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Production of reactive oxygen species and expression of inducible nitric oxide synthase in rat isolated Kupffer cells stimulated by Leptospira interrogans and Borrelia burgdorfen

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World Journal of Gastroenterology 2006年第19期12卷 3077-3081页

作者： antonella Marangoni Silvia Accardo Rita Aldini Massimo Guardigli Francesca Cavrini Vittorio Sambri Marco Montagnani Aldo Roda Roberto Cevenini Sezione di Microbiologia DMCSS University of Bologna BolognaItaly Istituto di Scienze Chimiche University of Bologna BolognaItaly Dipartimento di Scienze Farmaceutiche University of Bologna BolognaItaly Dipartimento di Medicina Interna e Gastroenterologia University of Bologna BolognaItaly

AIM： To evaluate the production of reactive oxygen species （ROS） and the expression of inducible nitric oxide synthase （iNOS） in rat isolated Kupffer cells （KCs） stimulated by Leptospira interrogans and Borrelia burgdorferi. METHODS： Rat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Pudfied Kupffer cells were tested in vitro with alive *** and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies. RESULTS： B. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection. CONCLUSION： Both genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.

关键词： Leptospira interrogans Borrelia burgdorferi Inducible nitric oxide synthase Nitric oxide Reactive oxygen species Kupffer cells Chemiluminescence

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

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Journal of Animal Science and Biotechnology 2023年第1期14卷 152-165页

作者： Anna Rita Piras Federica Ariu Alessio Maltana Giovanni Giuseppe Leoni Nicola Antonio Martino antonella Mastrorocco Maria Elena Dell’Aquila Luisa Bogliolo Department of Veterinary Medicine University of Sassari07100 SassariSardiniaItaly. Department of Biosciences Biotechnologies&BiopharmaceuticsUniversity of Bari Aldo Moro70125 BariItaly.

Background:Heavy metal cadmium(Cd)is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and *** has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization,through oxidative stress ***(Res)is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality ***,we explored whether the addition of Res to in vitro maturation(IVM)medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and ***,we evaluated the effect of supplementing IVM medium with two different Res concentrations(1and 2μmol/L)on nuclear maturation and fertilization of oocytes matured under CdCl2(2μmol/L)***,the concentration of 1μmol/L Res was selected to analyse the effects of this compound on intracellular ROS levels,mitochondrial(mt)distribution and activity,chromatin configuration,cytoskeleton morphology,cortical granules(CGs)distribution and mRNA expression of genes associated with cellular response to oxidative stress(***1,SOD 1,GPX1,GSR,CAT)in Cd-exposed in vitro matured ***:We found that 1μmol/L Res restored the reduced oocyte meiotic competence induced by Cd exposure as well as,Res sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization at both tested ***,we demonstrated that 1μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species(ROS)accumulation,preventing mt dysfunction,maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1,SOD1 and GPX1 ***:Taken together,our findings highlighted the beneficial influence exerted by Res in preventing Cdinduced disturbance of

关键词： Cadmium In vitro maturation Oocyte Ovine Resveratrol

Human leukocyte antigen DQ2/8 prevalence in non-celiac patients with gastrointestinal diseases

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World Journal of Gastroenterology 2013年第16期19卷 2507-2513页

作者： Daniel DiGiacomo antonella Santonicola Fabiana Zingone Edoardo Troncone Maria Cristina Caria Patrizia Borgheresi Gianpaolo Parrilli Carolina Ciacci Gastrointestinal Unit University Federico Ⅱ Naples Department of Medicine Celiac Disease Center Columbia University Celiac Center Loreto Crispi Hospital Celiac Center Gastrointestinal Unit San Giovanni di Dio e Ruggi d'Aragona Hospital University of Salerno Department of Medicine and Surgery Campus di Baronissi University of Salerno Medical School

AIM: To investigate the prevalence of human leukocyte antigen (HLA) DQ2/8 alleles in Southern Italians with liver and gastrointestinal (GI) diseases outside of celiac disease. METHODS: HLA DQ2/8 status was assessed in 443 patients from three ambulatory gastroenterology clinics in Southern Italy (University of Federico Ⅱ, Naples, Loreto Crispi Hospital, Ruggi D'Aragona Hospital, Salerno). Patients were grouped based on disease status [pre-post transplant liver disease, esophageal/gastric organic and functional diseases, irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD)] and DQ2/8 alleles, which correspond to a celiac disease genetic risk gradient. Subject allele frequencies were compared to healthy Italian controls. RESULTS: One hundred and ninety-six out of four hundred and forty-three (44.2%) subjects, median age 56 years and 42.6% female, were DQ2/8 positive. When stratifying by disease we found that 86/188 (45.7%) patients with liver disease were HLA DQ2/8 positive, 39/73 (53.4%) with functional upper GI diseases and 19/41 (46.3%) with organic upper GI diseases were positive. Furthermore, 38/105 (36.2%) patients with IBS and 14/36 (38.9%) with IBD were HLA DQ2/8 positive (P = 0.21). Compared to healthy controls those with functional upper GI diseases disorders had a 1.8 times higher odds of DQ2/8 positivity. Those with liver disease had 1.3 times the odds, albeit not statistically significant, ofDQ2/8 positivity. Both those with IBS and IBD had a lower odds of DQ2/8 positivity compared to healthy controls. CONCLUSION: The proportion of individuals HLA DQ2/8 positive is higher in those with liver/upper functional GI disease and lower in IBS/IBD as compared to general population estimates.

关键词： Human leukocyte antigen DQ2/8 Gastrointestinal and liver disease Celiac disease

The Distribution of an Index of Dissimilarity for Two Samples from a Uniform Population

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Applied Mathematics 2013年第7期4卷 1028-1037页

作者： Giovanni Girone antonella Nannavecchia Faculty of Economics University of Bari Bari Italy

In this paper the authors study the sample behavior of the Gini’s index of dissimilarity in the case of two samples of equal size drawn from the same uniform population. The paper present the analytical results obtained for the exact distribution of the index of dissimilarity for sample sizes n ≤ 8. This result was obtained by expressing the index of dissimilarity as a linear combination of spacings of the pooled sample. The obtained results allow to achieve the exact expressions of the moments for any sample size and, therefore, to highlight the main features of the sampling distributions of the index of dissimilarity. The present study can enhance inferential statistical aspects about one of the main contributions of Gini.

关键词： Index of Dissimilarity Uniform Distribution Spacings

Robustness and Statistical Significance of PAM-like Matrices for Cognate Identification

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通讯和计算机（中英文版） 2010年第12期7卷 21-31页

作者： antonella Delmestri Nello Cristianini Department of Information Engineering and Computer Science University of Trento Trento 38123 Italy Intelligent Systems Laboratory University of Bristol Bristol BS8 1 UB U.K.

End points of clinical trials in metastatic castration-resistant prostate cancer:A systematic review

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World Journal of Methodology 2014年第2期4卷 123-132页

作者： Giuseppe Colloca antonella Venturino Division of Medical Oncology ＂Giovanni Borea＂ HospitalI-18038 SanremoItalyIlaria GovernatoDivision of Medical OncologySanto Spirito Hospital00186 RomaItaly

AIM： To review the defnition and performance of the commonly used end points in trials of systemic thera-pies in metastatic castration-resistant prostate cancer patients. METHODS： A literature search was undertaken on PubMed database to identify studies meeting estab-lished criteria, with the aim of selecting randomized clinical trials and study definition and performance of their end points. The end points were grouped into three categories： overall survival （OS）, time-to-event end points, and response end points. A special analysis was performed for secondary end points of the studies which documented a beneft in OS in the experimental arm. Finally, publishes analyses for surrogacy of the in-cluded end points were also reported. RESULTS： OS, time-to-event and response end points in 31 selected trials were analyzed. OS was the pri-mary end point in 14 trials, and the secondary end point in 17. A time-to-event end point was the primaryend point in 8 studies, and the secondary end point in 22; the most reported time-to-event end points were composite end points, and the events changed among trials. A response end point was the primary end point in 9 studies, in 3 it was prostate-specifc antigen （PSA）-related, in 3 pain-related and in 3 mixed. A response end point was the secondary end point in 19 studies： PSA response and radiologic response were the most frequently used secondary end points in 19 and 11 tri-als, respectively, while pain response was used in 5 ***： A homogeneous defnition of progres-sion in future trials is mandatory. Among response end points, pain-response and PSA-response appear to be the most reliable.

关键词： Metastatic castration-resistant prostate cancer End points Progression-free survival Prostate-specifc antigen Chemotherapy Palliative response

An eighteen-month follow-up study on the effects of Intravitreal Dexamethasone Implant in diabetic macular edema refractory to anti-VEGF therapy

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International Journal of Ophthalmology(English edition) 2016年第10期9卷 1427-1432页

作者： Fernanda Pacella Maria Rosaria Romano Paolo Turchetti Giovanna Tarquini Anna Carnovale antonella Mollicone Alessandra Mastromatteo Elena Pacella Department of Sense Organs Faculty of Medicine and DentistrySapienza University of Rome IRCCS NeuromedPozzilli National Institute for Health MigrationPoverty(INMP/NIHMP) Department of Internal Medicine and Clinical Specialities Sapienza University of Rome

AIM： To evaluate the long-term efficacy and safety of dexamethasone implants in subjects affected by diabetic macular edema（DME） resistant to anti-vascular endothelial growth factor（VEGF） ***： Thirty-two DME patients were enrolled.A700 microgram slow release Intravitreal Dexamethasone Implant（Ozurdex~） was placed in the vitreous *** patients were followed for 18 ***-corrected visual acuity（BCVA） measured with Early Treatment Diabetic Retinopathy Study（ETDRS） and central macular thickness（CMT） exams were carried out at baseline（T0）and after 1（T1）,3（T3）,4（T4）,6（T6）,9（T9）,12（T12）,15（T15）,and 18mo（T18） post injection. RESULTS： Repeated measures ANOVA showed an effect of treatment on ETDRS（P〈0.0001）.Post hoc analyses revealed that ETDRS values were significantly increased at T1,T3,T4,T9,and T15（P 〈0.001） as compared to baseline value（T0）.At T6,T12,and T18,ETDRS values were still statistically higher than baseline（P〈0.001 vs T0）.However,at these time points,we observed a trend to return to baseline *** also showed an effect of treatment（P 〈0.0001）.CMT decreased significantly at T1,T3,T4,T9,and T15（P〈0.001）.At T6（P〈0.01）,T12 and T18（P〈0.001） CMT was also significantly lower than T0 although a trend to return to the baseline conditions was also ***： Our findings demonstrate that Intravitreal Dexamethasone Implant is a good option to improveBCVA and CMT in DME patients resistant to anti-VEGF *** data also show that the use of drugs administered directly into the vitreous allows achieving appropriate and long-lasting concentration at the site of disease without systemic side effects.

关键词： diabetic macular edema Intravitreal Dexamethasone Implant anti-vascular endothelial growth factor therapy

EMI Reduction in Low Input Ripple DC-DC Converter using Chaos PWM technique

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Journal of Electronic Research and Application 2018年第2期2卷 42-47页

作者： antonella Ragusa Giuseppe Marsala National Council of Research ISSIA UOS di PalermoPalermoItaly

This paper presents a chaotic PWM switching technique for a high boost DC-DC converter with a very low ripple at the input *** use of a choatic PWM allows a reduction of Electromagnetic In(EMI)at the output of power converters by spreading the energy spectrum of the output *** this paper a chaotic PWM,using the Chua oscillator,has been implemented in Matlab for the converter under study and a comparison with a traditional PWM control has been done.A detailed description of the generation of the chaotic carrier is presented and the effect on the output EMI mitigation has been shown.

关键词： EMI Chaotic PWM Spreading Spectrum DC-DC converter

Costing and Pricing in Healthcare Private Firms

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Journal of Modern Accounting and Auditing 2014年第7期10卷 764-776页

作者： antonella Cugini Silvia Pilonato University ofPadova Padova Italy

This article analyzes the development of an activity-based costing （ABC） system in a private Italian healthcare firm. Findings shed light on the role of the cost accounting system in price assessment： It outlines new opportunities for the identification of the service mix offered as well as for price optimization. Because private companies provide services both within the public system--where the National Health System （NHS） provides fixed reimbursement fees--and within the private system--where prices are defined by each company. Accurate cost information is extremely important to support managers in the analysis of the service mix profitability offered in each system. For the business segment in which companies do not have flexibility in setting prices, cost information enables managers to identify services which may present profitability problems. For the business segment where price flexibility exists, cost information supports the identification of pricing errors so that charges can be better defined.

关键词： healthcare company cost accounting activity-based costing (ABC) pricing