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Novel blood-based microRNA biomarker panel for early diagnosis of pancreatic cancer

World Journal of Gastrointestinal Oncology 2014年第1期6卷 22-33页

作者： Ganepola AP Ganepola John R Rutledge Paritosh Suman anusak yiengpruksawan David H Chang Center for Cancer Research and Genomic Medicine The Valley Hospital Department of Surgery The Valley Hospital

AIM:To develop a panel of blood-based diagnostic biomarkers consisting of circulating microRNAs for the detection of pancreatic cancer at an early ***:Blood-based circulating microRNAs were profiled by high throughput screening using microarray analysis,comparing differential expression between early stage pancreatic cancer patients(n = 8) and healthy controls(n = 11).A panel of candidate microRNAs was generated based on the microarray signature profiling,including unsupervised clustering and statistical analysis of differential expression levels,and findings from the published *** selected candidate microRNAs were then confirmed using TaqMan real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR) to further narrow down to a three-microRNA diagnostic *** three-microRNA diagnostic panel was validated with independent experimental proce-dures and instrumentation of RT-qPCR at an independent venue with a new cohort of cancer patients(n = 11),healthy controls(n = 11),and a group of high risk controls(n = 11).Receiver operating characteristic curve analysis was performed to assess the diagnostic capability of the three-microRNA ***:In the initial high throughput screening,1220 known human microRNAs were screened for differential expression in pancreatic cancer patients versus controls.A subset of 42 microRNAs was then generated based on this data analysis and current published *** microRNAs were selected from the list of 42 targets for confirmation study,and three-microRNAs,miR-642b,miR-885-5p,and miR-22,were confirmed to show consistent expression between microarray and *** three microRNAs were then validated and evaluated as a diagnostic panel with a new cohort of patients and controls and found to yield high sensitivity(91%) and specificity(91%) with an area under the curve of 0.97(P < 0.001).Compared to the CA19-9 marker at 73%,the three-microRNA panel has higher sensitivity although

关键词： MicroRNA Diagnosis Biomarkers Pancreatic cancer Blood plasma Circulating

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Advancing robotic surgery skills with a simple novel model for robotic training

Advancing robotic surgery skills with a simple novel model f...

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2015年浙江省外科学学术年会暨国家级肝胆胰疾病诊治进展学习班

作者： Wang Zhifei Liu Junwei Zhang Yuhua Hong Defei Yu Xiaofen Zhang Dahong anusak yiengpruksawan Huang Dongsheng Department of Hepatobiliary and Pancreatic Surgery Zhejiang People's Provincial Hospital

Background Both dry lab and wet lab training are importance for advanced surgery *** assisted surgery,though lowering the technical demand for some key surgery skills such as suturing,tying knots compared with traditional laparoscopic surgery,to master the techniques for the key surgery procedures is still an challenging *** challenge lies in several issues at the beginning for robotic *** coordination with

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机器人辅助腹腔镜胰十二指肠切除术

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中华消化外科杂志 2009年第4期8卷 315-317页

作者：王知非 anusak yiengpruksawan Nino Carnevale 周宁新哈尔滨医科大学附属第四医院普通外科 150001 美国新泽西州谷医院肿瘤外科及机器人微创外科 NJ 07450-2736 北京二炮总医院普通外科、肝胆胃肠研究所 100088

胰十二指肠切除术（pancreaticoduodenectomy，PD）被认为是普通外科手术中较难和复杂的手术，即使对于有经验的外科医生也是如此。腹腔镜胰十二指肠切除术（laparoscopic pancreaticoduodenectomy，LPD）由于手术时间长，腹腔镜器械局... 详细信息

胰十二指肠切除术（pancreaticoduodenectomy，PD）被认为是普通外科手术中较难和复杂的手术，即使对于有经验的外科医生也是如此。腹腔镜胰十二指肠切除术（laparoscopic pancreaticoduodenectomy，LPD）由于手术时间长，腹腔镜器械局限性等造成技术上的困难，使很多医生对开展LPD望而却步。LPD本身也一直存在争议，因此LPD一直被认为是微创外科中最具有挑战性的手术。

关键词：胰十二指肠切除术达芬奇机器人外科外科技术

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International consensus statement on robotic hepatectomy surgery in 2018

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World Journal of Gastroenterology 2019年第12期25卷 1432-1444页

作者： Rong Liu Go Wakabayashi Hong-Jin Kim Gi-Hong Choi anusak yiengpruksawan Yuman Fong Jin He Ugo Boggi Roberto I Troisi Mikhail Efanov Daniel Azoulay Fabrizio Panaro Patrick Pessaux Xiao-Ying Wang Ji-Ye Zhu Shao-Geng Zhang Chuan-Dong Sun Zheng Wu Kai-Shan Tao Ke-Hu Yang Jia Fan Xiao-Ping Chen Second Department of Hepatopancreatobiliary Surgery Chinese People’s Liberation Army (PLA) General Hospital Center for Advanced Treatment of Hepatobiliary and Pancreatic Diseases Ageo Central General Hospital Department of Surgery Yeungnam University Hospital Division of Hepatobiliary Pancreatic Surgery Department of Surgery Yonsei University College of Medicine Minimally Invasive Surgery Division Department of SurgeryFaculty of Medicine Siriraj Hospital Mahidol University Department of Surgery City of Hope Medical Center Department of Surgery the Johns Hopkins Hospital Division of General and Transplant Surgery Pisa University Hospital Department of Clinical Medicine and Surgery Federico II University Department of Hepato-Pancreato-Biliary Surgery Moscow Clinical Scientific Center Hepato-Biliary Center Paul Brousse University Hospital Hepato-Biliary Center Tel Hashomer University Hospital Department of Surgery/Division of HBP Surgery and Transplantation Montpellier University Hospital-School of Medicine Head of the Hepato-biliary and pancreatic surgical unit Nouvel Hopital Civil Department of Liver Surgery and Transplantation Liver Cancer Institute Zhongshan Hospital Fudan University Department of Hepatobiliary Surgery Peking University People’s Hospital Department of Hepatobiliary Surgery 302 Hospital of Chinese PLA Department of Hepatobiliary and Pancreatic Surgery the Affiliated Hospital of Qingdao University Department of Hepatobiliary Surgery the First Affiliated Hospital of Xi’an Jiaotong University Department of Hepatobiliary Surgery Xijing Hospital the Fourth Military Medical University Evidence Based Medicine Center School of Basic Medical Sciences Lanzhou University Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

The robotic surgical system has been applied in liver surgery. However,controversies concerns exist regarding a variety of factors including the safety,feasibility, efficacy, and cost-effectiveness of robotic surgery. To promote the development of robotic hepatectomy, this study aimed to evaluate the current status of robotic hepatectomy and provide sixty experts' consensus and recommendations to promote its development. Based on the World Health Organization Handbook for Guideline Development, a Consensus Steering Group and a Consensus Development Group were established to determine the topics, prepare evidence-based documents, and generate recommendations. The GRADE Grid method and Delphi vote were used to formulate the recommendations. A total of 22 topics were prepared analyzed and widely discussed during the 4 meetings. Based on the published articles and expert panel opinion, 7 recommendations were generated by the GRADE method using an evidence-based method, which focused on the safety, feasibility, indication,techniques and cost-effectiveness of hepatectomy. Given that the current evidences were low to very low as evaluated by the GRADE method, further randomized-controlled trials are needed in the future to validate these recommendations.

关键词： Minimally invasive surgery Robotic hepatectomy Laparoscopic hepatectomy Hepatectomy resection Consensus statement

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达芬奇机器人手术系统在消化外科中的应用

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中华消化外科杂志 2010年第6期9卷 478-480页

作者：王知非周宁新刘全达 anusak yiengpruksawan 许军哈尔滨医科大学附属第四医院普通外科 150001 北京二炮总医院普通外科、肝胆胃肠研究所 100088 美国新泽西州谷医院肿瘤外科及机器人微创外科 NJ07450-2736

机器人外科的产生是远程外科、军事外科以及微创外科三方面结合的直接结果。在越来越多的腹部外科国际会议上，机器人外科已经占据一席之地。美国胃肠和内镜外科医师学会微创机器人协会于2007年12月颁布了机器人外科的共识。

关键词：机器人手术系统消化外科临床应用微创外科

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International consensus statement on robotic pancreatic surgery

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Hepatobiliary Surgery and Nutrition 2019年第4期8卷 345-360页

作者： Rong Liu Go Wakabayashi Chinnusamy Palanivelu Allan Tsung Kehu Yang Brian KPGoh Charing Ching-Ning Chong Chang Moo Kang Chenghong Peng Eli Kakiashvili Ho-Seong Han Hong-Jin Kim Jin He Jae Hoon Lee Kyoichi Takaori Marco Vito Marino Shen-Nien Wang Tiankang Guo Thilo Hackert Ting-Shuo Huang yiengpruksawan anusak Yuman Fong Yuichi Nagakawa Yi-Ming Shyr Yao-Ming Wu Yupei Zhao Department of Hepatopancreatobiliary Surgical Oncology Chinese People’s Liberation Army(PLA)General HospitalBeijing 100853China Center for Advanced Treatment of Hepatobiliary and Pancreatic Diseases Ageo Central General HospitalAgeoJapan Division of Gastrointestinal Surgery and Minimal Access Surgery GEM Hospital and Research CentreCoimbatoreIndia Division of Surgical Oncology Gastrointestinal Disease Specific Research GroupThe Ohio State University Wexner Medical Center Department of SurgeryColumbusOHUSA Evidence-Based Medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhou 730000China Department of Hepatopancreatobiliary and Transplant Surgery Singapore General HospitalSingapore Department of Surgery Division of Hepatobiliary and Pancreatic SurgeryPrince of Wales Hospitalthe Chinese University of Hong KongHong KongChina Division of HBP Surgery Yonsei University College of MedicinePancreatobiliary Cancer CenterYonsei Cancer CenterSeverance HospitalSeoulKorea Pancreatic Disease Centre Ruijin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of General Surgery Galilee Medical CenterNahariyaIsrael Department of Surgery Seoul National University College of MedicineSeoul National University Bundang HospitalSeoulKorea Department of Surgery Yeungnam University HospitalDaeguKorea Department of Surgery The Johns Hopkins HospitalBaltimoreMDUSA Division of Hepatobiliary&Pancreas Surgery Department of SurgeryAsan Medical CenterSeoulKorea Department of Surgery Kyoto University HospitalShogoinSakyo-KuKyotoJapan Department of General Surgery Azienda OspedalieraOspedali Riuniti Villa Sofia-CervelloPalermoItaly Division of General and Digestive Surgery Department of SurgeryKaohsiung Medical University HospitalKaohsiung Department of General Surgery Gansu Provincial HospitalLanzhou 730030China Department of General Visceral and Transplantation SurgeryHeidelberg University HospitalHeidelbergGermany Division of General Surge

The robotic surgical system has been applied to various types of pancreatic surgery. However, controversies exist regarding a variety of factors including the safety, feasibility, efficacy, and cost-effectiveness of robotic surgery. This study aimed to evaluate the current status of robotic pancreatic surgery and put forth experts' consensus and recommendations to promote its development. Based on the WHO Handbook for Guideline Development, a Consensus Steering Group* and a Consensus Development Group were established to determine the topics, prepare evidence-based documents, and generate recommendations. The GRADE Grid method and Delphi vote were used to formulate the recommendations. A total of 19 topics were analyzed. The first 16 recommendations were generated by GRADE using an evidence-based method (EBM) and focused on the safety, feasibility, indication, techniques, certification of the robotic surgeon, and cost-effectiveness of robotic pancreatic surgery. The remaining three recommendations were based on literature review and expert panel opinion due to insufficient EBM results. Since the current amount of;evidence was low/meager as evaluated by the GRADE method, further randomized controlled trials (RCTs) are needed in the future to validate these recommendations.

关键词： Robotic surgery consensus statement pancreatectomy pancreaticoduodenectomy pancreatic enucleation

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Successful treatment of esophageal metastasis from hepatocellular carcinoma using the da Vinci robotic surgical system

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World Journal of Gastrointestinal Surgery 2011年第6期3卷 82-85页

作者： Wiroon Boonnuch Thawatchai Akaraviputh Carnivale Nino anusak yiengpruksawan Arthur Andrew Christiano Minimally Invasive Surgery Center Division of General SurgeryDepartment of SurgeryFaculty of Medicine Siriraj HospitalMahidol UniversityBangkok10700Thailand Department of Surgery the Valley HospitalValley Health SystemNJ 07652United States Department of Pathology the Valley HospitalValley Health SystemNJ 07652United States

A 59-year-old man with metastatic an esophageal tumor from hepatocellular carcinoma(HCC) presented with progressive *** had undergone liver transplantation for HCC three and a half years *** presentation,his radiological and endoscopic examinations suggested a submucosal tumor in the lower esophagus,causing a luminal *** performed complete resection of the esophageal metastases and esophagogastrostomy reconstruction using the da Vinci robotic *** was uneventful and he was been doing well 2 mo after surgery.α-fetoprotein level decreased from 510 ng/mL to 30 ng/mL *** the follow-up period,he developed a recurrent esophageal stricture at the anastomosis site and this was successfully treated by endoscopic esophageal dilatation.

关键词： Hepatocellular carcinoma Esophageal metastasis da Vinci robotic surgery

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