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脂质转移蛋白SiLTP1基因参与谷子耐盐响应初探

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作物学报 2025年第1期51卷 58-67页

作者：孟凡花刘敏沈傲刘炜山东省农业科学院农作物种质资源研究所山东济南250100 上海应用技术大学生态技术与工程学院上海201418

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁... 详细信息

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁迫过程。前期本课题组克隆到一个谷子中编码脂质转移蛋白的基因SiLTP1,本研究通过构建其原核表达载体和植物双元过表达载体,获得了该基因的原核表达蛋白及同源转化过表达阳性植株,经进一步筛选获得4个纯合株系。体外耐盐性试验显示, SiLTP1的原核表达蛋白具有一定耐盐性;转基因植株苗期耐盐生理指标测定显示,SiLTP1过表达植株在遭受盐胁迫时,植株体内MDA积累减少,抗氧化酶含量升高,可积累更少的过氧化氢,从而降低胁迫对植株的氧化损伤,显示基因过表达植株具有更好的耐盐性。本研究结果初步揭示, SiLTP1可正向调控谷子的耐盐性,其在谷子耐盐抗逆方面具有潜在功能,为耐盐谷子品种改良及新品种培育提供了理论基础和候选基因资源。

关键词：脂质转移蛋白 SiLTP1 盐胁迫谷子耐盐响应

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Comparative study on the reaction of methane over a ZnO bed in the absence and presence of CO_2

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Journal of Natural Gas Chemistry 2008年第1期17卷 81-86页

作者： Xianquan ao hua Wang Yonggang Wei Faculty of Materials and Metallurgy Engineering Kunming University of Science and Technology Kunming 650093 Yunnan China Faculty of Chemical Engineering Guizhou University Guizhou 550003 Guiyang China

The reaction of zinc oxide with methane in the absence and presence of CO2 were theoretically and experimentally investigated using HSC Chemistry 5.1 software and a fixed bed reactor, respectively. In the absence of CO2 at 1193 K, the reduction of ZnO was accompanied with methane cracking, and metallic zinc, CO, and H2 were the main reaction products. This system could be utilized for the co-production of metallic zinc and synthesis gas, in which ZnO was a donor of oxygen. In the presence of CO2, ZnO plays as a catalyst in the CO2 reforming of methane and produces syngas with the average H2/CO ratio of 0.88 at 1193 K, which was close to the total reaction theoretic value of 1. It was also found that higher temperature favored high CH4 and CO2 conversions. XRD technique was used to characterize the ZnO species. The result showed that there were no differences in the peak profiles of the XRD patterns of the ZnO powder obtained before and after passing the CH4/CO2 mixed gases for 6 h at 1193 K. It is suggested that ZnO functions as a catalyst according to the redox cycle and metallic zinc plays the role of intermediate product in this process.

关键词： methane synthesis gas, ZnO CO2 reforming catalysis

Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures

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Chinese Chemical Letters 2021年第2期32卷 783-786页

作者： hua chai Peng Miao Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of SciencesSuzhou 215163China Jinan Guokeyigong Science and Technology Development Co. Ltd.Ji'nan 250103China

Circulating tumor DNA(ctDNA) refers to a class of acellular nucleic acids carrying genetic features of primary tumor,which can be regarded as a promising noninvasive biomarker for cancer *** development of ctDNA assay is an important component of liquid *** this study,we have fabricated a novel electrochemical strategy for ultrasensitive detection of ctDNA combining the merits of strand displacement amplification and DNA *** DNA triangular prism is firstly selfassembled and modified on the electrode *** target initiated strand displacement polymerization reaction,the generated DNA product helps the formation of three-way junction nanostructure on triangular prism,which localizes electrochemical *** carefully investigating the electrochemical responses,the limit of detection(LOD) for ctDNA assay as low as 48 amol/L is *** proposed electrochemical biosensor shows great potential for clinical applications.

关键词： Circulating tumor DNA Biosensor DNA triangular prism DNA three-way junction Strand displacement polymerization

Thermal transport mechanism of electrons and phonons in pristine and defective HfB_(2)

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Rare Metals 2023年第11期42卷 3651-3661页

作者： ao Wang Shou-Hang Li hua Bao Global Institute of Future Technology Shanghai Jiao Tong UniversityShanghai200240China University of Michigan-Shanghai Jiao Tong University Joint Institute Shanghai Jiao Tong UniversityShanghai200240China College of Mechanical Engineering Donghua UniversityShanghai201620China

Hafnium diboride(HfB_(2))is an important metallic ceramic that works in harsh environments,due to its high strength and thermal *** the thermal conductivity of HfB_(2) has been measured,the experimental results are ***,the thermal transport mechanism of HfB_(2) is not well *** this work,we study the thermal transport in both pristine and defective HfB_(2) from first-principles *** the pristine HfB_(2),the room-temperature thermal conductivities are 175.0 and 157.7 W·m^(-1)·K^(-1)on a-and c-axes,respectively,where the contributions from electron and phonon are *** Lorenz number is significantly smaller than the Sommerfeld value and shows a temperature dependence,which demonstrates that the Wiedemann-Franz law cannot be used to estimate electronic thermal *** phonon-isotope and the phonon-electron scattering are non-negligible compared to the phonon-phonon *** the defective HfB_(2),the grain size effects are negligible with length scales larger than 1μ*** pore can limit thermal conductivity when its occupancy is larger than 10%.The vacancy is found to induce scattered results in *** phonon thermal conductivity significantly reduces even with only 1%vacancy,while the electronic thermal conductivity is not sensitive to the *** study provides an in-depth understanding of the thermal transport in HfB_(2),and the revealed mechanisms provide important guidance on the design of HfB_(2)-based materials.

关键词： Thermal conductivity Electrical transport properties Boltzmann transport equation Vacancy scattering First-principles calculation

Function Projective Lag Synchronization of Chaotic Systems with Certain Parameters via Adaptive-impulsive Control

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International Journal of Automation and computing 2019年第2期16卷 238-247页

作者： Xiu-Li chai Zhi-hua Gan Institute of Image Processing and Pattern Recognition Henan University Institute of Intelligent Network System School of SoftwareHenan University

A new method is presented to study the function projective lag synchronization(FPLS) of chaotic systems via adaptive-impulsive control. To achieve synchronization, suitable nonlinear adaptive-impulsive controllers are designed. Based on the Lyapunov stability theory and the impulsive control technology, some effective sufficient conditions are derived to ensure the drive system and the response system can be rapidly lag synchronized up to the given scaling function matrix. Numerical simulations are presented to verify the effectiveness and the feasibility of the analytical results.

关键词： Function projective lag synchronization (FPLS) adaptive-impulsive chaotic systems numerical simulation Lyapunov stability theory

Reachable set modeling and engagement analysis of exoatmospheric interceptor

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Chinese Journal of Aeronautics 2014年第6期27卷 1513-1526页

作者： chai hua Liang Yangang Chen Lei Tang Guojin College of Aerospace Science and Engineering National University of Defense Technology

A novel reachable set（RS） model is developed within a framework of exoatmospheric interceptor engagement analysis. The boost phase steering scheme and trajectory distortion mechanism of the interceptor are firstly explored. A mathematical model of the distorted RS is then formulated through a dimension–reduction analysis. By treating the outer boundary of the RS on sphere surface as a spherical convex hull, two relevant theorems are proposed and the RS envelope is depicted by the computational geometry theory. Based on RS model, the algorithms of intercept window analysis and launch parameters determination are proposed, and numerical simulations are carried out for interceptors with different energy or launch points. Results show that the proposed method can avoid intensive on-line computation and provide an accurate and effective approach for interceptor engagement analysis. The suggested RS model also serves as a ready reference to other related problems such as interceptor effectiveness evaluation and platform disposition.

关键词： Engagement analysis Flight dynamics Interception Reachable set Spherical convex hull

Online Scheduling on Bounded Batch Machines to Minimize the Maximum Weighted Completion Time

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Journal of the Operations Research Society of China 2018年第3期6卷 455-465页

作者： Wen-hua Li Xing chai School of Mathematics and Statistics Zhengzhou UniversityZhengzhou 450001China Henan Key Laboratory of Financial Engineering Zhengzhou 450001China

We investigate the online scheduling problem on identical parallel-batch machines to minimize the maximum weighted completion *** this problem,jobs arrive over time and the processing times(of the jobs)are identical,and the batch capacity is *** this problem,we provide a best possible online algorithm with a competitive ratio of(√5+1)/***,when restricted to dense-algorithms,we present a best possible dense-algorithm with a competitive ratio of 2.

关键词： Scheduling Online algorithms Maximum weighted completion time Competitive ratio

How to signcrypt a message to designated group

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The Journal of China Universities of Posts and Telecommunications 2007年第4期14卷 57-63页

作者： MA Chun-bo LI Jian-hua ao Jun School of Information Security Engineering Shanghai Jiao Tong UniversityShanghai 200030 China The State Key Laboralory of Information Security Beijing 100049 China AO Jun State Key Laboratory for Radar Signal Processing Xidian UniversityShaanxi 710071 China

In an open network environment, the protection of group communication is a crucial problem. In this article, a novel broadcast group-oriented signcryption scheme is presented for group communication scenarios in distributed networks, Anyone in this scheme can signcrypt a message and distribute it to a designated group, and any member in the receiving group can unsigncrypt the ciphertext. The ciphertext and public key in the scheme are of constant size. In addition, this new scheme offers public verification of the ciphertext. This property is very important to the large-scale group communication since the gateway can filter the incorrect ciphertext and alleviate the receiver＇s workload. Finally, a proof in the random oracle model is given to show that the scheme is secure against chosen ciphertext attack and existential forgery.

关键词： broadcast signcryption designated group chosen ciphertext attack existential forgery random oracle model

CeO_2 as the Oxygen Carrier for Partial Oxidation of Methane to Synthesis Gas in Molten Salts: Thermodynamic Analysis and Experimental Investigation

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Journal of Natural Gas Chemistry 2007年第1期16卷 6-11页

作者： Yonggang Wei hua Wang Fang He Xianquan ao Chiyuan Zhang Faculty of Materials and Metallurgy Engineering Kunming University of Science and Technology Kunming 650093 Yunnan China

A new technique -- the direct partial oxidation of methane to synthesis gas using lattice oxygen in molten salts medium has been introduced. Using CeO2 as the oxygen carrier, thermodynamic data were calculated in the reaction process, and the results indicated that direct partial oxidation of methane to synthesis gas using lattice oxygen of cerium oxide is feasible in theory. In a stainless steel reactor, the effects of temperature and varying amounts of γ-Al2O3 supported CeO2 on cn4 conversion, H2 and CO selectivity, were investigated, respectively. The results show that 10% CeO2/γ-Al2O3 has the maximal reaction activity at a temperature of 865 ℃ and above, the H2/CO ratio in the gas that has been produced reaches 2 and the CH4 conversion, H2 and CO selectivity reached the following percentages： i.e. 61%, 89%, and 91% at 870 ℃, respectively. In addition, increase of reaction temperature is favorable for the partial oxidation of methane.

关键词： partial oxidation methane cerium oxide synthesis gas molten salt