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Gastroesophageal reflux disease management according to contemporary international guidelines:A translational study

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World Journal of Gastroenterology 2011年第9期17卷 1160-1166页

作者： Fabio Pace Gabriele Riegler annalisa de Leone Patrizia Dominici Enzo Grossi the EMERGE Study Group Division of Gastroenterology Department of Clinical Sciences 'L. Sacco' UniversityHospital 74-20157 Milano Italy 'Magrassi-Lanzara' Department of Clinicaland Experimental Medicine 2nd University of Naples 80100Naples Italy Medical Department BraccoImaging SpA 24100 Milano Italy

AIM:To test the Genval recommendations and the usefulness of a short trial of proton pump inhibitor(PPI) in the initial management and maintenance treatment of gastroesophageal reflux disease(GERD)patients. METHODS:Five hundred and seventy seven patients with heartburn were *** completing a psychometric tool to assess quality of life(PGWBI)and a previously validated GERD symptom questionnaire (QUID),patients were grouped into those with esophagitis(EE,n=306)or without mucosal damage (NERD,n=271)according to endoscopy results. The study started with a 2-wk period of high dose omeprazole(omeprazole test);patients responding to this PPI test entered an acute phase(3 mo)of treatment with any PPI at the standard ***,those patients with a favorable response to the standard PPI dose were maintained on a half PPI dose for a further 3-mo period. RESULTS:The test was positive in 519(89.9%)patients,with a greater response in EE patients(96.4%) compared with NERD patients(82.6%)(P=0.011). Both the percentage of completely asymptomatic patients,at 3 and 6 mo,and the reduction in heartburn intensity were significantly higher in the EE compared with NERD patients(P<0.01).Finally,the mean PGWBI score was significantly decreased before and increased after therapy in both subgroups when compared with the mean value in a reference Italian population. CONCLUSION:Our study confirms the validity of the Genval guidelines in the management of GERD patients. In addition,we observed that the overall response to PPI therapy is lower in NERD compared to EE patients.

关键词： Gastroesophageal reflux disease Proton pump inhibitors Nonerosive gastroesophageal reflux Questionnaire Quality of life

Role of Long Non-coding RNAs in Reprogramming to Induced Pluripotency

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Genomics, Proteomics & Bioinformatics 2020年第1期18卷 16-25页

作者： Shahzina Kanwal Xiangpeng Guo Carl Ward Giacomo volpe Baoming Qin Miguel A.Esteban Xichen Bao Joint School of Life Sciences Guangzhou Medical University and Guangzhou Institutes of Biomedicine and HealthGuangzhou 511436China Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Laboratory of RNA Chromatinand Human DiseaseGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou 510005China Laboratory of Metabolism and Cell Fate Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cells and Regeneration Chinese Academy of SciencesBeijing 100101China Laboratory of RNA Molecular Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China

The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular *** reorganization occurs in a multi-phased manner and involves a gradual revision of both the epigenome and *** studies have shown that the large-scale transcriptional changes observed during reprogramming also apply to long noncoding RNAs(lncR NAs),a type of traditionally neglected RNA species that are increasingly viewed as critical regulators of cellular *** understanding of lncR NAs in reprogramming may not only help to improve this process but also have implications for studying cell plasticity in other contexts,such as development,aging,and *** this review,we summarize the current progress made in profiling and analyzing the role of lncR NAs in various phases of somatic cell reprogramming,with emphasis on the re-establishment of the pluripotency gene network and X chromosome reactivation.

关键词： Mesenchymal-to-epithelial transition X chromosome reactivation Apoptosis Proliferation

Giant cavernous liver hemangiomas： is it the time to change the size categories？

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Hepatobiliary & Pancreatic Diseases International 2016年第1期15卷 21-29页

作者： isidoro di carlo renol koshy saif al mudares annalisa ardiri gaetano bertino adriana toro departments of general surgery hamad general hospitalDohaQatar department of surgical sciences and advanced technologies"g.f.ingrassia" university of cataniaCataniaItaly hepatology unit department of medical and pediatric scienceuniversity of cataniaCataniaItaly department of surgery patti hospitalMessinaItaly

BACKGROUND： Four different sizes （4, 5, 8 and 10 cm in diameter） can be found in the literature to categorize a liver hemangioma as giant. The present review aims to clarify the appropriateness of the size category ＂giant＂ for liver heman- gioma. DATA SOURCES： We reviewed the reports on the categoriza- tion of hemangioma published between 1970 and 2014. The number of hemangiomas, size criteria, mean and range of hemangioma sizes, and number of asymptomatic and symp- tomatic patients were investigated in patients aged over 18 years. Liver hemangiomas were divided into four groups：〈5.0 cm, 5.0-9.9 cm, 10.0-14.9 cm and 〉15.0 cm in diameter. Inclu- sion criteria were noted in 34 articles involving 1972 （43.0%） hemangiomas （〉4.0 cm）. RESULTS： The patients were divided into the following groups： 154 patients （30.0%） with hemangiomas less than 5.0 cm in diameter （small）, 182 （35.5%） between 5.0 cm and 9.9 cm （large）, 75 （14.6%） between 10.0 and 14.9 cm （giant）, and 102 （19.9%） more than 15.0 cm （enormous）. There were 786 （39.9%） asymptomatic patients and 791 （40.1%） symptomatic patients. Indications for surgery related to symptoms were reported in only 75 （3.8%） patients. Operations including 137 non-anatomical resection （12.9%） and 469 enudeation （44.1%） were undearly related to size and ***： The term ＂giant＂ seems to be justified for liver hemangiomas with a diameter of 10 cm. Hemangiomas categorized as ＂giant＂ are not indicated for surgery. Surgery should be performed only when other symptoms are apparent.

关键词： giant hemangioma cavernous hemangioma liver hemangioma

MicroRNAs as controlled systems and controllers in non-alcoholic fatty liver disease

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World Journal of Gastroenterology 2014年第41期20卷 15079-15086页

作者： Nadia Panera Daniela Gnani annalisa Crudele Sara Ceccarelli Valerio Nobili Anna Alisi Hepato-Metabolic Disease Unit "Bambino Gesù" Children’s HospitalIRCCS Liver Research Unit "Bambino Gesù" Children’s HospitalIRCCS

Non-alcoholic fatty liver disease(NAFLD) is a multi-faceted condition including simple steatosis alone or associated with inflammation and ballooning(non-alcoholic steatohepatitis) and eventually *** NAFLD incidence has increased over the last twenty years becoming the most frequent chronic liver disease in industrialized ***,visceral adiposity,insulin resistance,and many other disorders that characterize metabolic syndrome are the major predisposing risk factors for ***,different factors,including genetic background,epigenetic mechanisms and environmental factors,such as diet and physical exercise,contribute to NAFLD development and *** lines of evidence demonstrate that specific microRNAs expression profiles are strongly associated with several pathological conditions including *** NAFLD,microRNA deregulation in response to intrinsic genetic or epigenetic factors or environmental factors contributes to metabolic *** this review we focused on microRNAs role both as controlled and controllers molecules in NAFLD development and/or their eventual value as non-invasive biomarkers of disease.

关键词： Liver steatosis Fibrosis Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis MicroRNAs

In vitro effects of polyphenols on colorectal cancer cells

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World Journal of Gastrointestinal Oncology 2014年第8期6卷 289-300页

作者： Barbara Pampaloni Gaia Palmini Carmelo Mavilia Roberto Zonefrati annalisa Tanini Maria Luisa Brand Department of Surgery and Translational Medicine University of Florence Florence 50139 Italy

AIM:To investigate the effects of quercetin and genistein on colon cancer cell proliferation and their estrogen receptorβ(ERβ)***:Colon cancer cells were stably transfected with a mammalian expression vector to overexpress ERβ(HCT8-β8-expressing cells)or a control vector(HCT8-pSV2neo-expressing cells).The proliferation of these cells was examined after treatment with quercetin or genistein(5-100μmol/L),or 10 nmol/L17β-estradiol(17β-E2).Cell viability was examined by acridine orange staining following treatments for 48 or144 *** of quercetin and genistein on ERβtranscriptional transactivation were examined by luciferase activity in HCT8-β8-expressing cells transiently transfected with a pEREtkLUC reporter *** addition,the regulation of ERβtranscription by phytoestrogens and 17β-E2 was examined by quantitative polymerase chain ***:Proliferation of HCT8-β8-expressing cells was not reduced low doses(5μmol/L)of quercetin and genistein,while it was reduced at 25-50μmol/L with an effect similar to 10 nmol/L 17β-*** with doses of phytoestrogens≥75μmol/L completely blocked cell growth and reduced overall cell counts,however no effects at any dose were observed in HCT8-pSV2neoexpressing *** results were supported by viability staining that revealed acridine orange-stained lysosomes with high doses or extended treatment *** and quercetin(50μmol/L)significantly increased ER-responsive luciferase activity similar to 10nmol/L 17β-E2(P<0.05).Furthermore,genistein and quercetin(50μmol/L),as well as 10 nmol/L 17β-E2significantly increased ERβmRNA levels in HCT8-β8-expressing cells(P<0.05).In addition,treatment of HCT8-pSV2neo-expressing cells with 50μmol/L quercetin or 10 nmol/L 17β-E2 significantly increased ERβmRNA levels compared to untreated controls(P<0.05),though the absolute levels were much lower than in HCT8-β8-expressing ***:The antitumorigenic effects of the phytoestrogenic compounds querc

关键词： Estrogen receptor HCT8-β8 cells HCT8pSV2neo Quercetin Genistein

Dendritic cell subsets in cancer immunity and tumor antigen sensing

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Cellular & Molecular Immunology 2023年第5期20卷 432-447页

作者： annalisa Del Prete Valentina Salvil Alessandra Soriani Mattia Laffranchi Francesca Sozio Daniela Bosisio Silvano Sozzani Department of Molecular and Translational Medicine University of BresciaBresciaItaly Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti Department of Molecular Medicine Sapienza University of RomeRomeItaly

Dendritic cells(DCs)exhibit a specialized antigen-presenting function and play crucial roles in both innate and adaptive immune *** to their ability to cross-present tumor cell-associated antigens to naïve T cells,DCs are instrumental in the generation of specific T-cell-mediated antitumor effector responses in the control of tumor growth and tumor cell *** an immunosuppressive tumor microenvironment,DC antitumor functions can,however,be severely *** this review,we focus on the mechanisms of DC capture and activation by tumor cell antigens and the role of the tumor microenvironment in shaping DC functions,taking advantage of recent studies showing the phenotype acquisition,transcriptional state and functional programs revealed by scRNA-seq *** therapeutic potential of DC-mediated tumor antigen sensing in priming antitumor immunity is also discussed.

关键词： Dendritic cell subsets Tumor microenvironment Migration Innate immune sensing Immune cell death Tumor-derived extracellularvesicles

Radioisotope production using lasers:From basic science to applications

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Matter and Radiation at Extremes 2024年第3期9卷 31-44页

作者： M.R.D.Rodrigues A.Bonasera M.Scisciò J.A.Pérez-Hernández M.Ehret F.Filippi P.L.Andreoli M.Huault H.Larreur D.Singappuli D.Molloy D.Raffestin M.Alonzo G.G.Rapisarda D.Lattuada G.L.Guardo C.Verona Fe.Consoli G.Petringa A.McNamee M.La Cognata S.Palmerini T.Carriere M.Cipriani G.Di Giorgio G.Cristofari R.De Angelis G.A.P.Cirrone D.Margarone L.Giuffrida D.Batani P.Nicolai K.Batani R.Lera L.volpe D.Giulietti S.Agarwal M.Krupka S.Singh Fa.Consoli Cyclotron Institute Texas A&M UniversityCollege StationTexas 77840USA Laboratori Nazionali del Sud Istituto Nazionale di Fisica Nucleare(LNS-INFN)CataniaItaly ENEA Fusion and Technologies for Nuclear Safety and Security Department-CFrascatiItaly Centro de Láseres Pulsados(clpu) VillamayorSpain Universidad de Salamanca SalamancaSpain Centre Lasers Intenses et Applications(CELIA) Universitéde BordeauxCNRSCEATalenceFrance HB11 Energy Holdings Pty FreshwaterNew South WalesAustralia School of Mathematics and Physics Queen’s University BelfastBelfastUnited Kingdom Dipartimento di Fisica e Astronomia“E.Majorana ”Università di CataniaCataniaItaly Facoltàdi Ingegneria e Architettura Universitàdegli Studi di Enna“Kore”EnnaItaly Dipartimento di Ingegneria Industriale Universitàdi Roma“Tor Vergata”RomaItaly Dipartimento di Fisica e Geologia Universitàdegli Studi di PerugiaPerugiaItaly Istituto Nazionale di Fisica Nucleare Sezione di PerugiaPerugiaItaly ELI Beamlines Facility the Extreme Light Infrastructure ERICDolni BrezanyCzech Republic ETSIA Universidad Politécnica de MadridMadridSpain Dipartimento Fisica “E.Fermi”Universitàdi Pisa and INFNPisaItaly FZU–Institute of Physics of Czech Academy of Sciences PragueCzech Republic Institute of Plasma Physics of Czech Academy of Sciences PragueCzech Republic

The discovery of chirped pulse amplification has led to great improvements in laser technology,enabling energetic laser beams to be compressed to pulse durations of tens of femtoseconds and focused to a few *** with energies of tens of MeV can be accelerated using,for instance,target normal sheath acceleration and focused on secondary *** such conditions,nuclear reactions can occur,with the production of radioisotopes suitable for medical *** use of high-repetition lasers to produce such isotopes is competitive with conventional methods mostly based on *** this paper,we study the production of^(67)Cu,^(63)Zn,^(18)F,and^(11)C,which are currently used in positron emission tomography and other *** the same time,we study the reactions^(10)B(p,α)^(7)Be and^(70)Zn(p,4n)^(67)Ga to put further constraints on the proton distributions at different angles,as well as the reaction^(11)B(p,α)^(8)Be relevant for energy *** experiment was performed at the 1 PW laser facility at VegaⅢin Salamanca,*** distributions of radioisotopes in the forward(with respect to the laser direction)and backward directions were measured using a high purity germanium *** results are in reasonable agreement with numerical estimates obtained following the approach of Kimura and Bonasera[*** ***.,Sect.A 637,164–170(2011)].

关键词： purity estimates backward

The Unexpected Existence of Coding and Non-Coding Fragments along the Eukaryotic Gene

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Advances in Biological Chemistry 2015年第2期5卷 98-125页

作者： Pietro volpe Department of Biology University of Rome “Tor Vergata” Rome Italy National Institute of Biostructures and Biosystems Rome Italy

The pathways leading to synthesis and post-synthetic modification of DNA employed methionine as donor of atoms: the carbon that came from its –CH3 served for DNA replication and repair either in bacteria or humans;its entire –CH3 served instead for building N6-methyladenine and 5-methylcytosine on bacterial DNA and 5-methylcytosine alone on human DNA. In humans, although a slight extra-S asymmetric methylation appeared de novo yielding on parental DNA 5’-m5CpC-3’/ 3’-GpG-5’, 5’-m5CpT-3’/3’-GpA-5’ and 5’-m5CpA-3’/3’-GpT-5’ monomethylated dinucleotide pairs, a heavy symmetric methylation involved in S semiconservatively newly made DNA to guarantee genetic maintenance of –CH3 in 5’-m5CpG-3’/3’-Gpm5C-5’ dimethylated dinucleotide pairs. In this framework, an inverse correlation was found between bulk genomic DNA methylation occurring in S and bulk polyA-containing pre-mRNA transcription taking place in G1 and G2. Thus, probes of 1 × 106 Daltons (constructed using sheared by sonication newly made methylated DNA filaments) revealed a modular organization in genes: after the hypermethylated promoter, they exhibited an alternation of unmethylated coding and methylated uncoding sequences. This encouraged the search for a language that genes regulated by methylation should have in common. An initial deciphering of restriction minimaps with hypomethylatable exons vs. hypermethylatable promoters and introns was improved when the bisulfite technique allowed a direct sequencing of m5C. In lymphocytes, where the transglutaminase gene is inactive, its promoter exhibited two fully methylated CpG-rich domains at 5’ and one fully unmethylated CpG-rich domain at 3’, including the site +1 and a 5’-UTR. At variance, in HUVEC cells, where the transglutaminase gene is active, in the first CpG-rich domain of promoter few doublets lost their –CH3. Such an inverse correlation suggested new hypotheses especially in connection with repair-modification: UV radiation would cause demethylation in g

关键词： Coding vs. Non-Coding Pre-Messenger RNA Regions Exons and Introns Multigenic and Monogenic Transcriptional Units Regulation of Gene Expression Repair-Modification

Chemokine and chemotactic signals in dendritic cell migration

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Cellular & Molecular Immunology 2018年第4期15卷 346-352页

作者： Laura Tiberio annalisa Del Prete Tiziana Schioppa Francesca Sozio Daniela Bosisio Silvano Sozzani Department of Molecular and Translational Medicine University of BresciaBresciaItaly Humanitas Clinical and Research Institute Rozzano-MilanoItaly

Dendritic cells (DCs) are professional antigen-presenting cells responsible for the activation of specific T-cell responses and for thedevelopment of immune tolerance. Immature DCs reside in peripheral tissues and specialize in antigen capture, whereas matureDCs reside mostly in the secondary lymphoid organs where they act as antigen-presenting cells. The correct localization of DCs isstrictly regulated by a large variety of chemotactic and nonchemotactic signals that include bacterial products, DAMPs (dangerassociated molecular patterns), complement proteins, lipids, and chemokines. These signals function both individually and inconcert, generating a complex regulatory network. This network is regulated at multiple levels through different strategies, such assynergistic interactions, proteolytic processing, and the actions of atypical chemokine receptors. Understanding this complexscenario will help to clarify the role of DCs in different pathological conditions, such as autoimmune diseases and cancers and willuncover new molecular targets for therapeutic interventions.

关键词： generating network mostly