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Therapeutic advances in neural regeneration for Huntington’s disease

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Neural Regeneration Research 2024年第9期19卷 1991-1997页

作者： Francesco D’Egidio Vanessa Castelli Giorgia Lombardozzi Fabrizio Ammannito annamaria Cimini Michele d’Angelo Department of Life Health and Environmental SciencesUniversity of L’AquilaL’AquilaItaly

Huntington’s disease is a neurodegenerative disease caused by the expansion mutation of a cytosine-adenine-guanine triplet in the exon 1 of the HTT gene which is responsible for the production of the huntingtin (Htt) protein. In physiological conditions, Htt is involved in many cellular processes such as cell signaling, transcriptional regulation, energy metabolism regulation, DNA maintenance, axonal trafficking, and antiapoptotic activity. When the genetic alteration is present, the production of a mutant version of Htt (mHtt) occurs, which is characterized by a plethora of pathogenic activities that, finally, lead to cell death. Among all the cells in which mHtt exerts its dangerous activity, the GABAergic Medium Spiny Neurons seem to be the most affected by the mHtt-induced excitotoxicity both in the cortex and in the striatum. However, as the neurodegeneration proceeds ahead the neuronal loss grows also in other brain areas such as the cerebellum, hypothalamus, thalamus, subthalamic nucleus, globus pallidus, and substantia nigra, determining the variety of symptoms that characterize Huntington’s disease. From a clinical point of view, Huntington’s disease is characterized by a wide spectrum of symptoms spanning from motor impairment to cognitive disorders and dementia. Huntington’s disease shows a prevalence of around 3.92 cases every 100,000 worldwide and an incidence of 0.48 new cases every 100,000/year. To date, there is no available cure for Huntington’s disease. Several treatments have been developed so far, aiming to reduce the severity of one or more symptoms to slow down the inexorable decline caused by the disease. In this context, the search for reliable strategies to target the different aspects of Huntington’s disease become of the utmost interest. In recent years, a variety of studies demonstrated the detrimental role of neuronal loss in Huntington’s disease condition highlighting how the replacement of lost cells would be a reasonable strategy to o

关键词： cell therapy exosomes extracellular vesicles Huntingtin Huntington’s disease medium spiny neurons neurodegenerative disease neurogenesis neuronal loss stem cells

细菌新生RNA捕获及转录延伸速率检测方法的开发与应用(英文)

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生物化学与生物物理进展 2024年

作者：李媛媛王雨婷武子淳李昊轩费明月孙东昌 Gualerzi O.Claudio Fabbretti Attilio Giuliodori annamaria 马红霞贺承光吉林农业大学生命科学技术学院教育部生物反应器与药物开发工程研究中心卡梅里诺大学生物科学与兽医学院浙江工业大学生物技术与生物工程学院

目的：检测和定量细胞中RNA的合成是一种广泛应用于监测细胞活力、健康和代谢率的技术。在环境刺激下，内参基因和靶基因都会发生一定程度的降解。因此，在受到环境刺激后，必须考虑对新生RNA的精确捕获和RNA转录水平的检测。方法：使... 详细信息

目的：检测和定量细胞中RNA的合成是一种广泛应用于监测细胞活力、健康和代谢率的技术。在环境刺激下，内参基因和靶基因都会发生一定程度的降解。因此，在受到环境刺激后，必须考虑对新生RNA的精确捕获和RNA转录水平的检测。方法：使用5-乙基尿苷(5-EU)对新生RNA进行标记，利用“点击化学”和磁珠筛选相结合的方法，将叠氮化物-生物素介质配体与磁球连接，随后捕获新生RNA并进行荧光分子信标(M.B.)和qRT-PCR方法检测16S rRNA转录速率。结果：本研究采用了“点击化学”和磁珠筛选相结合的RNA捕获方法，优化了革兰氏阴性菌，特别是大肠杆菌的转录速率检测方法。结论：与其他文章报道的利用磁珠筛选的实验方法相比，本研究采用的技术方案更适合细菌，操作步骤简单，易于实现，是研究人员有效的RNA捕获方法。

关键词：新生RNA筛选点击化学荧光分子信标

Detrital zircon ages of Oligocene to Miocene sandstone suites of the Southern Apennines foreland basin system,Italy

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Journal of Palaeogeography 2022年第2期11卷 222-237页

作者： annamaria Fornelli Francesca Micheletti Salvatore Gallicchio Fabrizio Tursi Sara Criniti Salvatore Critelli Dipartimento Scienze Della Terra e Geoambientali Universit a Degli Studi“Aldo Moro”BariVia E.Orabona470125BariItal Dipartimento di Biologia Ecologia e Scienze Della TerraUniversit a Della CalabriaVia Pietro Bucci87036Arcavacata di RendeCosenzaItaly

U-Pb spot ages on detrital zircons from turbidite quartzarenites,quartz-feldspathic and volcaniclastic sandstones of the Tufiti di Tusa(TTF),Numidian Flysch(NF),Bifurto(BF),Gorgoglione(GF)and Serra Palazzo(SPF)formations,exposed in southern Italy,were detected to define their provenance signatures aiming to contribute to reconstructing the complex geodynamic evolution of the southern Apennine *** deposition ages of these sandstones range from Rupelian(TTF),Burdigalian p.p.-Early Langhian(NF and BF)and Langhian-Serravallian-Early Tortonian(GF and SPF).The detrital zircon ages in each deposit show different *** TTF,the zircon age clusters are:610±6 Ma;390±4 Ma;287±3 Ma;157±2 Ma;78±1 Ma and 67±1 Ma;33±1 *** NF and BF formations,the prevalent zircon ages are clustered at 661±10 Ma,607±8 Ma and 547±8 *** GF sandstones,the ages of detrital zircons are clustered around 672±28 Ma,458±9 Ma,297±8 Ma and 24±1 *** sandstones of SPF contain zircons showing age clusters at649±10 Ma,486±7 Ma and 315±5 *** and lithic fragments from TTF display a dominant provenance from Oligocene calc-alkaline effusive products located on western domains of the Mesomediterranean Microplate,and minor detritus from granitoids and metamorphic rocks of Variscan *** of NF and BF indicate a provenance of detritus from the African Craton without evidence of Variscan or Alpine *** from GF and SPF show a provenance from both Variscan crystalline basement rocks forming the Alpine chain(Calabria-Peloritani Terranes)and Variscan magmatic and metamorphic rocks unaffected by Alpine tectonics,similar to Sardinia-Corsica(pro-parte)*** addition,the Oligocene magmatic component dated at 24 Ma in GF sandstones is evidence for the maximum age of sedimentation for these *** light of the recent and preliminary geochronological data acquired,a revised paleogeographic restoration could be inferred for the location of sedimentary basins during the Apennines t

关键词： U-Pb detrital Zircon ages Provenance of sandstones Southern Apennine chain Southern Circum-Mediterranean paleogeography

Blue light enhanced Heck arylation at room temperature applied to allenes

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Organic Chemistry Frontiers 2022年第4期9卷 906-916页

作者： Polyssena Renzi Emanuele Azzi Enrico Bessone Giovanni Ghigo Stefano Parisotto Francesco Pellegrino annamaria Deagostino Department of Chemistry University of TorinoVia Pietro Giuria710125 TorinoItaly

An unprecedented visible light enhanced room temperature Heck reaction between aryl halides and allenyl tosyl amines is here reported.A simple catalytic system(Pd(OAc)_(2)/PPh_(3))is employed to afford arylated vinyl pyrrolidines and piperidines.A broad scope with high tolerance towards functional groups is *** effects play an important role in the efficiency of this *** studies,both experimental and computational,indicate no evidence for a radical mechanism and a pivotal role of light in promoting the carbo-palladation step.

关键词： process light Heck

Butein-instigated miR-186-5p-dependent modulation of TWIST1 affects resistance to cisplatin and bioenergetics of Malignant Pleural Mesothelioma cells

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Cancer Drug Resistance 2022年第3期5卷 814-828页

作者： Mario Cioce Daniela Rutigliano annamaria Puglielli Vito Michele Fazio Department of Medicine Laboratory of Molecular Medicine and BiotechnologyUniversity Campus Bio-Medico of RomeRome 00128Italy Institute of Translational Pharmacology National Research Council of Italy(CNR)Rome 00133Italy

Aim:Malignant pleural mesothelioma is a chemoresistant tumor,and biphasic and sarcomatoid histologies portend the worst prognosis for malignant pleural mesothelioma(MPM)*** obtained the microRNA expression profile of three biphasic-sarcomatoid MPM cell lines to identify commonly expressed microRNAs and evaluate the effect of butein,a chemo-sensitizing compound,on this microRNA ***:Nanostring-based microRNA profiling and analysis through the ROSALIND platform were employed to identify the commonly modulated microRNAs and their ***-mimic transfection,Luciferase assay,and Western blotting were employed to show specific perturbation of TWIST1 levels by ***-forming assays,invasion assay,and metabolic profiling were used to assess the biological consequences of the butein-instigated miR-186-5p-mediated perturbation of TWIST1 *** analysis was used to search for the correlation between TWIST1 and miR-186-5p levels in biphasic and epithelioid MPM ***:We identified a set of perturbed microRNAs,common to three biphasic/sarcomatoid MPM cell lines,after butein *** focusing on miR-186-5p,we unraveled a butein-ignited and miR-186-5p-mediated modulation of TWIST1 levels which affected the 3D anchorage-independent growth,cisplatin resistance,invasion,and bioenergetics of the MPM cell lines *** showed that miR-186-5p and TWIST1 levels are anti-correlated in biphasic MPM specimens from ***:We unraveled a novel mechanism of action of butein,which attenuated the pro-tumorigenic features of MPM at least through a miR-186-5p-TWIST1 *** suggest that those activities converge into the chemo-sensitizing effect of this compound and may be of translational relevance.

关键词： Mesothelioma butein miR-186-5p TWIST1 pithelial-to-mesenchymal transition(EMT) chemoresistance cancer metabolism invasion anchorage-independent growth

AdipoRon and Pancreatic Ductal Adenocarcinoma:a future perspective in overcoming chemotherapyinduced resistance?

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Cancer Drug Resistance 2022年第3期5卷 625-636页

作者： Luigi Sapio Angela Ragone annamaria Spina Alessia Salzillo Silvio Naviglio Department of Precision Medicine University of Campania“Luigi Vanvitelli”Naples 80138Italy

The latest scientific knowledge has provided additional insights accountable for the worst prognosis for pancreatic ductal adenocarcinoma(PDAC).Among the causative factors,the aptitude to develop resistance towards approved medications denotes the master key for understanding the lack of improvement in PDAC survival over the *** though several compounds have achieved encouraging results at preclinical stage,no new adjuvant agents have reached the bedside of PDAC patients *** adiponectin receptor agonist AdipoRon is emerging as a promising anticancer drug in different cancer models,particularly in *** on the existing findings,we recently reinforced its candidacy in PDAC cells,proposing AdipoRon either as a suitable partner in gemcitabinebased treatment or as an effective drug in resistant *** the current state-of-the-art,herein we provide a critical perspective on AdipoRon to figure out whether this receptor agonist can potentially be considered a future therapeutic choice in overcoming chemotherapy-induced resistance,expressly in PDAC.

关键词： PDAC AdipoRon gemcitabine resistance

Giant retroperitoneal liposarcoma treated with radical conservative surgery: A case report and review of literature

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World Journal of Clinical Cases 2022年第19期10卷 6636-6646页

作者： Eva Lieto Francesca Cardella Silvia Erario Giovanni Del Sorbo Alfonso Reginelli Gennaro Galizia Fabrizio Urraro Iacopo Panarese annamaria Auricchio Department of Traslational Medical Sciences University of Campania"L.Vanvitelli"Naples 80128CampaniaItaly Department of Precision Medicine University of Campania"L.Vanvitelli"Naples 80138CampaniaItaly Depatment of Pathology Unit-Menthal Health University of Campania"L.Vanvitelli"Naples 80132CampaniaItaly

BACKGROUND Retroperitoneal liposarcoma(RLPS)is a rare malignant tumor of the connective tissue and usually grows to a large size,*** is currently based on collective findings from clinical examinations and computed tomography(CT)and magnetic resonance imaging,the latter of which show a fat density mass and possible surrounding organ *** resection is the main therapeutic *** efficacy and safety of further therapeutic choices,such as chemotherapy and radiotherapy,are still *** SUMMARY A 61-year-old man presented with complaint of a large left inguinal mass that had appeared suddenly,after a slight *** revealed an omental inguinal *** further clinical examination,an enormous palpable abdominal mass,continuing from the left inguinal location,was *** revealed a giant RLPS,with remarkable mass effect and wide visceral *** multidisciplinary consultation,surgical intervention was *** neoadjuvant chemotherapy and radiotherapy were precluded by the mass’large size and retroperitoneal localization,features typically associated with non-response to these types of ***,the patient underwent conservative treatment via radical surgical *** 1 year,his clinical condition remained good,with no radiological signs of *** Conservative treatment via surgery resulted in a successful outcome for a large RLPS.

关键词： Retroperitoneal liposarcoma Giant sarcoma Sarcoma surgical treatment Sarcoma diagnosis Differentiated liposarcoma Retroperitoneal sarcoma surgical outcome Case report

From post-COVID-19-associated myocarditis to hemopericardium: a dangerous domino effect

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Journal of Geriatric Cardiology 2023年第5期20卷 391-396页

作者： Francesco Bellanti Ripalta Amato Antonio Centola Valeria Ercolano Lucia Barbera annamaria Tesse Grazia Divittorio Cristiano Capurso Aurelio Lo Buglio Gianluigi Vendemiale Department of Medical and Surgical Sciences University of Foggiaviale Pinto 171122 FoggiaItaly Department of Internal and Geriatric Medicine Policlinico Riunitiviale Pinto 171122 FoggiaItaly Department of Cardiology Policlinico Riunitiviale Pinto 171122 FoggiaItaly

The majority of patients infected with Severe Acute Respiratory Syndrome-COrona-Virus-2(SARS-CoV-2)either completely recover from symptoms in a few days or stay asymptomatic.[1−3]Nevertheless,a consistent proportion of subjects with a history of probable or confirmed SARS-CoV-2 infection refers persistency,new occurrence,relapse,or fluctuation of symptoms,and these manifestations are defined as post-COVID-19 condition.[4]Of note,the post-acute sequelae of COVID-19 include several cardiac manifestations,including direct myocardial and pericardial injury/inflammation,as well as cardiomyopathies and arrythmias,sustained by viral infiltration and/or dysregulation of adaptive immune response.[5]Even though rare,acute myocarditis may be an important post-COVID-19 condition and may be associated with the occurrence of new-onset atrial fibrillation.

关键词： myocarditis acute sustained

Hepatic steatosis,low-grade chronic inflammation and hormone/growth factor/adipokine imbalance

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World Journal of Gastroenterology 2010年第38期16卷 4773-4783页

作者： Giovanni Tarantino Silvia Savastano annamaria Colao Department of Clinical and Experimental Medicine Federico Ⅱ University Medical School of Naples Department of Molecular and Clinical Endocrinology and Oncology Section of EndocrinologyFederico Ⅱ University Medical School of Naples

Non-alcoholic fatty liver disease (NAFLD), a further expression of metabolic syndrome, strictly linked to obesity and diabetes mellitus, is characterized by insulin resistance (IR), elevated serum levels of free fatty acids and fatty infi ltration of the liver, which is known as hepatic steatosis. Hepatocyte apoptosis is a key feature of this disease and correlates with its severity. Free-fatty-acidinduced toxicity represents one of mechanisms for the pathogenesis of NAFLD and hormones, growth factors and adipokines influence also play a key role. This review highlights the various pathways that contribute to the development of hepatic steatosis. Circulating concentrations of inflammatory cytokines are reckoned to be the most important factor in causing and maintaining IR. Low-grade chronic inflammation is fundamental in the progression of NAFLD toward higher risk cirrhotic states.

关键词： Hepatic steatosis Low-grade chronic inflammation Adipokines Hormones Growth factors