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Comparative transcriptional analysis and identification of hub genes associated with wing differentiation of male in Aphis gossypii

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Journal of Cotton Research 2022年第3期5卷 243-260页

作者： HUANGFU Ningbo SHI Qingyu CHEN Lulu MA Xiaoyan ZHANG Kaixin LI Dongyang WANG Li ZHU Xiangzhen JI Jichao LUO Junyu cui Jinjie Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China College of Agronomy Xinjiang Agricultural UniversityUrumqi 830052China.

Background:Aphis gossypii Glover(Hemiptera:Aphididae),a worldwide polyphagous phloem-feeding agricultural pest,has three wing morphs(winged parthenogenetic female,gynopara,and male)in the life *** exclusive males could fly from summer hosts to winter hosts,which are essential for gene exchanges of cotton aphid populations from different hosts or ***,the molecular mechanism of wing differentiation of male in *** remains ***:Morphological observation of male *** showed that there is no distinct difference in the external morphologies of the 1st and 2nd instar *** obvious differentiation of wing buds started in the 3rd instar nymph and was visible via naked eyes in the 4th instar nymphal stage,then adult male emerged with full *** to morphological dynamic changes,the development of wings in males were divided into four stages:preliminary stage(the 1st instar to 2nd instar),prophase(the 3rd instar),metaphase(the 4th instar),anaphase(the 5th instar).Results of feeding behavior monitoring via EPG(electrical penetration graph)technology indicated that although the male cotton aphids had strong desire to feed(longer duration of C 55.24%,F 5.05%and Pd waves 2.56%),its feeding efficiency to summer host cotton was low(shorter E13.56%and E2 waves 2.63%).Dynamic transcriptome analysis of male aphid at 5 different developmental periods showed that in the 3rd instar nymph,the number of up-regulated DEGs was significant increased,and time-course gene transcriptional pattern analyses results also showed that numerous genes categorized in clusters 3,5,and 8 had the highest expressed levels,which were consistent with morphological changes of wing *** results indicate that the 3rd instar nymph is the critical stage of wing bud differentiation in ***,through pathway enrichment analysis of DEGs and WGCNA,it revealed that the neuroactive ligand-receptor interaction,Ras signaling pathway,dopaminergic synaps

关键词： Aphis gossypii EPG Wing bud differentiation Dynamic transcriptome WGCNA

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Hyperthermic intraperitoneal chemotherapy for gastric cancer with peritoneal metastasis:A multicenter propensity scorematched cohort study

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Chinese Journal of Cancer Research 2020年第6期32卷 794-803页

作者： Ziying Lei Jiahong Wang Zhi Li Baozhong Li Jiali Luo Xuejun Wang Jin Wang MingchenBa Hongsheng Tang Qingjun He Quanxing Liao Xiansheng Yang Tianpei Guan Han Liang Shuzhong cui Department of Abdominal Surgery Affiliated Cancer Hospital&Institute of Guangzhou Medical UniversityGuangzhou 510095China Department of General Surgery Affiliated Tumor Hospital of Zhengzhou UniversityTumor Hospital of Henan ProvinceZhengzhou 450008China Department of Surgery Anyang Tumor HospitalAnyang 455000China Department of Oncology Guangzhou Medical UniversityGuangzhou 510095China Department of Gastrointestinal Cancer Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center of CancerKey Laboratory of Cancer Prevention and TherapyTianjin's Clinical Research Center for CancerTianjin 300060China

Objective:Systemic chemotherapy has limited efficacy in the treatment of peritoneal metastasis(PM)in gastric cancer(GC).Hyperthermic intraperitoneal chemotherapy(HIPEC)combined with complete cytoreductive surgery(CRS)has shown promising outcomes but remains *** present study aimed to evaluate the safety and efficacy of HIPEC without CRS in GC patients with ***:This retrospective propensity score-matched multicenter cohort study included GC patients with PM treated with either chemotherapy alone(Cx group)or with HIPEC combined with chemotherapy(HIPEC-Cx group)in four Chinese high-volume gastric medical centers between 2010 and *** primary outcomes were median survival time(MST)and 3-year overall survival(OS).Propensity score matching was performed to compensate for controlling potential confounding effects and selection ***:Of 663 eligible patients,498 were *** MST in the Cx and HIPEC-Cx groups was 10.8 and 15.9 months,respectively[hazard ratio(HR)=0.71,95%confidence interval(95%CI),0.58-0.88;P=0.002].The 3-year OS rate was 10.1%(95%CI,5.4%-14.8%)and 18.4%(95%CI,12.3%-24.5%)in the Cx and HIPEC-Cx groups,respectively(P=0.017).The complication rates were *** time to first flatus and length of hospital stay for patients undergoing HIPEC combined with chemotherapy was longer than that of chemotherapy alone(4.6±2.4 d vs.2.7±1.8 d,P<0.001;14.2±5.8 d vs.11.4±7.7 d,P<0.001),*** median follow-up period was 33.2 ***:Compared with standard systemic chemotherapy,HIPEC combined with chemotherapy revealed a statistically significant survival benefit for GC patients with PM,without compromising patient safety.

关键词： Gastric cancer peritoneal metastasis hyperthermic intraperitoneal chemotherapy chemotherapy

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经典名方与中成药治疗慢性鼻窦炎的临床应用研究进展

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药物评价研究 2022年第6期45卷 1206-1212页

作者：崔栋卢炎新里程安钢总医院中医学科河南安阳455000 新里程安钢总医院西医耳鼻喉科河南安阳455000

慢性鼻窦炎属于中医理论的“鼻渊”范畴,经典名方与中成药具有疗效确切、不良反应少等优点,已经被广泛应用于该病的临床治疗中。通过梳理近10年来发表的文献,总结了常用的经典名方(麻杏石甘汤、参苓白术散、龙胆泻肝汤、千金苇茎汤、养... 详细信息

慢性鼻窦炎属于中医理论的“鼻渊”范畴,经典名方与中成药具有疗效确切、不良反应少等优点,已经被广泛应用于该病的临床治疗中。通过梳理近10年来发表的文献,总结了常用的经典名方(麻杏石甘汤、参苓白术散、龙胆泻肝汤、千金苇茎汤、养阴清肺汤、托里消毒散、温肺止流丹、黄芩滑石汤、补中益气汤、清化饮)和临床使用频次较高的中成药(鼻渊舒口服液、香菊胶囊、通窍鼻炎片、通窍鼻炎颗粒、鼻窦炎口服液、辛芩颗粒、苍耳子鼻炎胶囊),在治疗慢性鼻窦炎方面的特点与疗效,为进一步探索中医药治疗慢性鼻窦炎的作用机制提供参考。

关键词：慢性鼻窦炎中医药经典名方中成药临床应用

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The crystal structure,photocatalytic property of a new three-dimensional Cd(Ⅱ)-based metal-organic framework

The crystal structure,photocatalytic property of a new three...

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河南省化学会2018年学术年会

作者： Cai-Xia Yu Xiao-Qing cui Meng-Jiao Guo Department of Chemistry and Chemical Engineering Anyang Normal University

Solvothermal reactions of Cd(OAc)·2HO with 1,4-bis[(pyridin-3-yl)methoxy]benzene(bpmb)and benzene-1,2-dicarboxylic acid(HL)in MeOH/HO at 165°C gave rise to one new Cd(II)-based metal-organic framework[CdL(bpmb)](1).In 1,the asymmetric unit contains one Cd cation,one Lanion,half of bpmb *** Cd centre is five-coordinated by four carboxylate O atoms from two Lligands and one N atom from bpmb ligand,forming a disordered pentagonal-pyramidal coordination *** Cd centre are interlinked by Lligands to form a one dimensional[Cd(L)] *** chains are further connected by bpmb linkers,giving rise to a two dimensional network,and these networks are pillared by bpmb to afford a three-dimensional framework with one-dimensional rhombic channels with a 34678 topology(Figure 1a).The single framework of1 has large channels,they are mainly filled by the two other equivalent frameworks to form a three-fold interpenetrating *** 1 exhibits relatively good photocatalytic activity towards the degradation of methylene blue(MB)in aqueous solution under UV irradiation(Figure 1b).

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Effectiveness of intravenous r- tPA versus UK for acute ischaemic stroke: a nationwide prospective Chinese registry study

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Stroke & Vascular Neurology 2021年第4期6卷 603-609,I0060-I0066页

作者： Xinhong Wang Xiaoqiu Li Yuming Xu Runhui Li Qingcheng Yang Yong Zhao Fengyun Wang Baoying Sheng Runqing Wang Shaoyuan Chen Lihua Wang Liying Shen Xiaowen Hou Yu cui Duolao Wang Bin Peng Craig S Anderson Huisheng Chen On behalf of INTRECIS Investigators Neurology General Hospital of Northern Theatre CommandShenyangLiaoningChina Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Neurology Central Hospital Affiliated to Shenyang Medical CollegeShenyangChina Neurology The People’s Hospital of Anyang CityAnyangChina Neurology Haicheng Hospital of Traditional Chinese MedicineHaichengChina Neurology Liaocheng People's HospitalLiaochengShandongChina Neurology The First Affiliated Hospital of Jiamusi UniversityJiamusiChina Neurology Zhengzhou Central HospitalZhengzhouChina Neurology The 32183 Military Hospital of PLABaichengChina Neurology The Second Affiliated Hospital of Harbin Medical UniversityHarbinChina Neurology Tieling County Central HospitalTielingChina Clinical Sciences Liverpool School of Tropical MedicineLiverpoolUK Neurology Peking Union Medical College HospitalBeijingChina The George Institute for Global Health SydneyNew South WalesAustralia 不详

Background Intravenous recombinant tissue plasminogen activator(r-tPA)and urokinase(UK)are both recommended for the treatment of acute ischaemic stroke(AIS)in China,but with few comparative outcome data being *** aimed to compare the outcomes of these two thrombolytic agents for the treatment of patients within 4.5 hours of onset of AIS in routine clinical practice in *** A pre-planned,prospective,nationwide,multicentre,real-world registry of consecutive patients with AIS(age≥18 years)who received r-tPA or UK within 4.5 hours of symptom onset according to local decision-making and guideline recommendations during *** primary effectiveness outcome was the proportion of patients with an excellent functional outcome(defined by modified Rankin scale scores 0 to 1)at 90 *** key safety endpoint was symptomatic intracranial haemorrhage according to standard *** logistic regression was used for comparative analysis,with adjustment according to propensity scores to ensure balance in baseline *** Overall,4130 patients with AIS were registered but 320 had incomplete or missing data,leaving 3810 with available data for analysis of whom 2666 received r-tPA(median dose 0.88(IQR 0.78-0.90)mg/kg)and 1144 received UK(1.71(1.43-2.00)×104 international unit per kilogram).There were several significant intergroup differences in patient characteristics:r-tPA patients were more educated,had less history of stroke,lower systolic blood pressure,greater neurological impairment and shorter treatment times from symptom onset than UK ***,in adjusted analysis,the frequency of excellent outcome(OR 1.18,95%CI 1.00 to 1.40,p=0.052)and symptomatic intracranial haemorrhage(OR 0.70,95%CI 0.33 to 1.47,p=0.344)were similar between *** UK may be as effective and carry a similar safety profile as r-tPA in treating mild to moderate AIS within guidelines in *** http://***

关键词： prospective routine nationwide

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Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)

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Agricultural Sciences 2015年第1期6卷 78-86页

作者： Yupeng cui Xuke Lu Delong Wang Junjuan Wang Zujun Yin Weili Fan Shuai Wang Wuwei Ye State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural Sciences Anyang China

Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-toleranc

关键词： Upland Cotton (Gossypium hirsutum L.) Salt Stress Differential Protein Mass Spectrum Analysis

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Pervasiveness of Predatory Spiders in Bt Cotton and Survivorship of Pardosa astrigera on Raised Live Insects

Pervasiveness of Predatory Spiders in Bt Cotton and Survivor...

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中国农学会棉花分会2018年年会

作者： Muhammad Nasir Zhang Shuai Luo Junyu Ma Chao Zhao Chenchen cui Jinjie Institute of Cotton Research (ICR) Chinese Academy of Agricultral Sciences (CAAS)

Spiders are one of the most universal group of predatory organisms belonging to animal *** regarding to identification of spiders prevailing in genetically modified crop(Bt-cotton) and rearing of Pardosa astrigera(wolf spider),on live insects,given as diet under laboratory conditions,were carried out at Institute of Cotton Research(ICR) of CAAS,anyang,Henan.A number of spiders were collected during September-October,2017,using beat bucket method and vial tapping/jarring from 20 points,each with 1 m,5 in one acre(about 4 047 m),4 corners sides(5 meter inside the crop) and one central point of each acre) following x-manner within Bt cotton covering an area of 4 acres,East farms,ICR,*** of each collected spider was carried out,based on morphometric characteristics of various body parts according to following the information and different scientific reports from China and World Spiders catalog *** regarding survival of wolf spider under laboratory conditions were conducted for 8 *** various reared insects,Drosophila melanogaster,fruit fly(FF);Myzus persicae,green peach aphid(GPA) and Musca domestica,house fly(HF) were given in diet separately and in a specific combination(1 time FF,2 time GPA and 3 time HF) on every second day along with water soaked cotton swab during the whole study period.A total number of 172 spiders were identified from the current collection with 10 different spider’s species(Oxyopes sertatus,Peucetia latikae,Hylyphantes graminicola,Pardosa astrigera,Steatoda triangulosa,Misumenops tricuspidatus,Xysticus ephippiatus,Neoscona theisi,Plexippus setipes and Cheiracanthium uncinatum) belong to 8 different families(Oxyopidae,Linyphiidae,Lycosidae,Theridiidae,Thomisidae,Araneidae,Salticidae and Eutichuridae).Percentage prevalence of each spider’s family was lingered between 1.74% and 22.09%.Maximum prevalence(22.09%) was of Oxyopidae(n=38) followed by 21.51% Linyphiidae(n=37),17.44% Lycosidae(n=30),15.17% Th

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澳大利亚健康与体育课程标准解读

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体育学刊 2018年第2期25卷 85-90页

作者：刁玉翠李梦欣党林秀董翠香华东师范大学体育与健康学院上海200241 安阳师范学院国际交流与教育学院河南安阳455000 福建师范大学体育科学学院福建福州350007

通过对澳大利亚2016版健康与体育课程标准文本的分析,发现以下特征:强调健康素养的培育,重视个人、社会及群体的健康促进;突出课程重点内容,重视课程内容体系的纵向衔接、横向贯通和均衡发展;强调成就标准与课程内容的协调衔接,增强评... 详细信息

通过对澳大利亚2016版健康与体育课程标准文本的分析,发现以下特征:强调健康素养的培育,重视个人、社会及群体的健康促进;突出课程重点内容,重视课程内容体系的纵向衔接、横向贯通和均衡发展;强调成就标准与课程内容的协调衔接,增强评价的可操作性;重视通用素养与跨课程重点领域的渗透,促进学生多种能力的培养;重视教育公平,关注学生差异性及其多样化的学习需求。对我国义务教育体育与健康课程改革的启示为:协调学生发展核心素养与体育学科核心素养的关系,注重推动学生发展核心素养在体育与健康学科中的落实;采取优势取向方式,促进每个学生的发展;凸显课程重点内容,优化课程内容结构体系;完善学业成就标准,增强评价可操作性。

关键词：学校体育健康与体育课程标准通用能力核心素养澳大利亚

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黄河流域田间棉铃虫对转Bt基因棉抗性监测

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棉花学报 2010年第4期22卷 297-303页

作者：张洋张帅崔金杰中国农业科学院棉花研究所河南安阳455000

利用改进的单雌系F1/F2代法,对黄河流域主要棉区河南安阳县、河北威县、山东武城县的棉铃虫种群进行了系统监测。在2007-2009年,连续3年保守地估计了以上3个主要植棉县棉铃虫种群对Cry1Ac的抗性基因频率。结果表明,山东武城棉铃虫种群3... 详细信息

利用改进的单雌系F1/F2代法,对黄河流域主要棉区河南安阳县、河北威县、山东武城县的棉铃虫种群进行了系统监测。在2007-2009年,连续3年保守地估计了以上3个主要植棉县棉铃虫种群对Cry1Ac的抗性基因频率。结果表明,山东武城棉铃虫种群3年的抗性等位基因频率分别为0.00086、0.00097和0.00052;安阳田间棉铃虫种群2008和2009年抗性等位基因频率分别为0.00090和0.00103;河北威县的田间棉铃虫种群抗性等位基因频率为0。所有家系F1代和F2代的相对平均发育级别均值比较结果显示,山东武城县棉铃虫种群耐受性高于其它2个地区,其次为安阳县种群,威县种群敏感度最高。总之,我国黄河流域这3个主要棉区田间棉铃虫种群对Cry1Ac毒素还没有产生明显的抗性,抗性基因频率仍处于正常水平,但是棉铃虫对Cry1Ac毒素的耐受性有升高趋势,需要进一步加强全国性的监测工作。

关键词：单雌系F1/F2代法抗性基因频率相对平均发育级别棉铃虫

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论自由全面发展是马克思主义人学的终极关怀

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安阳师范学院学报 2006年第4期 1-3页

作者：崔赟河南大学河南开封475001

马克思主义人学将社会实践视为社会历史发展和社会形态演进的内在动力,将人的全面自由自觉的发展视为社会发展的理论支点、内在要求、必然趋势与最高目的。这表明,马克思主义人学具有强烈的实践性,唯有将物质生产实践及其在此基础上形... 详细信息

马克思主义人学将社会实践视为社会历史发展和社会形态演进的内在动力,将人的全面自由自觉的发展视为社会发展的理论支点、内在要求、必然趋势与最高目的。这表明,马克思主义人学具有强烈的实践性,唯有将物质生产实践及其在此基础上形成的各种社会关系作为考察人的生存、人的本质、人的价值学问题的支点,才能为当代中国人学体系的建构与发展提供正确理论视界。

关键词：自由全面发展马克思主义人学终极关怀

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