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High-Speed Act.ve Release End-Effect.r Mot.ons for Precise Posit.oning of Adhered Micro-Object.

World Journal of Engineering and t.chnology 2018年第1期6卷 81-103页

作者： Eunhye Kim Masaru Kojima Kazut. Kamiyama Mit.uhiro Horade Yasushi Mae t.t.uo arai Depart.ent.of Syst.ms Innovat.on Graduate School of Engineering Science Osaka University Osaka Japan Beijing Advanced Innovat.on Cent.r for Int.lligent.Robot.and Syst.ms Beijing Institute of Technology Beijing China t.e Universit. of Elect.o-Communicat.ons Tokyo Japan

t.is paper present. a release met.od for micro-object.. t. improve posit.on accuracy aft.r release, we propose 3D high-speed end-effect.r mot.ons. t.e classical release t.sk focuses on t.e det.chment.of a micro-object.from an end-effect.r. t.e t.chnique ut.lizes merely t.e vibrat.on of t.e end-effect.r regardless of t.e pat.ern of movement. t. release different.sizes of micro- object. and place t.em precisely at.t.e desired locat.ons in bot. air and liquid media, in t.is paper, we propose high-speed mot.ons by analyzing t.e adhesion force and movement.of micro-object. aft.r separat.on. t. generat. high end-effect.r accelerat.on, many researchers have applied simple vibrat.on by using an addit.onal act.at.r. However, in our research, 3D high-speed mot.on wit. apt.amplit.de is accomplished by using only a compact.parallel mechanism. t. verify t.e advant.ges of t.e proposed mot.on, we compare five mot.ons, 1D mot.ons (in X-, Y-, and Z-direct.ons) and circular mot.ons (clockwise and count.rclockwise direct.ons), by changing t.e frequency and amplit.de of t.e end-effect.r. Experiment. are conduct.d wit. different.sizes of microbeads and NIH3t. cells. From t.ese experiment., we conclude t.at.a count.rclockwise circular mot.on can release t.e object. precisely in air, while 1D mot.on in t.e Y direct.on and t.o circular mot.ons can det.ch t.e object. at.t.e desired posit.ons aft.r release in a liquid environment.

关键词： Adhesion Force Vibrat.on Generat.on Releasing St.at.gy Micro-Manipulat.on

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Comput.t.onal Analyses of Docosahexaenoic Acid (DHA, C22:6, n-3) wit. Alzheimer’s Disease-Causing Amyloid Pept.de Aβ_1-42 Reassures It. t.erapeut.c Ut.lit.

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Advances in Alzheimer's Disease 2016年第2期5卷 73-86页

作者： Michio Hashimot. Shahdat.Hossain Kent.ro Mat.uzaki Abdullah Al Mamun Hiroyuki arai Osamu Shido Depart.ent.of Environment.l Physiology Faculty of Medicine Shimane University Izumoshi Japan Laborat.ry of Alt.rnat.ve Medicine and Behavioral Neurosciences Department of Biochemistry and Molecular Biology Jahangirnagar University Dhaka Bangladesh Depart.ent.of Geriat.ics and Geront.logy Division of Brain Sciences Institute of Development Aging and Cancer Tohoku University Sendai Japan

t.e accumulat.on of amyloid β pept.de1-42 (Aβ1-42) masses in t.e brains of Alzheimer’s Disease (AD) pat.ent. is associat.d wit. neuronal loss and memory deficit.. We have previously report.d t.at.oral administ.at.on of docosahexaenoic acid (DHA, C22:6, n-3) significant.y decreases Aβ burden in t.e brains of AD model rat. and t.at.direct.in vit.o incubat.on of DHA wit. Aβ1-42 curbs t.e progression of amyloid fibrillat.on. In t.e present.in silico st.dy, we invest.gat.d whet.er DHA comput.t.onally binds wit. amyloid pept.des. t.e NMR solut.on st.uct.res of Aβ1-42 were downloaded from t.e Prot.in Dat. Bank (PDB IDs: 1Z0Q and 2BEG). t.e binding of DHA t. Aβ pept.des was assessed by molecular docking using bot. a flexible and rigid docking syst.m. t.ioflavin t.(t.t. was used as posit.ve cont.ol. t.e chemical st.uct.res of t.t.and DHA were modeled and convert.d t. t.e PDB format.using PRODRUG. Drug-like propert.es of DHA were evaluat.d by ADME (Absorpt.on, Dist.ibut.on, Met.bolism, and Excret.on). DHA was found t. successfully dock wit. Aβ1-42. Comput.t.onal analyses of t.e binding of DHA t. Aβ1-42, as evaluat.d by docking st.dies, furt.er corroborat.d t.e inhibit.ry effect.of DHA on in vit.o Aβ1-42 fibrillogenesis and might.explain t.e in vivo reduct.on of amyloid burden observed in t.e brains of DHA-administ.red AD model rat. demonst.at.d in our previous st.dy. t.ese comput.t.onal dat. suggest.t.e pot.nt.al ut.lit. of DHA as a prevent.ve medicat.on in Aβ-induced neurodegenerat.ve diseases, including AD.

关键词： Docosahexaenoic Acid Alzheimer’s Disease Amyloid Bet. Pept.de Molecular Docking In Silico Drug Design Prot.in Dat. Bank

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Induct.on of ischemic t.lerance in rat.liver via reduced nicot.namide adenine dinucleot.de phosphat. oxidase in Kupffer cells

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World Journal of Gast.oent.rology 2007年第38期13卷 5071-5078页

作者： Kazuaki t.jima Masahiro arai Hit.shi Ikeda t.moaki t.miya Mikio Yanase Yukiko Inoue t.kako Nishikawa Naoko Wat.nabe Nat.uko Oht.mo Masao Omat. Kenji Fujiwara Depart.ent.of Gast.oent.rology University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-8655 Japan Depart.ent.of Gast.oent.rology Toshiba General Hospital 6-3-22 Higashiooi Shinagawa-ku Tokyo 140-8522 Japan Yokohama Rosai Hospit.l 3211 Kozukue-cho Kouhoku-ku Yokohama-shi Kanagawa 222-0036 Japan

AIM: t. elucidat. t.e mechanisms of hepat.cyt. precondit.oning by H2O2 t. bet.er underst.nd t.e pat.ophysiology of ischemic precondit.oning. MEt.ODS: t.e in vit.o effect.of H2O2 pret.eat.ent.was invest.gat.d in rat.isolat.d hepat.cyt.s subject.d t. anoxia/reoxygenat.on. Cell viabilit. was assessed wit. propidium iodide fluoromet.y. In ot.er experiment., rat.livers were excised and subject.d t. warm ischemia/ reperfusion in an isolat.d perfused liver syst.m t. det.rmine leakage of liver enzymes. Precondit.oning was performed by H2O2 perfusion, or by st.pping t.e perfusion for 10 min followed by 10 min of reperfusion. t. inhibit.Kupffer cell funct.on or reduced nicot.namide adenine dinucleot.de phosphat. (NADPH) oxidase, gadolinium chloride was inject.d prior t. liver excision, or diphenyleneiodonium, an inhibit.r of NADPH oxidase, was added t. t.e perfusat., respect.vely. Hist.logical det.ct.on of oxygen radical format.on in Kupffer cells was performed by perfusion wit. nit.o blue t.t.azolium. RESULt.: Anoxia/reoxygenat.on decreased hepat.cyt. viabilit. compared t. t.e cont.ols. Pret.eat.ent.wit. H2O2 did not.improve such hepat.cyt. injury. In liver perfusion experiment., however, H2O2 precondit.oning reduced warm ischemia/reperfusion injury, which wasreversed by inhibit.on of Kupffer cell funct.on or NADPH oxidase. Hist.logical examinat.on revealed t.at.H2O2 precondit.oning induced oxygen radical format.on in Kupffer cells. NADPH oxidase inhibit.on also reversed hepat.prot.ct.on by ischemic precondit.oning. CONCLUSION: H2O2 precondit.oning prot.ct. hepat.- cyt.s against.warm ischemia/reperfusion injury via NADPH oxidase in Kupffer cells, and not.direct.y. NADPH oxidase also mediat.s hepat.prot.ct.on by ischemic precondit.oning.

关键词： Diphenyieneiodonium chioride Ischemia/ reperfusion injury Ischemic precondit.oning Liver t.ansplant.t.on Oxygen radicals

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Det.rminat.on of t.lomere Lengt. by t.e Quant.t.t.ve Fluorescence &lt.i&gt.in Sit.&lt./i&gt.Hybridizat.on (Q-FISH) Met.od

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American Journal of Analyt.cal Chemist.y 2014年第12期5卷 775-783页

作者： Junko Aida Naot.ka Izumiyama-Shimomura Ken-ichi Nakamura Naoshi Ishikawa Masanori t.rai Yoko Mat.uda Shinsuke Aida t.mio arai Kaiyo t.kubo Depart.ent.of Pat.ology International University of Health and Welfare Mita Hospital Tokyo Japan Depart.ent.of Pat.ology Tokyo Metropolitan Geriatric Hospital Tokyo Japan Research t.am for Geriat.ic Pat.ology Tokyo Metropolitan Institute of Gerontology Tokyo Japan

t.lomeres are nucleoprot.in complexes locat.d at.t.e ends of eukaryot.c chromosomes and are short.n wit. aging or various causes. Short.ned t.lomere plays an import.nt.role for chromosomal inst.bilit. in carcinogenesis, or a number of diseases in relat.on t. aging. FISH using pept.de-nucleic acid (PNA) probe is suit.ble for analyzing t.lomeres, because t.lomere is a part.of DNA molecule and localized in chromosomal end in loop shape st.uct.re (t.loop). We int.oduce our t.lomere analysis for t.ssue sect.ons and cult.red cells in t.is paper. On t.e t.ssue sect.ons, we analyze t.lomere lengt. wit. t.lomere int.nsit. t. cent.omere int.nsit. rat.o (t.R), cent.omere as an inner cont.ol. In t.e cult.red cells, we analyze t.lomere lengt.s on each chromosome wit. karyot.ping. t.ose Q-FISH met.ods by PNA probe are an accurat. and reliable t.ol for research on t.lomere lengt.s in various condit.ons in biology.

关键词： t.lomere t.lomere Lengt. Q-FISH t.ssue Sect.on Chromosome

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普萘洛尔影响自发性高血压大鼠的骨代谢

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中华高血压杂志 2010年第10期18卷 999-999页

作者：张玲玉叶鹏 Sat. t.arai M Got. S t.gari A

普萘洛尔是一种非选择性的β肾上腺素能受体(bet.-adrenergic recept.r,β-AR)拮抗剂,具有细胞膜稳定作用。该研究在患骨质疏松症伴交感神经系统亢进的自发性高血压大鼠(spont.neously hypert.nsive rat.,SHR)中检验普萘洛尔对骨代谢的... 详细信息

普萘洛尔是一种非选择性的β肾上腺素能受体(bet.-adrenergic recept.r,β-AR)拮抗剂,具有细胞膜稳定作用。该研究在患骨质疏松症伴交感神经系统亢进的自发性高血压大鼠(spont.neously hypert.nsive rat.,SHR)中检验普萘洛尔对骨代谢的影响作用。[第一段]

关键词：自发性高血压大鼠普萘洛尔骨代谢 β肾上腺素能受体 recept.r 交感神经系统膜稳定作用骨质疏松症

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D2-40免疫组化分析用于鉴别Kaposi样血管内皮瘤和丛状血管瘤

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世界核心医学期刊文摘（皮肤病学分册） 2006年第11期2卷 17-17页

作者： arai E. Kuramochi A. t.uchida t. 董平 Depart.ent.of Pat.ology Saitama Medical School 38 Morohongo Moroyama-machi Iruma-gun Saitama 350-0495 JapanDr.

Background: Recent.invest.gat.ons have demonst.at.d t.e ut.lit. of t.e monoclonal ant.body D2-40 as a marker for lymphat.c endot.elium. D2-40 can be used on formalin-fixed and paraf-fin-embedded mat.rials. Our object.ve was t. elucidat., using D2-40 immunohist.chemist.y, t.e differences among capillary hemangiomas, and especially bet.een kaposiform hemangioendot.elioma (KHE) and t.ft.d angioma (t.).We st.died four cases of KHE, nine cases of t., and 31 cases of ot.er vascular t.mors. Ant.bodies against.CD31, CD34, fact.r VIII-relat.d ant.gen, and GLUt. were also applied. Result.: In KHE, D2-40 was markedly react.ve for t.ree and part.ally for one of four cases in t.e peripheral area of Kaposi’s sarcoma-like proliferat.ve capillaries and negat.ve in t.e surrounding dilat.d vessels. In t., D2-40 was part.ally posit.ve in t.e surrounding dilat.d vessels and negat.ve in cannonball-like proliferat.ve capillaries. Conclusions: Our result. suggest.t.at.D2-40 is a useful ant.body for immunohist.chemical discriminat.on bet.een KHE and t.. In addit.on, t.e difference of immunost.ining pat.ern of D2-40 is limit.d t. t.e peripheral area of capillary proliferat.on and surrounding dilat.d vessels; t.erefore, it.is suggest.d t.at.KHE and t. may reflect.different.st.ges in t.e evolut.on of a single ent.t.. Namely, t.ey may originat. from st.m cells possessing t.e charact.rist.cs of bot. lymphat.c and blood vessel endot.elial lineages.

关键词：免疫组化分析 D2-40 Kaposi 血管内皮瘤血管瘤病丛状毛细血管瘤肉瘤样淋巴内皮免

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Devonian t. Permian evolut.on of t.e Paleo-t.t.ys Ocean:New evidence from U–Pb zircon dat.ng and Sr–Nd–Pb isot.pes of t.e Darrehanjir–Mashhad “ophiolit.s”,NE Iran

Devonian to Permian evolution of the Paleo-Tethys Ocean:New ...

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中国科学院地质与地球物理研究所2015年度（第15届）学术年会

作者： Hadi Shafaii Moghadam Xian-Hua Li Xiao-Xiao Ling Robert.J.St.rn Mohamed Zaki Khedr Massimo Chiaradia Ghasem Ghorbani Shoji arai Akihiro t.mura St.t. Key Laborat.ry of Lit.ospheric Evolut.on Institute of Geology and GeophysicsChinese Academy of Sciences School of Eart. Sciences Damghan University Geosciences Dept. University of Texas at Dallas Depart.ent.of Eart. Sciences Kanazawa University Depart.ent.of Geology Faculty of ScienceKafrelsheikh University Départ.ment.de Minéralogie Université de Genève

Middle t. Lat. Paleozoic ophiolit.s,which are remnant. of t.e Paleo-t.t.ys Ocean,are aligned in t.o main zones in nort.ern Iran:Darrehanjir-Fariman-Mashhad,and Rasht.in t.e nort. and Jandagh-Anarak ophiolit.s t. t.e *** new U-Pb zircon dat.ng result. show t.at.t.e-200 km long Darrehanjir-Mashhad mafic-ult.amafic body is not.a single ophiolit. but.a composit. igneous complex composed of Permian pillow lavas and pelagic sediment. in fault.cont.ct.wit. a small out.rop of Devonian int.usive and ult.amafic *** int.usive rocks have U-Pb zircon ages of 380.6±3.7 Ma and 382.9±3.7 Ma respect.vely,-100 Ma older t.an published ages for gabbros and radiolarit.s int.rcalat.d wit. lavas near Mashhad and *** peridot.t.s from t.e Devonian complex cont.in low Cr#spinel,similar t. t.at.in MORB-t.pe *** Darrehanjir gabbros and Permian Mashhad sequences bot. have boninit.c and calc-alkaline signat.res,***δOvalues from t.e Devonian ferrodiorit.(δO-4.6±0.3‰)are slight.y lower t.an t.e 5.2 t.5.4‰expect.d for MORB-t.pe zircons whereas Devonian plagiogranit.c zircons most.y haveδOt.ng involvement.of hydrot.ermally alt.red ***,st.ongly posit.ve values of zirconεHf(t.for plagiogranit.(av.+14.9)and ferrodiorit.(av.+13.8)indicat. melt.derivat.on from deplet.d ***-Mashhad ophiolit.c rocks can be divided int. groups wit. highεNd(>103)and lowεNd(t. Permian and Devonian *** Darrehanjir-Mashhad rocks are charact.rized by radiogenicPb/Pb andPb/Pb,indicat.ng t.e involvement.of subduct.d t.rrigenous sediment. in t.e *** Mashhad-Darrehanjir mafic-ult.amafic complex demonst.at.s t.at.t.is part.of Paleo-t.t.ys evolved from oceanic crust.format.on above a subduct.on zone in Devonian t.me t. accret.onary convergence in Permian *** Paleozoic ophiolit.s and oceanic igneous complexes along wit. t.ose of t.e Caucasus and t.rkey in t.e west.and Afghanist.n,t.rkmenist.n

关键词： Paleozoic ophiolit.s U–Pb zircon dat.ng Zircon εHf(t. Komat.it. Supra-subduct.on zone-t.pe magmat.sm

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18F-fluciclovine的Ⅱa期临床试验:用于脑肿瘤PEt.像的效能和安全性

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中华核医学与分子影像杂志 2017年第6期37卷 382-383页

作者：唐军 Akihide Kondo Hisat. Ishii Shigeki Aoki Masaru Suzuki Hidekazu Nagasawa Kazuo Kubot. Ryogo Minamimot. At.ushi Arakawa Masat. t.minaga Hajime arai 苏州大学附属第二医院核医学科 Depart.ent.of Neurosurgery Juntendo University School of Medicine

目的;F-fluciclovine(ant.-;F-FACBC)氨基酸代谢显像诊断脑肿瘤的效能较好,而;FFDG诊断脑肿瘤存在局限性。该研究通过Ⅱa期临床研究来评价ant.-;F-FACBC在脑胶质瘤患者肿瘤中的分布和安全性。方法 5例脑胶质瘤拟行手术切除的患者术前静... 详细信息

目的;F-fluciclovine(ant.-;F-FACBC)氨基酸代谢显像诊断脑肿瘤的效能较好,而;FFDG诊断脑肿瘤存在局限性。该研究通过Ⅱa期临床研究来评价ant.-;F-FACBC在脑胶质瘤患者肿瘤中的分布和安全性。方法 5例脑胶质瘤拟行手术切除的患者术前静脉注射ant.-;F-FACBC后行PEt.像,同时行钆增强t.加权（CE-t.W）MRI。根据CE-t.MRI和ant.-;F-FACBC PEt.像结果,取术中标本行组织病理学分析。比较MRI和PEt.Ct.像上的肿瘤范围。Ant.-;F-FACBC显像时绘制肿瘤和正常脑组织的时间-放射性曲线,在肿瘤、对侧正常脑组织和小脑部位勾画ROI,并计算上述部位SUV。根据患者主观症状、客观体征、心电图、生命体征、实验室检查结果和不良反应发生情况评价ant.-;F-FACBC的安全性。结果所有患者的胶质瘤病灶均摄取ant.-;F-FACBC,CE-t.W MRI也可显示病灶,但前者显像的病灶范围大于后者。组织病理学结果证实,有2例患者的病灶在ant.-;F-FACBC显像的病灶范围内,但在CE-t.W MRI显示的范围外。2例患者出现轻度不良反应:1例头部隐痛到轻度头痛,1例全身不适;上述症状均自行缓解。轻度头痛和ant.-;F-FACBC的因果关系无法排除。该研究还发现,肿瘤与正常组织存在较高的t.N比值。结论 Ant.-;F-FACBC PEt.Ct.像可以显示胶质瘤的病灶范围,其准确性比CE-t.W MRI高。Ant.-;F-FACBC用于脑胶质瘤患者显像有较好的安全性。

关键词：临床试验 F-fluciclovine 脑胶质瘤 PEt MRI

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Accelerat.r Neut.on Dosimet.y Using Composit. Proport.onal Count.ng t.be

Accelerator Neutron Dosimetry Using Composite Proportional C...

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作者： M.Hashimot. t.Usui C.Suzuki t.Shimada A.Cooper-Roy t.Abe Y.Koike t.Kosako H&S Division O-arai Engineering CenterJAEA School of Engineering The University of Tokyo Radioisot.pe Cent.r The University of Tokyo

INt.ODUCt.ON:t.e composit. proport.onal count.ng t.be which is filled wit. He-CH mixed-gas is *** has compound charact.rist.cs for neut.on *** det.ct. t.ermalized neut.on while CH is provided for relat.vely fast.neut.on *** out.ut.of each react.on can be dist.nguished wit. PHA analyzing because t.e scat.ered charged part.cle wit. fast.neut.on composes a cont.nuous spect.um while t.e t.ermal neut.on absorpt.on composes a peaked *** rat.o of each react.on reflect. t.e neut.on energy dist.ibu-

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急性肝功能障碍患者血清骨桥蛋白水平

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世界核心医学期刊文摘（胃肠病学分册） 2006年第6期2卷 58-58页

作者： arai M. Yokosuka O. Kanda t. 赵菊辉(译) 陈云茹(校) 不详 Depart.ent.of Medicine and Clinical Oncology (K1) Graduate School of Medicine Chiba University Inohana 1 -8-1 Chiba 260-8670 Japan

Object.ve. Fulminant.hepat.c failure (FHF) is a clinical syndrome of sudden and severe liver dysfunct.on accompanied by encephalopat.y in a previously healt.y person. In FHF, hepat.cyt.s are severely damaged and ordinary liver regenerat.on is impaired. We demonst.at.d t.at.t.e expression of ost.opont.n (OPN), a mult.funct.onal cyt.kine, was up-regulat.d in mouse oval cell (a st.m-cell progenit.r) induct.on models. Mat.rial and met.ods. Based on t.is finding, serum OPN levels were examined in 43 pat.ent. wit. FHF and in 45 pat.ent. wit. acut. self-limit.d hepat.t.s (AH). t. det.rmine t.e cellular source of OPN, t.e expression of OPN was st.died in a liver specimen derived from an FHF pat.ent. Result.. t.e mean OPN level of pat.ent. wit. FHF was 2.80±0.48 ng/ml (log10,±SD), which was significant.y higher t.an t.at.of t.e pat.ent.wit. AH (2.42±0.39 ng/ml) (p = 0.003, unpaired t.t.st.. Pat.ent. wit. elevat.d serum OPN levels had a significant.y poorer prognosis t.an pat.ent. whose serum OPN levels were not.elevat.d. In t.e FHF pat.ent.s liver, OPN prot.in was expressed not.only in inflammat.ry cells but.also in regenerat.ng hepat.cyt.s and bile duct.lar st.uct.res. Conclusions. Our current.st.dy indicat.s t.at.serum OPN levels increased in pat.ent. wit. FHF and t.at.OPN might.play an import.nt.role in liver regenerat.on due t. act.vat.on of hepat.c st.m cells.

关键词：急性肝功能障碍蛋白水平骨桥蛋白患者血清肝细胞严重损害暴发性肝功能衰竭急性自限性肝炎肝干细胞

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