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Cellular & Molecular Immunology 2018年第4期15卷 353-366页

作者： Laura Gómez-Jaramillo Raquel Romero-garcía Gema Jiménez-Gómez Lisa Riegle Ana Belén Ramos-Amaya Joséantonio Brieva Marie Kelly-Worden antonio Campos-Caro Unidad de Investigación Hospital Universitario Puerta del MarCádizSpain Department of Biology Ball State UniversityMuncieINUSA

The production and secretion of antibodies by human plasma cells(PCs)are two essential processes of humoral *** secretion process relies on a group of proteins known as soluble N-ethylmaleimide-sensitive factor attachment protein receptors(SNAREs),which are located in the plasma membrane(t-SNAREs)and in the antibody-carrying vesicle membrane(v-SNARE),and mediate the fusion of both *** have previously shown that SNAP23 and STX4 are the t-SNAREs responsible for antibody ***,using human PCs and antibody-secreting cell lines,we studied and characterized the expression and subcellular distribution of vesicle associated membrane protein(VAMP)isoforms,demonstrating that all isoforms(with the exception of VAMP1)are expressed by the referenced ***,the functional role in antibody secretion of each expressed VAMP isoform was tested using *** results show that VAMP2 may be the v-SNARE involved in vesicular antibody *** further support this conclusion,we used tetanus toxin light chain to cleave VAMP2,conducted experiments to verify co-localization of VAMP2 in antibody-carrying vesicles,and demonstrated the coimmunoprecipitation of VAMP2 with STX4 and SNAP23 and the in situ interaction of VAMP2 with *** together,these findings implicate VAMP2 as the main VAMP isoform functionally involved in antibody secretion.

关键词： exocytosis human immunoglobulin plasma cells VAMP proteins

Preparation and Characterization of Flexible, Transparent and Thermally Stable Aromatic Co-polyamides

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Chinese Journal of Polymer Science 2019年第2期37卷 136-141页

作者： José antonio Tec-Sánchez Andrés Iván Oliva Arias Manuel Aguilar-Vega Juan Valerio Cauich-Rodríguez José Luis Santiago-garcía Unidad de Materiales Centro de Investigación Científica de Yucatán A. C. Centro de Investigación y de Estudios Avanzados del IPN Unidad Mérida Departamento de Física Aplicada

Two aromatic co-polyamides were synthesized combining two diacid monomers containing bulky pendant groups, 5-(9,10-dihydro-9,10-ethanoanthracene-11,12-dicarboximido)isophthalic acid(DEAIA) and 5-tert-butylisophthalic acid(TERT), with 4,4′-(hexafluoroisopropylidene)dianiline(HFA) or 2,3,5,6-tetramethyl-1,4-phenylenediamine(Durene) by direct polycondensation. The structures of the obtained aromatic co-polyamides were confirmed by FTIR, Raman and 1 H-NMR. The co-copolyamide films, DHTH and DDTD, exhibited rms-roughness values between 0.94 and 1.60 nm, respectively. Moreover, they presented good thermal stability up to300 °C. Young's moduli of the co-polyamide films were between 4.1 and 4.3 GPa. X-ray diffraction results showed that the co-polyamide films were amorphous due to the incorporation of both bulky pendant groups, tert-butyl and dibenzobarrelene. The combination of bulky pendant groups provided intrinsically transparent co-polyamide films with a transmittance higher than 88% in the range of 400-780 *** to these outstanding film and optical properties, they are suggested to be flexible substrates in applications for solar cell and other portable electronic devices.

关键词： Co-polyamides Flexible films Transmittance Thermal stability Polycondensation

The biosynthesis and wall-binding of hemicelluloses in cellulose-deficient maize cells:An example of metabolic plasticity

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Journal of Integrative Plant Biology 2015年第4期57卷 373-387页

作者： María de Castro Janice G.Miller José Luis Acebes antonio Encina Penélope garcía-Angulo Stephen C.Fry The Edinburgh Cell Wall Group Institute of Molecular Plant SciencesSchool of Biological SciencesDaniel Rutherford BuildingThe King's Buildings Laboratory of Plant Physiology Faculty of Biological and Environmental SciencesUniversity of Léon

Cell-suspension cultures（Zea mays L.,Black Mexican sweet corn） habituated to 2,6-dichlorobenzonitrile（DCB） survive with reduced cellulose owing to hemicellulose network modification. We aimed to de fine the hemicellulose metabolism modifications in DCB-habituated maize cells showing a mild reduction in cellulose at different stages in the culture cycle. Using pulse-chase radiolabeling, we fed habituated and non-habituated cultures with [3H]arabinose,and traced the distribution of 3H-pentose residues between xylans, xyloglucans and other polymers in several cellular compartments for 5 h. Habituated cells were slower taking up exogenous [3H]arabinose. Tritium was incorporated into polysaccharide-bound arabinose and xylose residues, but habituated cells diverted a higher proportion of their new [3H]xylose residues into（hetero） xylans at the expense of xyloglucan synthesis. During logarithmic growth, habituated cells showed slower vesicular traf ficking of polymers,especially xylans. Moreover, habituated cells showed a decrease in the strong wall-binding of all pentose-containing polysaccharides studied; correspondingly, especially in log phase cultures, habituation increased the proportion of 3H-hemicelluloses（[3H]xylans and [3H]xyloglucan） sloughed into the medium. These findings could be related to the cel walls’ cellulose-deficiency, and consequent reduction in binding sites for hemicelluloses; the data could also re fl ect the habituated cells’ reduced capacity to integrate arabinox ylans by extra-protoplasmic phenolic cross-linking, as well as xyloglucans, during wall assembly.

关键词： Arabinoxylan cell wall cross-linking 2,6-dichlorobenzonitrile Hemicellulose biosynthesis xyloglucan

Analysis of Formation in Communicative Competences for the Medical Act in Universidad de Antioquia Medicine School Undergraduate Program

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Journal of Biosciences and Medicines 2016年第12期4卷 76-88页

作者： José antonio garcía Pereáñez American University Corporation Medellin Colombia

This research study was conducted at the undergraduate program of the Medicine Faculty of Universidad de Antioquia. Its main purpose was to analyze the training in communication skills for the medical act. To this purpose, a triangulation between a representative survey of the students, some interviews and focal groups between students and professors, and an analysis of a curricular document on the contents of communication as a generic skill were done. This study allowed us to conclude that the definition of generic competence in communication cannot be established without the participation of the medicine curriculum actors, and that it is paramount to integrate this learning to this university program. The result of the present study is a proposal to include the communicative competence for the medical act in the curriculum of the Medicine Faculty of Universidad de Antioquia.

关键词： Communication Skill Medical Act Medical Education Generic Competence

Neuroprotective effect of immunomodulatory peptides in rats with traumatic spinal cord injury

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Neural Regeneration Research 2021年第7期16卷 1273-1280页

作者： Dulce Parra-Villamar Liliana Blancas-Espinoza Elisa garcia-Vences Juan Herrera-garcía Adrian Flores-Romero Alberto Toscano-Zapien Jonathan Vilchis Villa Rodríguez Barrera-Roxana Soria Zavala Karla antonio Ibarra Raúl Silva-garcía Unidad de Investigación Médica en Inmunología Hospital de PediatríaCentro Médico Nacional Siglo XXI/Instituto Mexicano del Seguro Social/Ciudad de MéxicoMéxico Centro de Investigación en Ciencias de la Salud(CICSA) Facultad de Ciencias de la SaludUniversidad Anáhuac MéxicoCampus NorteHuixquilucanEdo de MéxicoMéxico Centro de Investigación del Proyecto Camina A.C Ciudad de MéxicoMéxico

Several therapies have shown obvious effects on structural conservation contributing to motor functional recovery after spinal cord injury(SCI).Nevertheless, neither strategy has achieved a convincing *** purposed a combined therapy of immunomodulatory peptides that individually have shown significant effects on motor functional recovery in rats with *** objective of this study was to investigate the effects of the combined therapy of monocyte locomotion inhibitor factor(MLIF), A91 peptide, and glutathione monoethyl ester(GSH-MEE) on chronic-stage spinal cord *** Sprague-Dawley rats underwent a laminectomy of the T9 vertebra and a moderate *** groups were included: sham, PBS, MLIF + A91, MLIF + GSH-MEE, A91 + GSH-MEE, and MLIF + A91 + *** months after injury, motor functional recovery was evaluated using the open field *** and white matter preservation was evaluated using hematoxylin & eosin staining and Luxol Fast Blue staining, *** number of motoneurons in the ventral horn and the number of axonal fibers were determined using hematoxylin & eosin staining and immunohistochemistry, *** deposition was evaluated using Masson's trichrome *** combined therapy of MLIF, A91, and GSH-MEE greatly contributed to motor functional recovery and preservation of the medullary parenchyma, white matter, motoneurons, and axonal fibres, and reduced the deposition of collagen in the lesioned *** combined therapy of MLIF, A91, and GSH-MEE preserved spinal cord tissue integrity and promoted motor functional recovery of rats after *** study was approved by the National Commission for Scientific Research on Bioethics and Biosafety of the Instituto Mexicano del Seguro Social under registration number R-2015-785-116(approval date November 30, 2015) and R-2017-3603-33(approval date June 5, 2017).

关键词： glutathione monoethyl ester monocyte locomotion inhibitor factor motor functional recovery neuroprotection neurorestoration peptides protective autoimmunity spinal cord injury

Quasi-freestanding graphene on Ni（110）： A graphene/ metal contact with suppressed interface states

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Nano Research 2016年第6期9卷 1795-1800页

作者： antonio Politano Department of Physics University of Calabria via Ponte Bucci 3 I/C 1-87036 Rende (CS) Italy

Graphene/Ni（110） has been studied by time-resolved X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure. The C ls core level shows a splitting typical of periodically rippled interfaces. The analysis of the C K-edge reveals that the interface states previously observed for graphene/Ni（111） are suppressed in graphene/Ni（110）. This suppression is due to the reduced hybridization of the Dirac-cone electrons in graphene with the d-bands of the （110）-oriented nickel contacts. Our results show that, contrary to commensurate growth of graphene on Ni（111）, epitaxially grown graphene on Ni（110） behaves as a quasi-freestanding sheet, as the lattice mismatch gives rise to a moir6 reconstruction.

关键词： graphene electronic properties synchrotron radiation X-ray photoelectronspectroscopy near-edge X-rayphotoelectron spectroscopy

Phenolic metabolism and molecular mass distribution of polysaccharides in cellulose-deficient maize cells

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Journal of Integrative Plant Biology 2017年第7期59卷 475-495页

作者： María de Castro Romina Martínez-Rubio José L.Acebes antonio Encina Stephen C.Fry Penélope garcía-Angulo área de Fisiología Vegetal.Dpto.Ingenieríay Ciencias Agrarias.Facultad de Biologíay Ciencias Ambientales.Universidad de León The Edinburgh Cell Wall Group Institute of Molecular Plant Sciences School of Biological Sciences Daniel Rutherford BuildingThe King's Buildings

As a consequence of the habituation to low levels of dichlobenil （DCB）, cultured maize cells presented an altered hemicellulose cell fate with a lower proportion of strongly wall-bound hemicelluloses and an increase in soluble extracellular polymers released into the culture medium. The aim of this study was to investigate the relative molecular mass distributions of polysaccharides as well as phenolic metabolism in cells habituated to low levels of DCB （1.5 μM）. Generally, cell wall bound hemicelluloses and sloughed polymers from habituated cells were more homogeneously sized and had a lower weight-average relative molecular mass. In addition, polysaccharides underwent massive cross-linking after being secreted into the cell wall, but this cross-linking was less pronounced in habituated cells than in non-habituated ones. However, when relativized, ferulic acid and p-coumaric acid contents were higher in this habituated cell line. Feasibly, cells habituated to low levels of DCB synthesized molecules with a lower weight-average relative molecular mass, although cross-linked, as a part of their strategy to compensate for the lack of cellulose.

关键词： Phenolic metabolism and molecular mass distribution of polysaccharides in cellulose-deficient maize cells were for

Release of interleukin-10 and neurotrophic factors in the choroid plexus：possible inductors of neurogenesis following copolymer-1 immunization after cerebral ischemia

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Neural Regeneration Research 2018年第10期13卷 1743-1752页

作者： Yolanda Cruz Edna E.garcía Jessica V.Gálvez Stella V.Arias-Santiago Horacio G.Carvajal Raúl Silva-garcía Herlinda Bonilla-Jaime Julio Rojas-Castaneda antonio Ibarra Centro de Investigación en Ciencias de la Salud (CICSA) FCSUniversidad Anáhuac México Norte Huixquilucan Estado de México México Lab. De Biologia de la reproducción UAMI. Ciudad de México México Hospital de Pediatría CMN Siglo XXI. Ciudad de México México Subdirección de Medicina Experimental Instituto Nacional de PediatríaCiudad de México México Doctorado en Ciencias Biológicas División de Ciencias Biológicas y de la SaludUniversidad Autónoma MetropolitanaUnidad Iztapalapa.Ciudad de México México

Copolymer-1（Cop-1） is a peptide with immunomodulatory properties, approved by the Food and Drug Administration of United States in the treatment of multiple sclerosis. Cop-1 has been shown to exert neuroprotective effects and induce neurogenesis in cerebral ischemia models. Nevertheless, the mechanism involved in the neurogenic action of this compound remains unknown. The choroid plexus（CP） is a network of cells that constitute the interphase between the immune and central nervous systems, with the ability to mediate neurogenesis through the release of cytokines and growth factors. Therefore, the CP could play a role in Cop-1-induced neurogenesis. In order to determine the participation of the CP in the induction of neurogenesis after Cop-1 immunization, we evaluated the gene expression of various growth factors（brain-derived neurotrophic factor, insulin-like growth factor 1, neurotrophin-3） and cytokines（tumor necrosis factor alpha, interferon-gamma, interleukin-4（IL-4）, IL-10 and IL-17）, in the CP at 14 days after ischemia. Furthermore, we analyzed the correlation between the expression of these genes and neurogenesis. Our results showed that Cop-1 was capable of stimulating an upregulation in the expression of the genes encoding for brain-derived neurotrophic factor, insulin-like growth factor 1, neurotrophin-3 and IL-10 in the CP, which correlated with an increase in neurogenesis in the subventricular and subgranular zone. As well, we observed a downregulation of IL-17 gene expression. This study demonstrates the effect of Cop-1 on the expression of growth factors and IL-10 in the CP, in the same way, presents a possible mechanism involved in the neurogenic effect of Cop-1.

关键词： choroid plexus growth factors immunomodulation protective autoimmunity Cop-1 Copaxone stroke glatiramer acetate t MCAo focal cerebral ischemia

Secondary sclerosing cholangitis after critical COVID-19:Three case reports

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World Journal of Hepatology 2022年第8期14卷 1678-1686页

作者： Juan M Mayorquín-Aguilar Aldo Lara-Reyes Luis Alberto Revuelta-Rodríguez Nayelli C Flores-garcía Astrid ruiz-Margáin Marco antonio Jiménez-Ferreira Ricardo Ulises Macías-Rodríguez Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Pathology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico

BACKGROUND The global coronavirus disease 2019(COVID-19)pandemic has caused more than 5 million *** involvement is well described,including liver *** patients with critical COVID-19,a new entity called"post-COVID-19 cholangiopathy"has been *** SUMMARY Here,we present three patients with severe COVID-19 that subsequently developed persistent cholestasis and chronic liver *** three patients required intensive care unit admission,mechanical ventilation,vasopressor support,and broad spectrum antibiotics due to secondary *** transplant protocol was started for two of the three *** Severe COVID-19 infection should be considered a potential risk factor for chronic liver disease and liver transplantation.

关键词： SARS-CoV-2 Persistent cholestasis Liver chemistry Hypoxic cholangiopathy Case report

Prevention of bile spillage during cholecystectomy: current evidences and considerations

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Hepatobiliary Surgery and Nutrition 2020年第1期9卷 109-111页

作者： Maurizio Zizzo Lorenzo Manzini Magda Zanelli Loredana De Marco Carolina Castro ruiz antonio Manenti Valerio Annessi Surgical Oncology Unit Azienda UnitàSanitaria Locale-IRCCS di Reggio EmiliaArcispedale Santa Maria Nuova di Reggio EmiliaReggio EmiliaItaly Clinical and Experimental Medicine PhD Program University of Modena and Reggio EmiliaModenaItaly Pathology Unit Azienda UnitàSanitaria Locale-IRCCS di Reggio EmiliaArcispedale Santa Maria Nuova di Reggio EmiliaReggio EmiliaItaly General Surgery Unit Azienda UnitàSanitaria Locale-IRCCS di Reggio EmiliaOspedale Civile di GuastallaReggio EmiliaItaly Department of General Surgery Azienda Ospedaliero-Universitaria-PoliclinicoModenaItaly

We really appreciated Geramizadeh, " Bile spillage and incidental gall bladder adenocarcinoma ", and Sandstrom et al. ,"Bile spillage should be avoided in elective cholecystectomy " (1,2). Both papers have been recently published in HepatoBiliary Surgery and Nutrition, 2019 (1,2). Starting from an analysis of the recent paper by Horkoff et al. "Adverse outcomes after bile spillage in incidental gall bladder cancer " in Annals of Surgery April 2019, they introduced a comprehensive discussion about epidemiology, diagnosis, therapy, prognostic factors and outcomes of incidental gallbladder cancer (1-3). They analyzed the possible risks related to bile spillage after cholecystectomy, in particular (1,2).

关键词： Surgery cholecystectomy epidemiology diagnosis