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Development.of a mult.cellular 3D-bioprint.d microt.ssue model of human periodont.l ligament.alveolar bone bioint.rface:t.wards a pre-clinical model of periodont.l diseases and personalized periodont.l t.ssue engineering

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Genes & Diseases 2022年第4期9卷 1008-1023页

作者： Murat.0}ner Vurat.ŞükranŞeker Özge Lalegül-Ülker Mahmut.Parmaksiz Ayşe Eser Elçin Yaşar Murat.Elçin t.ssue Engineering Biomaterials and Nanobiotechnology LaboratoryAnkara University Faculty of ScienceAnkara University Stem Cell InstituteAnkara 06100Turkey Biovalda Healt. t.chnologies Inc.Ankara 06100Turkey

While periodont.l (PD) disease is among principal causes of t.ot. loss worldwide, regulat.on of concomit.nt.soft.and mineralized PD t.ssues, and PD pat.ogenesis have not.been complet.ly clarified yet. Besides, relevant.pre-clinical models and in vit.o plat.orms have limit.t.ons in simulat.ng human physiology. Here, we have harnessed t.ree-dimensional bioprint.ng (3DBP) t.chnology for developing a mult.-cellular microt.ssue model resembling PD ligament.alveolar bone (PDL-AB) bioint.rface for t.e first.0}me. 3DBP paramet.rs were opt.mized;t.e physical, chemical, rheological, mechanical, and t.ermal propert.es of t.e const.uct. were assessed. Const.uct. cont.ining gelat.n met.acryloyl (Gel-MA) and hydroxyapat.t.-magnet.c iron oxide nanopart.cles showed higher level of compressive st.engt. when compared wit. t.at.of Gel-MA const.uct.. Bioprint.d self-support.ng microt.ssue was cult.red under flow in a microfluidic plat.orm for >10 days wit.out.significant.loss of shape fidelit.. Confocal microscopy analysis indicat.d t.at.encapsulat.d cells were homogenously dist.ibut.d inside t.e mat.ix and preserved t.eir viabilit. for >7 days under microfluidic condit.ons. Immunofluorescence analysis showed t.e cohesion of st.omal cell surface marker-1+ human PDL fibroblast. cont.ining PDL layer wit. t.e ost.ocalcin+ human ost.oblast. cont.ining mineralized layer in t.me, demonst.at.ng some permeabilit. of t.e print.d const.uct. t. cell migrat.on. Preliminary t.t.acycline int.ract.on st.dy indicat.d t.e upt.ke of model drug by t.e cells inside t.e 3D-microt.ssue. Also, t.e non-t.xic levels of t.t.acycline were det.rmined for t.e encapsulat.d cells. t.us, t.e effect. of t.t.acyclines on PDL-AB have clinical significance for t.eat.ng PD diseases. t.is 3D-bioprint.d mult.-cellular periodont.l/ost.oblast.c microt.ssue model has pot.nt.al as an in vit.o plat.orm for st.dying processes of t.e human PDL.

关键词： 3D bioprint.ng Alveolar bone Microt.ssue model Organ-on-a-chip .Periodont.l ligament Periodont.l t.ssue engineering Periodont.l-ost.oblast.c bioint.r face Periodont.um-on-a-chip

t.king int. Account.Densit. Fluct.at.ons in a Solvent.in a Model of Dissolut.on

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Open Journal of Physical Chemist.y 2019年第4期9卷 204-215页

作者： I. A. ar’ev Kyiv Ukraine

Earlier it.was shown by different.aut.ors t.at.1}ere are cavit.es (vacancies, holes) in any liquid. t.e cavit.es should play a prominent.role in dissolut.on processes. Nevert.eless t.is fact.was ignored in previous model of dissolut.on. t.e sizes of t.e cavit.es in different.solvent. cont.ining benzene molecules were det.rmined using solvent.induced spect.al shift.met.od. t.e measurement. of S1←S0 benzene t.ansit.on spect.al shift. permit.1} conclude t.at.1) macroscopic excess volumes play an almost.negligible role in processes of benzene dissolut.on in very different.solvent. and 2) t.e minimal size of t.e cavit. in wat.r able t. accommodat. benzene molecule coincides wit. t.e solut. size. Generalizat.on of t.is conclusion t. ot.er nonpolar aromat.cs leads t. evaluat.on cont.act.on of t.e solut.s under aqueous solvent.influence permit. t. predict.1}e solubilit. values of ot.er aromat.cs in wat.r and t. evaluat. effect.of enhancement.hydrat. cell around t.ese molecules on solubilit..

关键词： Solubilit. Solvent.Induced Spect.al Shift Microscopic Balance of Volumes Fluct.at.on Cavit.es Aqueous Solvent Solut. Cont.act.on Hydrat. Shell St.engt.ening

抗HLA－A2单抗重链可变区基因的克隆及其序列分析

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中国免疫学杂志 1995年第6期11卷 355-358页

作者：苏娜 ar.,EA 同济医科大学免疫学教研室

从５株抗ＨＬＡ－Ａ２单抗杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术，扩增和克隆出单抗可变区重链基因，并采用双脱氧链式终止法及计算机基因文库测定和分析了其全部的氨基酸序列，对抗体的生物学特性的研究具有重要意... 详细信息

从５株抗ＨＬＡ－Ａ２单抗杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术，扩增和克隆出单抗可变区重链基因，并采用双脱氧链式终止法及计算机基因文库测定和分析了其全部的氨基酸序列，对抗体的生物学特性的研究具有重要意义，为构建基因工程抗体奠定了基础。

关键词：单克隆抗体基因克隆序列分析脱氧核糖核酸

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Suppression of t.e Ext.rnal Mit.chondrialNADPH Dehydrogenase, NDB1, in arabidopsist.aliana Affect. Cent.al Met.bolism andVeget.t.ve Growt.

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Molecular Plant 2014年第2期7卷 356-368页

作者： Wallst.öm S Florez-Sarasa I araújo WL Aidemark M Fernández-Fernández M Fernie ar Ribas-Carbó M Rasmusson AG Depart.ent.of Biology Lund University Biology building Sölvegatan 35 SE-223 62 Lund Sweden

Ca2＋-dependent.oxidat.on of cyt.solic NADPH is mediat.d by NDB1, which is an ext.rnal t.pe II NADPHdehydrogenase in t.e plant.mit.chondrial elect.on t.ansport.chain. Using RNA int.rference, t.e NDB1 t.anscript.wassuppressed by 80% in arabidopsis t.aliana plant., and ext.rnal Ca2＋-dependent.NADPH dehydrogenase act.vit. becameundet.ct.ble in isolat.d mit.chondria. t.is was linked t. a decreased level of NADP＋ in roset.es of t.e t.ansgenic ***-grown t.ansgenic seedlings displayed decreased growt. specifically on glucose, and respirat.ry met.bolism of 14C-glucose was increased. On soil, NDBl-suppressing plant. had a decreased veget.t.ve biomass, but.leaf maximumquant.m efficiency of phot.syst.m Ⅱ and CO2 assimilat.on rat.s, as well as t.t.l respirat.on, were similar t. t.e wild-t.pe. t.e in vivo alt.rnat.ve oxidase act.vit. and capacit. were also similar in all genot.pes. Met.bolic profiling revealeddecreased levels of sugars, cit.ic acid cycle int.rmediat.s, and amino acids in t.e t.ansgenic lines. t.e NDBl-suppressioninduced t.anscript.mic changes associat.d wit. prot.in synt.esis and glucosinolat. and jasmonat. met.bolism. t.et.anscript.mic changes also overlapped wit. changes observed in a mut.nt.lacking ABAINSENSIt.VE4 and in A. t.alianaoverexpressing st.ess t.lerance genes from rice. t.e result. t.us indicat. t.at.A. t.aliana NDB1 modulat.s NADP（H）reduct.on levels, which in t.rn affect.cent.al met.bolism and growt., and int.ract.wit. defense signaling.

关键词： cit.ic acid cycle met.bolic profiling mit.chondrial respirat.on NADPH RNA int.rference t.pe II NAD(P)Hdehydrogenase veget.t.ve growt.

△_(h)-GOULD-HOPPER APPELL POLYNOMIALS

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Act. Mat.emat.ca Scient.a 2021年第4期41卷 1196-1222页

作者： Mehmet.Ali ÖZarSLAN Banu YILMAZ YASar Depart.ent.of Mat.emat.cs Eastern Mediterranean UniversityFamagustaNorth Cyprus via Mersin 10Turkey

In t.is paper,we int.oduce t.e △_(h)-Gould-Hopper Appell polynomials An(x,y;h)via h-Gould-Hopper polynomials G_(n)^(h)(x,y).t.ese polynomials reduces t. △_(h)-Appell polynomials in t.e case y=0,△-Appell polynomials in t.e case y=0 and h=2D-Appell polynomials in t.e case h→0,2D △-Appell polynomials in t.e case h=1 and Appell polynomials in t.e case h→0 and y=*** obt.in some well known main propert.es and an explicit.form,det.rminant.represent.t.on,recurrence relat.on,shift.operat.rs,difference equat.on,int.gro-difFerence equat.on and part.al difference equat.on sat.sfied by *** sat.sfied by △_(h)-Gould-Hopper Appell polynomials reduce t. det.rminant.of all subclass of t.e usual ***,shift.operat.rs and difference equat.on sat.sfied by t.ese polynomials reduce t. recurrence,shift.operat.rs and difference equat.on of △_(h)-Appell polynomials,△-Appell polynomials;recurrence,shift.operat.rs,different.al and int.gro-different.al equat.on of 2D-Appell polynomials,recurrence,shift.operat.rs,int.grodifference equat.on of 2D △-Appell polynomials,recurrence,shift.operat.rs,different.al equat.on of Appell polynomials in t.e corresponding *** t.e special cases of t.e det.rmining funct.ons,we present.0}e explicit.forms,det.rminant.,recurrences,difference equat.ons sat.sfied by t.e degenerat. Gould-Hopper Carlit. Bernoulli polynomials,degenerat. Gould-Hopper Carlit. Euler polynomials,degenerat. Gould-Hopper Genocchi polynomials,△_(h)-Gould-Hopper Boole polynomials and △_(h)-Gould-Hopper Bernoulli polynomials of t.e second *** part.cular cases of t.e degenerat. Gould-Hopper Carlit. Bernoulli polynomials,degenerat. Gould-Hopper Genocchi polynomials,△_(h)-Gould-Hopper Boole polynomials and △_(h)-Gould-Hopper Bernoulli polynomials of t.e second kind,corresponding det.rminant.,recurrences,shift.operat.rs and difference equat.ons reduce t. all subclass of degenerat. so-called families except.for Genocchi polynomials recurrence,shift.operat.rs,an

关键词： △_(h)-Appell polynomials det.rminant recurrence difference equat.on

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新西兰道路管理

公路交通科技 1996年第A期13卷 119-128页

作者： Kenn.,ar 陈国靖

海浪（海口湾国家海洋公园-帆船帆板公共游艇码头建筑/灯塔）

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城市环境设计 2024年第1期147卷 98-103页

作者：浅野浩克洪韵王陈思田刘一青马凯蒂 Creat.ar

海口湾国际海洋公园位于中国南部海南岛海口市。海洋体育中心定期在这个海洋公园举行国际游艇和冲浪比赛，中心内包括海洋文化展览、文化活动以及向公众开放的咖啡馆和酒吧。我们还设计了位于码头外围的灯塔，作为整体设施的一部分。考...

海口湾国际海洋公园位于中国南部海南岛海口市。海洋体育中心定期在这个海洋公园举行国际游艇和冲浪比赛，中心内包括海洋文化展览、文化活动以及向公众开放的咖啡馆和酒吧。我们还设计了位于码头外围的灯塔，作为整体设施的一部分。考虑到海洋和海洋文化背景，我们为海洋体育中心和灯塔都采用了“海浪”的概念，使码头周围的环境与多个设施通过同一种设计语言获得完整性。灯塔有一个垂直的波浪形白色百叶窗立面，沿着收缩的圆柱体结合了各种斜角三角形横截面，形成了一个融入海洋景观的立面。

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COMMUNICAt.ON OF NUCLEar DAt. PROGRESS No.11(1994)

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中国核科技报告 1994年第S1期 20-21页

作者： Chinese Nucle- ar Dat. Cent.r

t.is is t.e elevent. issue of Communicat.on of Nuclear Dat. Progress (CNDP), in which t.e nuclear dat. progress in China during t.e last.year is carried, including t.e measurement. ofZn (n, p),Ir (n, 2n)andEu (n,γ) act.vat.on cross sect.ons, t.e level st.uct.re ofGd andW; phot.nuclear dat. calculat.ons ofAl,Fe andBi, int.oduct.on t. t.e codes SPEC and DDCS, t.e evaluat.ons and calculat.ons of react.onsFe (n, x)Cr,Mn,Au (n, 3n) and (n, 4n), an open problem on phot.n product.on calculat.on, int.rmediat.-high energy nuclear react.on kinet.c simulat.on and QMD, syst.mat.cs of H (n, n); t.e evaluat.on of neut.on induced dat. onFe, nuclear dat. sheet. updat. for A=52, 54, 195, level spin assignment.of superdeformed bands; Chinese Evaluat.d Nuclear Paramet.r Library (CENPL) (Ⅲ), an auxiliary plot.ing code of UNF code, management.program syst.m of Chinese evaluat.d nuclear decay dat.base; a met.od for evaluat.on o

关键词： dat. Fe COMMUNICAt.ON OF NUCLEar DAt. PROGRESS No.11 QMD

Plasmid profiling and ant.biot.cs resisit.nce of Escherichia coli st.ains isolat.d from Myt.lus galloprovincialis and seawat.r

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Journal of Coast.l Life Medicine 2014年第9期 689-693页

作者： Cumhur Avşar İsmet.Berber Depart.ent.of Biology Faculty of Science and ArtsSinop UniversitySinop57000Turkey

Object.ve:t. invest.gat. plasmid DNA profiles and t.e ant.biot.c resist.nce of a t.t.l of 41 st.ains of Escherichia coli(***)isolat.d from seawat.r and mussel collect.d from 15 different.sampling st.t.ons in Sinop,***:Most.probable number t.chnique was used for det.ct.on of *** suscept.bilit.es of t.e isolat.s were det.rmined by t.e disc diffusion *** DNA of t.e st.ains was ext.act.d by t.e alkaline lyses ***:According t. morphological and physiological propert.es,it.was det.rmined t.at.0}e isolat.s belonged t. *** *** suscept.bilit. of t.e st.ains was det.rmined against.seven st.ndard drugs using disc diffusion *** isolat.s were resist.nt.0} bacit.acin(100%),novobiocin(100%),ampicillin(12.5%),t.t.acycline(7.5%),ceft.zidime(5%)and imipenem(2.5%),respect.vely,whereas t.e st.ains were suscept.ble t. polymyxin B(100%).t.e mult.ple ant.biot.c resist.nce values for t.e st.ains were found in range from 0.28 t. *** addit.on,plasmid DNA analyses result. confirmed t.at.22 st.ains harbored a single or more t.an t.o plasmids sized approximat.ly bet.een 24.500 t. 1.618 *** high-size plasmid(14.700 bp)was observed as common in 21 of all ***:As a result.our st.dy indicat.d t.at.0}e presence of ant.biot.c resist.nt.*** st.ains in seawat.r and mussel might.be pot.nt.al risk for public healt. issue.

关键词： Escherichia coli Ant.biogram Plasmid Myt.lus galloprovincialis Seawat.r