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Cross-linked alginate edible coatings incorporated with hexyl acetate: Film characteristics and its application on fresh-cut rose apple

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Food Bioscience 2023年第2期52卷 759-767页

作者： Nhung Thi Cam Duong apiradee uthairatanakij Natta Laohakunjit Pongphen Jitareerat Nattapon Kaisangsri Division of Postharvest Technology School of Bioresources and TechnologyKing Mongkut’s University of TechnologyThonburiThailand Division of Biochemical Technology School of Bioresources and TechnologyKing Mongkut’s University of TechnologyThonburiThailand Pilot Plant Development and Training Institute King Mongkut’s University of TechnologyThonburiThailand

Edible coatings,as carriers of antimicrobial compounds,have been proposed as an alternative method to preserve fresh-cut *** research focused to improve the shelf life of fresh-cut rose apples by applying an edible coating based on alginate cross-linked with calcium chloride(SAC)-enriched hexyl acetate(HA)as a natural antimicrobial *** SAC with HA in 0.01,0.03,and 0.05%(v/v)(SAC-HA)solution was evaluated for its film properties and applied to fresh-cut rose apples,and compared with the unmodified SAC *** quality and microbial contamination were determined every 2 days during 10 days of storage at 4℃.The film thickness,tensile strength,and elongation at break increased with the high concentrations of HA *** containing 0.03%HA had lower oxygen permeability(from 1.57 to 0.04×10^(-7) cm^(3)μm m^(-2) day^(-1) kPa^(-1)),as well as water vapor permeability(from 0.03 to 0.01 g mm m^(-2) day^(-1) kPa^(-1)),compared to the unmodified SAC ***,the greater coating uniformity and thickness were supported by scanning electron microscopy and Fourier transform infrared *** developed edible SAC coatings with 0.03%HA successfully preserved the physicochemical quality and extended the shelf life of fresh-cut rose apples for 10 days at 4℃ by retarding the respiration rate,inhibiting browning,and preventing microbial ***,the using of SAC-HA in fresh-cut products is a promising and safe preservation technique to extend shelf life.

关键词： Film properties Browning Antimicrobial Rose apple Postharvest quality

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Molecular genome tracking of East,Central and South African genotype of Chikungunya virus in South-east Asia between 2006 and 2009

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 535-540页

作者： Kamol Suwannakarn apiradee Theamboonlers Yong Poovorawan Center of Excellence in Clinical Virology Faculty of MedicineChulalongkorn University and King Chulalongkorn Memorial HospitalBangkokThailand

Objective:To understand the epidemiology of the East,Central and South African(ECSA) genotype of Chikungunya virus(CHIKV) in terms of emerging and re-emerging infections,this study has been aimed at investigating the evolutionary parameters,genomic signatures and molecular tracking of the CHIKV ECSA genotype in South-east Asia and coastal areas of the Indian Ocean between 2006 and 2009 by using phylogenetie analysis and the Bayesian Markov Chain Monte Carlo(BMCMC) evolutionary ***:Nearly complete genome sequences of 53 CHIKV isolates from all genotypes were subjected to phylogenetie analysis and evolutionary parameter *** amino acids of 67 of ECSA genotype during 2006 to 2009 were compared for finding molecular signature *** ECSA genotype signatures were visualized to find the possible transmission root was projected onto a geographic ***: Phylogenetie analysis showed the ECSA genotype was divided into 2 *** first group comprises viruses from India and Southeast Asian *** second group consists of strains typically circulating in Sri Lanka in *** evolutionary parameters of these groups depicted the time of the most recent common ancestor at approximately 7.5 years *** genomic signatures revealed the positions of amino acid variation in each ***:The molecular evolution projected onto a geographical map showed the routes of CHIKV transmission from 2006 to *** tracking will assist in understanding transmission routes, epidemiology and molecular evolution of CHIKV.

关键词： Chikungunya virus East Central and South African genotype Genomic signature Evolutionary parameter

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Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus

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Asian Pacific Journal of Tropical Medicine 2012年第5期5卷 342-346页

作者： Piyathida Pongsiri Kesmanee Praianantathavorn apiradee Theamboonlers Sunchai Payungporn Yong Poovorawan Center of Excellence in Clinical Virology Department of Pediatrics Faculty of Medicine.Chulalongkorn UniversityBangkok 10330Thailand Department of Biochemistry Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Center of Excellence in Clinical Virology Department of PediatricsFaculty of Medicine.Chulalongkorn UniversityBangkok 10330Thailand Department of Biochemistry Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand Center of Excellence in Clinical Virology Department of PediatriesFaculty of MedicineChulalongkorn UniversityBangkok 10330Thailand

Objective:To develop diagnostic test for detection chikungunya virus(CHIKV and Dengue virus (DENV) ***:We have performed a rapid,accurate laboratory confirmative method to simultaneously detect,quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time ***:The assay’s sensitivity was 97.65%,specificity was 92.59% and accuracy was 95.82%when compared to conventional ***,there was no cross-reaction between CHIKV,DENV,Japanese encephalitis virus,hepatitis C,hepatitis A or hepatitis E ***:This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

关键词： Multiplex Real-time RT-PCR Chikungunya virus Dengue virus

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Risk factors and molecular characterization of acute sporadic symptomatic hepatitis E virus infection in Thailand

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Asian Pacific Journal of Tropical Medicine 2014年第9期7卷 709-714页

作者： Kittiyod Poovorawan Salyavit Jitmitrapab Sombat Treeprasertsuk Thanunrat Thongmee apiradee Theamboonlers Pisit Tangkijvanich Piyawat Komolmit Yong Poovorawan Division of Gastroenterology Department of MedicineChulalongkorn University Center of Excellence in Clinical Virology Chulalongkorn University Department of Biochemistry Faculty of MedicineChulalongkorn University

Objective:To report clinical outcomes and viral genotypes of acute symptomatic hepatitis E virus(HEV)infection in ***:Forty patients with acute symptomatic HEV infection were recruited during ***,demographic and laboratory data were *** was accomplished by detection of anti-HEV IgM and/or HEV RNA in the serum or *** genotypes were classified by direct sequencing of RT-PCR products and phylogenetic ***:The high risk group,comprising immune-compromised,liver cirrhosis and very elderly(>80 years)patients(17 cases),had higher levels of serum alkaline phosphatase at presentation compared with the low risk *** fatal cases resulted from acute hepatitis E in the high risk group,initial clinical presentation did not show statistically significant *** six cases(6/40),the virus could be detected in serum or stool by RT-PCR and *** molecular characterization,the viruses were classified as HEV genotype 3f and were in the same cluster as Thai swine ***:Our data showed that acute HEV infection has various clinical presentations and *** levels of serum alkaline phosphatase were observed in high risk *** isolated viruses were identified as HEV genotype 3f possibly originating from swine.

关键词： Hepatitis E Hepatitis E virus Genotype 3f Thailand Risk factors

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Prediction of cyanotic and acyanotic congenital heart disease using machine learning models

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World Journal of Clinical Pediatrics 2024年第4期13卷 15-24页

作者： Sana Shahid Haris Khurram apiradee Lim Muhammad Farhan Shabbir Baki Billah Department of Statistics Bahauddin Zakariya UniversityMultan 60000PunjabPakistan Department of Mathematics and Computer Science Faculty of Science and TechnologyPrince of Songkla UniversityPattani CampusPattani 94000Thailand Department of Science and Humanities National University of Computer and Emerging SciencesChiniot-Faisalabad CampusChiniot 35400PunjabPakistan Department of Cardiology Chaudhary Pervaiz Elhai Institute of CardiologyMultan 60000PunjabPakistan School of Public Health and Preventive Medicine Monash UniversityMelbourne 3000VictoriaAustralia

BACKGROUND Congenital heart disease is most commonly seen in neonates and it is a major cause of pediatric illness and childhood morbidity and *** To identify and build the best predictive model for predicting cyanotic and acyanotic congenital heart disease in children during pregnancy and identify their potential risk *** The data were collected from the Pediatric Cardiology Department at Chaudhry Pervaiz Elahi Institute of Cardiology Multan,Pakistan from December 2017 to October 2019.A sample of 3900 mothers whose children were diagnosed with identify the potential *** machine learning models were compared,and the best-fitted model was selected using the area under the curve,sensitivity,and specificity of the *** Out of 3900 patients included,about 69.5%had acyanotic and 30.5%had cyanotic congenital heart *** had more cases of acyanotic(53.6%)and cyanotic(54.5%)congenital heart disease as compared to *** odds of having cyanotic was 1.28 times higher for children whose mothers used more fast food frequently during *** artificial neural network model was selected as the best predictive model with an area under the curve of 0.9012,sensitivity of 65.76%,and specificity of 97.23%.CONCLUSION Children having a positive family history are at very high risk of having cyanotic and acyanotic congenital heart *** are more at risk and their mothers need more care,good food,and physical activity during *** best-fitted model for predicting cyanotic and acyanotic congenital heart disease is the artificial neural *** results obtained and the best model identified will be useful for medical practitioners and public health scientists for an informed decision-making process about the earlier diagnosis and improve the health condition of children in Pakistan.

关键词： Congenital heart disease Cyanotic heart disease Acyanotic heart disease Logistic regression model Artificial neural network

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The diurnal variation pattern of choroidal thickness in macular region of young healthy female individuals using spectral domain optical coherence tomography

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 561-566页

作者： Mcng Zhao Xiu-Fcn Yang Xuan Jiao apiradee Lim Xue-Tao Ren Torkel Snellingen Ning-Pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology and Visual Sciences Key LaboratoryBeijing 100730China Department of Ophthalmology Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Mathematics and Computer Science Facultyof Science and TechnologyPrince of Songkla UniversityPattani CampusMuang Pattani 90112Thailand Sekwa Institute of Medical Science Beijing 100088China

AIM： To investigate the pattern of diurnal variations of choroidal thickness of macular region of healthy individuals. ~ METHODS： A prospective study of 32 healthy female subjects was conducted. Each subject underwent 1） a questionnaire on daily schedule, 2） the Pittsburgh Sleep Quality Index questionnaire （PSQI）, and 3） ocular examinations including an eye dominance test, fundus photography, and sequential optical coherence tomography （OCT） imaging, on two separate days at five fixed 3h time intervals. Choroidal thickness was measured by two masked graders. RESULTS： A significant diurnal variation of choriodal thickness at fovea （P〈0.001）, at 500 μm nasal （P〈0.001）, temporal to fovea （P=0.01） or 1500 μm nasal to fovea （P= 0.001） was observed. The median choroidal thickness peaked at 11：00 at fovea （P=0.01）, at 500μm nasal （P = 0.009） and temporal （P=0.03） to fovea. The median amplitude of foveal choroidal thickness was 20.5 pm （13, 31） and 20.0 μm （12.5, 28.2） for the first and second series of measurements, respectively. The greater amplitude of foveal choroidal thickness was associated with thickner initial foveal choroidal thickness [ 0.05 （0.03, 0.08）, P= 0.01], dominant eye 10.51 （4.02, 14.60）, P=0.04] in the multivariate linear regression. CONCLUSION： Our data show a significant diurnal variation of the choroidal thickness at fovea, at 500 μm nasal and temporal to fovea and 1500 μm nasal to fovea. Thicker initial foveal choroidal thickness and being dominant eye may influence the amplitude of foveal choroidal thickness.

关键词： choroidal thickness diurnal variation optical coherence tomography

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Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand

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World Journal of Gastroenterology 2005年第15期11卷 2238-2243页

作者： Pisit Tangkijvanich Varocha Mahachai Piyawat Komolmit Juthatip Fongsarun apiradee Theamboonlers Yong Poovorawan Department of Biochemistry Department of Medicine The National Blood Center Viral Hepatitis Research Unit

AIM: The role of hepatitis B virus (HBV) genotypes on the clinical features and prognosis of patients with hepatocellular carcinoma (HCC) is currently unknown. The aim of the present study was to evaluate the distribution of HBV genotypes and their clinical relevance in Thai ***: HBV genotypes were determined by PCR-RFLP in stored sera of 93 asymptomatic carriers, 103 patients with chronic hepatitis, 60 patients with cirrhosis and 76patients with HCC. The clinical data were analyzed in relation to the HBV ***: HBV genotypes C and B were predominant in Thailand, accounting for 73% and 21%, respectively. The distributions of genotypes B and C were similar in HCC patients compared to the other groups. Genotype C was significantly more common in HCC patients who were under 40 years old than genotype B (18% vs 0%, P= 0.03), but was significantly less common in patients older than 60 years (26% vs 56.5%, P= 0.01). The positive rate of hepatitis B e antigen (HBeAg) in patients with genotype C was significantly higher than that in patients with genotype B (71.6% vs 44.4%, P = 0.03 in chronic hepatitis; 56.8% vs 11.1%,P = 0.01 in cirrhosis). There were no differences between HCC patients with genotypes B and C regarding tumor staging by CLIP criteria and the overall median survival. Multivariate analyses showed that HBV genotype was not an independent prognostic factor of survival in HCC ***: Patients with genotype C had a higher positive rate of HBeAg and exhibited earlier progression of cirrhosis and HCC than those with genotype B. However,there were no differences in the risk of developing HCC and its prognosis between patients with these genotypes.

关键词： HBV Genotype Hepatocellular carcinoma

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Impact of alcohol consumption on treatment outcome of hepatocellular carcinoma patients with viral hepatitis who underwent transarterial chemoembolization

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World Journal of Hepatology 2022年第6期14卷 1162-1172页

作者： Attapon Rattanasupar Arunchai Chang Tanaporn Prateepchaiboon Nuttanit Pungpipattrakul Keerati Akarapatima apiradee Songjamrat Songklod Pakdeejit Varayu Prachayakul Teerha Piratvisuth Division of Gastroenterology Department of Internal MedicineHatyai HospitalHatyai 90110SongkhlaThailand Department of Internal Medicine Hatyai HospitalHatyai 90110SongkhlaThailand Division of Intervention Radiology Department of RadiologyHatyai HospitalHatyai 90110SongkhlaThailand Siriraj Gastrointestinal Endoscopy Center Division of GastroenterologyDepartment of Internal MedicineFaculty of MedicineSiriraj HospitalMahidol UniversityBangkoknoi 10700BangkokThailand NKC Institute of Gastroenterology and Hepatology Songklanagarind HospitalFaculty of MedicinePrince of Songkla UniversityHatyai 90110SongkhlaThailand

BACKGROUND Alcohol consumption increases the risk of hepatocellular carcinoma(HCC)in patients with pre-existing liver disease,including viral ***,studies on the impact of alcohol consumption on the outcomes of HCC are *** hypothesized that alcohol had an additional effect with chronic viral hepatitis infection on treatment outcomes after transarterial chemoembolization(TACE)in patients with intermediate-stage HCC(Barcelona Clinical Liver Cancer[BCLC]-B).AIM To evaluate the additional effect of alcohol on treatment outcomes of TACE among HCC patients with viral *** This study,conducted at Hatyai Hospital in Thailand,included HCC patients over 18 years of age with chronic viral *** of HCC patients with viral hepatitis classified as BCLC-B who underwent TACE as the first treatment modality between 2014 and 2019 were retrospectively *** with chronic viral hepatitis only were categorized under group A,and those with chronic viral hepatitis and concurrent alcohol consumption were categorized under group *** groups were compared,and the Cox proportional-hazards model was used to identify the survival-influencing *** Of the 69 patients,53 were categorized in group A and 16 in group *** were no statistically significant differences in tumor characteristics between the two patient ***,Group A had a statistically significantly higher proportion of complete response(24.5%vs 0%,P=0.030)and a higher median survival rate(26.2 mo vs 8.4 mo;log-rank P=0.012)compared to group *** associated with decreased survival in the proportional-hazards model included alcohol consumption(hazards ratio[HR],2.377;95%confidence interval[CI],1.109-5.095;P=0.026),presence of portal hypertension(HR,2.578;95%CI,1.320–5.037;P=0.006),largest tumor size>5 cm(HR,3.558;95%CI,1.824-6.939;P100 ng/mL(HR,2.536;95%CI,1.377-4.670;P=0.003).CONCLUSION In HCC BCLC B patients

关键词： Alcohol misuse Chronic viral hepatitis Hepatocellular carcinoma Risk factor Survival Transarterial chemoembolization

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Association of C(-106)T Polymorphism in Aldose Reductase Gene with Diabetic Retinopathy in Chinese Patients with Type 2 Diabetes Mellitus

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Chinese Medical Sciences Journal 2014年第1期29卷 1-6页

作者： Yu Deng Xiu-fen Yang Hong Gu apiradee Lim Munkhtulga Ulziibat Torkel Snellingen Jun Xu Kai Ma Ning-pu Liu Department of Ophthalmology Fu Xing HospitalCapital Medical University Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology and Visual Sciences Key Laboratory Department of Mathematics and Computer Science Faculty of Science and TechnologyPrince of Songkla UniversityPattani Campus Sekwa Eye Hospital

Objective To identify the possible association between C（-106）T polymorphism of the aldose reductase （ALR） gene and diabetic retinopathy （DR） in a cohort of Chinese patients with type 2 diabetes mellitus （T2DM）. Methods From November 2009 to September 2010, patients with T2DM were recruited and assigned to DR group or diabetic without retinopathy （DWR） group according to the duration of diabetes and the grading of 7-field fundus color photographs of both eyes. Genotypes of the C（-106）T polymorphism （rs759853） in ALR gene were analyzed using the MassARRAY genotyping system and an association study was performed. Results A total of 268 T2DM patients （129 in the DR group and 139 in the DWR group） were included in this study. No statistically significant differences were observed between the 2 groups in the age of diabetes onset （P=0.10） and gender （P=0.78）. The success rate of genotyping for the study subjects was 99.6% （267/268）, with one case of failure in the DR group. The frequencies of the T allele in the C（-106）T polymorphism were 16.0% （41/256） in the DR group and 19.4% （54/278） in the DWR group （P=0.36）. There was no signit^cant difference in the C（-106）T genotypes between the 2 groups （P=0.40）. Compared with the wild-type genotype, odds ratio （OR） for the risk of DR was 0.7 （95% CI, 0.38-1.3） for the heterozygous CT genotype and 0.76 （95% CI, 0.18-3.25） for the homozygous TT genotype. The risk of DR was positively associated with microalbuminuria （OR=4.61; 95% CI, 2.34-9.05） and insulin therapy （OR=3.43; 95% CI, 1.94-6.09）. Conclusions Microalbuminuria and insulin therapy are associated with the risk of DR in Chinese patients with T2DM. C（-106）T polymorphism of the ALR gene may not be significantly associated with DR in Chinese patients with T2DM.

关键词： diabetic retinopathy aldose reductase gene single nucleotide polymorphism

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Diagnostic role of serum interleukin-18 in gastric cancer patients

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World Journal of Gastroenterology 2006年第28期12卷 4473-4477页

作者： Duangporn Thong-Ngam Pisit Tangkijvanich Rungsun Lerknimitr Varocha Mahachai apiradee Theamboonlers Yong Poovorawan Department of Physiology Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Department of Biochemistry Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Department of Medicine Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Viral Hepatitis Research Unit Department of PediatricsFaculty of MedicineChulalongkorn UniversityBangkok10330Thailand

AIM： To determine the current status in various aspects of gastric cancer patients and to find out the clinical correlation with prognostic role of serum interleukins in Thai patients. METHODS： Sixty-eight patients were enrolled in this study at King Chulalongkorn Memorial Hospital during April 2003 to May 2005. Gastric cancer was histologically proven in 51 patients and gastric ulcer in 17 patients. Serum IL-6, IL-10, IL-12, and IL-18 levels were measured by enzyme-linked immunosorbent assay （ELISA）. RESULTS： There were 26 males （55.32%） and 21 females （44.68%） with their age ranging from 33 to 85 years （mean age 64.49 ± 13.83 years）. The common presentations were weight loss （41.2%）, dyspepsia （39.2%）, and upper gastrointestinal bleeding （15.7%）. A total of 35.3% gastric cancer patients and 6.3% of gastric ulcer patients were smokers （P = 0.029）. Moreover, 32.4% of gastric cancer patients and 6.3% of gastric ulcer patients were alcoholic drinkers （P = 0.044）. Lesion location was pyloric-antrum in 39.4%, gastric body in 39.4%, upper stomach in 12.2% and entire stomach in 6.1% of the patients. H pylori infection was detected in 44.4%. The poorly-differentiated adenocarcinoma was the most common pathologic finding （60.7%）. Surgical treatment was performed in 44.1% patients （total gastrectomy in 5.9%, subtotal gastrectomy in 32.4% and palliative bypass surgery in 5.9%）. Systemic chemotherapy was given as an adjuvant therapy in 8.8% patients. Carcinomatosis peritoneii were found in 18.8% patients. The mean survival time was 13.03 ± 9.75 mo. The IL-18 level in gastric cancer patient group （58.54 ± 43.96 pg/mL） was significantly higher than that in gastric ulcer patient group （30.84± 11.18 pg/mL）（P = 0.0001）（95% CI was 42.20, 13.19）. The cut point of IL-18 for diagnosis of gastric cancer was 40 pg/mL, the positive predictive value was 92.31%. The IL-6 level in gastric cancer patients with distant metastasis （20.21 ±9.37 pg/mL） was significantly higher than that in thos

关键词： Interleukin Gastric cancer Diagnostic marker

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