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Development of otology specific outcome measure:Ear Outcome Survey-16 (EOS-16)

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Journal of Otology 2021年第3期16卷 150-157页

作者： Juha T.Laakso Juha Silvola Timo Hirvonen Samuli Suutarla Ilkka Kivekas Riitta Saarinen Lotta Haavisto Jaakko Laitakari antti a.aarnisalo Aarno Dietz Jussi Jero Maija Hytonen Saku T.Sink Department of Otorhinolaryngology-Head and Neck Surgery Head and Neck CenterHelsinki University Hospital and University of HelsinkiHelsinkiFinland Department of Ear Noseand Throat-Division of SurgeryAkershus University HospitalLørenskogNorway Department of Otorhinolaryngology Kymenlaakso Central HospitalKotkaFinland Department of Otorhinolaryngology-Head and Neck Surgery Tampere University Hospital and Tampere UniversityTampereFinland Department of Otorhinolaryngology-Head and Neck Surgery Turku University Hospital and University of TurkuTurkuFinland Department of Otorhinolaryngology-Head and Neck Surgery Oulu University Hospital and PEDEGO Research UnitUniversity of Oulu and Medical Research Center OuluFinland Department of Otorhinolaryngology-Head and Neck Surgery Kuopio University Hospital and University of KuopioKuopioFinland

Purpose An important outcome measure of patient care is the impact on the patient’s health-related quality of life(HRQoL).Current ear-specific HRQoL instruments are designed for one diagnosis and emphasize different subdivisions such as symptoms,hearing problems,psychosocial impact,and the need for *** optimal length of the recall period has not been *** these reasons,a new survey is needed that would cover most chronic ear *** A preliminary 24-item survey(EOS-24)was *** adult patients(included n=186)with one of seven different chronic otologic conditions from all university hospitals in Finland were recruited to respond to EOS-24 and the 15D general HRQoL *** recruiting otologists evaluated the severity of the disease and the disability caused by it.A control group was *** on the patients’responses in different diagnosis groups,the items were reduced according to pre-defined *** resulting survey was validated using a thorough statistical *** The relevance and necessity of the original 24 items were thoroughly investigated,leading to the exclusion of 8 items and the modification of *** remaining 16 items were well-balanced between subdivisions and were useful in all seven diagnosis groups,thus constituting the final instrument,*** most suitable recall period was three *** EOS-16 has been created according to the HRQoL survey guidelines with a versatile nationwide patient *** survey has been validated and can be used for a wide range of chronic ear diseases as a HRQoL instrument.

关键词： Otology Chronic ear diseases HRQoL Health-related quality of life PROM

Label-free visualization of cholesteatoma in the mastoid and tympanic membrane using CARS microscopy

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Journal of Otology 2016年第3期11卷 127-133页

作者： Jing Zou antti Isomaki Timo Hirvonen antti aarnisalo Jussi Jero Ilmari Pyykko Department of Otolaryngology-Head and Neck Surgery Center for Otolaryngology-Head&Neck Surgery of Chinese PLAChanghai HospitalSecond Military Medical University Hearing and Balance Research Unit Field of Oto-laryngologySchool of MedicineUniversity of Tampere Biomedicum Imaging Unit Faculty of MedicineUniversity of Helsinki Department of Otorhinolaryngology-Head and Neck Surgery Helsinki University Central Hospital

Objective:The present study aimed to evaluate the possibility of using coherent anti-Stokes Raman spectroscopy（CARS） microscopy to determine the specific molecular morphology of cholesteatoma by detecting the natural vibrational contrast of the chemical bonds without any *** and methods:Specimens from the mastoid and tympanic membrane with and without cholesteatoma were analyzed using CARS microscopy,two-photon excited fluorescence（TPEF） microscopy,and the second harmonic generation（SHG） ***:In cholesteatoma tissues from the mastoid,a strong resonant signal at 2845 cm;was observed by CARS,which indicated the detection of the CH;hydro-carbon lipid bonds that do not generate visible signals at 2940 cm;suggestive of CH;bonds in amino acids.A strong resonant signal at 2940 cm;appeared in an area of the same specimen,which also generated abundant signals by TPEF and SHG microscopy at 817 nm,which was suggestive of *** the tympanic membrane specimen with cholesteatoma,a strong resonant signal with corrugated morphology was detected,which indicated the presence of lipids.A strong signal was detected in the tympanic membrane with chronic otitis media using TPEF/SHG at 817 nm,which indicated collagen *** CARS and TPEF/SHG images were in accordance with the histology ***:These results suggest the need to develop a novel CARS microendoscope that can be used in combination with TPEF/SHG to distinguish cholesteatoma from inflammatory tissues.

关键词： Cholesteatoma Imaging Label-free Raman spectroscopy High resolution

Effects of p27^(Kip1)- and p53- shRNAs on kanamycin damaged mouse cochlea

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World Journal of Otorhinolaryngology 2012年第1期2卷 1-7页

作者： Laura Pietola Jussi Jero Reetta Jalkanen Teemu J Kinnari Olga Jero Mikko Frilander Katri Pajusola Marjo Salminen antti a.aarnisalo Department of Otorhinolaryngology University of Helsinki Folkh?lsan Institute of Genetics Biomedicum Helsinki 00014 Helsinki Finland Institute of Biotechnology University of Helsinki Molecular Cancer Biology Program Biomedicum Helsinki 00014 Helsinki Finland Department of Basic Veterinary Science Division of Biochemistry University of Helsinki

AIM： To study the effects of adeno-associated virus （AAV） delivered short hairpin RNAs （shRNAs） on adult CD-1 mouse cochlea damaged by aminoglycoside anti-biotic kanamycin. METHODS： Three different shRNAs were designed （p27^Kip1, p53 and p27^Kip1＋p53） and tested in COS cells. A total of 20 adult CD-1 mice were used in the experiment. Mice were divided into fve different groups （four animals/group） depending on the AAV-shRNA construct they received and whether they received kanamycin or not. Saline and AAV-EGFP injected animals were used as controls. All constructs were injected through the round window membrane （RWM） into the cochlea. Cochleae were harvested after 1 mo. Apoptosis was detected with Tunel labeling from paraffin-embedded cochlear tissue ***： AAV2/2-p27^Kip1-shRNA and AAV2/2-p53-shRNA were tested in COS cells. Western blotting analysis confirmed that both constructs silenced their target genes effectively in the cell culture. AAV2/2-shRNA constructs were injected into the cochlea of CD-1 mice through the intact RWM. Cotransductionof Cotransduction of individual AAV2/2-shRNAs with AAV2/2-EGFP resulted in EGFP expression in the organ of *** Kanamycin treatment had no effect on the expression pattern of the EGFP. AAV2/2-shRNA treated mice （either with p53 or p27Kip1and p53 together） showed fewer apop-totic hair cells in the cochlea than the control group （P 〈 0.05; AAV2/2-p53-shRNA vs saline P = 0.00014; AAV2/2-p27＋p53-shRNA vs saline P = 0.0011）. AAV2/2-p27-shRNA injected cochleae showed no significant difference in the number of apoptotic cells when compared to the saline injected ***： Silencing of p53 protein in the kana-mycin treated ears may decrease cell death in the organ of Corti.

关键词： Inner ear Adeno-associated virus Short hairpin RNA p27 p53 Kanamycin Apoptosis

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芬兰人工耳蜗植入术加强无菌措施的方法

中华耳科学杂志 2018年第1期16卷 118-121页

作者：邹静秦晶姜晓瑜李勇周蓉珏徐菲 Timo Hirvonen antti aarnisalo Jussi Jero 郑宏良中国人民解放军第二军医大学长海医院耳鼻咽喉头颈外科全军耳鼻咽喉头颈外科中心上海中国人民解放军第二军医大学长海医院麻醉学部手术室上海 Department of Otorhinolaryngology-Head and Neck Surgery University of Helsinkiand Helsinki University HospitalHelsinkiFinland

人工耳蜗植入已经在全球广泛开展并帮助广大聋病患者重新返回正常生活,且并发症的发生率也越来越少;然而,围手术期和术后晚期感染性并发症仍然时有发生。在某些特殊病例中,当抗生素无效时,植入体也将会被废弃,重新植入将会显著增加患者... 详细信息

人工耳蜗植入已经在全球广泛开展并帮助广大聋病患者重新返回正常生活,且并发症的发生率也越来越少;然而,围手术期和术后晚期感染性并发症仍然时有发生。在某些特殊病例中,当抗生素无效时,植入体也将会被废弃,重新植入将会显著增加患者经济负担。芬兰的所有人工耳蜗植入中心都采用一种术中加强无菌措施以便预防或者减少感染性并发症的发生。其关键点是,从手术室的术前准备到完成植入手术,全程严格实施无菌操作,在完成颞骨钻磨工作以后,清理术野,医生和护士重新进行手部消毒,更新术区的无菌巾和医护人员的手套,打开人工耳蜗植入体的包装,固定植入体并安装耳蜗电极,完成后续程序。在仅预防性使用抗生素的情况下,610次人工耳蜗植入未发生植入体的原发性感染并发症。邹静于2016年在赫尔辛基大学中心医院耳鼻咽喉头颈外科学习人工耳蜗植入的临床相关技术,将这一加强无菌措施在长海医院推广应用。除了因为医院的实际情况而存在一些较小的差异外,关键步骤完全可以在长海医院实施。本文将对该技术进行详细报道,期望今后在中国全国推广这一效价比很高的技术。

关键词：人工耳蜗植入无菌措施感染并发症手术

患急性乳突炎和偶发性乳突T2WI高信号的儿童与成人病例的MRI的特征比较

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国际医学放射学杂志 2016年第5期 593-594页

作者： R.Saat G.Mahmood A.Laulajainen-Hongisto L.Lempinen A.a.aarnisalo J.Jero 高国栋 Department of Radiology HUS Medical Imaging CentreHelsinki University Hospital and University of Helsinki

摘要目的本研究通过比较偶发性乳突T2WI高信号病人与临床确诊为急性乳突炎病人的MR影像表现,明确两者间特征性差异。方法对35例临床确诊急性乳突炎的成年及儿童病人,以及34例连续、偶然发现乳突50%以上T2高信号匹配的对照组病例,回顾性... 详细信息

摘要目的本研究通过比较偶发性乳突T2WI高信号病人与临床确诊为急性乳突炎病人的MR影像表现,明确两者间特征性差异。方法对35例临床确诊急性乳突炎的成年及儿童病人,以及34例连续、偶然发现乳突50%以上T2高信号匹配的对照组病例,回顾性分析两者乳突的信号,扩散及强

关键词：乳突病人乳头突颞骨急性乳突炎 T2WI 儿童偶发性病例 MRI