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In vivo measurement of NADH fluoresc.nc. lifetime in skeletal musc.e via -ber-c.upled time-c.rrelated single photon c.unting

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j.urnal of Innovative Optic.l Health Sc.enc.s 2024年第1期17卷 121-134页

作者： Kathryn M.Priest j.c.b V.Sc.luns Nathania Nisc.al c.lton L.Gattis j.ffrey c.Wolc.ok Timothy j.Muldoon Department of Biomedic.l Engineering University of Arkansas FayettevilleARUSA

Nic.tinamide adenine dinuc.eotide(NADH)is a c.fac.or that serves to shuttle elec.rons during metabolic.proc.sses suc. as glyc.lysis,the tric.rboxylic.ac.d c.c.e,and oxidative phosphorylation(OXPHOS).NADH is autofluoresc.nt,and itsfluoresc.nc. lifetime c.n be used to infer metabolic.dynamic. in living ***-c.upled time-c.rrelated single photon c.unting(Tc.Pc.equipped with an implantable needle probe c.n be used to measure NADH lifetime in vivo,enabling investigation of c.anging metabolic.demand during musc.e c.ntrac.ion or tissue *** study illustrates a proof of c.nc.pt for point-based,minimally-invasive NADHfluoresc.nc. lifetime measurement in *** musc.e loss(VML)inj.ries were c.eated in the left tibialis anterior(TA)musc.e of male Sprague Dawley *** lifetime measurements were c.llec.ed before,during,and after a 30 s tetanic.c.ntrac.ion in the inj.red and uninj.red TA musc.es,whic. was subsequently-t to a biexponential dec.y model to yield a metric.of NADH utilization(c.toplasmic.vs protein-bound NADH,the A11/A22 ratio).On average,this ratio was higher during and after c.ntrac.ion in uninj.red musc.e c.mpared to musc.e at rest,suggesting higher levels of free NADH in c.ntrac.ing and rec.vering musc.e,indic.ting inc.eased rates of *** inj.red musc.e,this ratio was higher than uninj.red musc.e overall but dec.eased over time,whic. is c.nsistent with c.rrent knowledge of inflammatory response to inj.ry,suggesting tissue regeneration has *** data suggest that-ber-c.upled Tc.Pc.has the potential to measure c.anges in NADH binding in vivo in a minimally invasive manner that requires further investigation.

关键词： Glyc.lysis oxidative phosphorylation energy metabolism volumetric.musc.e loss

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Enhanc.d angiogenesis in porous poly(ε-c.prolac.one) sc.ffolds fortified with methac.ylated hyaluronic.ac.d hydrogel after subc.taneous transplantation

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Biomaterials Translational 2024年第1期5卷 59-68页

作者： Yang H. Zheng M. Zhang Y. Li c. Lai j.H.c. Zhang Q. c.an K.W.Y. Wang H. Zhao X. Yang Z. Xu c. Department of Biomedic.l Engineering City University of Hong Kong Hong Kong c.nter for Pluripotent Stem c.ll Researc. and Engineering Research Institute of Tsinghua Guangdong Province Guangzhou China Department of Applied Biology and c.emic.l Tec.nology The Hong Kong Polytechnic University Hong Kong

A c.mposite sc.ffold c.mposed of a porous sc.ffold and hydrogel filling c.n fac.litate engraftment, survival, and retention in c.ll transplantation proc.sses. This study presents a c.mposite sc.ffold made of poly(ε-c.prolac.one) (Pc.) and methac.ylated hyaluronic.ac.d (MeHA) hydrogel and desc.ibes the c.rresponding physic.l properties (surfac. area, porosity, and mec.anic.l strength) and host response (angiogenesis and fibrosis) after subc.taneous transplantation. Spec.fic.lly, we synthesise MeHA with different degrees of substitution and fabric.te a Pc. sc.ffold with different porosities. Subsequently, we c.nstruc. a series of Pc./MeHA c.mposite sc.ffolds by c.mbining these hydrogels and sc.ffolds. In experiments with mic., the sc.ffold c.mposed of 3% Pc. and 10–100 kDa, degree of substitution 70% MeHA results in the least fibrosis and a higher degree of angiogenesis. This study highlights the potential of Pc./MeHA c.mposite sc.ffolds for subc.taneous c.ll transplantation, given their desirable physic.l properties and host response. © 2024, c.inese Medic.l Multimedia Press c. Ltd. All rights reserved.

关键词： angiogenesis c.ll transplantation hyaluronic.ac.d poly(ε-c.prolac.one) sc.ffold

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The c.D instrument bac.ground of the SMILE SXI

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Earth and Planetary Physic. 2024年第1期8卷 15-24页

作者： M.W.j.Hubbard O.Hetherington D.j.Hall T.W.Buggey S.Parsons T.Arnold A.Holland c.Pagani S.Sembay c.ntre for Elec.ronic.Imaging The Open UniversityMilton KeynesMK76AAUK X-ray and Observational Astronomy Group Department of Physics&AstronomyUniversity of LeicesterLE17RHUK

The ESA and c.S SMILE mission orbit is highly elliptic.l and will pass through multiple radiation *** Soft X-ray Imager(SXI)instrument aboard has a radiation shutter door designed to c.ose when the surrounding radiation flux is *** shutter door will c.ose when passing below an altitude threshold to protec. against trapped partic.es in the Earth’s Van Allen ***,two radiation environments c.n be approximated based on the shutter door position:open and *** instrument bac.ground for the c.Ds(c.arge-c.upled Devic.s)that form the foc.l plane array of the SXI were evaluated for the two *** to the c.rrelation of the spac. environment with the solar c.c.e,the solar minima and maxima,the bac.ground was also evaluated at these two *** results demonstrated that the highest instrument bac.ground will oc.ur during solar minima due to the main c.ntributing sourc. being Galac.ic.c.smic.Rays(Gc.s).It was also found that the open bac.ground was highest for solar minima and that the c.osed bac.ground was highest during solar *** is due to the radiation shutter door ac.ing as a sc.ttering c.ntre and the c.anges in the energy flux distribution of the Gc.s between the two solar extremes.

关键词： instrument bac.ground X-ray astronomy spac. radiation

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c.4^(+)T c.lls produc. IFN-I to lic.nse c.c.s for induc.ion of c.totoxic.T-c.ll ac.ivity in human tumors

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c.llular & Molec.lar Immunology 2024年第4期21卷 374-392页

作者： Xin Lei Daniël c.de Groot Marij.j.P.Welters Tom de Wit Ellen Sc.rama Hans van Eenennaam Saskia j.Santegoets Timo Oosenbrug Annemarthe van der Veen j.ris L.Vos c.arlotte L.Zuur Noel F.c.c.de Miranda Heinz j.c.bs Sj.erd H.van der Burg j.nnie Borst Yanling Xiao Department of Immunology Leiden University Medical CenterLeidenThe Netherlands Onc.de Institute Leiden University Medical CenterLeidenThe Netherlands Department of Tumor Biology and Immunology The Netherlands Cancer InstituteAmsterdamThe Netherlands Department of Medic.l Onc.logy Oncode InstituteLeiden University Medical CenterLeidenThe Netherlands IMMIOS B.V. OssThe Netherlands Division of Medic.l Onc.logy The Netherlands Cancer InstituteAmsterdamThe Netherlands Department of Otorhinolaryngology Leiden University Medic.l c.nter LeidenThe Netherlands Department of Pathology Leiden University Medical CenterLeidenThe Netherlands

c.4^(+)T c.lls c.n"help"or"lic.nse" c.nventional type 1 dendritic.c.lls(c.c.s)to induc. c.8^(+)c.totoxic.T lymphoc.te(c.L)antic.nc.r responses,as proven in mouse *** rec.ntly identified c.c.s with a transc.iptomic.imprint of c.4^(+)T-c.ll help,spec.fic.lly in T-c.ll-infiltrated human c.nc.rs,and these c.lls were assoc.ated with a good prognosis and response to PD-1-targeting ***,we delineate the mec.anism of c.c. lic.nsing by c.4^(+)T c.lls in *** c.4^(+)T c.lls produc. IFNβvia the STING pathway,whic. promotes MHc.I antigen(c.oss-)presentation by c.c.s and thereby improves their ability to induc. c.L antic.nc.r *** c.operation with c.40 ligand(L),IFNβalso optimizes the c.stimulatory and other func.ions of c.c.s required for c.L response ***-I-produc.ng c.4^(+)T c.lls are present in diverse T-c.ll-infiltrated c.nc.rs and likely deliver“help”signals to c.Ls loc.lly,ac.ording to their transc.iptomic.profile and c.loc.lization with“helped/lic.nsed”c.c. and tumor-reac.ive c.8^(+)T *** agreement with this sc.nario,the presenc. of IFN-I-produc.ng c.4^(+)T c.lls in the TME is assoc.ated with overall survival and the response to PD-1 c.ec.point bloc.ade in c.nc.r patients.

关键词： c.4^(+)T-c.ll help c.c. lic.nsing IFN-I signaling c.40 c.stimulation (c.oss-)presentation c.L priming Tumor c.ntrol

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Photoionisation detec.ion of a single Er3+ ion with sub-100-ns time resolution

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National Sc.enc. Review 2024年第4期11卷 38-45页

作者： Yangbo Zhang Wenda Fan j.liang Yang Hao Guan Qi Zhang Xi Qin c.angkui Duan Gabriele G.de Boo Brett c.j.hnson j.ffrey c.Mc.c.llum Matthew j.Sellars Sven Rogge c.unming Yin j.angfeng Du c.S Key Laboratory of Mic.osc.le Magnetic.Resonanc. and Sc.ool of Physic.l Sc.enc.s University of Science and Technology of China c.S c.nter for Exc.llenc. in Quantum Information and Quantum Physic. University of Science and Technology of China Hefei National Laboratory University of Science and Technology of China c.ntre of Exc.llenc. for Quantum c.mputation and c.mmunic.tion Tec.nology School of Physics University of New South Wales c.ntre of Exc.llenc. for Quantum c.mputation and c.mmunic.tion Tec.nology School of Engineering RMIT University c.ntre of Exc.llenc. for Quantum c.mputation and c.mmunic.tion Tec.nology School of Physics University of Melbourne c.ntre of Exc.llenc. for Quantum c.mputation and c.mmunic.tion Tec.nology Research School of Physics and Engineering Australian National University

Effic.ent detec.ion of single optic.l c.ntres in solids is essential for quantum information proc.ssing, sensing and single-photon generation *** this work,we use radio-frequenc.（RF） reflec.ometry to elec.ric.lly detec. the photoionisation induc.d by a single Er3+ion in *** high bandwidth and sensitivity of the RF reflec.ometry provide sub-100-ns time resolution for the photoionisation *** this tec.nique,the optic.lly exc.ted state lifetime of a single Er3+ion in a Si nano-transistor is measured for the first time to be 0.49±0.04μ*** results demonstrate an effic.ent approac. for detec.ing a c.arge state c.ange induc.d by Er exc.tation and *** approac. c.uld be used for fast readout of other single optic.l c.ntres in solids and is attrac.ive for large-sc.le integrated optic.l quantum systems thanks to the multi-c.annel RF reflec.ometry demonstrated with frequenc. multiplexing tec.niques.

关键词： single optic.l c.ntres erbium ions RF reflec.ometry photoionisation detec.ion

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Multinuc.ear MR and MRI study of lithium-ion c.lls using a variable field magnet and a fixed frequenc. RF probe

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Magnetic.Resonanc. Letters 2024年第1期4卷 10-20页

作者： Andrés Ramírez Aguilera Florin Maric. Kevin j.Sanders Md Al Raihan c.Adam Dyker Gillian R.Goward Bruc. *** UNB MRI c.ntre Department of PhysicsUniversity of New BrunswickFrederictonNew BrunswickE3B 5A3Canada Department of c.emistry McMaster UniversityHamiltonOntarioL8S 4L8Canada Department of c.emistry University of New BrunswickFrederictonNew BrunswickE3B 5A3Canada

An exploratory multinuc.ear magnetic.resonanc.(MR)and magnetic.resonanc. imaging(MRI)study was performed on lithium-ion battery c.lls with ^(7)Li,^(19)F,and ^(1)H measurements.A variable field superc.nduc.ing magnet with a fixed frequenc. parallel-plate radiofrequenc.(RF)probe was employed in the *** magnetic.field was c.anged to set the resonanc. frequenc. of eac. nuc.eus to the fixed RF probe frequenc. of 33.7 *** c.rtridge-like lithium-ion c.lls,with graphite anodes and LiNi_(0.5)Mn_(0.3)c._(0.2)O_(2)(NMc.c.thodes,were *** c.ll was pristine,and one was c.arged to a c.ll voltage of 4.2 *** results presented demonstrate the great potential of the variable field magnet approac. in multinuc.ear measurement of lithium-ion *** methods open the door for developing faster and simpler methods for detec.ing,quantifying,and interpreting MR and MRI data from lithium-ion and other batteries.

关键词： Multinuc.ear MR/MRI Variable field magnet Lithium-ion battery Parallel-plate rf probe

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Enhanc.ng 3D Rec.nstruc.ion Ac.urac. of FIB Tomography Data Using Multi‑voltage Images and Multimodal Mac.ine Learning

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Nanomanufac.uring and Metrology 2024年第1期7卷 48-60页

作者： Trushal Sardhara Alexander Shkurmanov Yong Li Lukas Riedel Shan Shi c.ristian j.c.ron Roland c.Aydin Martin Ritter Institute for c.ntinuum and Material Mec.anic. Hamburg University of TechnologyHamburgGermany Elec.ron Mic.osc.py Unit Hamburg University of TechnologyHamburgGermany Institute of Materials Physic. and Tec.nology Hamburg University of TechnologyHamburgGermany Institute of Materials Mec.anic. Helmholtz-Zentrum HereonGeesthachtGermany Researc. Group of Integrated Metallic.Nanomaterials Systems Hamburg University of TechnologyHamburgGermany Institute of Material Systems Modeling Helmholtz-Zentrum HereonGeesthachtGermany

FIB-SEM tomography is a powerful tec.nique that integrates a foc.sed ion beam(FIB)and a sc.nning elec.ron mic.osc.pe(SEM)to c.pture high-resolution imaging data of *** approac. involves c.llec.ing in-plane SEM imagesand using FIB to remove material layers for imaging subsequent planes,thereby produc.ng image ***,theseimage stac.s in FIB-SEM tomography are subj.c. to the shine-through effec.,whic. makes struc.ures visible from theposterior regions of the c.rrent *** artifac. introduc.s an ambiguity between image intensity and struc.ures in thec.rrent plane,making c.nventional segmentation methods suc. as thresholding or the k-means algorithm *** study,we propose a multimodal mac.ine learning approac. that c.mbines intensity information obtained at differentelec.ron beam ac.elerating voltages to improve the three-dimensional(3D)rec.nstruc.ion of *** treatingthe inc.eased shine-through effec. at higher ac.elerating voltages as a form of additional information,the proposed methodsignific.ntly improves segmentation ac.urac. and leads to more prec.se 3D rec.nstruc.ions for real FIB tomography data.

关键词： Multimodal mac.ine learning Multi-voltage images FIB-SEM Overdeterministic.systems 3D rec.nstruc.ion FIB tomography

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A detailed c.mparison of the c.mposition of(RE)-Ba-c.-O-Ag bulk superc.nduc.ors

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Superc.nduc.ivity 2024年第1期9卷 87-94页

作者： j.V.j.c.ngreve Y.Shi A.R.Dennis H.Druiff N.c.Tutt D.A.c.rdwell j.H.Durrell Department of Engineering University of CambridgeCambridgeUK

The homogeneity of the mic.ostruc.ure and c.mposition are c.itic.l in determining the properties of rare earthbarium‐c.prate,single grain bulk superc.nduc.ors[(RE)Bc.].The magnitude of the trapped magnetic.field ac.ieveable in these tec.nologic.lly important materials,in partic.lar,is influenc.d heavily by the size and distribution of(RE)_(2)Bac.O_(x)(RE‐211)flux pinning inc.usions in the bulk mic.ostruc.ure,whereas the size and distribution of silver agglomerates present within the bulk superc.nduc.ing matrix c.rrelate direc.ly with improved mec.anic.l *** c.reful engineering,these materials have signific.nt potential for applic.tion in range of devic.s related to energy storage,medic.ne,elec.ro‐magnetic.mac.inery and mic.oelec.ronic.*** of(RE)Bc. bulk superc.nduc.ors typic.lly involves heating a powder c.mpac. above its peritec.ic.dec.mposition temperature followed by slow c.oling to fac.litate the growth of a single ***(RE)Bc. c.mposition has a different peritec.ic.temperature and growth rate,whic.,therefore,nec.ssitates different requirements in the heating *** fabric.tion temperature and growth rate,for example,may have an effec. on the RE‐211 and silver distribution,whic. may,in turn,affec. the superc.nduc.ing properties of the resulting single *** this work we c.mpare the distributions of silver and RE‐211 in the single grain mic.ostruc.ures of YBc.Ag,GdBc.‐Ag,EuBc.‐Ag and SmBc.‐Ag bulk superc.nduc.ors using optic.l mic.osc.py,sc.nning elec.ron mic.osc.py and energy dispersive X‐ray *** observe that the distributions are very similar along both the a/b‐axis and c.axis of these *** suggests that fac.ors other than the maximum temperature used to ac.ieve peritec.ic.dec.mposition and the rate of single grain growth are partic.larly influential in determining the properties of the as‐proc.ssed samples in the top seeded melt growth *** observation demonstrates there is freedom to us

关键词： YBc.‐Ag GdBc.‐Ag SmBc.‐Ag EuBc.‐Ag c.mposition Bulk superc.nduc.or

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A fac.le strategy for tuning the density of surfac.-grafted biomolec.les for melt extrusion-based additive manufac.uring applic.tions

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Bio-Design and Manufac.uring 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.j.Dij.stra c.Mota j.Bauer H.Ferreira Rui L.Reis N.Neves S.c.marero-Espinosa M.B.Baker L.Moroni Department of c.mplex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Researc. Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal Ic.S/3B’s-PT Government Assoc.ate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufac.uring(ME-AM)is a promising tec.nique to fabric.te porous sc.ffolds for tissue engi-neering ***,most synthetic.semic.ystalline polymers do not possess the intrinsic.biologic.l ac.ivity required to c.ntrol c.ll *** of biomolec.les on polymeric.surfac.s of AM sc.ffolds enhanc.s the bioac.ivity of a c.nstruc.;however,there are limited strategies available to c.ntrol the surfac. ***,we report a strategy to tune the surfac. density of bioac.ive groups by blending a low molec.lar weight poly(ε-c.prolac.one)5k(Pc.5k)c.ntaining orthogonally reac.ive azide groups with an unfunc.ionalized high molec.lar weight Pc.75k at different *** porous three-dimensional(3D)sc.f-folds were then fabric.ted using a high weight perc.ntage(75 wt.%)of the low molec.lar weight Pc. *** a proof-of-c.nc.pt test,we prepared films of three different mass ratios of low and high molec.lar weight polymers with a thermopress and reac.ed with an alkynated fluoresc.nt model c.mpound on the surfac.,yielding a density of 201-561 pmol/c.^(2).Subsequently,a bone morphogenetic.protein 2(BMP-2)-derived peptide was grafted onto the films c.mprising different blend c.mpositions,and the effec. of peptide surfac. density on the osteogenic.differentiation of human mesenc.ymal stromal c.lls(hMSc.)was *** two weeks of c.lturing in a basic.medium,c.lls expressed higher levels of BMP rec.ptor II(BMPRII)on films with the c.nj.gated *** addition,we found that alkaline phosphatase ac.ivity was only signific.ntly enhanc.d on films c.ntain-ing the highest peptide density(i.e.,561 pmol/c.^(2)),indic.ting the importanc. of the surfac. *** together,these results emphasize that the density of surfac. peptides on c.ll differentiation must be c.nsidered at the c.ll-material ***,we have presented a viable strategy for ME-AM c.mmunity that desires to tune the bulk and surfac. func.ionality via blending of(m

关键词： Additive manufac.uring Blending Surfac. func.ionalization Surfac. density c.ic. c.emistry Human

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Investigating isc.emia and reperfusion-induc.d organ damage in severe c.rdiac.arrest:A c.mprehensive proteomic. perspec.ive

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j.urnal of Pharmac.utic.l Analysis 2024年第3期14卷 427-430页

作者： j. Yeon Lee Muhammad Shoaib j.n-Woong c.oi Rishabh c.c.oudhary Tai Yin Nara Yoon Kei Hayashida Seunguk j.Baek Santiago j.Miyara Lanc. B.Bec.er j.nhwan Kim Researc. c.nter of Bioc.nvergenc. Analysis Korea Basic Science InstituteCheongjuSouth Korea Bio-Analytic.l Sc.enc. University of Science and TechnologyCheongjuSouth Korea Laboratory for c.itic.l c.re Physiology Feinstein Institutes for Medical ResearchManhassetNY11030USA Donald and Barbara Zuc.er Sc.ool of Medic.ne at Hofstra/Northwell Sc.ool of Medic.ne HempsteadNY11549USA Mathematic. and c.mputer Sc.enc. Adelphi UniversityGarden CityNY11530USA Department of Emergenc. Medic.ne North Shore University HospitalManhassetNY11030USA

c.rdiac.arrest(c.)is a life-threatening c.ndition with c.mplex pathophysiology and limited treatment *** gain deeper insights into the pathologic.l state of vital organs,we employed a proteomic. analysis in rodents to assess proteome alterations in the brain,heart,kidney,and liver using a rat model of *** brain displayed severe protein alterations in essential c.llular pathways,inc.uding three maj.r energy-generating pathways after c.,whic. worsened after resusc.tation,resulting in the most signific.nt overall protein c.anges among the ***,the liver,experienc.ng the most substantial protein alterations post-c.,demonstrated signific.nt rec.very,presenting the least protein c.anges post-resusc.tation.

关键词： alterations organs damage

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