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The Effect of Electromagnetic Fields with the Mg²⁺ Cyclotron Frequency on Mouse Reproductive Performance

Journal of Electromagnetic Analysis and Applications 2016年第7期8卷 115-123页

作者： Gabriele Gerardi antonella de ninno Vanni Ferrari Sandro Mazzariol Daniele Bernardini Severino Segato department of Animal Medicine Production and Health University of Padova Legnaro (PD) Italy ENEA C.R. Frascati Department Fusion and Nuclear Technologies Technologies for Safety and Health Division Frascati (Roma) Italy department of Comparative Biomedicine and Food Science University of Padova Legnaro (PD) Italy

The present study is aimed to test whether exposure to electromagnetic fields of very weak intensity (≤1 mT) and low frequency (≤100 Hz) may influence reproductive performance and induce teratogenesis in mice. We speculate that a resonant effect occur when the applied frequency matches the cyclotron frequency of Mg2+ (≈60 Hz) involved in the cell duplication. Four groups of mice (four dams and one male each) were exposed to ?50 μT electromagnetic field continuous irradiation of for 100 days. A control group (four dams and one male) was also examined. The exposed dams exhibited a significantly lower number of offspring per birth than the control ones (11.0 vs. 11.6;P = 0.006). A significantly lower average daily gain of body weight per mouse was observed (0.74 vs. 0.77 g/d;P = 0.002), resulting in a reduction of the average body weight per nest at 11 days of age (404 vs. 463 g;P = 0.048). Post mortem examinations revealed a significant increase in mild chronic hepatic inflammatory findings (28 vs. 0%;P = 0.001) in the offspring and myocardial hypertrophy (25 vs. 0%;P = 0.023) in the dams. The exposure of mice to an electromagnetic field with the cyclotron frequency of Mg2+ during pregnancy caused a measurable effect on the reproductive performance in terms of offspring per birth. This finding may be considered as a warning about the environmental effects of the electromagnetic fields on the stability of individual species and ecosystems.

关键词： ELF-EMF Cyclotron Frequency Mouse Birth-Rate Postnatal Growth Histological Findings

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Controlling DNA Bundle Size and Spatial Arrangement in Selfassembled Arrays on Superhydrophobic Surface

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Nano-Micro Letters 2015年第2期7卷 146-151页

作者： Gabriele Ciasca Massimiliano Papi Valentina Palmieri Michela Chiarpotto Simone Di Claudio Adele de ninno Ennio Giovine Gaetano Campi Annamaria Gerardino Luca Businaro Marco de Spirito Istituto di Fisica Universitá Cattolica SC L.go Francesco Vito 100168 Rome Italy Istituto di Fotonica e Nanotecnologie-CNR Via Cineto Romano 42 00156 Rome Italy Institute of Crystallography-CNR Via Salaria Km 290016 Monterotondo Rome Italy

The use of superhydrophobic surfaces(SHSs) is now emerging as an attractive platform for the realization of one-dimensional(1D) nanostructures with potential applications in many nanotechnological and biotechnological *** this purpose, a strict control of the nanostructures size and their spatial arrangement is highly required. However, these parameters may be strongly dependent on the complex evaporation dynamics of the sessile droplet on the SHS. In this work, we investigated the effect of the evaporation dynamics on the size and the spatial arrangement of self-assembled 1D DNA bundles. Our results reveal that different arrangements and bundle size distributions may occur depending on droplet evaporation stage. These results contribute to elucidate the formation mechanism of 1D nanostructures on SHSs.

关键词： Superhydrophobicity DNA array Cassie state Wenzel state 1D nanostructures Self-assembly

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Controlling the Cassie-to-Wenzel Transition: an Easy Route towards the Realization of Tridimensional Arrays of Biological Objects

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Nano-Micro Letters 2014年第3期6卷 280-286页

作者： G.Ciasca M.Papi M.Chiarpotto A.de ninno E.Giovine G.Campi A.Gerardino M.de Spirito L.Businaro Istituto di Fisica Universit'a Cattolica S. Cuore L.go Francesco Vito 1 I-00168 Roma Italy Istituto di Fotonica e Nanotecnologie - CNR Via Cineto Romano 42 Roma 00156 Italy Institute of Crystallography – CNR Via Salaria Km 29 0016 Monterotondo Roma Italy

In this paper we provide evidence that the Cassie-to-Wenzel transition, despite its detrimental effects on the wetting properties of superhydrophobic surfaces, can be exploited as an effective micro-fabrication strategy to obtain highly ordered arrays of biological objects. To this purpose we fabricated a patterned surface wetted in the Cassie state, where we deposited a droplet containing genomic DNA. We observed that, when the droplet wets the surface in the Cassie state, an array of DNA filaments pinned on the top edges between pillars is formed. Conversely, when the Cassie-to-Wenzel transition occurs, DNA can be pinned at different height between pillars. These results open the way to the realization of tridimensional arrays of biological objects.

关键词： Superhydrophobic patterned surfaces Cassie-to-Wenzel transition DNA arrays

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