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Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

world Journal of Stem Cells 2020年第12期12卷 1640-1651页

作者： Cintia DS Horinouchi María Julia Barisón anny w robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna Stem Cells Basic Biology Laboratory Instituto Carlos ChagasCuritiba 81350010ParanáBrazil

BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several ***,for successful clinical application,it is critical that high-quality cells can be *** factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as ***,there is no consensus regarding this characteristic,and there is conflicting information in the *** To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young *** hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow *** doubling time was assessed over seven *** adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic *** potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic *** expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain *** hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old ***,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal *** average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation pot

关键词： Adipose-derived stem cells Stem cells Adipogenesis Osteogenesis Cell differentiation Donor age

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Multi-Scale Dilated Convolution Network for SPECT-MPI Cardiovascular Disease Classification with Adaptive Denoising and Attenuation Correction

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 299-327页

作者： A.robert Singh Suganya Athisayamani Gyanendra Prasad Joshi Bhanu Shrestha Department of Computational Intelligence SRM Institute of Science and TechnologyKattankulathur603203Tamil NaduIndia School of Computing Sastra Deemed to be UniversityThanjavur613401Tamil NaduIndia Department of Artificial Intelligence&Software Kangwon National UniversitySamcheok25913Republic of Korea Department of Information Convergence System Graduate School of Smart ConvergenceKwangwoon UniversitySeoul01897Republic of Korea

Myocardial perfusion imaging(MPI),which uses single-photon emission computed tomography(SPECT),is a well-known estimating tool for medical diagnosis,employing the classification of images to show situations in coronary artery disease(CAD).The automatic classification of SPECT images for different techniques has achieved near-optimal accuracy when using convolutional neural networks(CNNs).This paper uses a SPECT classification framework with three steps:1)Image denoising,2)Attenuation correction,and 3)Image *** denoising is done by a U-Net architecture that ensures effective image *** correction is implemented by a convolution neural network model that can remove the attenuation that affects the feature extraction process of ***,a novel multi-scale diluted convolution(MSDC)network is *** merges the features extracted in different scales and makes the model learn the features more *** scales of filters with size 3×3 are used to extract *** three steps are compared with state-of-the-art *** proposed denoising architecture ensures a high-quality image with the highest peak signal-to-noise ratio(PSNR)value of *** proposed classification method is compared with the five different CNN models,and the proposed method ensures better classification with an accuracy of 96%,precision of 87%,sensitivity of 87%,specificity of 89%,and F1-score of 87%.To demonstrate the importance of preprocessing,the classification model was analyzed without denoising and attenuation correction.

关键词： SPECT-MPI CAD MSDC denoising attenuation correction classification

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Three-Stage Transfer Learning with AlexNet50 for MRI Image Multi-Class Classification with Optimal Learning Rate

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 155-183页

作者： Suganya Athisayamani A.robert Singh Gyanendra Prasad Joshi woong Cho School of Computing Sastra Deemed to be UniversityThanjavur613401India Department of Computational Intelligence SRM Institute of Science and TechnologyKattankulathur603203India Department of AI and Software Engineering Kangwon National UniversitySamcheok25913Republic of Korea Department of Electronics Information and Communication EngineeringKangwon National UniversitySamcheok25913Republic of Korea

In radiology,magnetic resonance imaging(MRI)is an essential diagnostic tool that provides detailed images of a patient’s anatomical and physiological *** is particularly effective for detecting soft tissue ***,radiologists manually interpret these images,which can be labor-intensive and time-consuming due to the vast amount of *** address this challenge,machine learning,and deep learning approaches can be utilized to improve the accuracy and efficiency of anomaly detection in MRI *** manuscript presents the use of the Deep AlexNet50 model for MRI classification with discriminative learning *** are three stages for learning;in the first stage,the whole dataset is used to learn the *** the second stage,some layers of AlexNet50 are frozen with an augmented dataset,and in the third stage,AlexNet50 with an augmented dataset with the augmented *** method used three publicly available MRI classification datasets:Harvard whole brain atlas(HwBA-dataset),the School of Biomedical Engineering of Southern Medical University(SMU-dataset),and The National Institute of Neuroscience and Hospitals brain MRI dataset(NINS-dataset)for *** hyperparameter optimizers like Adam,stochastic gradient descent(SGD),Root mean square propagation(RMS prop),Adamax,and Adamw have been used to compare the performance of the learning ***-dataset registers maximum classification *** evaluated the performance of the proposed classification model using several quantitative metrics,achieving an average accuracy of 98%.

关键词： MRI tumors classification AlexNet50 transfer learning hyperparameter tuning optimizer

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Human pluripotent stem cell-derived extracellular vesicles:From now to the future

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world Journal of Stem Cells 2023年第5期15卷 453-465页

作者： Bruno Moises de Matos Marco Augusto Stimamiglio Alejandro Correa anny waloski robert Stem Cells Basic Biology Laboratory Carlos Chagas InstituteCuritiba 81350010ParanáBrazil

Extracellular vesicles(EVs)are nanometric particles that enclose cell-derived bioactive molecules in a lipid bilayer and serve as intercellular communication ***,in various biological contexts,EVs are reported to engage in immune modulation,senescence,and cell proliferation and ***,EVs could be key elements for potential off-the-shelf cell-free *** has been studied regarding EVs derived from human pluripotent stem cells(hPSC-EVs),even though hPSCs offer good opportunities for induction of tissue regeneration and unlimited proliferative *** this review article,we provide an overview of studies using hPSC-EVs,focusing on identifying the conditions in which the cells are cultivated for the isolation of EVs,how they are characterized,and applications already *** topics reported in this article highlight the incipient status of the studies in the field and the significance of hPSC-EVs’prospective applications as PSC-derived cell-free therapy products.

关键词： Pluripotent stem cells Extracellular vesicles Exosome Cell-free therapy

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Human endogenous retrovirus type-w and multiple sclerosis–related smoldering neuroinflammation

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Neural Regeneration Research 2025年第3期20卷 813-814页

作者： Joel Gruchot Laura Reiche Andrew Chan robert Hoepner Patrick Küry Department of Neurology Medical Faculty and University Hospital DüsseldorfHeinrich Heine University DüsseldorfDüsseldorfGermany Department of Neurology InselspitalBern University Hospital and University of BernBernSwitzerland

Introduction to human endogenous retrovirus type-w(HERV-w): Genomic inheritance from the past includes retroviral sequences that have been stably incorporated into our genomes and account for up to 8% of human DNA.

关键词： endogenous inflammation Human

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Lipid droplets in the nervous system:involvement in cell metabolic homeostasis

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Neural Regeneration Research 2025年第3期20卷 740-750页

作者： Yuchen Zhang Yiqing Chen Cheng Zhuang Jingxuan Qi robert Chunhua Zhao Jiao wang School of Life Sciences Shanghai UniversityShanghaiChina School of Medicine Shanghai UniversityShanghaiChina Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic MedicinePeking Union Medical CollegeBeijingChina Center of Excellence in Tissue Engineering Chinese Academy of Medical SciencesBeijingChina Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy BeijingChina

Lipid droplets serve as primary storage organelles for neutral lipids in neurons,glial cells,and other cells in the nervous *** droplet formation begins with the synthesis of neutral lipids in the endoplasmic ***,lipid droplets were recognized for their role in maintaining lipid metabolism and energy homeostasis;however,recent research has shown that lipid droplets are highly adaptive organelles with diverse functions in the nervous *** addition to their role in regulating cell metabolism,lipid droplets play a protective role in various cellular stress ***,lipid droplets exhibit specific functions in neurons and glial *** of lipid droplet formation leads to cellular dysfunction,metabolic abnormalities,and nervous system *** review aims to provide an overview of the role of lipid droplets in the nervous system,covering topics such as biogenesis,cellular specificity,and ***,it will explore the association between lipid droplets and neurodegenerative *** the involvement of lipid droplets in cell metabolic homeostasis related to the nervous system is crucial to determine the underlying causes and in exploring potential therapeutic approaches for these diseases.

关键词： Alzheimer's disease lipid droplet biogenesis lipid droplets lipid metabolism nervous system neurodegenerative disorders oxidative stress Parkinson's disease

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Sleep as a window to understand and regulate Alzheimer's disease:emerging roles of thalamic reticular nucleus

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Neural Regeneration Research 2025年第6期20卷 1711-1712页

作者： Haoqi Sun Shiqian Shen robert J.Thomas M.Brandon westover Can Zhang Department of Neurology Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMAUSA Center for Translational Pain Research Department of AnesthesiaCritical Care and Pain MedicineMassachusetis General HospitalHarvard Medical SchoolBostonMAUSA Division of Pulmonary Critical Care and Sleep MedicineDepartment of MedicineBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMAUSA Genetics and Aging Research Unit McCance Center for Brain HealthMass General Institute for Neurodegenerative DiseaseDepartment of NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMAUSA

Introduction:Alzheimer 's disease(AD) is a common neurodegenerative disorder and the primary cause of dementia. Considerable evidence supports the “amyloid hypothesis,” stating that the pathogenesis of AD is primarily caused by the deposition of amyloid-β(Aβ), which drives tau phosphorylation, neuroinflammation, and neurodegeneration in the brain. The amyloid hypothesis is strengthened by the significant and moderate benefit of lecanemab, a humanized antibody through an anti-amyloid mechanism,showing slowed clinical decline(van Dyck et al.,2023). The recent positive results of anti-amyloid trials have brought back focus on the amyloid hypothesis through biochemical, genetic, and pharmacological approaches(Zhang, 2023).

关键词： Alzheimer inflammation hypothesis

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COMPARING TwO CLUSTERING-BASED TECHNIQUES TO TRACK MOBILE NODES IN INDUSTRIAL wIRELESS SENSOR NETwORKS

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Journal of Systems Science and Systems Engineering 2016年第2期25卷 177-209页

作者： Mohammad Gholami robert w. Brennan Department of Mechanical and Manufacturing Engineering Schulich School of Engineering University of Calgary Calgary Alberta Canada T2N 1N4

Tracking mobile nodes in dynamic and noisy conditions of industrial environments has provided a paradigm for many issues inherent in the area of distributed control systems in general and wireless sensor networks in particular. Due to the dynamic nature of the industrial environments, a practical tracking system is required that is adaptable to the changes in the environment. More specifically, given the limited resources of wireless nodes and the challenges created by harsh industrial environments there is a need for a technique that can modify the configuration of the system on the fly as new wireless nodes are added to the network and obsolete ones are removed. To address these issues, two cluster-based tracking systems, one static and the other dynamic, are proposed to organize the overall network field into a set of tracking zones, each composed of a sink node and a set of corresponding anchor nodes. To manage the wireless nodes activities and inter and intra cluster communications, an agent-based technique is employed. To compare the architectures, we report on a set of experiments performed in JADE （Java Agent Development Environment）. In these experiments, we compare two agent-based approaches （dynamic and static） for managing clusters of wireless sensor nodes in a distributed tracking system. The experimental results corroborate the efficiency of the static clusters versus the robustness and effectiveness of the dynamic clusters.

关键词： wireless sensor networks tracking mobile nodes factory automation distributed adaptive systems reconfigurability

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An Empirical Study on the Impact of Automation on the Requirements Analysis Process

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Journal of Computer Science & Technology 2007年第3期22卷 338-347页

作者： Giuseppe Lami robert w.Ferguson Istituto di Scienza e Tecnologie dell'Informazione"Alessandro Faedo" 1-56124 Pisa Italy Software Engineering Institute Carnegie Mellon University 15213 PittsburghPAU.S.A.

Requirements analysis is an important phase in a software project. The analysis is often performed in an informal way by specialists who review documents looking for ambiguities, technical inconsistencies and incomplete parts. Automation is still far from being applied in requirements analyses, above all since natural languages are informal and thus difficult to treat automatically. There are only a few tools that can analyse texts. One of them, called QuARS, was developed by the Istituto di Scienza e Tecnologie dell＇Informazione and can analyse texts in terms of ambiguity. This paper describes how QuARS was used in a formal empirical experiment to assess the impact in terms of effectiveness and efficacy of the automation in the requirements review process of a software company.

关键词： requirements/specifications analysis requirements/specifications tools process metrics

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Motor tract reorganization after acute central nervous system injury: a translational perspective

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Neural Regeneration Research 2021年第6期16卷 1144-1149页

作者： Hajime Takase robert w.Regenhardt Department of Neurosurgery Yokohama City University Graduate School of MedicineYokohamaJapan Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical SchoolCharlestownMAUSA Department of Neurology Massachusetts General Hospital and Harvard Medical SchoolBostonMAUSA

Acute central nervous system injuries are among the most common causes of disability worldwide,with widespread social and economic *** tract injury accounts for the majority of this disability;therefore,there is impetus to understand mechanisms underlying the pathophysiology of injury and subsequent reorganization of the motor tract that may lead to *** acute central nervous system injury,there are changes in the microenvironment and structure of the motor *** example,ischemic stroke involves decreased local blood flow and tissue death from lack of oxygen and *** injury,in contrast,causes stretching and shearing injury to microstructures,including myelinated axons and their surrounding *** involve blood-brain barrier dysfunction,which is an important initial *** acute central nervous system injury,motor tract reorganization occurs in the form of cortical remapping in the gray matter and axonal regeneration and rewiring in the white *** remapping involves one cortical region taking on the role of ***-response-element binding protein is a key transcription factor that can enhance plasticity in the peri-infarct *** regeneration and rewiring depend on complex cell-cell interactions between axons,oligodendrocytes,and other *** RhoA/Rho-associated coiled-coil containing kinase signaling pathway plays a central role in axon growth/regeneration through interactions with myelin-derived axonal growth inhibitors and regulation of actin cytoskeletal *** and their precursors play a role in myelination,and neurons are involved through their voltage-gated calcium *** the pathophysiology of injury and the biology of motor tract reorganization may allow the development of therapies to enhance recovery after acute central nervous system *** include targeted rehabilitation,novel pharmacotherapies,such as growth factors and axonal growt

关键词： corticospinal tract myelin-axon interaction recovery remapping reorganization RhoA/ROCK translation

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