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The effect of protein oxidation on the formation of advanced glycation end products after chicken myofibrillar protein glycation

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Food Science and Human Wellness 2023年第5期12卷 1571-1579页

作者： Zongshuai Zhu Anthony Pius Bassey Ming Huang Iftikhar Ali Khan College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China National R&D Center for Poultry Processing Technology Nanjing Huangjiaoshou Food Science and Technology Co.Ltd.Nanjing 211200China Institution of Agricultural Products Processing Jiangsu Academy of Agricultural SciencesNanjing 210095China

Investigation that protein oxidation to the formation of advanced glycation end products(AGEs)after chicken myofibrillar protein glycation is limited.Models of protein oxidation induced by different concentrations of hydroxyl radicals(·OH)were developed after the chicken myofibrillar protein mild glycation(MPG).Results exhibited that levels of AGEs and surface hydrophobicity(H_(0))steadily increased with the a ddition of h ydrogen peroxide(H_(2)O_(2))concentration.However,levels of s ulfhydryl group,free amino group,and particle size gradually decreased with the H_(2)O_(2)concentration.The protein carbonyl value increased in H_(2)O_(2)concentration until 10 mmol/L.Pearson's correlation indicated that MPG structure modification(unfolding and degradation)induced by protein oxidation were significantly positively correlated with AGEs concentration(P<0.05).Finally,a mechanism was proposed to hypothesize t he effect of protein oxidation on the formation of AGEs under MPG conditions.

关键词： Protein oxidation Glycated myofibrillar protein Structure changes Advanced glycation end products

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Glyoxal induced advanced glycation end products formation in chicken meat emulsion instead of oxidation

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Food Science and Human Wellness 2022年第4期11卷 828-836页

作者： Rui Fang Zongshuai Zhu Anthony Pius Bassey Iftikhar Ali Khan Ming Huang College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China Center Testing International Pinbiao(Jiangsu) Certifi cation Technology Co.Ltd.Nanjing 210046China Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced StudyShenzhen UniversityShenzhen 518060China Nanjing Huangjiaoshou Food Science and Technology Co.Ltd. Jiangsu Research Center for Livestock and Poultry Products Processing Engineering TechnologyNanjing 211200China

Advanced glycation end products(AGE) are potential harmful substances formed in the advanced Maillard reaction and increasingly investigated in muscle foods. However, the contribution of oxidation to the AGE formation is controversial. Moreover, reports on glyoxal(GO) induced AGE formation in chicken meat emulsion(CME) are limited. Thus, the effects of GO on emulsifying properties, rheological behavior and AGE formation in CME were investigated. Our findings exhibited that levels of Nε-carboxymethyllysine(CML) and Nε-carboxyethyllysine(CEL) were associated with lipid oxidation but not significantly(P > 0.05). Levels of AGE peaked when GO concentration ranged from 5 mmol/L(CML) to 10 mmol/L(CEL). The droplets’ aggregation associated with the disulfide bond when the concentration of GO was at 0.5–30 mmol/L while non-disulfide bond association occurred at 30–50 mmol/L GO concentration. In conclusion, compared to the effect of oxidation, GO exhibited the main role in the AGE formation of CME. This study will provide theoretical significance for further understanding and controlling the formation of AGE in CME.

关键词： Glyoxal Emulsion Oxidation Advanced glycation end products Chicken meat

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Evaluation of co-spoilage potential of Pseudomonas fragi and Pseudomonas lundensis isolated from chilled pork: in vitro and in situ

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Food Science and Human Wellness 2024年

作者： Han Dou Anthony Pius Bassey Jingwen Li Xianming Zeng Keping Ye State Key Laboratory of Meat Quality Control and Cultured Meat Development Jiangsu Collaborative Innovation Center of Meat Production and Processing College of Food Science and Technology Nanjing Agricultural University

Pseudomonas (P.) fragi and P. lundensis have been reported as key spoilage bacteria in aerobically-stored chilled meat. However， the co-spoilage effect of these bacteria has not been effectively evaluated. This study evaluated the spoilage potential of P. fragi， P. lundensis and P. fragi + P. lundensis in vitro and in situ at 4 ℃. The results showed that P. fragi + P. lundensis performed the highest growth rate and displayed larger decomposition zone diameters on Raw-pork juice agar (RJA) plates. P. fragi + P. lundensis inoculants exhibited the strongest proteolytic activity， which resulted in the highest values of trichloroacetic acid (TCA)-soluble peptides， total volatile basic nitrogen (TVB-N) and myofibril fragmentation index (MFI) in chilled pork. Moreover， the inoculated samples showed different pH and sensory changes. Notably， increased amounts of volatile organic compounds (VOCs)， such as octanal， nonanal， 2-nonanone， 1-propanol， 1-octanol， isopropyl acetate， and 2，6-dimethylpyazine， were observed in inoculated P. fragi + P. lundensis samples， hinting their potential use as spoilage markers for spoilage monitoring of co-cultures. This study would provide a deeper understanding of meat spoilage and serve as a reference for future studies to inhibit meat spoilage.

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Bacterial Isolates and Their Antibiotic Susceptibility Pattern among Patients with Infected Wounds Admited in Orthopaedic and Trauma Ward in Tertiary Care Hospital North-Eastern Tanzania

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Journal of Biosciences and Medicines 2022年第7期10卷 83-96页

作者： Lee Kapalisya Mwakalinga Rogers Joachim Temu pius Gerald Horumpende Nicolaus Ngowi anthony Japhet Pallangyo Department of Orthopedic and Traumatology Facult of Medicine Kilimanjaro Christian Medical University College Moshi Tanzania Department of Orthopedics and Traumatology Kilimanjaro Christian Medical Center Moshi Tanzania Kilimanjaro College of Health and Allied Sciences Moshi Tanzania Department of Biochemistry and Molecular Biology Kilimanjaro Clinical Research Institute Moshi Tanzania Department of Biochemistry and Molecular Biology Kilimanjaro Christian Medical Center Moshi Tanzania Lugalo General Military Hospital Military College of Medical Sciennces Dar es Salaam Tanzania

The hospital environment contributes to wound infections. Effects of such infections include prolonged hospitalization, increased morbidity, potential for antibiotics resistance and mortality due to sepsis. An updated knowledge of antibiotics susceptibility profiles of clinical isolates will assist both in choosing the most appropriate antibiotic treatment for wound infections and help in curbing the escalation of drug resistance. Cross sectional hospital based study, analysis of 125 pus samples collected from January 2018 to December 2020 was conducted. Identification and characterization of isolates were performed on the basis of Gram staining, microscopic characteristics, colony characteristic, and biochemical tests using standard microbiological methods. Antibiotic susceptibilities of bacterial isolates were determined by Kirby Bauer disc diffusion. A total of 125 pus samples were studied, 94 (75%) were from male patients, mean age was 38.5 (SD ± 19) years. Single bacterial isolates were recovered from 120 (96%) samples, 67 (53.6%) shows Multiple Drug Resistance (MDR) pattern, 74 (59.2%) were gram negative, the predominant organism isolated was Staphylococcus aureus 46 (36.8%). Gram negative isolates showed high resistance to ampicillin and cephalosporins. Gram positive isolates showed high resistance to erythromycin. Both gram positive and negative were found to be highly susceptible to gentamicin, amikacin, clindamycin, ciprofloxacin and amoxicillin/clavulanic acid. The study showed that Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli are the major bacteria isolated from pus samples. High proportion (53.6%) of the isolates was MDR. In the light of these findings, a change in antibiotic prescription policy is required at this hospital.

关键词： Pus Bacteria Antibiotic Tanzania

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Up-regulation of oxidative stress and inflammation in the brain of albino wistar rats following sub-acute administration of Synclisia scabrida root extract

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TMR Integrative Medicine 2022年第30期6卷 1-7页

作者： Emmanuel Uchechukwu Modo Ijeoma Nina Eke Utibe Evans bassey anthony Chibuzor Nnamudi Ekam Ime Akpakpan Okon Effiom Etim Iheanyichukwu Wopara Prosper Great Legborsi Department of Biochemistry Faculty of Basic Medical SciencesPAMO University of Medical SciencesPort HarcourtRivers StateNigeria Department of Biochemistry Faculty of SciencesUniversity of Port HarcourtPort HarcourtRivers StateNigeria

Objective: Synclisia scabrida is a medicinal plant used over the years for the treatment ofseveral medical conditions yet there is paucity of information on its systemic and organspecific toxicity. Consequently, sub-acute neurotoxicity of root extract of Synclisia scabridawas evaluated in albino Wistar rats. Methods: Thirty male albino rats with average weightof 140g were randomized into 5 groups consisting of 6 rats in each group. Group 1 was thecontrol while 50 mg/kg, 100 mg/kg, 200 mg/kg and 400 mg/kg of the root extract wereadministered to Groups 2, 3, 4 and 5 respectively for 28 days. Malondialdehyde,glutathione, nitric oxide, protein, tumour necrosis factor-α, acetylcholine, catalase andacetylcholinesterase levels were measured in brain homogenates. Body weight of theanimals and histology of the hippocampus and cerebral cortex were evaluated. Results:Root extract of Synclisia scabrida was observed to increase malondialdehyde concentrationand decrease antioxidants biomarkers when compared with the control. Significantly(p<0.05) increased TNF-α concentration and acetylcholinesterase activity caused by theextract when compared with the control was observed. The concentration of acetylcholinesignificantly decreased in Synclisia scabrida treated groups in comparison with the control.The histomorphology of the hippocampus and cerebral cortex revealed pyknotic pyramidalneurons in Synclisia scabrida treated rat relative to the control with normal pyramidalneurons. The body weight of the extract treated groups were significantly decreased whencompared to the control Group. Conclusion: The study has demonstrated that the rootextract of Synclisia scabrida induces and up-regulates oxidative stress and inflammation inthe brain of male albino Wistar rat coupled with reduced acetylcholine concentration hencethe extract possesses neurotoxic potentials.

关键词： Synclisia scabrida oxidative stress antioxidant inflammation neurotoxicity

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