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Axonal degeneration in the anterior insular cortex is associated with Alzheimer’s co-pathology in Parkinson’s disease and dementia with Lewy bodies

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Translational Neurodegeneration 2022年第1期11卷 129-147页

作者： Yasmine Y.Fathy Laura E.Jonkman John J.Bol Evelien Timmermans Allert J.Jonker annemieke J.M.Rozemuller Wilma D.J.van de Berg Amsterdam UMC Department of Anatomy and NeurosciencesSection Clinical Neuroanatomy and BiobankingAmsterdam NeuroscienceVrije University AmsterdamO|2 Life Sciences buildingDe Boelelaan 11081081 HZ Amster-damNetherlands Amsterdam Neuroscience Program NeurodegenerationAmsterdamthe Netherlands Amsterdam UMC Department of Pathol-ogyAmsterdam NeuroscienceVrije University AmsterdamDe BoelelaanAmsterdamNetherlands Department of Neurology Erasmus Medical CenterPostbus 20403000 CA RotterdamNetherlands.

Background:Axons,crucial for impulse transmission and cellular trafficking,are thought to be primary targets of neurodegeneration in Parkinson’s disease(PD)and dementia with Lewy bodies(DLB).Axonal degeneration occurs early,preceeding and exceeding neuronal loss,and contributes to the spread of pathology,yet is poorly described outside the nigrostriatal circuitry.The insula,a cortical brain hub,was recently discovered to be highly vulnerable to pathology and plays a role in cognitive deficits in PD and DLB.The aim of this study was to evaluate morphological features as well as burden of proteinopathy and axonal degeneration in the anterior insular sub-regions in PD,PD with dementia(PDD),and DLB.Methods:α-Synuclein,phosphorylated(p-)tau,and amyloid-βpathology load were evaluated in the anterior insular(agranular and dysgranular)subregions of post-mortem human brains(n=27).Axonal loss was evaluated using modified Bielschowsky silver staining and quantified using stereology.Cytoskeletal damage was comprehensively studied using immunofluorescent multi-labelling and 3D confocal laser-scanning microscopy.Results:Compared to PD and PDD,DLB showed significantly higherα-synuclein and p-tau pathology load,argyrophilic grains,and more severe axonal loss,particularly in the anterior agranular insula.Alternatively,the dysgranular insula showed a significantly higher load of amyloid-βpathology and its axonal density correlated with cognitive performance.p-Tau contributed most to axonal loss in the DLB group,was highest in the anterior agranular insula and significantly correlated with CDR global scores for dementia.Neurofilament and myelin showed degenerative changes including swellings,demyelination,and detachment of the axon-myelin unit.Conclusions:Our results highlight the selective vulnerability of the anterior insular sub-regions to various converging pathologies,leading to impaired axonal integrity in PD,PDD and DLB,disrupting their functional properties and potentially contributing to cognitive,emotional,and autonomic d

关键词： α-Synuclein Insular subregions Axonal length density Alzheimer’s disease pathology Neurofilament Myelin

Digestive oncologist in the gastroenterology training curriculum

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World Journal of Gastroenterology 2011年第9期17卷 1109-1115页

作者： Chris Jacob Johan Mulder Marc Peeters annemieke Cats Anna Dahele Jochim Terhaar sive Droste Department of Gastroenterology and Hepatology VU University Medical Center Department of Oncology Antwerp University Hospital Antwerp Department of Gastroenterology and Hepatology Netherlands Cancer Institute

Until the late 1980s, gastroenterology (GE) was considered a subspecialty of Internal Medicine. Today, GE also incorporates Hepatology. However, Digestive Oncology training is poorly defined in the Hepatogastroenterology (HGE)-curriculum. Therefore, a Digestive Oncology curriculum should be developed and this document might be a starting point for such a curriculum. HGE-specialists are increasingly resisting the paradigm in which they play only a diagnostic and technical role in the management of digestive tumors. We suggest minimum endpoints in the standard HGE-curriculum for oncology, and recommend a focus year in the Netherlands for Digestive Oncology in the HGE-curriculum. To produce welltrained digestive oncologists, an advanced Digestive Oncology training program with specific qualifications in Digestive Oncology (2 years) has been developed. The schedule in Belgium includes a period of at least 6 mo to be spent in a medical oncology department. The goal ofthese programs remains the production of well-trained digestive oncologists. HGE specialists are part of the multidisciplinary oncological teams, and some have been administering chemotherapy in their countries for years. In this article, we provide a road map for the organization of a proper training in Digestive Oncology. We hope that the World Gastroenterology Organisation and other (inter)national societies will support the necessary certifications for this specific training in the HGE-curriculum.

关键词：消化系统肿瘤培训课程胃肠病学肿瘤学消化道肿瘤国家管理肿瘤组织附件

Vitamin D_(3) attenuates anxiety-like behavior in long-term ovariectomized rats with unpredictable mild stress

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BIOCELL 2019年第4期43卷 299-311页

作者： DENIS BARANENKO JULIA FEDOTOVA annemieke JOHANNA MARIA VAN DEN TOL International Research Centre“Biotechnologies of the Third Millennium” ITMO University9 Lomonosova Str.St.Petersburg191002Russia Laboratory of Neuroendocrinology I.P.Pavlov Institute of Physiology RASci.6 Emb.MakarovaSt.Petersburg199034Russia University of Lincoln School of Psychology Sarah Swift Building 3201Brayford PoolLincolnLN57AYUK.

The impact of various vitamin D3(VD3)doses(1.0,2.5,or 5 mg/kg,s.c.)in mitigating the negative consequences of chronic unpredictable mild stress(CUMS)was investigated.Adult female rats with long-term estrogen deficiency were assessed using the sucrose preference test(SPT),the elevated plus-maze(EPM),the light/dark test(LDT),and the open-field test(OFT)to measure anhedonia-like and anxiety-like behavior.The corticosterone(CS)and adrenocorticotrophic hormone(ACTH)concentrations in blood serum and the brain-derived neurotrophic factor(BDNF)expression in the hippocampus of long-term ovariectomized(OVX)rats were measured by ELISA kits and/or western blotting.Treatment with VD3(5.0 mg/kg),similarly to fluoxetine(10.0 mg/kg),significantly reduced the anhedonia profile in the SPT and anxiety-like behavior in the EPM and LDT,and CS and ACTH levels in blood serum.It also elevated BDNF levels in the hippocampus of long-term OVX/CUMS compared to OVX/CUMS/solvent rats.Thus,these findings suggest that VD3(5.0 mg/kg)administration might attenuate the anxiety-like profile in long-term OVX adult rats subjected to the CUMS.This might occur via activation of the BDNF signaling pathway in the hippocampus and via restoration of CS and ACTH levels in blood serum.

关键词： Anxiety BDNF Chronic unpredictable mild stress Long-term ovariectomy Vitamin D3

Exposing hidden diversity by concordant genealogies and morphology—a study of the Lactifluus volemus(russulales)species complex in Sikkim Himalaya(India)

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Fungal Diversity 2012年第4期55卷 171-194页

作者： Kobeke Van de Putte Jorinde Nuytinck Kanad Das annemieke Verbeken Research group Mycology Department of BiologyUniversity of GhentK.L.Ledeganckstraat 359000GhentBelgium Botanical Survey of India Sikkim Himalaya Regional CentreP.O.RajbhawanGangtok 737103 SikkimIndia

Although Sikkim belongs to one of the Global Biodiversity Hotspots,little is known about its ectomycorrhizal fungi,and even less about the main genera of russulales,i.e.Lactarius,Lactifluus,Multifurca and russula.Combining a multilocus genealogical and morphological study,we aimed to document the diversity within Lactifluus volemus sensu lato of Sikkim Himalaya.We compared nuclear ITS and LSU rDNA,nuclear rpb1 and rpb2 protein-coding,and mitochondrial atp6 protein-coding genealogies to determine species boundaries.Interspecific relationships were inferred from the combined dataset.Bayesian and maximum likelihood single-locus genealogies are concordant and support recognition of six species.Three of these could be identified by unique morphological characteristics and are described as new species:L.dissitus,L.leptomerus and L.versiformis.

关键词： Cryptic species Genealogical concordance Multilocus approach Phylogenetic species russulaceae

Taxonomic revisionofthegenus Lactarius(russulales,Basidiomycota)in Korea

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Fungal Diversity 2019年第2期95卷 275-335页

作者： Hyun Lee Komsit Wissitrassameewong Myung Soo Park annemieke Verbeken John Eimes Young Woon Lim School of Biological Sciences and Institute of Microbiology Seoul National University1 Gwanak-roGwanak-guSeoul 08826Korea Department of Biology Research Group MycologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium University College Sungkyunkwan University2066 SeoburoJangan-guSuwon 16419Korea

The genus Lactarius Pers.(russulales)is a cosmopolitan group of Basidiomycota that forms ectomycorrhizal relationships primarily with both deciduous and coniferous trees.Although the genus has been well-studied in Europe and North America,only fragmentary researches have been carried out on Asian species.In particular,the distribution of Lactarius species in South Korea is poorly understood due to insufficient morphological descriptions and a lack of DNA sequence data.In addition,the misuse of European and North American names has added to confusion regarding the taxonomy of Asian Lactarius species.In this study,the diversity of Lactarius in South Korea was evaluated by employing both morphological and phylogenetic approaches.A multi-locus phylogenetic analysis of 729 Lactarius specimens collected between 1960 and 2017 was performed using the internal transcribed spacer(ITS)region,partial nuclear ribosomal large subunit(nrLSU),partial second largest subunit of RNA polymerase II(rpb2),and minichromosome maintenance complex component 7(mcm7).49 Lactarius species were identified in three Lactarius subgenera:L.subg.russularia(17 spp.),L.subg.Lactarius(22 spp.),and L.subg.Plinthogalus(10 spp.).Among them,28 Lactarius species were identified as new to science,while just 17 were previously described Lactarius species.Four of the taxa remain un-named due to paucity of materials.A key to Korean Lactarius species,molecular phylogenies,a summary of diversity,and detailed description are provided.

关键词： Intercontinental conspecificity mcm7 Milkcaps Multi-locus phylogeny Species delimitation

Effects of land use on the fungal spore richness in small crater-lake basins of western Uganda

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Fungal Diversity 2012年第4期55卷 125-142页

作者： Vanessa Gelorini annemieke Verbeken Luc Lens Hilde Eggermont Bent Vad Odgaard Dirk Verschuren Limnology Unit Department of BiologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium Research Group Mycology Department of BiologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium Terrestrial Ecology Unit Department of BiologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium Royal Belgian Institute of Natural Sciences Freshwater BiologyVautierstraat 291000 BrusselsBelgium Department of Geosciences University of AarhusHoegh Guldbergsgade 2DK-8000 Aarhus CDenmark Research Unit Palaeontology Department of Geology and Soil SciencesGhent UniversityKrijgslaan 281/S89000 GhentBelgium

Mycological tools to estimate the effects of diverse land-use practices on fungal diversity are scarce,because of poor knowledge of the taxonomic diversity of tropical fungi and their response to anthropogenic habitat change.Here,we investigate assemblages of fungal spores,recently deposited in the bottom sediments of 24 small crater lakes in western Uganda,to assess the relationship between the local richness of fungi and environmental variation in the crater basin along regional gradients of natural vegetation and land use.We recovered~9500 fungal spore specimens,which could be attributed to 216 morphotypes.Using an information-theoretic approach based on the corrected Akaike Information Criterion(AICc),we determined the environmental factors which best explained variation in the diversity of fungal spores among three datasets:(i)the full set of 24 crater basins,(ii)the subset of 22 basins with freshwater lakes,and(iii)the subset of 17 basins partly or completely in agricultural use(cropland,fallow land,pasture and plantation).In these 17 human-impacted crater basins our results revealed a negative relationship between fungal spore richness and the areal fraction of basins in agricultural use.However,this detrimental effect of land use on fungal spore richness was not apparent across the full set of both disturbed and(presently)undisturbed basins.This was due to large variation in fungal spore richness among the undisturbed basins covered either with forest or savannah vegetation,probably resulting from site-specific controls on fungal habitat diversity,such as climatic moisture balance and the composition of natural and/or secondary vegetation.The land-use effects on fungal spore diversity,as documented in this study,suggest that communities of tropical fungi progressively exposed to land-use practices are threatened by species loss.Hence,our study demonstrates the need to develop conservation strategies mitigating the impacts of agriculture on the biodiversity of tropical fungi.

关键词： Fungal spores Richness Land use Lake basins Western Uganda

The Australasian species of Lactarius subgenus Gerardii(russulales)

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Fungal Diversity 2012年第1期52卷 141-167页

作者： Dirk Stubbe Huyen Than Le Xiang-Hua Wang Jorinde Nuytinck Kobeke Van de Putte annemieke Verbeken Department of Biology Ghent UniversityK.L.Ledeganckstraat 359000 GhentBelgium Section Mycology and Aerobiology Scientific Institute of Public HealthJuliette Wytmanstraat 141050 BrusselsBelgium Faculty of Environment Hanoi University of Natural Resources and Environment41A Street K1Cau DienTu LiemHanoiVietnam Kunming Institute of Botany Chinese Academy of SciencesLanhei Road 132#Kunming 650204People’s Republic of China Research Group Mycology Department of BiologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium

This paper provides an overview and identification key of the Australasian species of Lactarius subg.Gerardii.A molecular phylogeny based on the nuc DNA markers ITS,LSU and rpb2,combined with detailed morphological observations resulted in the delimitation of 12 strongly supported and morphologically recognizable species.Five new agaricoid species are described here:Lactarius hora,L.coniculus,L.limbatus,L.leae and L.leonardii.Other agaricoid species belonging to subg.Gerardii are L.atrovelutinus,L.bicolor,L.ochrogalactus and L.reticulatovenosus.Lactarius venosus is also considered as a member but could not be included in the molecular analysis.Two new pleurotoid species are discovered:L.genevievae and L.conchatulus,both close relatives of L.uyedae,but each with distinct characters.Their position within subg.Gerardii is supported in the phylogeny and by their microscopic characters.This study also confirms the existence of multiple cryptic species and species complexes for which species recognition or species delimitation remains problematic as is the case for the Australian species L.wirrabara.In general,detailed macroscopic and microscopic observations are needed to identify species of L.subg.Gerardii.

关键词： Basidiomycota Lactifluus Plinthogalus Taxonomy Phylogeny Asia

Phosphorylated TDP-43 Staging of Primary Age-Related Tauopathy

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Neuroscience Bulletin 2019年第2期35卷 183-192页

作者： Xiaoling Zhang Bing Sun Xing Wang Hui Lu Fangjie Shao annemieke J.M.Rozemuller Huazheng Liang Chong Liu Jiadong Chen Manli Huang Keqing Zhu China Brain Bank and Department of Neurology in Second Affiliated Hospital Key Laboratory of Medical Neurobiology of Zhejiang ProvinceDepartment of NeurobiologyZhejiang University School of Medicine Department of Pathology Zhejiang University School of Medicine Department of Pathology Amsterdam NeuroscienceVU University Medical Center Brain Structure and Function Group Neuroscience Research Australia Department of Psychiatry First Affiliated HospitalZhejiang University School of Medicine

Primary age-related tauopathy(PART) is characterized by tau neurofibrillary tangles(NFTs) in the absence of amyloid plaque pathology. In the present study,we analyzed the distribution patterns of phosphorylated43-kDa TAR DNA-binding protein(pTDP-43) in the brains of patients with PART. Immunohistochemistry and immunofluorescence double-labeling in multiple brain regions was performed on brain tissues from PART,Alzheimer's disease(AD), and aging control cases. We examined the regional distribution patterns of pTDP-43 intraneuronal inclusions in PART with Braak NFT stages[ 0 and B IV, and a Thal phase of 0(no beta-amyloid present). We found four stages which indicated potentially sequential dissemination of pTDP-43 in PART. Stage I was characterized by the presence of pTDP-43 lesions in the amygdala, stage II by such lesions in the hippocampus,stage III by spread of pTDP-43 to the neocortex, and stage IV by pTDP-43 lesions in the putamen, pallidum, and insular cortex. In general, the distribution pattern of pTDP-43 pathology in PART cases was similar to the early TDP-43 stages reported in AD, but tended to be more restricted to the limbic system. However, there were some differences in the distribution patterns of pTDP-43 between PART and AD, especially in the dentate gyrus of the hippocampus. Positive correlations were found in PART between the Braak NFT stage and the pTDP-43 stage and density.

关键词： TDP-43 Primary age-related tauopathy Alzheimer's disease Neurofibrillary tangle Hippocampus

Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry

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World Journal of Gastroenterology 2006年第10期12卷 1536-1544页

作者： Judith YMN Engwegen Helgi H Helgason annemieke Cats Nathan Harris Johannes MG Bonfrer Jan HM Schellens Jos H Beijnen Department of Pharmacy and Pharmacology The Netherlands Cancer Institute/Slotervaart Hospital AmsterdamThe Netherlands Department of Medical Oncology The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Department of Gastroenterology and Hepatology The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Ciphergen Biosystems Inc. Freemont CaliforniaUnited States Department of Clinical Chemistry The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital AmsterdamThe Netherlands Utrecht University Faculty of Pharmaceutical Sciences Department of Biomedical analysisUtrechtThe Netherlands

瞄准：为颜色检测新浆液简历标记由浆液蛋白质与提高表面的激光解吸附作用 ionisation 介绍的表面的癌症(CRC )-- 飞行团分光术(SELDI-TOF MS ) 的时间。方法：二个独立浆液样品集合与 ProteinChip 技术独立被分析(集合 A：40 CRC+49 ... 详细信息

瞄准：为颜色检测新浆液简历标记由浆液蛋白质与提高表面的激光解吸附作用 ionisation 介绍的表面的癌症(CRC )-- 飞行团分光术(SELDI-TOF MS ) 的时间。方法：二个独立浆液样品集合与 ProteinChip 技术独立被分析(集合 A：40 CRC+49 健康控制；集合 B：37 CRC+31 健康控制) ，有一个弱阳离子的使用的芯片交换一半和缓冲区 pH 5。差别的歧视的力量表示了蛋白质与一个分类树算法被估计。产生分类树的敏感和特性被盲目地从集合 B 并且反过来也如此从集合 A 把数据用于产生的树获得。CRC 浆液蛋白质侧面也与从胸的那些相比，卵巢，前列腺，和非小的房间肺癌症。结果：Mass-to-charge 比率(m/z ) 3.1x10 (3 ) ， 3.3x10 (3 ) ， 4.5x10 (3 ) ， 6.6x10 (3 ) 和 28x10 (3 ) 在最好执行的分类树上被用作分类器。树敏感和特性在 65% 和 90% 之间。大多数这些歧视的 m/z 价值在调查的另外的瘤类型也是不同的。M/z 3.3x10 (3 ) ，在大多数树上的主要分类器，是 6.6x10 (3 ) 的一种二倍地控告的形式 -Da 蛋白质。后者作为 apolipoprotein C-I 被识别。M/z 3.1x10 (3 ) 作为 apolipoprotein A-I 作为白朊，和 m/z 28x10 (3 ) 的 N 终端碎片被识别。结论：分类树模式分析跟随的 SELDI-TOF MS 是为为 CRC 发现新浆液标记的一种合适的技术。Biomarkers 能与高敏感和特性在独立样品集合被识别并且 reproducibly 检测了。尽管为 CRC 不特定，这些简历标记在监视的疾病和治疗有一个潜在的角色。

关键词：血清蛋白结肠癌光谱测定检查方法