 cm is a safe and effective procedure that could compete with endoscopic submucosal dissection,despite providing incomplete histological assessment.

关键词： Endoscopic mucosal resection Perforation Colorectal carcinoma Large polyps

Critical Thinking in Language Learning and Teaching

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History Research 2017年第2期7卷 73-77页

作者： ariadne de Villa Texas Lutheran University

Increased branching independent of strigolactone in cytokinin oxidase 2-overexpressing tomato is mediated by reduced auxin transport

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Molecular Horticulture 2022年第1期2卷 153-171页

作者： Lilian Ellen Pino Joni E.Lima Mateus H.Vicente ariadne F.L.de Sá Francisco Pérez-Alfocea Alfonso Albacete Juliana L.Costa TomášWerner Thomas Schmülling Luciano Freschi Antonio Figueira Agustin Zsögön Lázaro E.P.Peres Laboratory of Plant Breeding Centro de Energia Nuclear na AgriculturaUniversity of Sao PauloSão PauloBrazil Laboratory of Hormonal Control of Plant Development Department of Biological SciencesEscola Superior de Agricultura‘Luiz de Queiroz’University of Sao PauloPiracicabaBrazil Botany Department ICBFederal University of Minas GeraisBelo HorizonteBrazil Department of Plant Nutrition CEBAS-CSICCampus Univ.EspinardoMurciaSpain Institute of Biology/Applied Genetics Freie Universität BerlinBerlinGermany Institute of Biology University of GrazSchubertstraße 518010 GrazAustria Biosciences Institute University of Sao PauloSão PauloBrazil Plant Sciences Department Federal University of ViçosaViçosaBrazil

Tomato production is influenced by shoot branching,which is controlled by different *** we produced tomato plants overexpressing the cytokinin-deactivating gene CYTOKININ OXYDASE 2(CKX2).CKX2-overexpressing(CKX2-OE)plants showed an excessive growth of axillary shoots,the opposite phenotype expected for plants with reduced cytokinin content,as evidenced by LC-MS analysis and ARR5-GUS *** TCP transcription factor SlBRC1b was downregulated in the axillary buds of CKX2-OE and its excessive branching was dependent on a functional version of the GRAS-family gene LATERAL SUPPRESSOR(LS).Grafting experiments indicated that increased branching in CKX2-OE plants is unlikely to be mediated by root-derived *** CKX2-OE plants with transgenic antisense plants for the strigolactone biosynthesis gene CAROTENOID CLEAVAGE DIOXYGENASE(CCD7-AS)produced an additive phenotype,indicating independent effects of cytokinin and strigolactones on increased *** the other hand,CKX2-OE plants showed reduced polar auxin transport and their bud outgrowth was reduced when combined with auxin ***,CKX2-OE basal buds did not respond to auxin applied in the decapitated *** results suggest that tomato shoot branching depends on a fine-tuning of different hormonal balances and that perturbations in the auxin status could compensate for the reduced cytokinin levels in CKX2-OE plants.

关键词： CKX2 Hormones Plant development Solanum

Increase in Straight and Coiled Cylindrospermopsis raciborskii (Cyanobacteria) Populations under Conditions of Thermal De-Stratification in a Shallow Tropical Reservoir

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Journal of Water Resource and Protection 2011年第4期3卷 245-252页

作者： Maria do Carmo Bittencourt-Oliveira ariadne do Nascimento Moura Talita Caroline Hereman Enio Wocyli Dantas 不详

In recent decades, there have been frequent occurrences of the cyanobacterium Cylindrospermopsis raciborskii in northeastern Brazil. Little is known regarding the response of straight and coiled morphotypes to environmental conditions such as light intensity and water temperature. Samples were collected at the Mundaú reservoir (PE, Brazil) at six sampling depths in the dry and rainy season. Both morphotypes exhibited seasonal and vertical differences in densities. The reservoir was stratified in the dry season, with a predominance of the straight morphotype. The coiled morphotype exhibited greater densities in the lower strata and prove to be more susceptible to light. There was evident thermal de-stratification in the rainy season, with a predominance of the coiled morphotype in the surface layers. Thermal de-stratification favors an increase in both morphotypes by providing adequate conditions for growth, such as low light intensity and milder temperatures, which are characteristic of the winter season in the northeastern Brazil.

关键词： Cyanophyta Light Morphotype Northeastern Brazil Mundaú Reservoir

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不依赖教科书的语言学习

疯狂英语（新策略） 2019年第5期 32-34页

作者： ariadne 王普(编) 不详

常规的语言学习除了离不开语境以外,教科书也是很重要的核心学习材料。这篇文章,作者讲述的是自己不依赖教科书而进行的语言学习。作者分享了哪些与众不同的学习方式呢?一起来寻找答案吧!

关键词：语言学习教科书学习材料学习方式作者语境

Cylindrospermopsin in Water Supply Reservoirs in Brazil Determined by Immunochemical and Molecular Methods

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Journal of Water Resource and Protection 2011年第6期3卷 349-355页

作者： Maria do Carmo Bittencourt-Oliveira Viviane Piccin-Santos Paula Kujbida ariadne do Nascimento Moura 不详

It is reported for the first time in Brazil and South America the presence of cylindrospermopsin (CYN) in water supply reservoirs. CYN is a powerful hepatotoxic alkaloid implicated in outbreaks of human sicknesses. We detected CYN in different sources of water in Northeastern Brazil using molecular and immunological techniques. The highest concentrations of toxin occurred in the Jucazinho reservoir with the phytoplankton containing the potentially CYN-producing C. raciborskii and Sphaerospermopsis aphanizomenoides (previously known as Aphanizomenon aphanizomenoides). The polyketide synthase (PKS) and peptide synthetase (PS), which are directly related to the ability to produce CYN, were found in all the analyzed samples. The result of the present study emphasizes the need to improve monitoring of CYN in water bodies used for drinking and recreation, in order to avoid exposure of human populations to this toxin.

关键词： Aphanizomenon Cyanobacteria Cylindrospermopsis Raciborskii CYN Sphaerospermopsis Aphanizomenoides Toxin

Petrology,Geochemistry and Economic Aspects of the Alkaline Dyke in Anta Gorda Region,Doutor Ulysses,Parana,Brazil

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Open Journal of Geology 2019年第7期9卷 378-394页

作者： Jose Augusto Simoes Neto Leonardo Fadel Cury Marcell Leonard Besser ariadne Borgo Salla Talvikki Eeva Departamento de Geologia Federal University of ParanáCuritibaBrazil Geologic Survey of Brazil(CPRM) CuritibaBrazil

The rock types of the alkaline dyke from Anta Gorda region, in the township of Doutor Ulysses (State of Paraná), encompass three lithological groups that have been individualized for their distinct textural, structural, mineralogical and/or geochemical characteristics: phonolites s.s., amygdaloidal phonolites and trachyphonolites. These are rocks with magmatic derivation, in which the most important differentiation processes interpreted were immiscibility and fractional crystallization from the ultrabasic mantellic source with primary magma. Fenite epidotes were also classified in the study area, probably product of fenitization of the granitic wall rock. Due to the geographical proximity, some similarities between the phonolites and the rocks from the Barra do Teixeira alkaline plug or the Banhadão Alkaline Complex were expected. However, the results of the petrographic and geochemical analysis point to major resemblance between the phonolites and the lithotypes of the Mato Preto Alkaline Complex. The studied rocks contain concentrations of economic interest of important chemicals elements, mainly Rare Earth Elements—REE, trace elements and mineral substances, such as zeolites and fluorites.

关键词： Phonolites Rare Earth Elements Epidote Mineralization