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Development of a multicellular 3D-bioprinted microtissue model of human periodontal ligament-alveolar bone biointerface:Towards a pre-clinical model of periodontal diseases and personalized periodontal tissue engineering

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Genes & Diseases 2022年第4期9卷 1008-1023页

作者： Murat Taner Vurat ŞükranŞeker Özge Lalegül-Ülker Mahmut Parmaksiz Ayşe Eser Elçin Yaşar Murat Elçin Tissue Engineering Biomaterials and Nanobiotechnology LaboratoryAnkara University Faculty of ScienceAnkara University Stem Cell InstituteAnkara 06100Turkey Biovalda Health Technologies Inc.Ankara 06100Turkey

While periodontal (PD) disease is among principal causes of tooth loss worldwide, regulation of concomitant soft and mineralized PD tissues, and PD pathogenesis have not been completely clarified yet. Besides, relevant pre-clinical models and in vitro platforms have limitations in simulating human physiology. Here, we have harnessed three-dimensional bioprinting (3DBP) technology for developing a multi-cellular microtissue model resembling PD ligament-alveolar bone (PDL-AB) biointerface for the first time. 3DBP parameters were optimized;the physical, chemical, rheological, mechanical, and thermal properties of the constructs were assessed. Constructs containing gelatin methacryloyl (Gel-MA) and hydroxyapatite-magnetic iron oxide nanoparticles showed higher level of compressive strength when compared with that of Gel-MA constructs. Bioprinted self-supporting microtissue was cultured under flow in a microfluidic platform for >10 days without significant loss of shape fidelity. Confocal microscopy analysis indicated that encapsulated cells were homogenously distributed inside the matrix and preserved their viability for >7 days under microfluidic conditions. Immunofluorescence analysis showed the cohesion of stromal cell surface marker-1+ human PDL fibroblasts containing PDL layer with the osteocalcin+ human osteoblasts containing mineralized layer in time, demonstrating some permeability of the printed constructs to cell migration. Preliminary tetracycline interaction study indicated the uptake of model drug by the cells inside the 3D-microtissue. Also, the non-toxic levels of tetracycline were determined for the encapsulated cells. Thus, the effects of tetracyclines on PDL-AB have clinical significance for treating PD diseases. This 3D-bioprinted multi-cellular periodontal/osteoblastic microtissue model has potential as an in vitro platform for studying processes of the human PDL.

关键词： 3D bioprinting Alveolar bone Microtissue model Organ-on-a-chip .Periodontal ligament Periodontal tissue engineering Periodontal-osteoblastic biointer face Periodontium-on-a-chip

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Taking into Account Density Fluctuations in a Solvent in a Model of Dissolution

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Open Journal of Physical Chemistry 2019年第4期9卷 204-215页

作者： I. A. ar’ev Kyiv Ukraine

Earlier it was shown by different authors that there are cavities (vacancies, holes) in any liquid. The cavities should play a prominent role in dissolution processes. Nevertheless this fact was ignored in previous model of dissolution. The sizes of the cavities in different solvents containing benzene molecules were determined using solvent induced spectral shift method. The measurements of S1←S0 benzene transition spectral shifts permit to conclude that 1) macroscopic excess volumes play an almost negligible role in processes of benzene dissolution in very different solvents and 2) the minimal size of the cavity in water able to accommodate benzene molecule coincides with the solute size. Generalization of this conclusion to other nonpolar aromatics leads to evaluation contraction of the solutes under aqueous solvent influence permits to predict the solubility values of other aromatics in water and to evaluate effect of enhancement hydrate cell around these molecules on solubility.

关键词： Solubility Solvent-Induced Spectral Shift Microscopic Balance of Volumes Fluctuation Cavities Aqueous Solvent Solute Contraction Hydrate Shell strengthening

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抗HLA－A2单抗重链可变区基因的克隆及其序列分析

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中国免疫学杂志 1995年第6期11卷 355-358页

作者：苏娜 ar.,EA 同济医科大学免疫学教研室

从５株抗ＨＬＡ－Ａ２单抗杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术，扩增和克隆出单抗可变区重链基因，并采用双脱氧链式终止法及计算机基因文库测定和分析了其全部的氨基酸序列，对抗体的生物学特性的研究具有重要意... 详细信息

从５株抗ＨＬＡ－Ａ２单抗杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术，扩增和克隆出单抗可变区重链基因，并采用双脱氧链式终止法及计算机基因文库测定和分析了其全部的氨基酸序列，对抗体的生物学特性的研究具有重要意义，为构建基因工程抗体奠定了基础。

关键词：单克隆抗体基因克隆序列分析脱氧核糖核酸

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Suppression of the External MitochondrialNADPH Dehydrogenase, NDB1, in arabidopsisthaliana Affects Central Metabolism andVegetative Growth

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Molecular Plant 2014年第2期7卷 356-368页

作者： Wallström S Florez-Sarasa I araújo WL Aidemark M Fernández-Fernández M Fernie ar Ribas-Carbó M Rasmusson AG Department of Biology Lund University Biology building Sölvegatan 35 SE-223 62 Lund Sweden

Ca2＋-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPHdehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript wassuppressed by 80% in arabidopsis thaliana plants, and external Ca2＋-dependent NADPH dehydrogenase activity becameundetectable in isolated mitochondria. This was linked to a decreased level of NADP＋ in rosettes of the transgenic ***-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of 14C-glucose was increased. On soil, NDBl-suppressing plants had a decreased vegetative biomass, but leaf maximumquantum efficiency of photosystem Ⅱ and CO2 assimilation rates, as well as total respiration, were similar to the wild-type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealeddecreased levels of sugars, citric acid cycle intermediates, and amino acids in the transgenic lines. The NDBl-suppressioninduced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. Thetranscriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thalianaoverexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP（H）reduction levels, which in turn affect central metabolism and growth, and interact with defense signaling.

关键词： citric acid cycle metabolic profiling mitochondrial respiration NADPH RNA interference type II NAD(P)Hdehydrogenase vegetative growth

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△_(h)-GOULD-HOPPER APPELL POLYNOMIALS

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Acta Mathematica Scientia 2021年第4期41卷 1196-1222页

作者： Mehmet Ali ÖZarSLAN Banu YILMAZ YASar Department of Mathematics Eastern Mediterranean UniversityFamagustaNorth Cyprus via Mersin 10Turkey

In this paper,we introduce the △_(h)-Gould-Hopper Appell polynomials An(x,y;h)via h-Gould-Hopper polynomials G_(n)^(h)(x,y).These polynomials reduces to △_(h)-Appell polynomials in the case y=0,△-Appell polynomials in the case y=0 and h=2D-Appell polynomials in the case h→0,2D △-Appell polynomials in the case h=1 and Appell polynomials in the case h→0 and y=*** obtain some well known main properties and an explicit form,determinant representation,recurrence relation,shift operators,difference equation,integro-difFerence equation and partial difference equation satisfied by *** satisfied by △_(h)-Gould-Hopper Appell polynomials reduce to determinant of all subclass of the usual ***,shift operators and difference equation satisfied by these polynomials reduce to recurrence,shift operators and difference equation of △_(h)-Appell polynomials,△-Appell polynomials;recurrence,shift operators,differential and integro-differential equation of 2D-Appell polynomials,recurrence,shift operators,integrodifference equation of 2D △-Appell polynomials,recurrence,shift operators,differential equation of Appell polynomials in the corresponding *** the special cases of the determining functions,we present the explicit forms,determinants,recurrences,difference equations satisfied by the degenerate Gould-Hopper Carlitz Bernoulli polynomials,degenerate Gould-Hopper Carlitz Euler polynomials,degenerate Gould-Hopper Genocchi polynomials,△_(h)-Gould-Hopper Boole polynomials and △_(h)-Gould-Hopper Bernoulli polynomials of the second *** particular cases of the degenerate Gould-Hopper Carlitz Bernoulli polynomials,degenerate Gould-Hopper Genocchi polynomials,△_(h)-Gould-Hopper Boole polynomials and △_(h)-Gould-Hopper Bernoulli polynomials of the second kind,corresponding determinants,recurrences,shift operators and difference equations reduce to all subclass of degenerate so-called families except for Genocchi polynomials recurrence,shift operators,an

关键词： △_(h)-Appell polynomials determinant recurrence difference equation

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新西兰道路管理

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公路交通科技 1996年第A期13卷 119-128页

作者： Kenn.,ar 陈国靖

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海浪（海口湾国家海洋公园-帆船帆板公共游艇码头建筑/灯塔）

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城市环境设计 2024年第1期147卷 98-103页

作者：浅野浩克洪韵王陈思田刘一青马凯蒂 Creat ar

海口湾国际海洋公园位于中国南部海南岛海口市。海洋体育中心定期在这个海洋公园举行国际游艇和冲浪比赛，中心内包括海洋文化展览、文化活动以及向公众开放的咖啡馆和酒吧。我们还设计了位于码头外围的灯塔，作为整体设施的一部分。考...

海口湾国际海洋公园位于中国南部海南岛海口市。海洋体育中心定期在这个海洋公园举行国际游艇和冲浪比赛，中心内包括海洋文化展览、文化活动以及向公众开放的咖啡馆和酒吧。我们还设计了位于码头外围的灯塔，作为整体设施的一部分。考虑到海洋和海洋文化背景，我们为海洋体育中心和灯塔都采用了“海浪”的概念，使码头周围的环境与多个设施通过同一种设计语言获得完整性。灯塔有一个垂直的波浪形白色百叶窗立面，沿着收缩的圆柱体结合了各种斜角三角形横截面，形成了一个融入海洋景观的立面。

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广西大瑶山社垌钨多金属矿床He-ar、H-O同位素特征及其地质意义

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中国钨业 2017年第2期32卷 1-7页

作者：方贵聪康志强冯佐海付伟蒋兴洲桂林理工大学地球科学学院广西隐伏金属矿产勘查重点实验室广西桂林541004 广西遥感中心广西南宁530023

社垌钨多金属矿床位于大瑶山隆起区东南部,是近年来发现并受到广泛关注的大型矿床,目前对其成矿流体来源,尤其是否存在壳幔相互作用机制尚缺乏深入认识。通过对矿区流体包裹体He-ar、H-O同位素的研究,结果显示,矿区斑岩型铜矿的~3He/~4H... 详细信息

社垌钨多金属矿床位于大瑶山隆起区东南部,是近年来发现并受到广泛关注的大型矿床,目前对其成矿流体来源,尤其是否存在壳幔相互作用机制尚缺乏深入认识。通过对矿区流体包裹体He-ar、H-O同位素的研究,结果显示,矿区斑岩型铜矿的~3He/~4He为0.7~1.46 Ra,^(40)ar/^(36)ar为294.02~316.96;石英脉-夕卡岩型钨钼矿~3He/~4He为0.46~0.67 Ra,^(40)ar/^(36)ar为303.83~388.62,成矿流体δD_(V-SMOW_介于-84‰^-61‰,δ^(18)O_(H_2O)为-0.48‰~5.09‰,表明石英脉-夕卡岩型钨钼矿和斑岩型铜矿成矿流体主要为岩浆水,成矿后期有较多大气饱和水的混入,成矿过程均有不同程度的幔源流体参与,前者的成矿流体幔源He含量为10.7%~15.5%,后者成矿流体幔源He含量达16.3%~33.9%。幔源流体加入是由矿床所处的大地构造背景所决定的,石英脉型钨钼成矿幔源流体的加入是由于扬子板块和华夏板块于加里东晚期发生碰撞导致地幔物质上涌而产生,斑岩铜矿地幔流体的混入则因为燕山晚期大陆边缘持续伸展、钦杭结合带较为薄弱而产生强烈壳幔相互作用而形成。

关键词： He-ar同位素 H-O同位素成矿流体大瑶山隆起社垌钨多金属矿床斑岩铜矿

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COMMUNICATION OF NUCLEar DATA PROGRESS No.11(1994)

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中国核科技报告 1994年第S1期 20-21页

作者： Chinese Nucle- ar Data Center

This is the eleventh issue of Communication of Nuclear Data Progress (CNDP), in which the nuclear data progress in China during the last year is carried, including the measurements ofZn (n, p),Ir (n, 2n)andEu (n,γ) activation cross sections, the level structure ofGd andW; photonuclear data calculations ofAl,Fe andBi, introduction to the codes SPEC and DDCS, the evaluations and calculations of reactionsFe (n, x)Cr,Mn,Au (n, 3n) and (n, 4n), an open problem on photon production calculation, intermediate-high energy nuclear reaction kinetic simulation and QMD, systematics of H (n, n); the evaluation of neutron induced data onFe, nuclear data sheets update for A=52, 54, 195, level spin assignment of superdeformed bands; Chinese Evaluated Nuclear Parameter Library (CENPL) (Ⅲ), an auxiliary plotting code of UNF code, management program system of Chinese evaluated nuclear decay database; a method for evaluation o

关键词： data Fe COMMUNICATION OF NUCLEar DATA PROGRESS No.11 QMD

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Plasmid profiling and antibiotics resisitance of Escherichia coli strains isolated from Mytilus galloprovincialis and seawater

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Journal of Coastal Life Medicine 2014年第9期 689-693页

作者： Cumhur Avşar İsmet Berber Department of Biology Faculty of Science and ArtsSinop UniversitySinop57000Turkey

Objective:To investigate plasmid DNA profiles and the antibiotic resistance of a total of 41 strains of Escherichia coli(***)isolated from seawater and mussel collected from 15 different sampling stations in Sinop,***:Most probable number technique was used for detection of *** susceptibilities of the isolates were determined by the disc diffusion *** DNA of the strains was extracted by the alkaline lyses ***:According to morphological and physiological properties,it was determined that the isolates belonged to *** *** susceptibility of the strains was determined against seven standard drugs using disc diffusion *** isolates were resistant to bacitracin(100%),novobiocin(100%),ampicillin(12.5%),tetracycline(7.5%),ceftazidime(5%)and imipenem(2.5%),respectively,whereas the strains were susceptible to polymyxin B(100%).The multiple antibiotic resistance values for the strains were found in range from 0.28 to *** addition,plasmid DNA analyses results confirmed that 22 strains harbored a single or more than two plasmids sized approximately between 24.500 to 1.618 *** high-size plasmid(14.700 bp)was observed as common in 21 of all ***:As a result,our study indicated that the presence of antibiotic resistant *** strains in seawater and mussel might be potential risk for public health issue.

关键词： Escherichia coli Antibiogram Plasmid Mytilus galloprovincialis Seawater

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