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In VivoStudy of Healing Effects of Sida acuta Leaf Extracts on Helicobacter pyloriInduced Ulceration in Mice

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Journal of Biosciences and Medicines 2020年第9期8卷 1-14页

作者： Chito Clare Ekwealor Chidinma Chinwendu Okorie Chinwe Clarice Ukoha anthonia nkiruka mba Department of Applied Microbiology & Brewing Nnamdi Azikiwe University Awka Nigeria Department of Microbiology Chukwuemeka Odimegwu Ojukwu University Uli Nigeria

Helicobacter pylori is a human pathogen that is widely distributed and known to cause peptic ulcer, gastritis and gastric cancer. Treatment is usually by combination of acid inhibitory therapy and antibiotics because of drug resistance problems. Studies now focus on the use of medicinal plants for treatment of H. pylori induced ulcers because they are inexpensive and have limited side effects. This study was undertaken to determine the effects of cold water and ethanol extracts of Sida acuta leaves on H. pylori induced ulcer in mice. Oral administration with the aid of a feeding tube of 0.5 ml of 107 CFU/ml of H. pylori (Accession number LT799736) in phosphate buffer saline (pH 6.8) was used to induce ulcer in mice. Effects of various concentrations of cold water and ethanol extracts of S. acuta leaves on the ulcer parameters and histology examinations of stomach tissues of mice were investigated after 2 weeks of administration of extracts into the mice. Mice treated with clarithromycin served as positive control while mice administered with H. pylori but not treated served as negative control. One-way analysis of variance (ANOVA) was used for data analysis and results were considered significant if p 6 CFU/ml to 3.5 × 106 CFU/ml and 2.6 × 106 CFU/ml for cold water and ethanol extracts respectively. Reduction in ulcer severity (2.00 ± 0.10 to 0.080 ± 0.05), ulcer index (8.50 ± 1.10 to 4.26 ± 0.03) and gastric volume (1.32 ± 0.12 to 0.54 ± 0.13) were observed. Histology of stomach tissues of mice treated with extracts revealed clear granulation indicating tissue repair and wound healing. S. acuta extracts were observed to enhance ulcer healing in a mice model.

关键词： Helicobacter pylori Sida acuta Leaves Peptic Ulcer Ethanol Extract

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Bacteriological Assessment and Antibiotic Susceptibility Profile of Bacteria Recovered from Clarias gariepinusSelected from Various Fish Farms in Anambra North Senatorial Zones in Anambra State, Nigeria

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Advances in Microbiology 2021年第5期11卷 243-256页

作者： Chioma M. Ogbukagu Chikodili G. Anaukwu Chito C. Ekwealor anthonia N. mba Ikechukwu A. Ekwealor Department of Applied Microbiology and Brewing Nnamdi Azikiwe University Awka Nigeria

Fishes are cheap sources of protein and are commonly reared in artificial pond in Nigeria. Bacterial infested fishes have been a serious public health concern. This study was aimed at isolating and identifying bacteria associated with the internal organs (gills, liver, guts) of Clarias gariepinus (African Catfish) in fish ponds in Anambra North Senatorial zones in Anambra State, Nigeria, and to determine the seasonal bacterial load of the organs and antibiotic susceptibility profile of the isolates to conventional antibiotics. A total of 720 African catfish were sampled from randomly selected fish farms during rainy and dry seasons. Bacteria isolates inoculated on Blood and Nutrient agar plates were identified based on morphological and biochemical characteristics. Susceptibility of the isolates to antibiotics was carried out using the Kirby-Bauer disc diffusion method. Isolates from the fish organs include species of Vibrio, Aeromonas, Pseudomonas, Lactobacillus, Staphylococcus, Microbacterium, Serratia, Proteus, Bacillus, Streptococcus, Citrobacter and Micrococcus. Results also revealed that there was significant difference (p value Bacillus sp. was the most abundant gram positive bacterium in the organs of the fish samples during the rainy and dry season, while Citrobacter sp., a gram negative organism, was relatively abundant in the liver and gut. Percentage susceptibility of the bacteria to antibiotics was highest with vancomycin (100%) and least with erythromycin (75.0%). The most susceptible isolates were species of Staphylococcus, Microccoccus and Microbacterium while Bacillus sp. was the most resistant bacterium. This study has shown that high bacteria load are found in the internal organs of the fish and a good number of the bacteria are resistant to some of the antibiotics tested. There is, therefore, need for

关键词： Clarias gariepinus Antibiotic Susceptibility Bacteria Fish Organs

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