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Characterization of some bacterial strains isolated from the Egyptian eastern and northern coastlines with antimicrobial activity of bacillus zhangzhouensis OMER4

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Acta Oceanologica Sinica 2022年第3期41卷 86-93页

作者： Mohamed O.Soliman Waleed b.Suleiman Mohamed M.Roushdy Eman N.Elbatrawy Ahmed M.gad Department of botany and Microbiology Faculty of ScienceAl-Azhar UniversityCairo 11884Egypt The Regional Center for Mycology and biotechnology Al-Azhar UniversityCairo 11884Egypt Microbiology Laboratory Marine Environmental DivisionNational Institute of Oceanography and FisheriesAlexandria 11865Egypt

Marine microorganisms were considered to be important sources of marine bioactive *** major objective of the study was to isolate and characterize bacteria with antimicrobial activities from the various marine environment of *** this respect,thirty-five bacterial isolates were recovered from sediment samples collected from different spots along the Egyptian Red Sea coastline and Alexandria coastline during the summer season of 2017 and *** to the morphological,physiological,and biochemical characteristics,the bacterial isolates were clustered into 13 groups designated as A,b,…,***,14 Gram-negative and 21 Grampositive bacteria were *** isolated bacterial strains were screened for their potentiality for antimicrobial agent(s)production against ten indicator *** Mo13 was showed high antimicrobial activity against all empirical ***,the most promising marine bacterial isolate with code MO13 was identified as bacillus zhangzhouensis OMER4 according to the phenotypic characterization through morphological,physiological,and biochemical tests as well as genotypic characterization through the 16 S rDNA *** bioactive components were extracted with ethyl acetate,then analyzed using GC-MS and the substantial component was recognized as phenol,2,4-bis(1,1-dimethyl ethyl).

关键词： marine bacteria natural product Egyptian coastline antimicrobial bacillus zhangzhouensis GC-MS characterization

Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids:the potential association with oocyte quality

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Journal of Animal Science and biotechnology 2022年第6期13卷 1727-1742页

作者： Ahmed gad Matej Murin Alexandra bartkova Veronika kinterova katerina Marcollova Jozef Laurincik Radek Prochazka Institute of Animal Physiology and Genetics Czech Academy of Sciences27721 LiběchovCzech Republic Department of Animal Production Faculty of AgricultureCairo UniversityGiza 12613Egypt Department of botany and Genetics Faculty of Natural SciencesConstantine the Philosopher University in Nitra94901 NitraSlovakia.

background: Ovarian follicular fluids(FFs) contain several kinds of regulatory factors that maintain a suitable micro-environment for oocyte development. Extracellular vesicles(EVs) are among the factors that play essential roles in regulating follicle and oocyte development through their cargo molecules that include microRNAs(miRNAs). This study aimed to investigate small-EV(s-EV) miRNAs in porcine FFs and their potential association with oocyte ***: Individual aspirated oocytes were stained with lissamine green b stain(Lb), a vital stain for oocyte quality, and each oocyte was classified as high-quality(unstained;HQ) or low-quality(stained;LQ). FFs corresponding to oocytes were pooled together into HQ and LQ groups. Small-EVs were isolated from FFs, characterized, and their miRNA cargo was identified using the Illumina Nova Seq sequencing platform. Additionally, s-EVs from the HQ and LQ groups were utilized to investigate their effect on oocyte development after co-incubation during in vitro ***: A total of 19 miRNAs(including miR-125b, miR-193a-5p, and miR-320) were significantly upregulated, while 23(including miR-9, miR-206, and miR-6516) were downregulated in the HQ compared to the LQ group. Apoptosis, p53 signaling, and cAMP signaling were among the top pathways targeted by the elevated miRNAs in the HQ group while oocyte meiosis, gap junction, and TGF-beta signaling were among the top pathways targeted by the elevated miRNAs in the LQ group. The supplementation of small-EVs during maturation does not affect the oocyte developmental rates. However, LQ s-EVs increase the proportion of oocytes with homogeneous mitochondrial distribution and decrease the proportion of heterogeneous ***: Our findings indicated that FF-EVs contain different miRNA cargos associated with oocyte quality and could affect the mitochondrial distribution patterns during oocyte maturation.

关键词： Extracellular vesicles Follicular fuids MiRNA Oocyte quality Porcine

Impact of different nano additives on performance, combustion, emissions and exergetic analysis of a diesel engine using waste cooking oil biodiesel

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Propulsion and Power Research 2022年第2期11卷 209-223页

作者： M.S.gad Mostafa M.Abdel Aziz Hatem kayed Mechanical Engineering Department Faculty of EngineeringFayoum UniversityEgypt Mechanical Engineering Department Institute of Aviation Engineering and TechnologyGizaEgypt Mechanical Power Engineering Department Faculty of EngineeringCairo University12613Egypt

biodiesel is derived from waste cooking oil (WCO) by transesterification. Methylester was prepared by mixing diesel and biodiesel oils as 20% by volume. Nano particles asTiO2, Al2O3 and CNTs were blended with biodiesel blend at different concentrations of 25,50, and 100 mg/l to enhance the physicochemical fuel characteristics to obtain clean and effi-cient combustion performance. An experimental setup was incorporated into a diesel engine toinvestigate the influence of these nano-materials on engine performance, exergy analysis, combustion characteristics and emissions using WCO biodiesel-diesel mixture. Enriching methylester mixture with 100 ppm titanium, alumina and CNTs (b20T100, b20A100 andb20C100) increased the thermal efficiency by 4%, 6% and 11.5%, respectively compared tob20. biodiesel blending with nano additives b20T100, b20A100 and b20C100 decreasedthe emissions of CO (11%, 24% and 30%, respectively), HC (8%, 17% and 25%, respectively)and smoke (10%, 13% and 19%, respectively) compared to b20. However, the noticeable increase of NOx was estimated by 5%, 12% and 27% for b20T100, b20A100 and b20C100,respectively. Finally, the results showed the rise in peak cylinder pressure by 5%, 9% and 11% and increase in heat release rate by 4%, 8% and 13% for b20T100, b20A100 andb20C100, respectively. The fuel exergy of b20T100, b20A100 and b20C100 are lower thanbiodiesel blend b20 by 6.5%, 16% and 23% but the exergetic efficiency are increased by 7%,19% and 30% at full load about b20.

关键词： Waste cooking oil(WCO) Nano additives Engine performance Exergy Combustion characteristics Emissions

Performance,emissions and exergy analyses of adding CNTs to various biodiesel feedstocks

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Propulsion and Power Research 2022年第4期11卷 511-526页

Extraction of biodiesel from waste cooking oil,jatropha,and corn oils is done by *** and biodiesel were blended at 20%volume ratio to make methyl *** doses of 25,50,and 100 mg/l,carbon nanotubes(CNTs)was mixed with biodiesel *** objective of the present research is to examine experimentally a diesel engine performance,combustion characteristics,exergy and emissions analyses with inclusion of nano additive to various methyl ester *** ester blend is enriched with CNTs as Jb20C100,Wb20C100 and Cb20C100 where the improvements in thermal efficiency are raised about biodiesel mixture by 9%,13%and 15%,*** of 100 ppm of CNTs to biodiesel blends achieves the greatest reductions in CO(14%,22%and 30%),HC(16%,20%and 25%),and smoke emissions(15%,19%and 23%)for Jb20C100,Wb20C100 and Cb20C100,*** comparing with b20,blending 100 ppm CNTs with Jb20,Cb20,and Wb20 obtained the highest increases in cylinder pressure of 3%,5%,and 10%,as well as the highest increases in heat release of 4%,7%,and 11%,*** downside of CNTs addition achieves a rise in NOx emissions by 10%,17%,and 22%for Jb20C100,Wb20C100,and Cb20C100,*** efficiency increases by 8%,19%,and 24%for b20T100,b20A100,and b20C100,*** index improvements achieve 1.5%,5%and 6.5%,for b20T100,b20A100,and b20C100,***20 with CNTs of 100 ppm is highly recommended for improving engine performance,combustion,and exergy characteristics with considerable emissions reduction.

关键词： biodiesel Carbon nanotubes(CNTs) Engine performance Exergy Combustion characteristics Emissions

Differences in Ecological and Genetic Adaptations between Salamandra infraimmaculata and Ommatotriton vittatus

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Open Journal of Animal Sciences 2024年第3期14卷 183-193页

作者： gad Degani MIGAL Galilee Research Institute Qiryat Shemona Israel Faculty of Sciences Tel-Hai Academic College Qiryat Shemona Israel

Israel is home to two species of amphibians belonging to distinct genera: Salamandra and Ommatotriton. They inhabit various regions, sometimes coexisting and in other instances dwelling separately across different areas, making their segregation challenging. This study compares the biological, ecological, and genetic traits of two species, the Near Eastern fire salamander Salamandra infraimmaculata and the southern banded newt Ommatotriton vittatus, to determine why O. vittatus thrives in a wider range of semi-arid habitats in central and southern Israel, whereas S. infraimmaculata predominantly occupies the coastal Mediterranean region in the north. Salamander larvae are typically found in streams, freshwater springs, and cave pools, whereas newt larvae inhabit winter pools and ponds exclusively. The developmental phase of salamander tadpoles extends over several months, whereas newt tadpoles spend a comparatively brief period in the water, from 1 to a few months. Notably, genetic disparities in the cytochrome b sequence in Israeli populations are more pronounced among newts than salamanders.

关键词： Ommatotriton vittatus Salamandra infraimmaculata biological Ecological Genetic Israel

Integrated Use of Organic and bio-fertilizers to Improve Yield and Fruit Quality of Olives Grown in Low Fertility Sandy Soil in an Arid Environment

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Phyton-International Journal of Experimental botany 2023年第6期92卷 1813-1829页

作者： bassam F.Alowaiesh M.M.gad Mohamed Saleh M.Ali biology Department College of ScienceJouf UniversitySakaka72341Saudi Arabia Horticulture Department Faculty of AgricultureZagazig UniversityZagazig44511Egypt Horticulture Department (pomology)Faculty of AgricultureSuez Canal UniversityIsmailia41522Egypt

Olive productivity should be improved through stimulating nutrition,particularly under poor fertility ***,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid *** a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers *** fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season *** *** bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table *** results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,k,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit *** fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized ***,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned ***,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral ferti

关键词： Organic and bio-fertilizers olives kalamata vegetative growth leaf mineral contents fruit quality

Suppression of NLRP3 inflammasome by ivermectin ameliorates bleomycin-induced pulmonary fibrosis

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2023年第8期24卷 723-733页

作者： Mai A.AbD-ELMAWLA Heba R.GHAIAD Enas S.gad kawkab A.AHMED Maha AbDELMONEM Department of biochemistry Faculty of PharmacyCairo UniversityCairo 11562Egypt Department of Pharmaceutical Sciences Faculty of PharmacyKing Faisal UniversityAl-Ahsa 31982Saudi Arabia Department of Pharmacology and Toxicology Faculty of PharmacySinai UniversityIsmailia 45511Egypt Department of Pathology Faculty of Veterinary MedicineCairo UniversityCairo 12211Egypt

Ivermectin is a US Food and Drug Administration(FDA)-approved antiparasitic agent with antiviral and anti-inflammatory *** recent studies reported the possible anti-inflammatory activity of ivermectin in respiratory injuries,its potential therapeutic effect on pulmonary fibrosis(PF)has not been *** study aimed to explore the ability of ivermectin(0.6 mg/kg)to alleviate bleomycin-induced biochemical derangements and histological changes in an experimental PF rat *** can provide the means to validate the clinical utility of ivermectin as a treatment option for idiopathic *** results showed that ivermectin mitigated the bleomycin-evoked pulmonary injury,as manifested by the reduced infiltration of inflammatory cells,as well as decreased the inflammation and fibrosis ***,ivermectin decreased collagen fiber deposition and suppressed transforming growth factor-β1(TGF-β1)and fibronectin protein expression,highlighting its anti-fibrotic *** study revealed for the first time that ivermectin can suppress the nucleotide-binding oligomerization domain(NOD)-like receptor family pyrin domain-containing protein 3(NLRP3)inflammasome,as manifested by the reduced gene expression of NLRP3 and the apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),with a subsequent decline in the interleukin-1β(IL-1β)*** addition,ivermectin inhibited the expression of intracellular nuclear factor-κb(NF-κb)and hypoxia‑inducible factor‑1α(HIF-1α)proteins along with lowering the oxidative stress and apoptotic ***,this study revealed that ivermectin could ameliorate pulmonary inflammation and fibrosis induced by *** beneficial effects were mediated,at least partly,via the downregulation of TGF-β1 and fibronectin,as well as the suppression of NLRP3 inflammasome through modulating the expression of HIF‑1αand NF-κb.

关键词： Intra-tracheal instillation Immunohistochemistry Transforming growth factor-β1(TGF-β1) Nuclear factor-κb(NF-κb) Lung fibrosis

DNA Variation of <i>Capoeta</i><i>damascina</i>(Valenciennes, 1842) in Three Rivers in Northern Israel

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Journal of biophysical Chemistry 2014年第3期5卷 107-117页

作者： gad Degani School of Science and Technology Tel-Hai Academic College Upper Galilee Israel MIGAL-Galilee Technology Center Kiryat Shmona Israel

The present study is in agreement with the hypothesis that the variation of ecological conditions in three rivers in northern Israel—the Dan, Hasbani and Hermon Rivers—affects the genetic variations of the species Capoeta damascina. Using mitochondrial DNA (mtDNA), cytochrome b gene (Cytb), 16S and nuclear DNA (nDNA), and Random Amplified Polymorphic DNA (RAPD), four different clusters were found in the Cytb of the Hasbani and Hermon Rivers and only two in the Dan River. Moreover, the clusters in the Hasbani River differed from those found in the Hermon River. A similar result was found when an analysis was made of a different sequence from five different haplotype frequencies using the MegAlign program, the lowest being in the Dan River (only two haplotypes) and the highest in the Hasbani River (four haplotypes). The analysis of molecular variance of Cytb and 16S (AMOVA) for individuals of C. damascina from eight populations in northern Israel showed significant differences between the rivers and the populations. The analysis by mitochondrial 16S of haplotype frequencies of C. damascina populations in the rivers in northern Israel was very low compared to Ctb. Sixteen different haplotypes were found in the different rivers: eight in the Hasbani River, seven in the Dan River and only five in the Hermon River.

关键词： 16S Capoeta damascina Cytochrome b Gene (Cytb) Mitochondrial DNA (mtDNA) Nuclear DNA (nDNA) Random Amplified Polymorphic DNA (RAPD)

Molecular overview of progressive familial intrahepatic cholestasis

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World Journal of Gastroenterology 2020年第47期26卷 7470-7484页

作者： Sriram Amirneni Nils Haep Mohammad A gad Alejandro Soto-Gutierrez James E Squires Rodrigo MFlorentino Department of Pathology University of PittsburghPittsburghPA 15213United States Pittsburgh Liver Research Center University of PittsburghPittsburghPA 15213United States Division of Gastroenterology Hepatologyand NutritionUPMC Children's Hospital of PittsburghPittsburghPA 15224United States

Cholestasis is a clinical condition resulting from the imapairment of bile *** condition could be caused by defects of the hepatocytes,which are responsible for the complex process of bile formation and secretion,and/or caused by defects in the secretory machinery of *** mutations and pathways that lead to cholestasis have been *** familial intrahepatic cholestasis(PFIC)is a group of rare diseases caused by autosomal recessive mutations in the genes that encode proteins expressed mainly in the apical membrane of the *** 1,also known as byler’s disease,is caused by mutations of the ATP8b1 gene,which encodes the familial intrahepatic cholestasis 1 *** 2 is characterized by the downregulation or absence of functional bile salt export pump(bSEP)expression via variations in the AbCb11 *** of the AbCb4 gene result in lower expression of the multidrug resistance class 3 glycoprotein,leading to the third type of *** variations of this disease have been *** of function of the tight junction protein 2 protein results in PFIC 4,while mutations of the NR1H4 gene,which encodes farnesoid X receptor,an important transcription factor for bile formation,cause PFIC 5.A recently described type of PFIC is associated with a mutation in the MYO5b gene,important for the trafficking of bSEP and hepatocyte membrane *** this review,we provide a brief overview of the molecular mechanisms and clinical features associated with each type of PFIC based on peer reviewed journals published between 1993 and 2020.

关键词： Progressive familial intrahepatic cholestasis ATP8b1/familial intrahepatic cholestasis 1 AbCb11/bile salt export pump AbCb4/multidrug resistance class 3 Intrahepatic cholestasis bile